Search PubMed⌕ Search

Biomedical subjects

M Honma

Publications and source records attributed to M Honma.

At least 163 records · Page 9Linked to original sources
PubMed1985

Double-blind study with liposteroid in rheumatoid arthritis.

A multicentre double-blind comparative trial was performed in 138 patients with rheumatoid arthritis (RA) after biweekly intravenous or intramuscular injections of liposteroid (containing 2.5 mg of dexamethasone), which had been developed as a drug for targeting therapy of RA, and Decadron (containing 3.3 mg of dexamethasone) as a reference drug. The results showed a tendency to a significantly higher rate of improvement with lower frequency of side-effects in the liposteroid group than in the Decadron group. This study indicates that liposteroid is more useful for RA and that the separation of the efficacy and side-effects of steroids could be clinically confirmed to some extent.

Arthritis, Rheumatoid↗
PubMed1985

Cardiac effects of fenoterol hydrobromide on newborn versus adult rats.

The toxicity of fenoterol hydrobromide, a beta-adrenoceptor stimulant, was studied in newborn and adult SD-rats dosed with 0 (control), 2.5, 75 and 600 mg/kg/day by gavage for 35 days. 600 mg/kg was lethal for both age groups: newborn rats died either from gastro-intestinal disorders during the lactation period or from underweight and cachexia immediately after weaning. Adult rats which died from 600 mg/kg showed extended myocardial scars. No substance-related myocardial lesions were observed in newborn rats killed terminally, whereas adult rats had a dose-dependent increase in heart weights, at 600 mg/kg also extended myocardial scars.

Aging↗
PubMed1985

[A case of liver metastasis from a testicular tumor indicated by unreasonable CT findings--correlation of CT and autopsied specimen].

A 48-year-old men died of liver metastases from a left testicular tumor and was autopsied. It is thought to be rare for a pure seminoma of the testis to metastasize to the liver in the form of a large solitary nodule and for no obvious difference to be seen on plain and enhanced CT in the low-density area. These findings are unreasonable in view of the characteristic proliferative patterns of the tumor. Thus, the correlation between CT and the autopsied specimen was studied histopathologically with the purpose of understanding the focal conditions. The following results were obtained. A large low-density area in the right lobe of the liver on CT coincided with the autopsied specimen, and small nodular lesions scattered in the necrotic tissues were seen. The lesion was diagnosed to be metastasis from the testicular tumor and it was almost the coagulative necroses of the tumor cells in the histologic examination. Moreover, severe tumor thrimbi were observed in the blood vessels with special reference to metastatic foci. Above findings indicating the articular ischemic condition in the region of metastatic foci made it possible for the CT findings to be reasonably understood.

Dysgerminoma↗
PubMed1984

The product of gene secC is involved in the synthesis of exported proteins in E. coli.

To obtain additional mutants in the secretory apparatus of E. coli we have isolated suppressors of a mutant (secAts) that is temperature-sensitive for secretion. One of these, secC, can suppress the secretion defect of secA and has a phenotype of its own. At 23 degrees C, the secC mutant is cold-sensitive for growth and blocks the synthesis of transported proteins. The synthesis of at least one secreted protein, maltose-binding protein (MBP), can be restored by mutations that alter the hydrophobic region of the signal sequence of MBP. The phenotype of the secC mutant suggests that the SecC protein may be a component of the secretory apparatus of E. coli; it also supports the notion that in procaryotes secretion and gene expression are coupled. The secC gene maps at 68.5 minutes on the E. coli chromosome.

Amino Acid Sequence↗
PubMed1984

[Three cases of primary lung and mediastinal malignant germ cell tumors --clinical and pathological studies].

This is a clinical and pathological study of malignant germ tumors in the lung or mediastinum. Germ cell tumors may be considered to originate from primitive germ cells with totipotency. One of 3 cases showed clinical and pathological findings suggesting that a part of the tumor differentiated into many kinds of tissue, such as growing and metastasizing. Initial diagnosis was performed radiographically and pathologically with difficulty. But finally, the first patient was diagnosed as malignant teratoma, the second as yolk sac tumor and the third as choriocarcinoma, in consideration of the tumor markers and clinical process.

Adult↗
PubMed1984

A multicenter double-blind controlled study of lobenzarit, a novel immunomodulator, in rheumatoid arthritis.

