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Biomedical subjects

M Ghosh

Publications and source records attributed to M Ghosh.

At least 145 records · Page 8Linked to original sources
PubMed1986

Recurrent corneal erosion, microcystic epithelial dystrophy, map configurations and fingerprint lines in the cornea.

A 45-year-old lady presented with a recurrent corneal erosion. On closer examination, she was found to have epithelial microcysts and fingerprint dystrophy. Pathologic material from the affected cornea showed microcysts, protrusions of basement membrane and evidence of poor adhesion between the basement membrane and the epithelial cells, as well as between the epithelial cells themselves. The case demonstrated the clinical and pathologic features of recurrent corneal erosion, microcystic disease and fingerprint dystrophy of the cornea, together with the pathologic findings of map dystrophy. The authors suggest that these four diseases are various expressions of a clinicopathologic entity, epithelial basement membrane corneal dystrophy. A pathologic explanation for recurrent corneal erosion is apparent.

Cornea↗
PubMed1985

Production of a Lectin in Tissue Cultures of Dolichos biflorus.

Callus cultures have been produced from the epicotyl and leaves, hypocotyl, and roots of germinating Dolichos biflorus seeds. These cultures were initiated on media containing 2,4-dichlorophenoxyacetic acid and kinetin, transferred to media with increased amounts of these hormones, and then maintained on hormone-free media. Extracts of these cultures were examined by radioimmunoassays specific for the lectin from the seeds of this plant and for a lectin that is present only in the stems and leaves of the intact plant. Although the seed lectin was not detected in any cultures, the stem and leaf lectin was produced in those cultures grown on the hormone free media. Lectin isolated from these cultures had subunits identical in electrophoretic mobilities to the subunits from the lectin isolated from intact stems and leaves. Levels of this lectin decreased when the cells were transferred back to media containing hormones and increased again upon transfer to the hormone-free media. The absence of exogenous hormones and the production of lectin were also correlated with the rapid growth and greening of the cells. Immunofluorescence and immunocytochemical studies on sections of cultured cells indicated that the stem and leaf lectin is associated with the cytoplasm as well as the cell wall as has been found in previous studies on the subcellular localization of this lectin in the intact plant.

Journal Article↗
PubMed1985

Corneal changes in Fabry's disease: a clinico-pathologic case report of a heterozygote.

Fabry's disease is an X-linked recessive inborn error of metabolism, caused by a deficiency of alpha galactosidase A. This report describes a heterozygote patient with multiple system problems diagnosed eventually as Fabry's disease by the ocular findings. The clinical diagnosis was confirmed by enzymatic assay. Our report emphasizes: The variability of the non-ocular manifestations in the heterozygote of Fabry's disease. The diagnosis of Fabry's disease in our patient was made by the ophthalmologist. The ultra-structural changes in the cornea and conjunctiva of the heterozygote confirm those reported in the literature; in addition we describe changes in the goblet cells. The clinical and ultra-structural similarities of the deposits in Fabry's disease, Chloroquin keratopathy and Amiodarone keratopathy are striking and will be discussed.

Adult↗
PubMed1984

Ultrastructural changes in the cornea and conjunctiva of a heterozygous woman with Fabry's disease.

Heterozygous females with Fabry's disease show a typical whorled pattern in the corneal epithelium. The patient presented in this paper also had symptoms of polymyositis, necrotizing vasculitis and angiokeratomas. The diagnosis was made from the corneal changes. Electron microscopy revealed intracytoplasmic electron-dense deposits made up of lamellar stacks of uniform periodicity similar to myelin. These were present not only in the corneal epithelium but also in the epithelium, goblet cells, endothelium and mural cells of blood vessels in the conjunctiva. The material in the goblet cells may account for the known presence of ceramide trihexoside in the tears.

Adult↗
PubMed1984

Amiodarone-induced ultrastructural changes in human eyes.

Ocular observations in a series of 100 patients treated with amiodarone, along with the pathological changes observed in the eye tissues of two patients treated with this drug, are described in this paper. In the latter two patients intracytoplasmic membrane-bound lamellar bodies similar to myelin were observed by electron microscopy not only in the corneal epithelium and fibroblasts, the conjunctiva and the lens, but also in the corneal endothelium, the iris, the ciliary body, the choroid and the retina. It is suggested that patients taking amiodarone in high dosage for long periods have their eyes and retinal function monitored.

Amiodarone↗
PubMed1983

Homogeneous enzyme immunoassay of estriol in picogram amounts.

A homogeneous enzyme immunoassay for estriol has been described using estriol-glucose-6-phosphate dehydrogenase (E3G6PD) as enzyme conjugate. Antisera for estriol were raised by immunising rabbits with two different immunogens, estriol-6-(O-carboxymethyl oxime) bovine serum albumin (E36CMOBSA) and estriol-3-carboxymethyl ether bovine serum albumin (E33CMEBSA). A new method for preparation of estriol 3-O-carboxymethyl ether in high yield (90%) has also been described. Addition of anti-estriol antibodies to E3-G6PD conjugate resulted in inhibition of enzyme activity. In the presence of free estriol, the antibody induced inhibition of enzyme activity was reduced in a concentration dependent manner. The use of the heterologous combination between immunogen and enzyme conjugate, i.e. using anti-E33CMEBSA and E36CMOG6PD improves the sensitivity at the expense of specificity, the cross-reaction with other estrogenic hormone being 10-15%.

