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Biomedical subjects

M Fabri

Publications and source records attributed to M Fabri.

At least 37 records · Page 2Linked to original sources
PubMed1990

Calcitonin gene-related peptide-positive neurons and fibers in the cat dorsal column nuclei.

An antiserum raised against the C-terminal region of rat alpha-calcitonin gene-related peptide has been used to investigate the morphology and topographical distribution of neurons and terminals containing calcitonin gene-related peptide in the cat dorsal column nuclear complex. Calcitonin gene-related peptide-positive fibers and axon terminals were denser in the cuneate nucleus than in the other dorsal column nuclei subdivisions and were observed throughout all rostrocaudal levels. They were densest in the dorsal and ventrolateral portions of the middle cuneate. Immunoreactive neurons were observed only in animals pre-treated with colchicine. In these cases, some calcitonin gene-related peptide-positive neurons were present in the cuneate and in the external cuneate. In double-labeling experiments, visualization of calcitonin gene-related peptide immunoreactivity in dorsal root ganglia neurons was combined with the retrograde transport of colloidal gold-labeled wheat germ agglutinin conjugated to inactive horseradish peroxidase injected in the cuneate nucleus. These experiments show that calcitonin gene-related peptide-positive fibers in the cuneate nucleus originate mostly from C3-C6 medium sized dorsal root ganglia neurons but also from some small and large neurons. These results suggest that calcitonin gene-related peptide-positive fibers may convey sensory information from a wide range of peripheral receptors.

Animals↗
PubMed1990

[Acute gastrointestinal hemorrhage after placement of a peritoneojugular shunt].

Since 1984 the peritoneovenous (LeVeen) shunt has been installed in 33 patients (10 females 30.3%, and 23 males 69.7%), with the average age of 54 +/- 8 years all in the oedema--ascitic decompensation phase of their primary illness. The control group consisted of 39 patients with identical etiology and primary illness stadium, sex and age structure and duration of primary illness, all treated with medicament - diet therapy. All the operated patients and those treated with medicament--diet regime died. Their autopsy findings were confronted. The aim of this study was to indicate the type and frequency of digestive hemorrhage in this population on our own clinical--patient material. Gastrointestinal hemorrhage was the cause of death in 8 (24.24%) patients out of the group operated on, and 6 (15.3%) from the control group. The isolated rupture of esophageal varices was the cause of death in one (3.03%) patient out of the group operated on, and in 5 (12.12%) patients from the control group, the rupture of esophageal varices with toxic vasculitis in 3 (0.09%) patients from the group operated on, and not in one out of the control group; rupture of esophageal varices associated with toxic vasculitis and disorders of the hemostatic mechanism appeared in 4 (12.12%) of patients from the group operated on, and in 1 (2.56%) of the control group. On the basis of the presented it can be concluded that gastrointestinal hemorrhage is a significantly more frequent cause of death in the group operated on than in the control group, and that hemorrhages are of an all the more complex etiology when the flow-in of ascites into circulation is greater. Hemodilution and endotoxins are to blame for their occurrence and it is necessary to preoperatively conduct, beside the control of the hemostatic mechanism, also the test of ones own ascitic burdening and to determine the level of endotoxin in the ascites.

Acute Disease↗
PubMed1990

[Therapy of resistant ascites using the peritoneojugular (Leveen shunt)].

Since 1984 the peritoneovenous shunt has been installed in 33 patients (10 females - 30.3%; 23 males - 69.7%) of the average age of 54 +/- 8, all in the phase of therapeutically resistant ascites (alcoholic cirrhosis 28 - 84.85%; 4 - 12.12% posthepatitic cirrhosis; and 1 - 3.03% hepatic amyloidosis). The control group consisted of 39 patients (11 females - 28.2% and 28 males - 71.8%) treated in an identical time span with the strict conduction of medicament-diet therapy. The aim of this study was to check the value of this method on our own clinical-patient material, and therefore establish the incidence of complications. By the use of a unique protocol we followed mortality, morbidity, body weight, belly circumference, diuresis, the ultrasonographic finding of the abdominal cavity and the complications which appeared. Out of the group operated on 19 (57%) of the patients died, and so did all the control group patients as well. The average life duration was 275 +/- 810 days in the group operated on, and 44 +/- 29 (p less than 0.005) in the control group. All those alive (14.33-42.42%) lived longer than six months. Six patients lived longer than one year (42.85%), 4 (28.47%) longer than two years, and one (7.14%) longer than three years. There is a statistically significant decrease in body weight, belly circumference, diuresis increase and the consequent ascites withdrawal. DIC occurred in 2 patients, shunt malposition in 2, saccular dilatation in 1, plastic peritonitis in 6, and ileus in 1 patient. Not one of the listed complications resulted by death.(ABSTRACT TRUNCATED AT 250 WORDS)

Ascites↗
PubMed1990

[Partial heterotopic and total orthotopic transplantation of the liver in dogs].

From 1983 to 1988 on the Department for Experimental Surgery 20 preservations of the liver has been done, with ice cold Euro- Collins and Ringer solutions, either with in situ or with on banch technique after fast expantation. On five dogs standard 55% left hepatectomy has been done and the right side preserved in situ. Partial heterotopic liver transplantation to the right iliac fossa was performed on five dogs. Total vascular exclusion of the liver and extracorporal inierior vena cava and jugular vein by pass with assisted circulation was performed on one dog, without assistance on four dogs. Total orthotopic liver transplantation was performed on two dogs. The surgical team practiced the technique of liver transplantation in experiment and liver resection with in situ technique and on banch technique.