A multicenter double-blind study was carried out in patients with rheumatoid arthritis (RA) by comparing treatment with a novel immunomodulator, lobenzarit, disodium 4-chloro-2, 2'-iminodibenzoate, with an inert placebo. Both groups of patients received 75 mg/day of indomethacin as a basal regimen during the study period of 16 weeks. Group 1 (115 patients) received 240 mg/day lobenzarit, 80 mg TID, and Group 2 (115 patients) received placebo TID orally. A statistically significant improvement was noted in the number of swollen joints and in the Lansbury index at Weeks 12 and 16 in Group 1 as compared to Group 2. Overall clinical effectiveness was significantly higher in Group 1 (63%) than in Group 2 (43%). Incidence of side effects was 38% in Group 1 and 22% in Group 2. The most frequent side effect in both groups was gastrointestinal upset. Our data confirm that lobenzarit is a useful agent in the treatment of patients with RA.

Arthritis, Rheumatoid↗
PubMed1983

Plasma cyclic nucleotide responses to psychological stress in normal and neurotic subjects.

The mirror drawing test (MDT) was performed to induce acute psychological stress in 10 normal volunteers and 23 neurotic patients. Plasma cAMP and cyclic guanosine 3',5'-monophosphate (cGMP) were determined serially before, during, and after the test. In controls, the MDT caused a significant increase in the plasma cAMP level, whereas no change was observed in plasma cGMP. This increase was suppressed by simultaneous administration of propranolol, although it was not affected by simultaneous injection of phentolamine. In neurotic patients, the instruction for the MDT itself resulted in increased cAMP and cGMP levels, although there were no further significant increases during and after the MDT. The results indicate: 1) the increase in plasma cAMP during the MDT reflects a beta-adrenergic stimulation; 2) in neurotics, the response of cAMP and cGMP to the MDT is different from the controls. This difference may be a potential parameter in the diagnosis and discrimination of neurotic disorders.

Adolescent↗
PubMed1983

Plasma cyclic AMP responses to adrenaline administration in patients with spinocerebellar degeneration, bronchial asthma, and diabetes mellitus--a preliminary report of a clinical test for detecting beta-adrenergic dysfunctions.

Plasma cyclic AMP responses to adrenaline administration in normal volunteers, patients with spinocerebellar degeneration, bronchial asthma, pulmonary emphysema, and diabetes mellitus were studied. Intramuscular administration of low doses (0.1--0.4 mg/person) of adrenaline caused a dose-dependent increase in plasma cyclic AMP. The increase in cyclic AMP was completely prevented by propranolol, while it was not affected by phentolamine or atropine. In patients with spinocerebellar degeneration, the concentrations of plasma cyclic AMP both before and after adrenaline administration were lower than in normal subjects. In asthmatic patients, the plasma cyclic AMP increase after adrenaline administration was smaller than that of the healthy controls. The plasma concentration of cyclic AMP in patients with insulin-dependent diabetes reached the peak level more slowly than in diabetic patients with dietary control alone. Examining changes in the plasma cyclic AMP level after adrenaline administration appears to be a useful means for assessing the degree of beta-adrenergic dysfunction.

Adult↗
PubMed1983

[Clinical evaluation of cefotaxime in internal medicine].

Cefotaxime (CTX) was used for 129 cases in respiratory tract and other infections; 57 cases of pneumonia, 20 cases of bronchopneumonia, 20 cases of acute bronchitis, 14 cases of chronic bronchitis, 7 cases of acute exacerbation of bronchiectasia or pulmonary emphysema, 4 cases of suppurative diseases of the lung, 1 case of pyothorax, 1 case of retropharyngeal abscess, 3 cases of pleurisy and 1 case of urinary tract infection. (A case was excepted on clinical evaluation.) CTX was administered by intravenous injection or drip infusion at a daily dose ranging from 0.5 to 8 g, for a term of 2 to 61 days. The total dose patients received ranged from 3 to 226 g. The results obtained were as follows. Clinical effects; excellent in 45 cases, good in 63 cases, fair in 9 cases, poor in 7 cases and unevaluable in 4 cases. The efficacy rate was 87.1% (108/124). Bacteriological effects; eliminated in 30 cases, decreased in 8 cases, unchanged in 2 cases and replaced in 1 case. The elimination rate was 75.6% (31/41). Side effects and abnormal laboratory findings; general itching, fatigue in lower extremities and albuminuria in 1 case each, and anemia in 2 cases. Increased number of eosinophiles and elevated GOT in 1 case each, elevated GOT and GPT in 3 cases and elevated GOT, GPT and A1-P in 2 cases. These symptoms or abnormal laboratory findings disappeared after the discontinuation or termination of CTX therapy. In view of the above, CTX may be considered to be a clinically useful antibiotic against respiratory tract infections.

