Search PubMed⌕ Search

Biomedical subjects

M Hilgarth

Publications and source records attributed to M Hilgarth.

At least 37 records · Page 2Linked to original sources
PubMed1987

Human papillomavirus standardization and DNA cytophotometry in cervical intraepithelial neoplasia.

Human papillomavirus (HPV) standardization and DNA cytophotometry were carried out in 29 cases of cervical intraepithelial neoplasia (CIN) grades I-III. A prognostically unfavorable DNA distribution pattern with an aneuploid stemline was found in 14 of the 16 dysplasias with HPV types 16 and 18, while in 11 of 13 dysplasias with HPV types 6 and 11 there was a favorable DNA distribution with a euploid-polyploid stemline. Among 178 colposcopically, cytologically and histologically confirmed cervical lesions, there was a statistically significant incidence of HPV 16/18 infections in severe dysplasias and carcinomas, while HPV 6/11 was found predominantly in mild cervical lesions. It seems that CIN can be divided into high- and low-risk lesions not only by the degree of severity and the DNA distribution pattern but also by HPV typing.

Adult↗
PubMed1986

[Significance of intraoperatively obtained peritoneal cytology in malignant ovarian tumors].

The evaluation of 259 peritoneal cytologic specimens obtained intraoperatively in cases of malignant ovarian tumor revealed positive findings in 62.5%; findings were doubtful in 2.3% and unusable in 2.7%. In the cases with clinically apparent peritoneal carcinomatosis, (n = 175), cytologic tests were positive in 75%. Taking tumor histology into account the high proportion of positive findings among the non-classifiable tumors (11 out of 13) was striking. For this reason, in a second investigation, 144 peritoneal cytologic specimens taken exclusively from cases of serous carcinomas were studied with regard to their degree of differentiation, the time of removal, and tumor spread. The peritoneal cytology was positive in 33% of the cases without peritoneal carcinomatosis at the first operation and in 36% of the cases with only histologically detectable tumor remnants at the time of the second-look operation. With one exception, peritoneal cytology was also negative in 26 second-look cases without clinically or histologically detected tumors. The incidence of positive peritoneal cytology findings was significantly higher in serous tumors with low-grade differentiation.

Ascitic Fluid↗
PubMed1986

The prognostic significance of the DNA distribution in squamous-cell carcinomas of the uterine cervix.

To establish the prognostic parameters of DNA distributions of squamous-cell carcinomas of the uterine cervix, cervical smears from 30 patients were examined cytomorphometrically ten years after the diagnosis had been made. Most of the patients had stage II cervical carcinomas that had been treated with primary combined irradiation. In the DNA histograms, the position of the stemline proved to be the prognostically significant factor. All of the patients with diploid or euploid-polyploid stemlines were still alive after ten years while only 17.6% of those with aneuploid stemlines have survived. No correlation between the ten-year survival rate and the proliferation rate of the tumors could be established.

Adult↗
PubMed1984

[Diagnostic value of increased lactate dehydrogenase activity in vaginal secretions for the early detection of diseases requiring treatment of the female genitals and breast].

The Diagnostic Validity of Enhanced Lactate Dehydrogenase Activity in the Vaginal Secretion for Early Diagnosis of Diseases of the Female Genitals and the Mamma in Need of Treatment. An impregnated hygiene tampon (Gynaegnost) was used, which by a colour reaction permits the determination of augmented activity of lactate dehydrogenase (LDH) in vaginal secretion. The diagnostic validity of the results of the findings by this method in the detection of diseases of the female genitals and the mamma needing treatment was examined in two clinical diagnostic studies. The first study including 486 patients chiefly suffering from manifest carcinoma was aimed at solving a deliberately limited question. In patients with carcinoma stages I to IV of the most important genital locations augmented LDH-activity (correct positive results) was found in about 90%. A second study comprised 1955 women entering into outpatient gynaecological services for preventive examination; the definition of this series was difficult. Three categories of diagnostic groups can be determined by the results of this study. In the first category with the most important subgroup "routine examination without specification of a pathological finding" less than 20% "false" positive test results by the indicator were observed. A second category with inflammatory diseases (colpitis, endometritis) showed positive results in about one-third of the examinations. The third category comprised a minor number of malignant tumors roughly with a percentage of positive results corresponding to that of the first study. Augmented LDH activity in vaginal secretion is a diagnostic criterion which detects not only cancer; it is similar to the test of occult blood in faeces.(ABSTRACT TRUNCATED AT 250 WORDS)

Breast Neoplasms↗
PubMed1984

Algorithm for a DNA-cytophotometric diagnosis and grading of malignancy.

An algorithm for processing data on nuclear DNA content obtained cytophotometrically was developed (1) to obtain an objective discrimination between benign and malignant lesions in conventional cytologic smears secondarily stained according to Feulgen and (2) to obtain an objective degree of tumor malignancy on a continuous scale of malignancy grades. Investigations in 258 malignant tumors (95 malignant lymphomas, 52 uterine cervix carcinomas, 28 prostate carcinomas, 18 breast carcinomas, 45 malignant bone tumors and 19 larynx carcinomas) and in 74 benign lesions in these organs yielded a diagnostic accuracy of no false-positive, no false-negative and 21% suspicious diagnoses. The probability that "suspicious" cases were malignant was 81%. The overall diagnostic accuracy for non-negative cases thus amounted to 100%. Results in 95 patients with different malignant lymphomas and in 16 patients with squamous-cell carcinoma of the larynx demonstrated the prognostic validity of the DNA-grading system.

