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Biomedical subjects

M Endoh

Publications and source records attributed to M Endoh.

At least 379 records · Page 21Linked to original sources
PubMed1984

Detection of IgA1-dominant immune complexes in peripheral blood polymorphonuclear leukocytes by double immunofluorescence in patients with IgA nephropathy.

The amounts of IgA1- and/or IgA2-dominant immune complexes included in peripheral blood polymorphonuclear cells (PMN) were determined by the double immunofluorescence technique in patients with IgA nephropathy. The aim of the present study was to determine the prevalence of IgA1-and/or IgA2-dominant immune complexes phagocytized by PMN in patients with IgA nephropathy. 5 patients with IgA nephropathy and 10 healthy adults were examined. It was demonstrated that the percentages of IgA1 with C3 cytoplasmic inclusion bodies were significantly increased compared with those of IgA2 with C3 cytoplasmic inclusion bodies in patients with IgA nephropathy. It was suggested that IgA1-dominant immune complexes are phagocytized by peripheral blood PMN in patients with IgA nephropathy.

Adult↗
PubMed1984

Somatostatin-like immunoreactivity in mammalian thyroid glands: contents and partial characterization.

Somatostatin (SRIF)-like immunoreactivity (SLI) in the thyroid glands of human and several animal species were compared, and the SLI peptides were characterized chromatographically and immunologically. All specimens were extracted with 2 M acetic acid, and the SLI content determined by RIA. The SLI concentrations in guinea pigs [34.3 +/- (SE) 4.8 ng/mg protein] and rabbits (9.4 +/- 0.8 ng/mg protein) were much greater than those in other mammals: dogs, rats, mice, and humans. On gel filtration of extracts of the guinea pig, rabbit and dog thyroids, the major peak of SLI (1.6 K SLI) coeluted with synthetic SRIF-14 (S-14). Two other forms of SLI ("big" SLI and 3 K SLI) were also detected, although their relative proportions to total SLI were small (2.3 to 8.2%). The 3 K SLI and 1.6 K SLI from guinea pig and rabbit thyroids contained peptides coeluting with synthetic SRIF-28 (S-28) and S-14, respectively, on reverse-phase high performance liquid chromatography. The dilution curves of the two molecular forms of SLI, i.e. 3 K SLI and 1.6 K SLI, were parallel to the displacement curves of S-28 and S-14 in the SRIF RIA. It is concluded 1) that the thyroid contents of SLI varied greatly from species to species, with the highest content being found in guinea pig thyroids; 2) that in guinea pigs, rabbits, and dogs, the predominant form of thyroid SLI is 1.6 K SLI; and 3) that the 3 K SLI and 1.6 K SLI peptides from guinea pig and rabbit thyroids are immunologically and chromatographically indistinguishable from S-28 and S-14, respectively.

Animals↗
PubMed1984

Involvement of cyclic AMP in the positive inotropic effect of OPC-8212, a new cardiotonic agent, on the canine ventricular muscle.

OPC-8212 is a new positive inotropic drug which scarcely produces an increase in heart rate and vasodilatation. The positive inotropic effect of OPC-8212 was investigated in relation to cyclic AMP metabolism in canine isolated ventricular muscle. The positive inotropic effect of OPC-8212 was accompanied by accumulation of cyclic AMP in the tissue: the cyclic AMP level was elevated before the increase in force of contraction and elevated in a concentration-dependent manner in the presence of a beta-adrenoceptor antagonist, atenolol (10(-6) M). The OPC-8212-induced increases in force of contraction and cyclic AMP level were abolished by carbachol (3 X 10(-6) M). The relationship between the force of contraction and cyclic AMP level in the presence of OPC-8212 was changed by neither carbachol nor isoproterenol. OPC-8212 enhanced the positive inotropic effect of isoproterenol as cyclic AMP phosphodiesterase inhibitors did. OPC-8212 shortened the time to peak tension and had a tendency to shorten the relaxation time of single contractions. These results altogether suggest that change in cyclic AMP metabolism is involved at least in the positive inotropic effect of OPC-8212 on canine ventricular muscle.

Animals↗
PubMed1984

ACTH-mediated effect of metoclopramide on plasma pregnenolone in man.

