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Biomedical subjects

M Fukuda

Publications and source records attributed to M Fukuda.

At least 1,279 records · Page 71Linked to original sources
PubMed1983

Application of the microcomputer in multiparametric fluorescence cytophotometry.

Methods for combined cytofluorometric determinations of nuclear DNA plus other parameters (proteins, enzyme activity, or immunoreactive substances) are reported. DNA measurement was performed on an autoradiograph of Feulgen-stained fetal mouse hepatocytes. In order to facilitate the multiparametric measurements and data processing, a fluorescence cytophotometer (BH2-QRFL, Olympus, Tokyo) equipped with four interchangeable filter-sets and controlled by a desktop computer (HP-85F), was used. All data stored in the same computer were displayed in a two-dimensional scatter plot or two-dimensional frequency distribution histogram. Analysis of the DNA histogram of fetal mouse hepatocytes was accomplished by the same computer. Calculated fraction sizes for G, S, and G plus M phases were found to be in good correlation with those determined directly on the simultaneously combined tritiated thymidine (3H-TdR) autoradiography.

Adult↗
PubMed1983

Leucine-enkephalin-like immunoreactivity in the chicken retina with a special reference to its fine structures.

Leucine-enkephalin-like immunoreactivity (LEI) in the chicken retina was investigated using light and electron microscopic immunohistochemistry. A flat-mount technique for light microscopy clearly demonstrated that LEI cells were distributed widely throughout the entire retina, without any differences between the central and peripheral retinal regions. Frozen sections for light microscopy confirmed the previous findings in pigeons that LEI is localized in amacrine cells. The present electron microscopic observations confirmed the findings of light microscopy and revealed that LEI terminals make synaptic contract mainly with vesicle-containing processes that seemed to belong to the amacrine cells. The present study further suggests that LEI terminals are both pre- and postsynaptic elements onto vesicle-containing processes mentioned above.

Animals↗
PubMed1983

Mitomycin C-induced acceleration of liver cell polyploidization in adult rat after partial hepatectomy.

Mitomycin C, a DNA cross-linking agent, was administered for a week intraperitoneally to a normal 9-week-old rat in which hepatocyte proliferative activity had almost ceased. In the rat treated with mitomycin C, the number of polyploid cells with the DNA amounts more than 4C was not increased in comparison with that in the control rat without any treatment. However, the partial hepatectomy in the rat pretreated with mitomycin C provoked prominent hepatocyte polyploidization much greater in degree than that found in the hepatectomized rat without mitomycin C treatment. These results seem to indicate that the existence of cross-linking DNA damages is a latent factor necessary for the induction of abortive mitosis of a cell after completion of DNA synthesis, which results in the production of a mononuclear polyploid cell in one-step higher DNA class. Cross-linking DNA damages and DNA synthesis are the essential factors for the manifestation of polyploidization.

Animals↗
PubMed1983

A method of DNA histogram analysis by computer and its evaluation by simultaneous combination with 3H-thymidine autoradiography.

A method for determining the fractions of cells in the G1, S, and G2 + M phases of the cell life cycle, by quantifying DNA histograms derived from static fluorescence cytophotometry, was evaluated by simultaneous combination with 3H-thymidine autoradiography. DNA histograms were obtained by cytofluorometry on the Feulgen-stained autoradiographs of HeLa cells, and mouse and rat hepatocytes, after DNA labelling with 3H-thymidine. The synthetic histogram determined by "sum of discrete normal curves" technique was fitted to the experimental data according to a weighted least-squares method by a desk-top computer (HP 85F). The mean relative percent deviations of estimated cell cycle phase fractions from the actual phase fractions determined directly on an autoradiograph was 6.6 +/- 3.3%.

Aging↗