Cell Nucleus↗
PubMed1982

[Demonstration of Chlamydia by means of vaginal cytology].

There are mostly metaplastic cells, which are affected by chlamydia, show a dense usually cyanophilic cytoplasm with vacuolization. They are characterized by more or less round granula, sometimes also amorphous inclusion bodies. The vacuoles are multiple and mostly perinuclear, sometimes the nucleus is pushed aside like a signet-ring cell. Also columnar cells of the endocervix may be infected by chlamydia. They are showing more less characteristic signs, mostly a fine vacuolated cytoplasm. A smear of the endocervix, wet fixed, stained by Papanicolaou, allows the suspected diagnosis "chlamydia-infection", if metaplastic or endocervical cells show these characteristic changes. The proof diagnosis however is only possible by cell-culture.

Bacteriological Techniques↗
PubMed1982

The new and reproducible Papanicolaou stain. Morphologic and spectrophotometric observations on the influence of stain composition on staining results.

The morphologic and spectroscopic characteristics of a new and reproducible modification of the Papanicolaou stain are briefly described. The main features of this modification are (1) replacement of the natural dye hematoxylin by the synthetic and chemically well defined dye thionin, (2) introduction of an alcoholic counterstain consisting of eosin gamma and fast green FCF only and (3) employment of alcoholic solutions only. The absorption characteristics of hematoxylin and thionin bound to chromatin are influenced by the cytoplasmic counterstaining, especially by the two green dyes, the absorption peaks of which are close to those of the nuclear stains. The implications of these results for visual and automated cytologic diagnosis are briefly discussed.

Cell Nucleus↗
PubMed1979

[The special case: squamous epithelial carcinoma of the cervix after cryotherapy in a 23 year old patient].

A case report is given of a 23 year old woman with an invasive squamous cancer of the uterine cervix 10 months after cryotherapy of an "ectopic area". The development in this case is reason for stressing the indispensable points in cryo-, thermo- and laser-therapy of the cervix: 1. proper pretherapeutic evaluation and documentation by means of cytology, colposcopy, and histology; 2. adequate extent of therapy; 3. exact follow-up after treatment.

Adult↗
PubMed1979

[Victimology of sexual crime. Examination of victims and the consequences for behavior, prophylaxis and therapy (author's transl)].

Medico-legal and criminological examinations of 93 sexual crimes are reported. This figure comprises all the crimes which have become known to the police during a certain period in one region. It is suggested that the medical examination by confined to specific wards of the gynecological hospital so that psychological care can be provided and the customary criminological investigation methods be changed. For preventive reasons, a general expertise of the offenders and credibility examinations of victims and offenders seem to be called for in the absence of satisfactory evidence. Physical resistance on the part of the victim in the present cases led to a significant increase in the injury and death rate. How far traditional social views toward women favor sexual crime is discussed.

Criminal Psychology↗
PubMed1979

[Simplification of the Papanicolaou stain which is easily reproducable (author's transl)].

A simple and well reproducable modification of the Papanicolaou stain is reported. The most important modification is the replacement of the natural stain hematoxyline by the qualitively excellent synthetic stain thionin which is immediately added to the alcoholic fixation solution for a fixation stain. The high labor intense alternation between alcohol water and alcohol is thus eliminated. The quality of the nuclear staining is very good. The fixation staining with thionin without counter-staining of the cytoplasma may be used for a rapid thionin stain which gives sufficient cytoplasmatic staining by the thionin for a rapid cytological smear for cancer. The counter-stain of the cytoplasma was also simplified. The over-all staining of this simplification of the pap smear is comparable to that of the original papanicolaou stain. This modification of the papanicolaou stain fulfills requirements which a stain in competition with the original papanicolaou stain should have. 1. The modification is qualitively equal to the original method, 2. the staining method is simpler, 3. the staining method is well reproducable, 4. the staining method is more economical.

Female↗
PubMed1978

[Early diagnosis of neoplasms in young women. When should prevention of neoplasms start?].

Cervical cancer is seen in younger women more often than expected. In the literature up to 35% of the pre-stages--especially of dysplasia form--have been found in the age group under 30 years. By taking cervical smears in all women experiencing sexual intercourse we would detect these cases and prevent invasive forms of cervical cancer. Therefore, we strongly recommend to lower the age limit of 30 in performing general Pap screening.

Adult↗
PubMed1978

The diagnosis of endometrial carcinoma by means of jet wash and DNA flow-through cytophotometry.

Flow-through cytophotometric determination of nuclear DNA content on jet wash material from the endometrium was employed in the diagnosis of endometrial carcinoma. One hundred and thirty cases were studied. In comparison to results from cytodiagnosis, flow-through photometry yielded a false negative rate of 31.6% and a false positive rate of 42.2%. The false negative findings resulted in part from the small relative frequency of atypical cells in a mixed population of normal and atypical cells in certain cases. Besides this, we often found carcinomas with a diploid DNA stem line, which could not be distinguished cytophotometrically from normal corpus endometrium (Sandritter, 1952; Atkin et al., 1959; Hustin, 1976). The flow-through photometrically false positive findings may have resulted either from cell aggregates or from a nuclear DNA content elevated over the diploid value in proliferating cells (D. Wagner et al., 1968; D. Wagner and Richard, 1968). The observed false negative and false positive rates demonstrate that flow-through photometric determination of nuclear DNA content is unsuitable for the diagnosis of endometrial carcinoma.

Biopsy↗