Five healthy adult men were given metoclopramide (10 mg and 20 mg) iv and the effects of L-dopa and dexamethasone on metoclopramide-induced increases in the plasma pregnenolone concentration were determined. After an injection of 10 mg metoclopramide, the pregnenolone level rose slightly, though not significantly vs a control. After injecting 20 mg metoclopramide, the pregnenolone level rose significantly vs both the control and the basal level. The pregnenolone increase was not inhibited by L-dopa pretreatment, whereas pretreatment with dexamethasone did suppress it significantly. The data suggest that metoclopramide increases pregnenolone secretion through an ACTH-dependent effect and that dopamine does not modulate pregnenolone secretion in man. This means that dopaminergic modulation does not affect the early steps (cholesterol to pregnenolone) of steroid biosynthesis in man.

Adrenocorticotropic Hormone↗
PubMed1984

Solubilization of intraglomerular deposits of serum proteins by fresh human sera or gamma-globulin in patients with diabetic nephropathy.

The solubilization of renal deposits of IgG and other serum proteins by fresh human sera or human gamma-globulin was studied in patients with diabetic nephropathy. Renal biopsy specimens were from 10 patients with diabetic nephropathy. These specimens were incubated with fresh sera obtained from the same patients and healthy adults or human gamma-globulin at 37 degrees C for 1 hr in plastic test tubes. The sections were stained with FITC-labeled heavy chain specific anti-human IgG, alpha 1-antitrypsin, haptoglobin and beta-lipoprotein antisera, and then examined with a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulin significantly solubilized glomerular deposits of IgG and other serum proteins in patients with diabetic nephropathy. It was postulated that solubilization of protein deposits in glomeruli requires the same substances detected in vitro in the kidney tissues from patients with diabetic nephropathy.

Adult↗
PubMed1984

Solubilization of intraglomerular deposits of IgG immune complexes by human sera or gamma-globulin in patients with lupus nephritis.

A study of the solubilization of glomerular deposition of IgG immune complexes by sera from patients with lupus nephritis is described. Renal biopsy specimens were obtained from 11 patients with lupus nephritis, five patients with IgA nephropathy and one patient with minimal change nephrotic syndrome. These renal specimens were incubated with fresh, stored or heated sera from the same patients or healthy adults and human gamma-globulins at 37 degrees C for 1 h in plastic test tubes. The sections were stained with FITC conjugated heavy chain specific anti-human IgG or C3 antisera and then examined with a fluorescent microscope. The sections were also stained with FITC conjugated human gamma-globulins and rhodamine conjugated anti-human IgG, IgM or IgA antisera and then examined by double exposure under a fluorescent microscope. It was demonstrated that fresh human sera or gamma-globulins significantly solubilize glomerular immune deposits in patients with lupus nephritis in vitro. It was indicated that the solubilization of IgG glomerular deposits from patients with lupus nephritis does not depend on complement. It is postulated that solubilization of immune deposits in glomeruli requires the excess amounts of antigenic substances in patients with lupus nephritis.

Antigen-Antibody Complex↗
PubMed1984

Cold reacting anti-nuclear factor (ANF) in families of patients with IgA nephropathy.

The emergence of cold reacting anti-nuclear factor (ANF) in families of patients with IgA nephropathy was examined to determine whether some immunological alterations among family members affect the development of this disease. The procedure for the detection of the cold reacting ANF was reported previously. Fifty-five per cent of sera from 66 relatives of IgA nephropathy 24 patients was found to have the IgM cold reacting antibody. The incidence of ANF in healthy adults was 3%. Both household and non-household consanguineous relatives showed antibody in their sera. Sixty-five per cent of consanguineous relatives who had close household contacts with IgA nephropathy patients showed cold reacting ANF, whereas only 10% of non-consanguineous relatives who had close household contact had this antibody. It is suggested that familial susceptibility or genetic factors, in addition to environmental factors, may be responsible in the development of IgA nephropathy.

Adolescent↗
PubMed1984

Two cases of acute poststreptococcal glomerulonephritis superimposed on rheumatoid arthritis.

