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Biomedical subjects

M Endo

Publications and source records attributed to M Endo.

At least 721 records · Page 40Linked to original sources
PubMed1990

[Iatrogenic left main coronary artery stenosis following aortic valve replacement: two cases report].

Two cases of iatrogenic left main coronary artery stenosis (ICOS) following aortic valve replacement (AVR) are documented. The first patient began to have chest pain due to myocardial ischemia 7 months after AVR. Angiographically, the ostium of her left coronary artery was severely stenotic. She refused coronary bypass grafting. She died of myocardial infarction due to ICOS, 23 months after AVR. Histologically, a loose cellular connective tissue was found around the stenotic coronary arterial lumen. The other patient had chest pain 4 months after aortic and mitral valve replacement. The ICOS was diagnosed angiographically. After she had coronary bypass grafting, she was free from myocardial ischemic symptoms. The onset of myocardial ischemic symptoms in the first six months after AVR is highly suggestive of ICOS, and urgent coronary angiography is recommended and, if proximal stenosis of the coronary arteries is diagnosed, coronary bypass grafting should be performed.

Adult↗
PubMed1990

A case of pseudothrombophlebitis due to inguinal synovial cyst.

A case of pseudothrombophlebitis of the whole right leg by an inguinal synovial cyst that had a compressed femoral vein is presented. Differential diagnosis is important in order to avoid hemorrhagic complications. The usefulness of ultrasonography and necessity of phlebography are emphasized. Radical excision of the cyst is recommended.

Diagnosis, Differential↗
PubMed1990

[Experimental study on myocardial preservation by perfluorochemical "comparison of myocardial preservation effect between continuous perfusion and intermittent perfusion"].

The effect of myocardial preservation with perfluorochemical as cardioplegic solution was studied with isolated canine hearts which was compared between intermittent coronary perfusion and continuous coronary perfusion. Intermittent perfusion group (group I) was infused every 30 minutes during 5 hours ischemia with oxygenated perfluorochemical at the amount of 10 ml/kg. Continuous perfusion group (group II) was infused continuously at the amount of 10 ml/kg/30 minutes. After 5 hours of ischemic time, total perfusion volume of both group were same 100 ml/kg. The comparison of myocardial preservation effect between group I and group II was examined with biochemical study, hemodynamic study and histological study. As a result, biochemical study such as GOT, CPK, and Lactate showed higher in group II than in group I, and value of catecholamine and adenylate levels in myocardial tissue showed higher in group I than in group II. In hemodynamic study, LVSW and LVEDP showed excellent value in group I, but never showed adequate function in group II at late working phase. On the other hand, LVmax dp/dt was recovered excellently in group I but in group II was not recovered at early working phase. In histological findings with electronic microscopy, there were some limited ischemic lesion in group II, which was suggested disturbance of micro circulation. It may be attributable to low perfusion pressure of continuous perfusion method. Finally, with regard to SOD (Super oxide dismutase) consumption, group I took higher than group II, and also oxygen consumption. It shows that in group I there is an effective activity of aerobic metabolism during ischemia, which explain not only the improved functional recovery but also generation of free radical, caused by super oxide etc. It is concluded from these results that intermittent perfusion has provided excellent preservation against myocardial ischemia, and also has possibility of danger to set up reperfusion injury.

Animals↗
PubMed1989

[Magnetic resonance imaging of bladder tumors: superiority of serial "Fast SE" assisted by Gd-DTPA in tumor staging].

