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Biomedical subjects

M Hashimoto

Publications and source records attributed to M Hashimoto.

At least 1,009 records · Page 56Linked to original sources
PubMed1986

Effect of caerulein with haloperidol on monoamine contents in rat brain regions treated with methamphetamine.

To elucidate the mechanism of the antagonistic effect of caerulein on amphetamine-induced hyperactivity, monoamine contents in rat brain regions were measured by HPLC. With a combination of caerulein and haloperidol, dopamine contents in methamphetamine treated-rats were significantly decreased in the striatum. Noradrenaline contents were significantly decreased in the haloperidol-methamphetamine group, but caerulein restored it to the normal levels. Serotonin contents did not change in any of the groups tested. These results may indicate that caerulein acts as a neuromodulator in rat brain and causes suppression of hyperactivity induced by methamphetamine.

Animals↗
PubMed1986

Electrophysiological effects of diphenylhydantoin in patients with sinus node dysfunction.

The electrophysiological effects of intravenous administration of diphenylhydantoin (DPH) (5 mg/Kg, maximum 250 mg) were studied in 20 patients with sinus node dysfunction (SND). DPH shortened spontaneous cycle length (SCL) in 3 patients and lengthened it in 3. Maximum corrected sinus node recovery time (max CSRT) was prolonged in 7 patients and shortened in 3. Estimated sinoatrial conduction time was prolonged in 3 patients and shortened in 2 of 10 patients in whom this measurement was possible. However, these changes were not statistically significant. Neither were there any significant changes in PA, AH and HV intervals nor refractory periods of the atrium, the AV node and the ventricle. DPH prolonged SCL and/or max CSRT in 9 of 20 patients with SND, and it was suggested that DPH has depressant effects on the sinus node in some patients with SND. Thus, this drug should be used with caution in the treatment of ventricular arrhythmias accompanied by sinus node dysfunction.

Adult↗
PubMed1986

Detection of type V collagen-degrading enzyme activity in human liver.

Type V collagen-degrading enzyme activity was detected as a metalloprotease acting at neutral pH in the human liver. Type V collagen extracted from human placenta and labeled with [1-14C] acetic anhydride was used as the substrate in the assay. Four major degradation products with relatively high molecular weights were observed upon polyacrylamide gel electrophoresis of the incubation mixture of type V collagen and liver homogenate. The significance of the measurement of this enzyme activity was discussed in relation to the clarification of the mechanism of liver fibrosis.

Collagen↗
PubMed1986

Assay procedures for cathepsin B, H and L activities in rat tissue homogenates.

Cathepsin B, H and L activities in small amounts of rat tissue homogenates corresponding to 10 micrograms protein were determined with 7-amino-4-methyl-coumarin conjugates as substrates. A new procedure for serum cathepsin H activity was also developed. High cathepsin B and H activities were found in kidney, spleen and liver. Liver cathepsin B, H and L activities in D-galactosamine-injured rats were decreased concomitantly with an increase in serum cathepsin H activity.

Animals↗
PubMed1986

A new antitumor complex, WF-1360, WF-1360A, B, C, D, E and F.

A complex of the new antitumor antibiotics (WF-1360, WF-1360A, B, C, D, E and F) was produced by Rhizopus sp. No. F-1360. Structural studies of these compounds suggested that they were novel 16-membered-ring lactones having an oxazole ring in their structures. WF-1360 was found to be identical with rhizoxin (1) and WF-1360B, C, E and F were determined to be homologues of 1 with structures 2, 3, 4 and 5, respectively. These compounds were cytotoxic when tested on P388 leukemia cells in vitro. WF-1360 was highly active against leukemia L1210 and melanoma B16. They also exhibited potent antifungal activities, but weak antimicrobial activities against some Gram-positive or negative bacteria.

Animals↗
PubMed1986

Elicited antibody nature of human monoclonal protein with anti-streptolysin O activity--analysis with monoclonal anti-idiotype antibody.