Recently, the rheumatoid factor (RF) has been postulated to play a role in the development of acute poststreptococcal glomerulonephritis (APSGN). However, there are few reports in which APSGN is superimposed on rheumatoid arthritis (RA). Two patients are reported in this paper who showed atypical renal histopathological findings as APSGN. It was suggested that renal histopathological finding might become different from those of typical APSGN when it is superimposed on RA.

Adult↗
PubMed1984

Mode and mechanism of action of 3,4-dihydro-6-[4-(3,4-dimethoxybenzoyl)-1-piperazinyl]-2(1H)- quinolinone (OPC-8212), a novel positive inotropic drug, on the dog heart.

Mode and mechanism of action of 3,4-dihydro-6-[4-(3,4- dimethoxybenzoyl )-1-piperazinyl]-2(1H)- qu inolinone ( OPC -8212) were investigated exclusively on dogs both in vivo and in vitro. When assessed by intra-arterial administration in isolated, blood-perfused papillary muscle, sinoatrial node and atrioventricular (AV) node preparations, OPC -8212 produced a positive inotropic effect scarcely causing either positive or negative chronotropic and coronary vasodilator effects. AV nodal conduction was slightly facilitated with OPC -8212, whereas ventricular automaticity was rather suppressed. No arrhythmias were produced. Thus, OPC -8212 is highly specific for force. In heart-lung preparations OPC -8212 improved cardiac function depressed with pentobarbital, and caused bradycardia. In trabecular muscles of the right ventricle (hereafter simply called ventricular muscles) OPC -8212 produced a concentration-dependent positive inotropic effect in the presence of the beta 1-adrenoceptor antagonist, atenolol. This indicates that the positive inotropic effect of OPC -8212 is not mediated through a beta-adrenoceptor mechanism. In ventricular muscles OPC -8212 increased plateau potentials of normal action potentials in the presence of atenolol. In K+-depolarized ventricular muscles OPC -8212 increased overshoot potentials and durations of slow response action potentials in the presence of atenolol. These suggest an increase in the slow inward current to underlie the positive inotropic effect of OPC -8212. In ventricular muscles OPC -8212 shortened total duration of contraction and time to peak tension in the presence of atenolol. In ventricular muscles OPC -8212 increased force of contraction and intracellular concentrations of cyclic AMP in a parallel way in time course in the presence of atenolol.(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗
PubMed1984

In vivo alteration of antibody production in patients with IgA nephropathy.

Augmentation of IgA production has been postulated for the development of IgA nephropathy. An influenza HA vaccine was administered to healthy adults and patients with IgA nephropathy to elucidate if there was any in vivo alteration of antibody production in response to antigenic stimulation in these patients. The vaccine was administered s.c. in a dose of 350 CCA units at an interval of 4 weeks. IgG, IgA and IgM class antibodies to influenza HA antigens and three classes of rheumatoid factors (RF) were determined using a solid phase fluorescence immunoassay. The titres of IgG class antibodies to influenza HA antigens did not change significantly in either group after the vaccination. No significant differences were observed in the titres of IgG antibodies between the two groups. IgA antibodies were significantly increased only in patients in the 4th week and continued to the 8th week. The titres of IgA antibodies were always higher in patients than in controls during the study period. IgM antibodies were significantly increased stepwise in both groups to an equal degree. IgG and IgA RF were always higher in patients than in controls. IgM RF were significantly increased and higher than in controls in the 8th week in patients. It is concluded that patients with IgA nephropathy might be high responders for IgA antibody production, and that polyclonal activation might be associated with increased IgA production following in vivo antigenic stimulation in these patients.

Adult↗
PubMed1984

Aberration of B and T cell subsets in patients with IgA nephropathy and membranous nephropathy.

Peripheral blood lymphocytes from 15 patients with IgA nephropathy (IgAN), 10 patients with membranous nephropathy (MN) and 15 healthy adults were examined to investigate whether there are any aberrations in B and T cell subsets in these diseases. F (ab')2 portions of heavy chain specific anti human IgG, IgA and IgM antibodies were used to determine isotypes of B lymphocytes, and monoclonal antibodies directed at human T cells termed OKT3, OKT4 and OKT8 antibodies were used to assay subsets of T cells. Quantitation of B and T cell subsets was performed by flow cytometry. IgG, IgA and IgM bearing peripheral blood lymphocytes were significantly increased in patients with both IgAN and MN, although the dominant class of immunoglobulin bearing cells in patients with IgAN was IgA bearing cells and that in patients with MN was IgM bearing cells. OKT4 positive (OKT4+) and OKT8 positive (OKT8+) cells were significantly decreased in both patient groups. The OKT4+/OKT8+ ratio increased only in MN patients without steroid therapy. It was suggested that aberrations of B and T cell subsets are common in both diseases.

