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Biomedical subjects

M Horiguchi

Publications and source records attributed to M Horiguchi.

At least 199 records · Page 11Linked to original sources
PubMed1980

Metabolism of 2-amino-3-phosphono[3-14C]propionic acid in cell-free preparations of Tetrahymena.

Incubation of 2-amino-3-phosphono[3-14C]propionic acid with cell-free preparations of Tetrahymena pyriformis resulted in incorporation of the radioactivity into phosphoenolpyruvate. No radioactivity was incorporated into phosphoenolpyruvate and 2-phosphonoacetate. Thus, the catabolic pathway of 2-amino-3-phosphonopropionic acid in Tetrahymena appears to be the same route as that found in rats through deamination to produce 3-phosphonopyruvate which is converted to 2-phosphonoacetaldehyde by decarboxylation, followed by both dephosphonylation and amination of the aldehyde to give acetaldehyde and 2-aminoethylphosphonic acid, respectively.

Acetaldehyde↗
PubMed1980

Nutritional Interdependence Among Rumen Bacteria, Bacteroides amylophilus, Megasphaera elsdenii, and Ruminococcus albus.

Nutritional interdependence among three representatives of rumen bacteria, Bacteroides amylophilus, Megasphaera elsdenii, and Ruminococcus albus, was studied with a basal medium consisting of minerals, vitamins, cysteine hydrochloride, and NH(4). B. amylophilus grew well in the basal medium supplemented with starch and produced branched-chain amino acids after growth ceased. When cocultured with B. amylophilus in the basal medium supplemented with starch and glucose, amino acid-dependent M. elsdenii produced an appreciable amount of branched-chain fatty acids, which are essential growth factors for cellulolytic R. albus. A small addition of starch (0.1 to 0.3%) to the basal medium containing glucose and cellobiose brought about successive growth of the three species in the order of B. amylophilus, M. elsdenii, and R. albus, and successive growth was substantiated by the formation of branched-chain amino acids and fatty acids in the culture. Supplementation with 0.5% starch, however, failed to support the growth of R. albus. On the basis of these results, the effects of supplementary starch or branched-chain fatty acids on cellulose digestion in the rumen was discussed.

Journal Article↗
PubMed1980

The cutaneous branch of some human suprascapular nerves.

A cutaneous branch of the suprascapular nerve was observed in 6 arms from 5 (4 male and 1 female) out of 61 Japanese cadavers. The suprascapular nerves with a cutaneous branch arose from essentially normal brachial plexuses. Every suprascapular nerve with a cutaneous branch had a normal course, and gave rise to the cutaneous branch either from the upper of its two muscular branches to the supraspinatus or from its stem under the superior transverse scapular ligament. After passing between the coracoclavicular and coracoacromial ligaments the cutaneous branch pierced the deltoid muscle close to the tip of the acromion. In one case, the peripheral distribution was very carefully dissected, the nerve supplied the proximal third of the lateral aspect of the arm within the territory of the axillary nerve. A cutaneous branch of the suprascapular nerve is obviously not uncommon, at least in Japanese.

Aged↗
PubMed1979

Metabolism of 2-amino-3-phosphono[3-14C]propionic acid in cell-free preparations of rat liver.

Incubation of 2-amino-3-phosphono[3-14C]propionic acid with cell-free preparations of rat liver yielded labelled 3-phosphonopyruvic acid, 2-phosphonoacetaldehyde, 2-aminoethylphosphonic acid and acetaldehyde. No radioactivity was found in phosphoenolpyruvate, pyruvic acid, alanine, and phosphonoacetic acid. When added to the cell-free preparations, 3-phosphonopyruvic acid trapped the radioactivity, resulting in decrease of incorporation of the radioactivity into 2-phosphonoacetaldehyde, 2-aminoethylphosphonic acid and acetaldehyde. Incorporation of the radioactivity into 2-aminoethylphosphonic acid and acetaldehyde was also decreased by 2-phosphonoacetaldehyde. Thus it appears that the main metabolic pathway of 2-amino-3-phosphonopropionic acid is deamination to produce 3-phosphonopyruvic acid which is, in turn, converted to 2-phosphonoacetaldehyde by decarboxylation, followed by both dephosphonylation and amination of the aldehyde to give acetaldehyde and 2-aminoethylphosphonic acid, respectively.

Alanine↗
PubMed1979

Metabolism of 2-amino-3-phosphonopropionic acid in rats.

The metabolism of 2-amino-3-phosphono-[2-(14)C]propionic acid or 2-amino-3-phosphono-[3-(14)C]propionic acid in rats was studied in vivo and in vitro. The radioactivity in expired CO2 from the [3-(14)C]-labelled compound indicated the cleavage of the carbon-phosphorus (C-P) bond. A small amount of the [2-(14)C]-labelled compound and the [3-(14C]-labelled compound was incorporated into 2-aminoethylphosphonic acid, and polar lipid of the liver and kidney contained the 2-aminoethylphosphonic acid. The 2-amino-3-phosphonopropionic acid was not detected at the lipid level. Incorporation of the [3-(14)C]-labelled compound into a variety of metabolites including 3-phosphonopyruvic acid and 2-phosphonoacetaldehyde suggests the transamination reaction as a decomposition mechanism of 2-amino-3-phosphonopropionic acid in mammals.

Alanine↗
PubMed1975

Phosphonopyruvic acid: A probable precursor of phosphonic acids in cell-free preparation of Tetrahymena.

1. In cell-free preparations of Tetrahymena, doubly labelled [32P]phosphoenol-[3-14C]pyruvate gives rise to 2-aminoethylphosphonate and 2-amino-3-phosphonopropionate, labelled with the two isotopes in the same ratio as the starting compound. The result is consistent with an intra-molecular rearrangement of phosphoenolpyruvate in the biosynthetic sequence of carbon-phosphorus bond formation. 2. Incubation of [32P]phosphoenolpyruvate with the same preparation, followed by treatment with 2,4-dinitrophenylhydrazine, yielded labelled hydrazones. When these were subjected to hydrogenolysis, the radioactivity was recovered in 2-aminoethylphosphonate and 2-amino-3-phosphonopropionate, suggesting that 2-phosphonoacetaldehyde and 3-phosphonopyruvic acid were probable precursors of the aminoalkylphosphonic acids. 3. Radioactivity from 2-amino-3-phosphono-[3-14C]propionic acid was incorporated into 2-aminoethylphosphonic acid, but incorporation of the radioactivity into lipids was negligible.

Animals↗
PubMed1975

Complications of valvular surgery--5 cases report with special reference to the conduction system--.

Five interesting autopsy cases of post-valvular replacement death are reported and discussed with special reference to disturbances of the conduction system. The most important acute changes are hemorrhages; the significance of a venous hemorrhage is emphasized. Chronic changes are collagenization of conduction system. One case of mycotic valvulitis, probably due to aspergillus, and one case of dissecting aneurysm probably related to previous valve replacement surgery are included.

Adult↗