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Biomedical subjects

M Fukuda

Publications and source records attributed to M Fukuda.

At least 1,369 records · Page 76Linked to original sources
PubMed1980

K562 human leukaemic cells express fetal type (i) antigen on different glycoproteins from circulating erythrocytes.

During the ontogenic change from fetal to adult human erythrocytes, as well as fetal haemoglobin being replaced by adult haemoglobin, the cell-surface antigen i is converted to I (ref. 1). Recently it has been shown that this antigenic change is the conversion of the linear repeating Gal beta 1 leads to 4GlcNac beta 1 leads to 3Gal structure to branched Gal beta 1 leads to 4GlcNac beta 1 leads to 3(Gal beta 1 leads to 4GlcNac beta 1 leads to 6)Gal structure. We have shown that cell-surface labelling followed by endo-beta-galactosidase digestion can distinguish these two forms on the cell surface, and that band 3 and band 4.5 are the major carriers for these antigens on mature erythrocytes. Human leukaemic cell line K562, originally isolated from a patient at blast crisis of chronic myelocytic leukaemia, has recently been shown to synthesize glycophorin A, and to be capable of synthesizing haemoglobin upon induction. I demonstrate here that K562 cells express the fetal type (i) antigen on distinctly different glycoproteins from those of erythrocytes, by the use of cell-surface labelling followed by endo-beta-galactosidase digestion or followed by immunoprecipitation with specific antibodies.

Antigens, Neoplasm↗
PubMed1980

Detection of laser--UV microirradiation-induced DNA photolesions by immunofluorescent staining.

A low-power laser-UV microbeam of wavelength 257 nm was used for microirradiation of a small part of the nucleus of Chinese hamster cells. Following fixation in interphase or in the subsequent metaphase indirect immunofluorescent staining was performed with antiserum to photoproducts of DNA treated with far UV light. The results show that antibodies specific for UV-irradiated DNA can be used for a direct detection of laser-UV microirradiation-induced DNA photolesions. The potential usefulness of this method for investigation of the spatial arrangement of chromosomes in the interphase nucleus is discussed.

Animals↗
PubMed1980

Fluorescence fading and stabilization in cytofluorometry.

The factors affecting fluorescence fading in cytofluorometry were investigated using different kinds of nuclear staining, mounting media, and procedure of specimen preparation. Acceleration of fluorescence fading was observed in smear specimens treated with RNase, trypsin, or hypotonic solution before pararosaniline Feulgen nuclear staining. Similar effect was found for other DNA-stainings such as "33258 Hoechst" and Feulgen reactions with different Schiff-type dyes, such as acriflavine-SO2 and cresyl-violet-SO2, when chemically pure DNA was used. Fluorescence decay was rapid for all fluorochromes examined, when glycerin or buffer solution was used as mounting medium. Marked stabilization of fluorescence emission was induced in specimen mounted in non-fluorescent resin, Entellan (Merck), after post-staining fixation with absolute methanol for all tested fluorochromes. The same treatment induced almost complete fluorescence stabilization of fluorescein isothiocyanate (FITC); no detectable fluorescence fading was observed in a specimen stained with indirect immunofluorescence reaction using anti-UV-DNA antibody, during storage for 2 years at room temperature without special protection against light. These observations suggest that factors which bring about conformational stability of macromolecule-dye complexes generally induce fluorescence stabilization.

Animals↗
PubMed1980

Nonspecific ("pseudo-plasmal") dye-binding in the Feulgen nuclear stain and its blocking by azocarmin G.

In Feulgen nuclear staining nonspecific dye-binding due to the "pseudo-plasmal reaction" is intensified in isolated cells with intact cytoplasm, and cannot be eliminated by the post-irradiation method. Fluorescence intensity in the cytoplasm sometimes exceeds that of specific nuclear fluorescence, especially in brain and heart muscle cells, and it was almost impossible to perform cytofluorometric DNA quantification on such specimens. Various kinds of aldehyde-blocking agents such as sodium borohydride, 2,4-dinitrophenylhydrazine, aniline, and sodium pyrosulfite were effective in reducing the "pseudo-plasmal reaction". But the blocking effects were not complete because of additional release of reactive aldehyde groups during subsequent Feulgen hydrolysis. Acidic azocarmin G produced a complete block of all "pseudo-plasmal reaction" in acriflavine-Feulgen nuclear staining, allowing accurate DNA-cytofluorometry to be carried out.