Adolescent↗
PubMed1984

A case of mesangial IgM nephropathy with decreased renal function.

A case of mesangial IgM nephropathy associated with chronic renal failure is described. The patient did not reveal nephrotic syndrome during the clinical course. Renal biopsy specimens revealed typical features of mesangial IgM nephropathy when studied by light microscopy, electron microscopy and immunofluorescence staining. Mesangial IgM nephropathy is considered to be a heterogenous disorder, because this disorder is clinically characterized by nephrotic syndrome, mild proteinuria and/or hematuria or renal failure.

Autoimmune Diseases↗
PubMed1984

Effects of a "single shot" of urokinase on fibrinolytic activities in patients with IgA nephropathy.

A study on the clinical effects of urokinase in patients with IgA nephropathy is described. Three different methods of administration, including single, continuous, and mixed administration, were employed in this study. Measurements of plasminogen, plasmin and alpha 2-plasmin inhibitor levels in plasma were performed during the course of urokinase administration in patients with IgA nephropathy and chronic proliferative glomerulonephritis. Measurements of alpha 1-anti-trypsin and alpha 2-macroglobulin levels were also performed in these patients. Urinalysis was performed both before and after administration of urokinase. It was demonstrated that a single shot of urokinase induced a significant fibrinolytic activity in patients with IgA nephropathy, and that a single shot of urokinase was effective in improving proteinuria and/or hematuria in patients with IgA nephropathy. It is concluded that a single shot of urokinase may be useful for treatment of patients with IgA nephropathy.

Fibrinolysis↗
PubMed1984

Two adult cases of ectopic kidney.

Two patients with ectopic kidney are described here. One was a 33-year-old male with hypertension and heavy proteinuria. The other was a 30-year-old male who had hypospaedia complained of dysuria for 30 years. These two cases were correctly diagnosed after radiographical examinations. Early detection of this disease can be achieved by using some radiographical techniques which is important in managing the future course of patients with ectopic kidney.

Adult↗
PubMed1984

Increase in proteinuria and/or microhematuria following upper respiratory tract infections in patients with IgA nephropathy.

Exacerbation of urinary abnormalities after upper respiratory tract infections in patients with IgA nephropathy was examined. Twenty patients with IgA nephropathy and fourty patients with other glomerular diseases were evaluated in this study. The incidence of exacerbation in microhematuria after upper respiratory tract infections was significantly higher in patients with IgA nephropathy than those with other glomerular diseases. The improvement of microhematuria after upper respiratory tract infections was significantly delayed in some patients with IgA nephropathy. It is indicated that upper respiratory tract infections might be a risk factor of exacerbation in patients with IgA nephropathy.

Glomerulonephritis↗
PubMed1984

Detection of polymeric IgA in sera from patients with IgA nephropathy determined by thin-layer gel filtration.

Polymeric IgA in sera was determined by thin-layer gel filtration in patients with IgA nephropathy, other glomerular diseases, IgA myeloma and healthy adults to determine whether serum IgA is composed of a monomeric and/or larger polymers in these patients. The levels of IgA in sera were also quantitated by laser nephelometer in these patients. Eleven patients with IgA nephropathy (Berger's disease), six patients with other glomerular diseases, three patients with IgA myeloma and four healthy adults were examined. It was shown that the polymerized IgA in sera from patients with IgA nephropathy was significantly higher than that in sera from patients with other glomerular diseases and healthy adults. Levels of IgA in sera from patients with IgA nephropathy are significantly higher than that in sera from patients with other glomerular diseases and healthy adults. These findings suggest that an increase in serum IgA from patients with IgA nephropathy may be mainly due to an increase in polymers rather than the monomer of IgA.

Chromatography, Gel↗