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Biomedical subjects

M Frey

Publications and source records attributed to M Frey.

At least 271 records · Page 15Linked to original sources
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Structure and sequence of the multihaem cytochrome c3.

The molecular structure of cytochrome c3 isolated from Desulfovibrio desulfuricans has been solved on the basis of its crystallographic determination at 2.5 A resolution and of an essentially complete sequence. The molecule consists of a single polypeptide chain wrapped around a very compact core of four non-parallel haems which present a relatively high degree of exposure to the solvent. Alignment of the amino acid sequences of cytochrome c3 from several species suggests that the structure reported here is characteristic of the cytochrome c3 group.

Amino Acid Sequence↗
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[Prevention of para-articular ossifications by radiotherapy after cementless total hip endoprosthesis implantation].

By means of a prospective study, concerning the postoperative rate of ossification after cementless total hip replacement, it was due to prove the efficacy of radiotherapy in preventing periarticular ossification. In 1992 arthroplasty was followed by radiotherapy of 50 hip joints as regular therapy. The radiation was performed with a focal dose of 8 Gy. Patients with bilateral cementless total hip replacement and radiotherapy only at one side were of special interest in this study. Within the 24th postoperative week in 28 (56%) of the radiated hip joins no periarticular ossifications were found. In 20 (40%) we found ossifications grade 1, in 1 case ossification grade II and in 1 further case ossification grade III following the classification of Arcq. By 8 patients with former cementless total hip replacement without postoperative radiotherapy, a significant reduction of the ossification rate was found in the contralateral hip joint treated by postoperative radiotherapy. The rate of ossification was reduced by 28%. In correlation to reduction of periarticular ossification the increase on the overall range of motion in the radiated hip joins was 10.9%. By none of the patients treated by radiotherapy we found a disturbed healing process, a deep infection or an early loosening of the endoprosthesis. Postoperative radiotherapy as regular therapy for prophylaxis of periarticular ossification after Cementless total hip replacement can subsequently be recommended.

Adult↗
PubMedDate not supplied

[Functional electrostimulation of paraplegic patients--1 year's practical application. Results in patients and experiences].

In October 1982 two patients suffering from paraplegia ware, world wide for the first time, implanted two 8-channel-stimulation-devices at the II. Surgical Clinics Vienna. Four electrodes were fixed to the nervus femoralis and the nervus gluteus inferior with the help of microsurgical techniques. The aim of the operation is to enable the paralysed patient to "stand up himself" and to "cover a short distance on crutches". They must learn to manipulate the implanted "more-channel-device" with their fingers, and to coordinate this with the supporting function and the balance function of their arms. The wheel-chair shall not and cannot be substituted with this method: Not without, but with wheel-chair and functional electrostimulation. A comprising training-programme for building up muscles, balance control and gait training was necessary. After 4 months muscle-force and endurance had increased 200%. The patients could stand up stimulatedly without help between parallel bars. At the rehabilitation center Bad Häring the first steps were done in March 1983 (= 5. month after operation). The greatest success was when a patient could get stimulatedly out of her car and do the through-swing-gait over a distance of 60 meters. After one year the muscle force was quadrupled, the endurance was increased 20-30 times, depending on the type of gait. Both patients are able to walk in the 4-P-gait or swing-through-gait on crutches over short distances without help from others. Riding a bicycle stimulatedly on even ground was demonstrated.

Adult↗
PubMedDate not supplied

[Clinical and electroneurographic results following surgical treatment of the ulnar nerve sulcus syndrome].

The sulcus of the elbow-joint represents a bottleneck which may cause compression of the ulnar nerve. We studied clinical and electroneurographical results in 26 patients after operation of the sulcus ulnaris-syndrome. The influence of the duration of compression as well as additional illnesses was evaluated on the postoperative result. The surgical treatment was an anterior transposition of the nerve in a subcutaneous layer combined with external neurolysis. Due to the symptoms and the electroneurographical results after operation the patients were divided into three groups. The best results were obtained in cases with a short period of preoperative complaints (below 1 year). Although heavy work had a positive effect on the manifestation of a sulcus ulnaris-syndrome, it had no adverse effect if started again after operation. Anterior subcutaneous transposition is the operative procedure of choice in cases of sulcus ulnaris-syndrome.

Adult↗
PubMedDate not supplied

TNK-tissue plasminogen activator compared with front-loaded alteplase in acute myocardial infarction: results of the TIMI 10B trial. Thrombolysis in Myocardial Infarction (TIMI) 10B Investigators.

BACKGROUND: Bolus thrombolytic therapy is a simplified means of administering thrombolysis that facilitates rapid time to treatment. TNK-tissue plasminogen activator (TNK-tPA) is a highly fibrin-specific single-bolus thrombolytic agent. METHODS AND RESULTS: In TIMI 10B, 886 patients with acute ST-elevation myocardial infarction presenting within 12 hours were randomized to receive either a single bolus of 30 or 50 mg TNK-tPA or front-loaded tPA and underwent immediate coronary angiography. The 50-mg dose was discontinued early because of increased intracranial hemorrhage and was replaced by a 40-mg dose, and heparin doses were decreased. TNK-tPA 40 mg and tPA produced similar rates of TIMI grade 3 flow at 90 minutes (62.8% versus 62.7%, respectively, P=NS); the rate for the 30-mg dose was significantly lower (54.3%, P=0.035) and was 65. 8% for the 50-mg dose (P=NS). A prespecified analysis of weight-based TNK-tPA dosing using median TIMI frame count demonstrated a dose response (P=0.001). Similar dose responses were observed for serious bleeding and intracranial hemorrhage, but significantly lower rates were observed for both TNK-tPA and tPA after the heparin doses were lowered and titration of the heparin was started at 6 hours. CONCLUSIONS: TNK-tPA, given as a single 40-mg bolus, achieved rates of TIMI grade 3 flow similar to those of the 90-minute bolus and infusion of tPA. Weight-adjusting TNK-tPA appears to be important in achieving optimal reperfusion; reduced heparin dosing appears to improve safety for both agents. Together with the safety results from the parallel Assessment of the Safety of a New Thrombolytic: TNK-tPA (ASSENT I) trial, an appropriate dose of this single-bolus thrombolytic agent has been identified for phase III testing.

Aged↗
PubMedDate not supplied

Evidence for uptake of plamid DNA into intact plants (Lemna perpusilla) proved by an E. coli transformation assay.

The water plant Lemna perpusilla has been incubated with the E. coli plasmids pMB9 and pBR325, respectively. Uptake of plasmids has been shown by subsequent transformation of E. coli cells to tetracycline resistance after treatment with Lemna DNA from plasmid-incubated plants. In 7 out of 15 assays we found stable transformants. From the transformation rate an amount of 10(-4) to 10(-6) micrograms plasmid DNA per 10 micrograms of plant DNA can be calculated.

DNA, Recombinant↗
PubMedDate not supplied

[Quantifying facial asymmetry. A method for objective assessment of impairments of facial motoricity; a pilot study].

The aim of this study was to develop a method of objectively evaluating the results of a dynamic reconstruction of facial nerve paralysis by means of nerve transplants with a view to reinnervating a free muscle transplant. The Vicon 3 D motion analysis system was used to measure the distances and movements of marker points on the face of the patient. This objective technique permits evaluation of the operation as well as the results of treatment.

Adult↗