Biomedical subjects
Publications and source records attributed to M Flores.
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Nepeta sibthorpii Bentham (Lamiaceae) is an herbaceous aromatic plant, growing in dry, usually rocky areas of Southern Greece. In this paper, we report the study of micromorphological features of the flowers and leaves of Nepeta sibthorpii Bentham. The analysis by means of scanning electron microscopy has permitted to characterize both the covering trichomatous garment, abundantly present on the calyx and on the leaves, and the glandular hairs, distributed on the adaxial leaf surface and on the outer surfaces of calyx and corolla, showing also the peculiar features of the epidermal cells, all useful diagnostic elements for the identification of this medicinal plant.
Entada africana Guill. et Perr., known in Mali by the common local name 'Samanéré' in the Bambara language, is one of the traditional Malian medicines prescribed for many illnesses. In the present investigation, micromorphological studies were carried out by scanning electron microscopy on the roots and the leaves. The correct identification of the morphological characters of drugs is of great interest for quality control in basic research and drug production, especially for imported items and for raw materials sold by traditional herbalists.
In order to characterize the Major histocompatibility complex (MHC) class II A genes of the channel catfish (Ictalurus punctatus) a cDNA library was screened and PCR was performed. Four different full-length cDNA sequences for MHC class II A genes were obtained from a clonal B cell line derived from an outbred fish. Two different genomic sequences and corresponding cDNAs were obtained from a presumably homozygous gynogenetic catfish. The A genes have five exons and four phase one introns. The first exon encodes the 5' untranslated region (UTR) and leader peptide; the second and third exons encode the alpha1 and alpha2 domains, respectively. The connecting peptide, transmembrane and cytoplasmic domains, as well as part of the 3' UTR, are encoded by the fourth exon and the rest of the 3' UTR is encoded by the fifth exon. Southern blot analyses using an exon three probe revealed two to four hybridizing fragments with considerable restriction fragment length polymorphisms evident among randomly selected outbred channel catfish. These findings are consistent with the presence of at least two functional polymorphic MHC class II A gene loci. An unusual aspect of the channel catfish MHC class II alpha chain is its lack of N-linked glycosylation sites.
Concentrations and types of bacteria and fungi in the air and on surfaces were measured in a new, one-bedroom apartment before occupancy and at 3-mo intervals thereafter for 2 y. Air samples were collected with a single-stage impactor at two locations in the kitchen, living room, bedroom, bathroom, and outdoors. Even though damp surfaces supported large populations of bacteria and yeasts, the air concentrations in the kitchen and bathroom were similar to those in other rooms. No changes in concentrations or types of microbiological contamination were observed between the first and second years of occupancy, but elevated outdoor humidity, lower air temperature, and winds from the northeast were associated with higher bacterial and fungal air concentrations. The median air concentrations of bacteria were 98 cfu/m3 (95% confidence interval 26-372 cfu/m3) indoors and 101 cfu/m3 (28-364 cfu/m3) outdoors. The median air concentrations of fungi were 198 cfu/m3 (58-673 cfu/m3) indoors and 362 cfu/m3 (113-1158 cfu/m3) outdoors. This information established useful baseline data against which measurements in neighboring complaint residences could be compared.
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The effect of irradiation on the anti Ig binding ability of lymphocytes after irradiation was studied. Normal control and irradiated lymphoid cells were treated with rabbit anti mouse IgG fluorescein conjugated serum and the fluorescein positive cells compared. A reduction in the proportion of stained cells in the irradiated lymphocytes was found. It depends on the dose and on the incubation time at 37 degrees C after irradiation. The maximum effect was detected after 10 minutes of incubation, longer incubation is associated with a partial recovery of the stainability of the IgG receptors.
BACKGROUND: Uveitis is considered an autoimmune disease that compromises any internal structure of eye; its main characteristic is the inflammatory process inside eye. OBJECTIVE: To determine which is the most effective therapy for autoimmune uveitis: cyclophosphamide, azathioprine and prednisone. MATERIAL AND METHODS: Patients between 16 and 65 years old, both sexes, with diagnosis of autoimmune uveitis (anterior, intermediate and posterior). Patients were submitted to clinical history, physical exploration and clinical studies. Their written informed consent was requested and patients were divided into three treatment groups: group 1 (azathioprine) 14 (42.4%) patients; group 2 (prednisone) 15 (45.5%) patients, and group 3 (cyclophosphamide) 4 (12.1%) patients. Blind double observations and assessments at day 0 and at week 1, 2, 4 and 6 of treatment were performed. RESULTS: Out of 33 patients studied, 13 (39.4%) were male and 20 (60.6%) female, the most frequent diagnosis was uveitis due to Toxoplasmosis. With azathioprine 100 mg/day it was observed a better and faster control of inflammation after 6.2 weeks at anterior chamber with p < 0.0001, with prednisone, p = .000, and with cyclophosphamide, p = 135. In the posterior chamber there were no significant inflammatory changes for three groups with p = .353. The most frequent adverse effects occurred in the prednisone group with p < 0.05 in 24.2%, characterized by neutropenia and urinary tract infection. CONCLUSIONS: Azathioprine, 100 mg/day oral during six weeks, is considered the most effective drug, since it demonstrated significant diminishment of inflammatory process in less time, with less side effects and with conservation of visual acuity.