Antibody Specificity↗
PubMed1983

A simple and rapid method for quantification of hapten specific antibodies.

A sensitive and simple method for quantitation of antibodies against small molecules is described using DNP-lysozyme as the enzyme conjugate. The anti-DNP antiserum was raised against DNP-bovine serum albumin conjugate. Anti-DNP antibody or its monovalent fragment (Fab) reduced the enzyme activity of DNP-lysozyme conjugate in a concentration-dependent manner. The inhibition of enzyme activity is a specific measure of the antibody and Fab content of the sample. The specificity of the reaction was assessed by reduction of antibody-induced inhibition by DNP-lysine. The ability of DNP-lysine to reduce the antibody-induced inhibition of DNP-lysozyme activity also makes possible a sensitive assay for DNP-lysine.

Animals↗
PubMed1983

Inhibition of ovarian aromatase by prolactin in vivo.

Ovarian aromatase activity was inhibited by prolactin treatment in both cycling rats and immature gonadotropin-primed animals. Cycling rats were injected s.c. with prolactin (4 mg/kg BW) beginning on diestrus 1. Aromatase activity in the largest follicles on diestrus 2 and on proestrus was reduced relative to controls. None of the prolactin-treated animals had ovulated when examined on the expected estrus morning. In other experiments, the immature pregnant mare's serum gonadotropin (PMSG)-primed rat was used as a model for the cycling rat. Microsomal aromatase activity but not the activity of the C17-21 lyase was reduced in ovaries of animals injected with prolactin [6 IU (200 micrograms)/rat] 48 h after PMSG administration and sacrificed 4 h later. Furthermore, aromatase activity was significantly increased when endogenous prolactin was inhibited by bromocryptine (CB-154). The effect of LH treatment, on the other hand, was to increase both aromatase and lyase activities. However, prolactin pretreatment did not consistently prevent the LH-induced increase in aromatase activity. The results suggest that inhibition of ovulation by prolactin is mediated, at least in part, by direct inhibition of aromatase activity.

Animals↗
PubMed1983

Corneal and conjunctival deposits in monoclonal gammopathy.

An IgG kappa monoclonal gammaglobulinopathy was discovered during investigation of long-standing corneal deposits. The deposits were located between the basal epithelial cells, in the anterior layer of Bowman's membrane and in keratocytes. This is the third reported case of corneal deposits associated with Bence Jones proteinuria and the fifth case of corneal deposits associated with crystalline inclusions within plasma cells of the bone marrow. A biopsy of the buccal mucosa showed intraepithelial inclusions. Immunoelectrophoresis of the tears revealed a small amount of secretory IgA and a moderate amount of IgG, practically all kappa light chains.

Conjunctiva↗
PubMed1983

The origin of senile lens exfoliation.

The uninvolved lens was removed from a patient with unilateral lens exfoliation, and electron microscopic examination of the lens confirmed the absence of exfoliation. Five years later white granules appeared on the vitreous face, and when the eye was removed at autopsy a further 4 years later typical exfoliation fibres were found in close association with the epithelium of the iris and the ciliary body and in clumps in the vitreous.

Aged↗
PubMed1982

Serum estradiol 17 beta, progesterone, and relative luteinizing hormone levels in Saimiri sciureus: cyclic variations and the effect of laparoscopy and follicular aspiration.

With increasing use of the squirrel monkey (Saimiri sciureus) in reproductive studies, a precise knowledge of the hormonal variations within the cycle becomes essential for timing of ovulation. The frequent utilization of laparoscopy for follicle aspiration and oocyte recovery warrants analysis of hormonal alterations. In the first study, estradiol 17 beta, progesterone, and luteinizing hormone (LH) levels were quantitated daily in the same group of 14 animals for 16 days. Serum estradiol and LH levels showed significant increases on day 4 of the cycle. Progesterone levels peaked on day 6. Based upon the hormonal peak intervals, the reproductive cycle of the squirrel monkey was estimated to be 8-9 days in length. Laparoscopy did not alter normal serum levels of estradiol, progesterone, and LH. Aspiration of the follicular contents, however, resulted in a significant decrease in estradiol and LH, and a rise in progesterone within 2 h.

Animals↗
PubMed1982

Amiodarone-induced cornea verticillata.

Among 37 patients treated with amiodarone, an antiarrhythmic drug, a typical keratopathy developed in 35, none of whom had ocular complaints. The keratopathy resembled that seen with Fabry's disease and chloroquine use, as did the membrane-bound lamellar bodies detected by electron microscopy in all layers of the corneal epithelium in the one patient with marked keratopathy in whom a corneal biopsy was performed; membrane-bound bodies, mostly granular, were also noted within this patient's stromal keratocytes. The possible pathogenesis of the keratopathy as a type of drug-induced lipidosis is discussed.

Adult↗