Sera from 7 patients with multiple myeloma having antistreptolysin O (ASO) activity in high titers were detected by a streptolysin O (SLO) inhibition assay. However, activity was in low titer when assayed by a passive agglutination assay. The discrepancy between these 2 assays raised some doubts as to whether these monoclonal proteins (M.protein) bond to SLO in the same manner as elicited antibodies. Immunochemical analysis and idiotope analysis using monoclonal antibody to one of these M.proteins strongly suggest that M.protein with ASO activity bind to SLO in a manner similar to elicited antibody. The discrepancy between the 2 assays might be due to differences in the antigenic structure of different forms of the SLO molecule.

Antibodies↗
PubMed1986

[Continuous measurement of tumor blood flow under hypertension induced by angiotensin II--clinical studies with laser Doppler velocimetry].

The effect of treatment with intravenously administered Angiotensin II (AT II) on blood flow in normal and malignant tissues was investigated clinically. The time course of the effect of AT II was directly recorded by laser doppler velocimetry (LDV) via a probe placed on the surface of normal and malignant tissues. Intravenous administration of AT II resulted in an approximate 3.5 (1.3-14.0)-fold increase in blood flow in eleven malignant tissues, such as breast cancer with direct extension to the skin and abdominal skin metastasis of gastric adenocarcinoma. On the other hand, the blood flow in normal skin was decreased under AT II-induced hypertension, but a reactive hyperemia-like increase was observed soon after the withdrawal of AT II. These results strongly suggested that intravenously administered AT II can act as an adjuvant to enhance, by varying degrees, drug delivery to tumor tissue in cancer chemotherapy and that the administration of chemotherapeutic agents is undesirable soon after the withdrawal of AT II.

Adenocarcinoma↗
PubMed1986

Tissue levels, tissue angiotensin converting enzyme inhibition and antihypertensive effect of the novel antihypertensive agent alacepril in renal hypertensive rats.

Tissue levels, tissue angiotensin I converting enzyme (ACE) inhibition and hypotension were examined 20 min, 1, 5 and 14 h after oral administration of 1-[(S)-3-acetylthio-2-methylpropanoyl]-L-prolyl-L-phenylalanine (alacepril, DU-1219) (37.5 mg (92 mumol)/kg) or 1-[(S)-3-mercapto-2-methylpropanoyl]-L-proline (captopril) (20.0 mg (92 mumol)/kg) in renal hypertensive rats, using 14C-labeled compounds. Alacepril exerted a more gradual and more sustained antihypertensive effect than captopril. The maximal hypotension was observed 1 and 5 h after administration of captopril and alacepril, respectively. After administration of [14C]captopril, serum level reached the maximum at 20 min and then decreased rapidly. After administration of [14C]alacepril, serum level reached the maximum at 1 h and decreased more slowly than after [14C]captopril. Time course patterns of tissue levels were essentially in parallel with those of serum levels. Captopril exerted the maximal reduction of ACE activity in tissues 20 min after oral administration and thereafter, the reduction was diminished with time rapidly. [14C]Alacepril showed gradual reduction (the maximum at 1 h) and recovery of ACE activity relative to captopril. After oral administration of [14C]alacepril, tissue unbound fractions contained captopril and its derived metabolites while serum unbound fraction contained the intermediate metabolite desacetyl-alacepril (DU-1227) as well. Correlations between ACE inhibition and tissue levels and between changes in tissue ACE inhibition and in blood pressure with time after oral administration of the two agents were discussed. Furthermore, the direct comparison of alacepril and captopril was attempted by the difference in blood pressures and in ACE inhibitions induced after oral administration of the agents.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1986

Metabolism of protein conjugate of desacetyl-alacepril and its effect on angiotensin converting enzyme in renal hypertensive rats.

