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Biomedical subjects

M Harada

Publications and source records attributed to M Harada.

At least 1,189 records · Page 66Linked to original sources
PubMed1983

[Ultrastructural changes of the tegument on Diphyllobothrium erinacei and Hymenolepis nana treated with paromomycin sulfate in vitro].

The effects of paromomycin sulfate on Diphyllobothrium erinacei and Hymenolepis nana in vitro were examined morphologically with a scanning and a transmission electron microscope. D. erinacei was incubated for 3 and 6 hours at 37 degrees C in a culture medium, 0.85% physiological saline solution, containing 0.5% paromomycin sulfate. H. nana was incubated in the same medium for 3 hours only. The concentration of paromomycin sulfate was set basing on the results which Kitamoto (1968) reported as the concentration level in feces after administrations of this drug in a clinical survey. The effect of the drug on the surface structure in both worms appeared markedly in the neck region. Mechanisms of breakdown on the tegument were supposed as follows. First, microtriches were disconnected from the tegumental surface and many vesicles were formed in the cytoplasm of the tegument. Finally, the tegument layer was excoriated to exposed the basal lamina. In 6 hours incubation, this surface of the worm suffered more damage than that in 3 hours. The damage of the basal lamina as in the case of D. latum expelled from a man by paromomycin (Y. Tongu et al.), however, could not be observed in the present study in vitro. It suggests that the destruction of basal lamina usually observed with the expelled worms from clinically treated human might be due to the combined effect of digestive enzymes secreted from host and the mechanical impact of intestinal peristalsis. Some of the vesicles in the tegument may originate from mitochondria because the fine structure of cristae were occasionally observed remaining in the vesicles.

Animals↗
PubMed1983

[Prognostic factors and surgical method in patients with leiomyosarcoma of the stomach--with special reference to local tumor excision].

Between 1962 and 1982, 48 patients with leiomyosarcoma of the stomach who had undergone resection at the National Cancer Center Hospital were studied to define the prognostic factors clinicopathologically and the criteria of local excision of the tumors. The 5 and 10-year survival rate after resection was 84.3% and 80.5% respectively. Since the extent of surgical resection did not affect survival, gastrectomy is of no greater benefit than local excision. The important prognostic factors were location, size, mitotic index and cellularity of the tumor. Criteria of local tumor excision are defined as tumor location in the anterior wall, small size (much less than 5.0 cm in diameter), low mitotic index (5.0/25 HPF) of low cellularity (0-3/0.0004 mm2).

Adult↗
PubMed1982

Mass screening for urinary tract cancer with urine cytology.

A rapid and simple cytologic screening technique for fresh urine specimens stained with toluidine blue is described. As to the validity and description of cell structure, the accuracy of urine cytology with this procedure is very similar to that of the Papanicolaou technique. Utilizing this technique, a hospital population was screened for urinary tract cancer from specimens sent for routine urinalysis. In this study of 16,062 fresh urine sediments selected at random from nonurologic patients, a total of three cases of histologically proven bladder cancer were detected, corresponding to a rate of one bladder cancer in 5,000 individuals. The results emphasize the usefulness of this simple screening technique in combination with clinical urinalysis and cytology for early detection of urinary tract malignancy.

Female↗
PubMed1982

Purification and properties of bovine dental-pulp alkaline-phosphatase.

Alkaline phosphatase (E.C.3.1.3.1.) from unerupted bovine pulp was extracted from the microsomal fraction with eta-butanol and purified 77-fold, using DEAE-cellulose chromatography, Sephadex G-200 gel-filtration and concanavalin-A affinity chromatography, to a final specific activity of 92.3 units/mg protein. Affinity chromatography confirmed the glycoprotein nature of the enzyme. The pH optimum for the purified enzyme was 10.0 with rho-nitrophenylphosphate, and 8.7 with phosphoserine. The apparent Km was estimated to be 0.7 mM, using rho-nitrophenylphosphate in glycine-NaOH buffer, pH 10.0. The enzyme was markedly inhibited by EDTA, bromotetramisole and homoarginine but was insensitive to phenylalanine, and therefore resembled the alkaline phosphatase of liver and bone, but not that of intestine and placenta. No protein phosphatase activity towards dentine phosphoprotein and phosvitin was observed.

Alkaline Phosphatase↗
PubMed1982

Serum dipeptidyl peptidase (DPP) IV activities in oral cancer patients.

Dipeptidyl peptidase (DPP) IV activities in normal human sera and in the sera of patients with malignant tumors of the oral region were assayed. Patients with oral squamous cell carcinoma had significantly lower enzyme activity than that of normal controls (p less than 0.001). Patients with advanced carcinoma showed a more significant reduction of serum enzyme activity. On the other hand, in oral squamous cell carcinoma patients with hepatic dysfunction, the serum enzyme activity was significantly increased (p less than 0.02).

Adult↗