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Biomedical subjects

M Goudemand

Publications and source records attributed to M Goudemand.

At least 127 records · Page 7Linked to original sources
PubMed1977

[Detection and titration of antitetanus antibodies using an automated passive hemagglutination method].

An automated reversed passive haemagglutination technique is described, using human erythrocytes sensitized by the chromic chloride method with higly purified tetanus toxoid. Such erythrocytes are agglutinated by the specific antibodies in a Technicon autoanalyzer. Clots are decanted, supernatant is hemolyzed and optical density is measured at 550 millimicron. Assay standards for comparison, ranging from 5 to 25 UI/ml, are prepared from human antitetanus immunoglobulins titrated by toxin neutralisation assay in mice. This method allows to screen donors' sera for presence of high titers of tetanus antibodies (larger than or equal to 5 UI) suitable for preparation of antitetanus immunoglobulins. 4,4% of donors have circulating antitetanus antibody levels corresponding to 5 UI/ml or more, by this method. The specificity of antibodies has been confirmed by the neutralisation assay in mice. The results obtained among 1.000 donors well agree with those of the counter-immuno-electrophoresis technique (C.E.P.). But, when, compared with C.E.P., the haemagglutination assay appears more objective, more quantitative and sensitive, and allows to get not only a rapid screening test but also a precise titration simultaneously.

Antibodies, Bacterial↗
PubMed1977

[HLA antigen and Willebrand's disease (author's transl)].

The frequency of HLA antigens was determined in 28 unrelated patients with Willebrand's disease and in 326 healthy controls. There was no significant difference between the two groups for any of the HLA antigens tested. Segregation analysis processed among the families of 26 of these patients didn't allow us to find any disorder of the HLA antigens distribution in the patient's group: transmission of the disease and transmission of the HLA haplotypes appear to be independant in 25 of 26 families studied. There is no linkage and no association between the Willebrand's disease genes and the HLA system genes.

HLA Antigens↗
PubMed1977

[Enzyme-linked immunoabsorbent assay of factor VIII-related antigen. Interest in study of Von Willerbrand's disease (author's transl)].

Factor VIII-related antigen is measured by ELISA as follows: Factor VIII-R: Ag is first "captured" between rabbit antibodies to human factor VII R : Ag adsorbed in polystyrene tubes, and conjugate (peroxydase-labelled rabbit antibodies). A chromogenic enzyme substrate is then added and absorbence measurement allows subsequent assay. This method is sensitive, reproducible and specific. The comparison between the results obtained by this enzyme immuno-assay and the electro-immunodiffusion assay (EIA) is interesting for the study of quantitative and qualitative variations of F VII R : Ag in von Willebrand's disease. Two observations of von Willerbrand variants are described in detail : VIII R : Ag plasma level of the first one is normal using EIA but decreased using ELISA, its electrophoretic mobility is increased; in the second case we found typical anomalies of von Willebrand except the VIII R : Ag level normal by the two methods.

Antigens↗
PubMed1977

[Laparotomy in the therapy of Hodgkin's disease].

We have performed laparotomy and splenectomy for seventy patients with Hodgkin's disease. Among 43 stages I or II before exploration, primary laparotomy revealed ignored abdominal localizations in 16 cases (37,2%). Involvement of the spleen occurred in 13 of these 16 patients, lonely (5 cases), associated with positive nodes (4 cases) or associated with positive nodes and liver involvement (4 cases). Among 4 stages III before exploration, no regression was found. Among 9 patients with presumed stages I or II above the diaphragm and previously treated by radiotherapy, delayed laparotomy revealed abdominal relapse in 6 cases. At last, among 14 patients with obvious stages II or IV and previously treated by combination chemotherapy, laparotomy, caused by persistent evolutive signs, showed residual localizations under the diaphragm in 8 cases. According to these results, we preconise immediate laparotomy in almost all presumed stages I or II above the diaphragm. About third of these subjects have their staging advanced to stage III or IV and, according to us must be treated by combination chemotherapy while the remaining cases justify of radiotherapy. In obvious stages III and IV delayed laparotomy may be useful in some circumstances, after chemotherapy.

Cobalt Radioisotopes↗
PubMed1976

Hereditary hemolytic anemia with erythrocyte phosphofructokinase deficiency: studies of some properties of erythrocyte and muscle enzyme.

A case of hereditary nonspherocytic hemolytic anemia associated with partial erythrocyte PFK deficiency without muscular symptoms is reported: erythrocyte enzyme activity in the propositus was 60% of normal. Kinetic studies of erythrocyte PFK revealed increased sensitivity to ATP inhibition and decreased sensitivity to citrate inhibition. Muscle PFK from the patient had a normal enzymatic activity, but was highly unstable to heat, dilution without stabilizer and urea; furthermore its starch gel electrophoretic mobility was markedly faster than the one of a normal control. The results suggested that a muscle type's subunit was deficient in the erythrocyte PFK. The authors hypothesize that there was no PFK deficiency in the patient's muscle because of the active synthesis of proteins by this tissue. In contrast, the deficiency of PFK would be easily detected in erythrocytes, because of the absence of protein synthesis.

Adenosine Triphosphate↗
PubMed1975

[Cardiac insufficiency caused by the use of daunorubicin. Clinical and developmental study of 4 recent cases].

Out of a total of 126 patients with acute lymphoblastic leukemia, including 69 children aged less than 15 years, and 57 adults aged less than 60 years, who were treated with daunomycin (D.N.R.) initially and with several courses of reinduction, we noted 4 cases of decompensated heart failure (3.1%). 3 cases occurred in children aged 4, 5 and 5 years, and in the last case, in a young adult, of 19 years. Three of these patients were in complete remission of the blood and were only undergoing courses of reinduction. The 4th was in incomplete remission. When symptoms first occurred, about 2 months after the last injection of D.N.R. the total dose administered varied between 14 and 31 mg/kg, extending over periods of 5 to 16 months (average monthly dose 1.9 to 2.8 mg/kg). Except in one case, the course only mildly influenced by the symptomatic treatment and death occurred in 3 patients within from 15 days to 5 months after the onset of the first symptoms. The last patient is still alive and seems stabilised with a follow up period of 9 months. The responsibility of D.N.R. in the development of this myocarditis appears very likely. The unforeseable character of such complications, which are independent of age, and not necessarily linked to any excessive dosage, should be taken into consideration in the indications for D.N.R. in spite of their relative rarity.

Adult↗