The fate of protein conjugate of desacetyl-alacepril (DU-1227) and its effect on angiotensin I converting enzyme (ACE) activity in renal hypertensive rats were studied. [14C]DU-1227-protein conjugate was prepared by ultrafiltration method and administered intravenously in rats. Elimination of radioactivity of [14C]DU-1227-protein from plasma after injection seemed much slower than that reported of [14C]alacepril (1-[(S)-3-acetylthio-2-methylpropanoyl]-L-prolyl-L-phenylalanine, DU-1219) given orally. In the plasma unbound fraction, captopril and captopril-cysteine were detected. Most tissue levels were higher than plasma levels. Significant reduction of tissue ACE activity was seen after administration of the conjugate. Radioactivity was mostly excreted in feces. Captopril, captopril disulfide and captopril-cysteine were found as urinary metabolites. These findings indicate that protein-bound DU-1227 readily dissociated and released DU-1227 was converted to captopril in vivo and can therefore participate in prolonged hypotensive effect exerted by alacepril.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1986

Low incidence of post-transfusion hepatitis in patients with liver cirrhosis.

A study has been undertaken to determine the incidence of post-transfusion hepatitis in 57 patients with liver cirrhosis (non-B type) and 93 cases without liver disease, who received blood transfusion during major surgery. A significantly lower incidence of post-transfusion hepatitis (5.3%) was found in patients with liver cirrhosis than in those (17.2%) without liver disease. Two out of three cirrhotic patients with post-transfusion hepatitis progressed to the clinical state of hepatic failure.

Adolescent↗
PubMed1986

[Echo and Doppler cardiographic findings of isolated quadricuspid aortic valve: a case report and a review of the literature].

The two-dimensional and pulsed Doppler echocardiographic features of a case of isolated quadricuspid aortic valve with aortic regurgitation are described. A 62-year-old woman was hospitalized for exertional palpitation and dyspnea. Her physical examination showed the typical findings of aortic regurgitation. Two-dimensional echocardiograms revealed the aortic valve to have four cusps of nearly equal size. The accessory cusp was situated between the right and left coronary cusps. By pulsed Doppler echocardiography, holodiastolic turbulent flow signals were observed in the left ventricle, and the aortic flow pattern showed holodiastolic reverse flow, indicating severe aortic regurgitation. These findings were confirmed by aortography and by surgery. The coronary arteries and pulmonary valve were normal. We attempted a classification of the anatomical variations of the previously reported 34 cases of isolated quadricuspid aortic valves, including our own. They were classified as one smaller (67%), four equal (18%), three smaller (6%), four unequal (6%) and two smaller (3%) types. Twenty of the 34 patients had aortic regurgitation. Bacterial endocarditis and congestive heart failure were the main causes of death.

Aortic Valve↗
PubMed1986

[Analysis of mitral inflow velocity pattern in relation to left ventricular end-diastolic pressure].

The relationship between mitral inflow velocity patterns and left ventricular end-diastolic pressure (LVEDP) was evaluated using pulsed Doppler echocardiography in 34 cases of heart disease, without significant valvular regurgitation. Flow patterns in 19 of the 34 cases were also examined before and after the elevation of LVEDP by methoxamine infusion, 0.01 mg/kg/min. The ratio of the peak velocities in the atrial contraction phase to that in the rapid filling phase (A/R) and the ratio of mean acceleration rates to peak velocities in the rapid filling phase (ACR/R) were determined from the mitral flow patterns obtained by the apical approach. 1. ACR/R correlated significantly with LVEDP (r = 0.49), but A/R did not. LVEDP in six cases with normal A/R (0.5 to 1.0) was 8.3 +/- 2.9 mmHg (mean +/- SD). Among 19 cases with A/R of 1.0 or more and ACR/R less than 13 sec-1, LVEDP showed 10.2 +/- 3.8 mmHg. In eight cases with A/R of 1.0 or more and ACR/R of 13 sec-1 or more, LVEDP was 17.9 +/- 6.2 mmHg. The average value of LVEDP in two cases with A/R less than 0.5 was 18.5 mmHg. 2. When the LVEDP was elevated after methoxamine infusion, A/R within normal range increased in five of six cases and decreased in the remaining case. A/R more than 1.0 decreased in 10 of 11 cases and ACR/R tended to increase with increasing LVEDP.(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