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Biomedical subjects

M Holub

Publications and source records attributed to M Holub.

At least 73 records · Page 4Linked to original sources
PubMed1983

Large granular lymphocytes in blood of healthy donors and patients with tumors.

The percentage of large granular lymphocytes (LGL) in the peripheral blood of patients with less advanced tumors (T1 and T2) averaged 2.15 +/- 1.3 as compared with 3.8 +/- 1.5 in patients with more progressive tumors (T3 and T4) and with metastases. The values of LGL remained constant as long as 6 weeks both in the diseased and healthy subjects and were not age- nor sex-related. This observation confirms the usefulness and reliability of the test as one of the markers of the immunologic state since LGL are considered to represent NK cells responsible for resistance to malignant growth.

Adult↗
PubMed1982

Specific unresponsiveness to sheep red blood cells visualized by levamisole in athymic nude mice.

The administration of levamisole at the time of and 2 days after challenge with 10(8) SRBC significantly increased the direct anti-SRBC PFC response of nude mice. Pretreatment of nude mice of BALB/c and C 57 B1/10Sn strains with 10(9) or 10(8) SRBC injected 14 and 7 days before challenge suppressed the increase of the immune response. Pretreatment with 10(7) SRBC did not induce this inhibition. Administration of levamisole at the time of pretreatment with SRBC did not prevent the induction of inhibition. It is suggested that B cell exhaustion is the cause of the observed unresponsiveness.

Animals↗
PubMed1982

Detection of human antinuclear antibodies on rodent omenta.

Detection of antinuclear antibodies in the sera of patients by immunofluorescence using mouse or rabbit omentum is recommended, because artifacts stemming from cryostat sectioning and thawing the tissue are eliminated and the advantage of tissue-bound evenly spread cells is preserved. Moreover, the technique is sensitive and permits reading on easily recognizable cell types.

Animals↗
PubMed1981

Ontogeny of lymphatic structures in the pig omentum.

The development of lymphoid populations in the omentum majus during the prenatal and postnatal life of the pig was studied. T lymphocytes, monocytes and mast cells were first found on the 40th day of gestation. B lymphocytes appeared on the 72nd day of gestation when the first macrophage aggregates were formed. Macrophages appeared to be the prerequisite for the formation of dense lymphatic areas (DLA's). At later stages T cells were observed only in the omentum of germfree pigs. DLA's of conventional pig omentum are filled exclusively with B cells.

Animals↗
PubMed1980

Response of blood leucocytes to an intraperitoneal immunization in normal mice and leucopenic mice with the nu gene.

The response of leucocytes to an intraperitoneal injection of sheep red blood cells was investigated in normal (+/+) mice, athymic (nu/nu) mice and their hybrids (nu/+). In +/+ mice (in addition to a non-specific decrease in the number of granulocytes in the first 6 h after injection) the leucocyte levels were below the initial levels almost throughout the experiment (12 days). This was due mainly to lymphocytopenia. In nu/+ and nu/nu mice a marked leucocytosis developed, due mainly to the increased numbers of lymphocytes. The response of lymphocytes estimated by the morphological appearance of nucleoli showed an increase in numbers of both active lymphocytes with RNA-synthesizing nucleoli and terminal lymphocytes with micronucleoli.

Animals↗
PubMed1979

Skin-window study on the migration of leukocytes of newborns and infants.

Migration of leukocytes of newborns and of infants up to the age of 6 months was studied using the in vivo skin-window technique according to Rebuck. Using the non-specific stimulation (abrasion of the skin only) a slight age-dependent physiological increase of migration of cells was observed within the observation period; after a strong local irritation with diphtheria-tetanus-pertussis vaccine (Alditepera) there was a vigorous migratory response of cells in the skin lesion. Both quantitatively and qualitatively, the migration pattern (i.e. shift from PMN leukocyte to mononuclear cells in the exudate within a 1 d period after abrasion) was not influenced by immunization of infants with Alditepera, suggesting thus the nonspecific nature of this cellular response. The "normal" values of the chemotactic response of leukocytes of newborns and infants are given as a basis for evaluation of defects of this functional activity of leukocytes.

Age Factors↗
PubMed1979

Detection of thymosin 5 in calf and mouse thymus and in nude mouse dysgenetic thymus.

Thymosin 5 was traced in calf and mouse thymuses by fluorochrome-labelled rabbit anti-calf thymosin. The presence of thymosin 5 or its individual components was found 1. in groups of cortical epithelial cells in calf thymuses and in single cortical epithelial cells in mouse thymuses. 2. In some marginal (blastema) cells of the thymus cortex of calves and 14-day-old mice. 3. In perivascular epithelial cells of the calf thymus. 4. In occasional medullary epithelial cells of the calf and mouse thymus. In all cases there was a marked alternation of entirely negative and positive cell-containing thymic lobuli. In 4 of 13 cases comparatively strong positivity was found in tightyly arranged epithelial cells in an individual acinus in the dysgenetic thymus of nude mice, the positive cases being concentrated among the youngest mice studied.

Animals↗
PubMed1979

Cellular sources of thymic hormone (thymosin) in human thymus.

Antiserum against partially purified calf thymosin (5) was used for the localisation of this complex material in human thymus for comparison with calf thymi. Antithymosine IgG produced with human thymosin 5 by comparative immunoelectrophoresis one line identical with one of the lines of calf thymosin 5. Immunofluorescent evidence suggests the presence of the material in question, which also contains a biologically active principle, mainly in the epithelial cells of the thymus cortex. The contents of the traced material, however, fluctuates substantially between individual thymic lobules.

Animals↗