Eighteen cases with bladder tumors were examined by means of superconducting MRI. Sequences used were spin echo (TR/TE (msec) = 500/20 as T1WI (weighted image) and 1500/80 as T2WI) and serial "fast spin echo (fast SE)" pre/post Gd-DTPA administration. "Fast SE" was a new technique offering a distinct T1WI (TR/TE = 100/14, utilizing a 14 second breath hold). Slice thickness of "fast SE" was 10 mm and slice plane was selected perpendicular to the tumor base to detect the extent of invasion. Serial scan of "fast SE" was performed before and immediately after 0.1 mmol/kg Gd administration. Scanning was completed before the bladder was opacified by Gd. Tumor and normal mucosa were both markedly enhanced whereas the surrounding muscle layer remained hypointense. On delayed scan, the elevated character of the tumor was outlined by opacified urine but the distinction between the mucosa and the muscle layer became unclear. Total cystectomy (TC) was performed in 6 of 18 cases and pathological tumor extension was correlated with MR findings. Transurethral resection (TUR) was performed in the remaining 12 patients, and the tumor extension was assessed by follow-up biopsy after TUR. Intact liner hypointensity indicated superficial lesions (= less than pT2), while disruption of the linear hypointensity corresponded pathologically to deep muscle invasion (= greater than pT3a). Accuracy of serial "fast SE" in tumor staging was 94% (17/18). Serial "fast SE" allowed the distinction of superficial from invasive tumors more accurately than conventional studies, and therefore assisted in choosing the correct operative method.

Adult↗
PubMed1989

[Effect of various contrast media on platelet aggregation--an in vivo study].

Recently, some radiologists using non-ionic contrast media for angiography have noted the increased tendency of thrombus formation in the injection syringe or angiographic catheter contaminated with blood. In vitro studies by some authors have shown that non-ionic contrast media had only a slight anticoagulative effect as compared with ionic contrast media. But the in vivo studies comparing the anticoagulative effect of both ionic and non-ionic contrast media have not been performed previously. We investigated the effect of non-ionic and ionic contrast media on platelet aggregation in 40 patients undergoing angiography. The in vivo study revealed a negligible influence of both non-ionic and ionic contrast media on systemic platelet aggregation. The dose of contrast media also showed no significant correlation with platelet aggregation. Our study suggests that the data from in vitro experiments cannot be extended to in vivo study as for the relationship between contrast media and their anticoagulant effect.

Adult↗
PubMed1989

The assessment of pelvic circulation after internal iliac arterial reconstruction: a retrospective study of the treatment for vasculogenic impotence and hip claudication.

The standard surgical treatment of vasculogenic impotence or hip claudication involves repairing vascular lesions, especially in the internal iliac arteries. It is difficult, however, to make a definite diagnosis or an accurate judgement of the therapeutic effects of this treatment due to the trouble in ruling out any other disorders. During the last five years, 19 patients with impotence and associated apparent internal iliac artery stenosis or occlusion and 2 patients with hip claudication, underwent internal iliac arterial reconstruction. The patients' ages ranged from 37 to 70 with a mean age of 63.7 and the main procedure performed in all patients was aorto-iliac to femoral bypass grafting, or abdominal aortic aneurysmectomy. A retrospective study revealed that 74 per cent of those treated regained penile erectile activity postoperatively, and that hip claudication disappeared completely in all cases. One of the patients received percutaneous transluminal angioplasty (PTA) for the treatment of vasculogenic impotence, after which his postoperative penile brachial pressure index (PBPI) improved, demonstrating a statistically significant difference compared to the preoperative value. Trans-anal Doppler measurement also proved useful in providing complication-free perioperative understanding of pelvic circulation.

Adult↗
PubMed1989

Cryosurgical ablation of accessory atrioventricular pathways without cardiopulmonary bypass: an epicardial approach for Wolff-Parkinson-White syndrome.

An epicardial approach has been developed and applied to 31 Kent bundles (23 parietal, 1 right anteroseptal, and 7 posteroseptal bundles) in 28 patients with Wolff-Parkinson-White syndrome. Our technique consists of dissection of the atrioventricular fat pad of the Kent bundle site using an ultrasonic aspirator and subsequent cryosurgical ablation. For the left parietal and posteroseptal pathways, the apex of the heart has to be retracted upward, but this maneuver was well tolerated in all patients. Moreover, even through a narrow operative field, the ultrasonic aspirator permitted deliberate dissection on the beating heart without injury to major coronary vessels or the atrial wall. In 89% of the patients, operation was performed without the use of heart-lung bypass. All patients were free from preexcitation. With this technique, all Kent bundles except that adjacent to the atrioventricular node can be ablated on the beating heart, usually without heart-lung bypass.