Animals↗
PubMed1990

[Experimental study of liver allograft damage during cold ischemia].

Changes on the majority of structural complexes were observed in livers that underwent the influence of the preservant and cold ischemia. Cellular architecture of a such liver is disturbed. Hepatocites are with granular and vacuolar degeneration: focal cariolyzes and piasmolyzes are present, partial destruction of cellular membrane is noticeable. The dilatation of sinusoidal spaces is very emphasized and followed by the severe loss of endothelium. These changes along with the disturbed cellular architecture represent the findings of Pelosis hepatis. It is important to emphasize the significance of changes observed on liver sinusoids and endothelium especially since their intensity is much greater than of those changes observed on parenchim. We assume that these angioarchitectural damages play an important role in the onset and development of cardiopulmonal and coagulopatic components of the postreperfusional syndrome. The fact that the postreperfusional syndrome was also observed in cases with heterotopic transplantation could support this assumption.

Animals↗
PubMed1990

[Damage in the hemato-enteral barrier in experimental liver transplantation].

Semiquantitative analyzes of intestinal mucous lesion graduating from 0 (normal) to V (necrotic) were done in the experimental model, 6-12h alter the heterotopic liver transplantation in dogs. All degrees of mucous damages were found in intestinal samples (mean value was 2.60). The most dominant were the lesions of III (31%) and IV (25%) degree while in 4% complete destruction of mucousal villious was observed. Success of liver transplantation besides the presence of the lesions of hepatic alograft during the phases of the warm and cold ischemia or immunological responses, depends on the changes in functionally connected regions. In that sense the damages of mesenterial department lead a very important role. Lumenal infective agents and cardiodepressive macromolecules, passing trough the hematoenteral barrier, cause local hepatic damages and central cardiovascular failure.

Animals↗
PubMed1988

Immunocytochemical evidence for glutamatergic cortico-cortical connections in monkeys.

Retrograde transport of horseradish peroxidase and immunocytochemical visualization of glutamate (Glu) were combined to investigate the neurotransmitter used by cortico-cortical neurons in the first (SI) and second (SII) somatic sensory areas of macaque monkeys. The majority of association and callosal neurons in SI and SII were immunoreactive for an antiGlu serum: evidence was therefore obtained in support of a role for Glu, or a closely related compound, as the synaptic transmitter used by cortico-cortical fibers.

Animals↗
PubMed1988

Cytochrome oxidase histochemistry reveals regional subdivisions in the rat periaqueductal gray matter.

The identification of different anatomical regions of the periaqueductal gray matter of rats was addressed in the present study by using the histochemical staining for the mitochondrial enzyme cytochrome oxidase. At caudal and middle levels, cytochrome oxidase histochemistry clearly demonstrates the existence of four subdivisions: dorsal, dorsolateral, ventrolateral and medial, whereas in sections from the rostral periaqueductal gray matter only two concentric bands are identifiable on the basis of the degree of cytochrome oxidase activity.

Animals↗
PubMed1988

Glutamate-positive corticocortical neurons in the somatic sensory areas I and II of cats.

Combined retrograde transport-immunocytochemical experiments were carried out on cats to study the morphology, laminar distribution, and percentages of corticocortical projecting neurons of somatosensory area I (SI) and II (SII) showing immunoreactivity to an antiserum raised against the amino acid glutamate (Glu). A previously characterized anti-Glu serum (Conti et al., 1987a, b; Hepler et al., 1987) was used in conjunction with HRP. This tracer was injected either in SI to label retrogradely neurons in ipsilateral SII (SII-SI association neurons) and contralateral SI (SI-SI callosal neurons) or in SII to label retrogradely neurons in ipsilateral SI (SI-SII association neurons) and contralateral SII (SII-SII callosal neurons). In sections from SI and SII processed for simultaneous visualization of Glu and HRP (Bowker et al., 1982), and containing the cells from which every one of the 4 corticocortical projections arise, 3 types of labeled neurons were observed: (1) single-labeled neurons showing the homogeneous brown immunoreaction product of Glu (Glu-positive neurons); (2) single-labeled neurons containing the granular black reaction product of retrogradely transported HRP (Glu-negative, association or callosal neurons); and (3) double-labeled neurons in which both the black HRP granules and the brown immunostaining were present (Glu-positive, association or callosal neurons). Double-labeled neurons were all pyramidal in shape and were distributed intermingled with Glu-negative corticocortical neurons in all layers of SI and SII known to give rise to association and callosal projections. Counts from 25-micron-thick sections showed that of 432 association and callosal neurons sampled from SI and SII, 214 (49.5%) were Glu-negative and 218 (50.5%) Glu-positive. In counts carried out on 5-micron-thick sections, the percentage of Glu-positive corticocortical neurons raised to about 70%. The 2 populations of single- and double-labeled corticocortical neurons showed no difference in their perikaryal cross-sectional areas. The present results show that a large fraction of association and callosal neurons of SI and SII are immunoreactive for Glu, and, therefore, these neurons probably use this excitatory amino acid, or a closely related compound, as neurotransmitter.

Animals↗