Adult↗
PubMed1983

A double-blind comparison of phenylbutazone and suxibuzone, a prodrug of phenylbutazone, in rheumatoid arthritis.

One hundred and fifty patients with rheumatoid arthritis received suxibuzone (426 mg/day, equivalent to 300 mg of phenylbutazone), a prodrug of phenylbutazone, or phenylbutazone (300 mg/day) in a six-week double-blind comparison study. After six weeks of treatment, morning stiffness, joint symptoms, and grip strength all improved almost equally in both groups. On the other hand, the frequency and severity of side-effects, particularly of gastro-intestinal (GI) disturbances, were markedly and significantly lower in the suxibuzone group. This study indicates that some prodrugs of non-steroid anti-inflammatory drugs are useful because they have fewer side-effects.

Anti-Inflammatory Agents↗
PubMed1982

Distribution pattern of lysosomal granules in fibroblasts of the Chediak-Higashi syndrome.

Cultured fibroblasts from a patient with the Chediak-Higashi syndrome, the mother of the patient, and a normal control were studied by light and electron microscopy. The distribution pattern of PAS-positive and acid phosphatase-containing granules in the cytoplasm differed significantly in the fibroblasts from the patient when compared with those from the mother and control. The granules in the fibroblasts from the patient were clustered in the perinuclear area, whereas the granules in the fibroblasts from the mother and control were dispersed throughout the cytoplasm. After incubation with ascorbic acid, the clustered granules in the fibroblasts of the Chediak-Higashi syndrome showed a tendency to spread throughout the cytoplasm. The distribution pattern of the granules was studied by quantitative morphology.

Ascorbic Acid↗
PubMed1982

Plasma guanosine 3',5'-monophosphate responses to methacholine and epinephrine in humans.

The im injection of methacholine into healthy volunteers caused a dose-dependent increase in plasma cGMP levels; this increase was antagonized by atropine, while it was not affected by phentolamine or propranolol. The im injection of epinephrine caused a small but significant rise in plasma cGMP concentrations; this rise was completely blocked by the simultaneous injection of phentolamine, while it was not affected by atropine or propranolol. These data show that changes in the plasma concentration of cGMP may reflect not only cholinergic but also alpha-adrenergic functions in humans.

Adolescent↗
PubMed1981

Mechanistic studies on the pyridoxal phosphate enzyme 1-aminocyclopropane-1-carboxylate deaminase from Pseudomonas sp.

The enzyme 1-aminocyclopropane-1-carboxylate deaminase (ACPC deaminase) from a pseudomonad is a pyridoxal phosphate (PLP) linked catalyst which fragments the cyclopropane substrate to alpha-ketobutyrate and ammonia [Honma, M., & Shimomura, T. (1978) Agric. Biol. Chem. 42, 1825]. Enzymatic incubations in D2O yield alpha-ketobutyrate with one deuterium at the C-4 methyl group and one deuterium at one of the C-3 prochiral methylene hydrogens. Stereochemical analysis of the location of the C-3 deuteron was accomplished by in situ enzymatic reduction to (2S)-2-hydroxybutyrate with L-lactate dehydrogenase and conversion to the phenacyl ester. The C-3 hydrogens of the (2S)-2-hydroxybutyryl moiety are fully resolved in a 250-MHz NMR spectrum. Absolute assignment of 3S and 3R loci was obtained with phenacyl (2S,3S)-2-hydroxy[3-2H]butyrate generated enzymatically by D-serine dehydratase action on D-threonine. ACPC deaminase shows a stereoselective outcome with a 3R:3S deuterated product ratio of 72:28. 2-Vinyl-ACPC is also a fragmentation substrate with exclusive regiospecific cleavage to yield the straight-chain keto acid product 2-keto-5-hexenoate. The D isomer of vinylglycine is processed to alpha-ketobutyrate and ammonia at 8% the Vmax of ACPC, while L-vinylglycine is not a substrate. It is likely that ACPC and D-vinylglycine yield a common intermediate--the vinylglycine-PLP-p-quinoid adduct--which is then protonated sequentially at C-4 and then C-3 to account for the observed deuterium incorporation. The D isomers of beta-substituted alanines (fluoroalanine, chloroalanine, and O-acetyl-D-serine) partition between catalytic elimination and enzyme inactivation. Each shows a different partition ratio, arguing against the common aminoacrylyl-PLP as the inactivating species.

Butyrates↗