Adolescent↗
PubMed1989

Quantitative determination of sulfated glycopeptide by two-dimensional electrophoresis on cellulose acetate membrane.

Quantitative determination of the sulfated glycoproteins present in tissue and secretion fluid was performed. After digestion of the specimen with pronase in order to convert glycoproteins to glycopeptides, the sulfated glycopeptides were separated from a mixture of acidic glycans (glycosaminoglycans, sialoglycopeptides and sulfated glycopeptides) by two-dimensional electrophoresis on cellulose acetate membrane [(1986) J. Biochem. Biophys. Methods 12, 239-246]. After staining with alcian blue, the spot of sulfated glycopeptide on the cellulose acetate membrane was cut out, and then only the dye bound to the sulfated glycopeptide was extracted with a 5% cetylpyridinium chloride solution at 100 degrees C for 15 min. The extract was then measured by absorbance at 615 nm using an authentic sulfated glycopeptide as a standard. This method facilitated the determination of sulfated glycopeptides, which were separated from other acidic glycans, within the range 0-25 micrograms.

Animals↗
PubMed1989

The occurrence of a ca-dependent period in the red light-induced late g1 phase of germinating adiantum spores.

The first cell cycle of Adiantum spores, which is induced by red light (R), was studied with regard to its temporal dependence on Pfr (the active form of phytochrome) and Ca(2+). Responses were quantified with increases of the spore content of DNA, thus limiting the investigation to the reactions taking place before the S phase of the cell cycle (i.e. during G0/G1/S transitions). Spores hydrated for more than 2 days in the standard medium (includes 1 millimolar free Ca(2+)) showed, after stimulation with a saturating R pulse, an increase of DNA beginning at about 25 hours and saturating at about 55 hours. Reversal by far-red light of the inductive effect of R was used to examine the temporal requirement for Pfr. Spores became dependent on the supply of external Ca(2+) when incubated in a low Ca medium (32 nanomolar free Ca(2+) with 10 millimolar EGTA); this culture condition was used, after observing that the DNA increase occurs similarly if Ca(2+) is supplied after the R pulse, to examine the temporal requirement for external Ca(2+). It was concluded that the G1 phase of the spore is separated into three subphases: (a) the Pfr-dependent period which immediately follows the R pulse and varies among individual spores from a few to about 25 hours, (b) the Ca(2+)-dependent period (about 10 hours) which occurs immediately before the S phase, and (c) a gap (15-20 hours) between the two periods. In the Ca(2+)-dependent period, spores require the presence of extracellular Ca(2+). This period occurs only during a determined time after the R pulse, and the competence of spores to enter the S phase is lost sharply if external Ca(2+) is not available continuously during this period.

Journal Article↗
PubMed1989

Ca-induced Ca release in malignant hyperthermia-susceptible pig skeletal muscle.

To provide information regarding the cause of the muscle rigidity in malignant hyperthermia-susceptible (MHS) pigs, the Ca-induced Ca-release mechanism of the sarcoplasmic reticulum (SR), the Ca uptake by the SR, and the Ca-activated tension production of the contractile system were examined in skinned skeletal muscle fibers from MHS and normal pigs. In muscles of MHS pigs, the rate of Ca-induced Ca release was significantly higher than in normal muscle. The potentiation effect on Ca-induced Ca release by halothane and caffeine did not differ appreciably between MHS and normal fibers. The rate of Ca uptake by the SR and the Ca sensitivity of the contractile system of MHS fibers were not different from those of normal fibers, and halothane in an anesthetic concentration exerted no effect on them. Dantrolene inhibited the Ca-induced Ca release at 38 degrees C. These results suggest that the principal cause of malignant hyperthermia (MH) in MHS pigs is due to the enhancement of the Ca-induced Ca-release mechanism of the SR of the skeletal muscle.