Acriflavine↗
PubMed1980

Inhibitory effect of progesterone on follicular growth and induced superovulation in the rat.

We investigated the effect of exogenous progesterone on follicular growth and pregnant mares' serum gonadotropin and human chorionic gonadotropin (PMS-HCG) induced superovulation in intact and hypophysectomized immature rats. Although the administration of progesterone during PMS-HCG injections had no effect on superovulation, the administration of progesterone beginning 3 days before PMS injection inhibited superovulation in both intact and hypophysectomized rats. In order to study the mechanism of inhibition of superovulation by pretreatment with progesterone, an autoradiographic study using tritiated thymidine was done in hypophysectomized rats just before PMS injection. The labelling indices of granulosa cells were significantly decreased by the administration of progesterone (p < 0.001). Treatment with progesterone resulted in an increase of the follicles in early stages of atresia with pycnotic nuclei in granulosa cells surrounding apparently normal oocytes. Ovarian histology and serum and ovarian tissue concentrations of estradiol were then examined in hypophysectomized rats 20 h after HCG injection. Large Graafian follicles and corpora lutea were rarely seen in rats previously given progesterone, whereas many large Graafian follicles and corpora lutea were observed in the control rats. The concentrations of estradiol in the progesterone-pretreated rats were significantly reduced in serum (p < 0.02) and slightly reduced in ovarian tissues when compared with controls.

Animals↗
PubMed1980

Membrane differentiation in human erythroid cells: unique profiles of cell surface glycoproteins expressed in erythroblasts in vitro from three ontogenic stages.

HUMAN ERYTHROBLASTS IN CULTURE, IRRESPECTIVE OF THE ONTOGENIC STAGE OF THEIR PROGENITORS, ARE CHARACTERIZED BY: (i) the barely detectable amount of band 3 glycoprotein, (ii) the presence of two glycoproteins with molecular weights 105,000 and 95,000, (iii) the high concentration of glycophorin, and (iv) a minimum quantity of the carbohydrate chain susceptible to endo-beta-galactosidase ("polylactosaminoglycan"). In contrast, mature erythrocytes, whether of fetal, neonatal, or adult origin, are characterized by a high concentration of band 3 glycoprotein, polylactosaminoglycan, and glycophorins, but do not contain 105- and 95-kilodalton-glycoproteins. Thus, the process of erythroid maturation from erythroblasts to erythrocytes is accompanied by the appearance of band 3, the disappearance of 105- and 95-kilodalton glycoproteins, and a great increase in the quantity of polylactosaminoglycan. The structure of polylactosaminoglycan may not be different between mature erythrocytes and erythroblasts from the same ontogenic stage, but it is distinctively different from one stage to the other. The profiles of oligosaccharides released by endo-beta-galactosidase and immunofluorescence studies with anti-Ii antibodies indicated that a linear polylactosaminoglycan structure was present in erythroblasts as well as in erythrocytes of the fetal and newborn stage, whereas a branched polylactosaminoglycan structure was present in erythroblasts as well as erythrocytes of adult blood. Thus, two membrane characteristics are closely associated with the process of erythroid cell development; one-the membrane proteins band 3, band 4.5, and 95- and 105-kilodalton glycoproteins-determines the degree of maturation, and the other-polylactosaminoglycan-may determine the ontogenic stage of the erythroblast progenitors.

Anion Exchange Protein 1, Erythrocyte↗
PubMed1980

The effect of chloramphenicol and thiamphenicol on the ultrastructure of the erythroblasts in mouse splenic colonies.

