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Biomedical subjects

M Horiba

Publications and source records attributed to M Horiba.

82 records · Page 5Linked to original sources
PubMed1985

[Analysis of human sera obtained from lung cancer patients by two-dimensional electrophoresis after schizophyllan (SPG) treatment].

Schizophyllan (SPG) was administered to 13 lung cancer patients (i.m. 20mg X 2/week) for 3 weeks without chemo or irradiation therapies, and serum proteins were analyzed by two-dimensional electrophoresis (TDE). Additionally, immunosuppressive acidic protein (IAP) was quantitatively determined by single radial immunodiffusion (SRID). By TDE analysis, human serum proteins were separated into more than 100 spots, and about 14 spots were found to show quantitative changes in cancer patients. Quantitative examination was therefore conducted on changes of 8 components among these spots, including alpha 1-acidic glycoprotein (alpha 1 AG), acidic alpha 2-macroglobulin (acidic alpha 2 M), haptoglobin (Hp) and IAP. The protein which showed the most marked decrease in cancer patients, located between transferrin and IgG on the above TDE patterns, was ascertained to have a molecular weight of about 150,000 using a gel filtration method. This protein was increased in 7 of 13 patients after SPG treatment.

Adenocarcinoma↗
PubMed1984

Pharmacokinetic studies of mabuterol, a new selective beta 2-stimulant. I: Studies on the absorption, distribution and excretion in rats.

Pharmacokinetic studies on absorption, distribution, excretion and placental transfer of the beta-sympathomimetic 14C-labelled dl - 1 - (4 - amino - 3 - chloro - 5 - trifluoromethyl-phenyl)-2-tert.-butylamino-ethanol hydrochloride (mabuterol) have been carried out in rats. Mabuterol was easily absorbed from the whole length of the small intestine in a study in situ with the gastro-intestinal ligated loop technique. The blood and tissue levels of radioactivity reached the maximum within 1 h after oral administration. Concerning tissue distribution (excluding the digestive tract) after oral administration, the radioactivity in the liver, lung, kidney and several secretory organs was considerably higher than that in the blood. The levels of radioactivity in these tissues continued at almost the same levels for 2-6 h after oral administration, resulting from a slow gastric emptying of the radioactivity. The whole-body autoradiograms obtained after intravenous administration revealed the secretion of the radioactivity into the stomach. The radioactive concentration in the CNS was much lower than that in the principal organs with either route of administration. About 60% and 26% were excreted into urine and feces, respectively, within 24 h after oral administration. Although about 22% was excreted into bile within 24 h after intravenous administration, no other evidence of enterohepatic circulation was observed. The multiple oral administration of 14C-mabuterol indicated no, appreciable accumulation in the tissues of rats. A placental transfer in the pregnant rats in the last stage of gestation was not significant.

Adrenergic beta-Agonists↗
PubMed1984

Pharmacokinetic studies of mabuterol, a new selective beta 2-stimulant. II: Urinary metabolites of mabuterol in rats and their pharmacological effects.

A selective beta 2-stimulating bronchodilator, dl-1- (4-amino-3-chloro-5-trifluoromethyl-phenyl)-2-tert.-butyl-amino-ethanol hydrochloride (mabuterol), is mainly metabolized by oxidative deamination pathway. Unchanged mabuterol and the following 6 types of metabolites, M-1 (hydroxylation of tert.-butyl group), M-2 (glycol), M-3 (mandelic acid), M-4 (aldehyde), M-5 (benzoic acid) and M-6 (hippuric acid) were identified by thin-layer chromatography and mass spectroscopy. Pharmacologically, only M-1 had effects on the airway resistance, blood pressure and heart rate, and the isolated organs. But its selective beta 2-stimulating effect was 2-10 times lower than that of mabuterol. M-1 antagonized the positive inotropic action induced by isoprenaline (isoproterenol). Other metabolites showed no appreciable activity.

Adrenergic beta-Agonists↗