Lethally irradiated mice were injected with 1 x 10(5) normal bone marrow cells taken from mice of the same strain. They were divided into two groups: One was given chloramphenicol (CAP) or thiamphenicol (TAP) in an early stage after marrow transfusion and the other in a later stage. In both groups, more prominently in the former group, some ultrastructural changes were recognized in mitochondria of the early erythroblasts. Mitochondria increased in number and became swollen with electron lucent matrix and paucity of cristae. Condensed mitochondria described in earlier reports were never observed. Intermediate or late erythroblasts seen only in the latter group did not show such alterations. Mitochondrial changes in the early erythroblasts were considered to reflect the dose-related toxic effects of CAP and TAP, and it is assumed that these drugs affect the development of erythroblasts in a very early stage.

Animals↗
PubMed1980

Peripheral contrast echocardiographic findings of anomalous drainage of the right superior vena cava into the left atrium.

A 1-year-old girl had cyanosis and signs of a "normal heart". Peripheral contrast echocardiography (M-mode and real time, two-dimensional) was performed with injection of saline into a superficial vein of the right hand. Contrast echoes did appear in the left atrium and ventricle, but did not in the right artium or ventricle. From these findings anomalous drainage of the right superior vena cava into the left atrium was suspected and was confirmed by cineangiocardiography.

Echocardiography↗
PubMed1980

Leukocyte migration inhibition among breast cancer patients in response to various oncogenic viruses.

The direct leukocyte migration inhibition (LMI) test was done with leukocytes from healthy women and from patients with primary operable breast cancer, benign breast disease, or head and neck cancer. Purified preparations of murine mammary tumor virus (MuMTV), Mason-Pfizer monkey virus (MPMV), and murine leukemia virus (MuLV) were used. For each virus, the lowest 10th percentile of the LMI responses of controls was used to discriminate positive from negative responses. Leukocytes from 46 of 94 (49%) breast cancer patients responded to MuMTV, which was significantly different from each of the other test groups: Positive reactions were observed in only 9 of 67 (13%) healthy persons, 2 of 32 (6%) patients with benign breast tumors, and 2 of 20 (10%) patients with head and neck cancer. Although leukocytes from 29% of the breast cancer patients responded to MPMV, this response did not significantly differ from that observed in healthy women (14%), in patients with benign disease (20%), or in patients with head and neck cancer (20%). The leukocytes from the breast cancer patients were not reactive to MuLV. LMI tests to both MuMTV and extracts of MCF-7 cultured breast cancer cells were done in 36 breast cancer patients and 40 healthy women simultaneously. Of the breast cancer patients, 75% responded to MuMTV and/or MCF-7 antigen as compared to 18% of the controls (P less than 0.005). These data suggest that leukocytes from breast cancer patients are presensitized to MuMTV and antigen(s) present in MCF-7 breast cancer cell line, but not to MPMV or MuLV.

Adult↗
PubMed1980

[Studies on adenocarcinoma and mixed carcinoma of the uterine cervix (author's transl)].

It is known that among the types of cervical cancers three are those which were usually called mixed cancers that indicate various transitional images between pure adenocarcinoma and pure epidermoid carcinoma. No definition for such cancer but mechanism of its histogenesis or its biological nature has been established. From this stand point of view I investigated the tissue and cytological specimens of 284 cases of cervical cancer, and obtained results as follow. 1) The number of tumors showing glandular structure, even if only to a very minor degree, accounted for 32.8% of all frank invasive cancers. On the other hand, tumors with positive mucous reaction made up 61.7%. 2) The frequency of positive mucous reaction increased in relation to the invasion depth. 3) It seems that mixed cancers are derived from three mechanisms--the acanthomatous change from pure adenocarcinoma, the collision of two types cancer and the bipotential differentiation of the same original cells. 4) It may be said that the detailed classification and investigation of cancer cells as observed in cytological specimens may serve to some extent as a clue to the estimation of glandular characteristics of cancer tissues.

Adenocarcinoma↗