Adenosine Triphosphate↗
PubMed1989

Sleep spindles in twins.

Sleep spindle characteristics and spindle power periodicity were studied in 4 identical and 3 fraternal twin pairs (mean age, 16 years). There were no significant genetic effects such as concordance between the identical twins and discordance between the fraternal twins for mean duration, mean amplitude and mean frequency of spindles. Spindle periodicity, which is correspondent to the sleep cycle, was visually more similar between the identical twins than between the fraternal twins. These observations suggest that the sleep cycle which is expressed by periodic appearance of spindle powers is genetically determined. On the other hand, some spindle characteristics and some physical measures had significant relationships. These relationships may suggest that some spindle characteristics are influenced by the individual development rather than by a genetic trait.

Adolescent↗
PubMed1989

[Mechanisms and their pharmacology of mobilization of calcium ion in muscle cells].

Mechanisms and their pharmacology of Ca ion mobilization in skeletal, cardiac and smooth muscles are reviewed. In skeletal muscle, it is very likely that depolarization of T-tubule membrane causes conformational changes of dihydropyridine (DHP) receptors in the T-membrane, which in turn, most probably through some kind of protein-protein interaction, open the Ca2+ release channel in the sarcoplasmic reticulum (SR), the ryanodine receptor. Both the DHP receptor and ryanodine receptor have already been purified and sequenced, but the nature of the information transduction between these proteins still remains to be solved. Both of these proteins appear to have dual functions: the DHP receptor as a voltage sensor as described above and as a voltage-dependent Ca2+ channel and the ryanodine receptor as a physiological Ca2+ release channel and as a Ca2(+)-induced Ca2+ release (CICR) channel, an abnormality of which is known to cause malignant hyperthermia. In cardiac muscle, Ca2+ influx is essential not as the main Ca2+ source but to release Ca2+ from the SR, probably not through the CICR mechanism in the narrow sense but through a mechanism dependent on both Ca2+ and T-tubule depolarization. Several mechanisms of Ca2+ mobilization are used in smooth muscles and their features, different from those in striated muscles, are briefly reviewed.

Animals↗
PubMed1989

Indications for surgery based on lung biopsy in cases of ventricular septal defect and/or patent ductus arteriosus with severe pulmonary hypertension.

Pathologic obstruction of the proximal lumen and secondary atrophy of the media of the peripheral small pulmonary arteries were absolute operative contraindications in cases of VSD and/or PDA with severe pulmonary hypertension. Such patients who were operated on died with no decrease in pulmonary arterial pressure. The index of pulmonary vascular disease (IPVD), a composite and quantitative evaluation of the severity of pulmonary vascular disease, was introduced to determine the operability of other patients. An IPVD rating of 2.2 in Down's syndrome and 2.1 without the syndrome were regarded as the upper permissible limits for surgical intervention based on results of 23 autopsies and 26 lung biopsies of patients operated on before 1981. Open lung biopsy was performed in 51 patients to determine applicability of our operative indications. Twenty-nine cases were considered operable by our criteria, and 28 underwent surgical correction without operative or late death. Twenty-two cases thought inoperable remain under observation. Comparative analysis of the pathology and preoperative hemodynamic data suggested that lung biopsy should be carried out to determine operability in cases with pulmonary vascular resistance greater than 8 units.m2.

Biopsy↗
PubMed1989

Influence of L-methionine-deprived total parenteral nutrition on the tumor tissue and plasma amino acids fraction and the host metabolism: experimental study with Sato lung carcinoma-bearing rats.

Creation of an amino acid imbalance, particularly curtailment of L-methionine, at the tumor cell level is thought to have a favorable effect on the inhibition of tumor growth. In the present study, we examined the influence of a specially-formulated amino acid mixture, avoid of sulfur-containing amino acids (L-methionine and L-cysteine), on the growth and amino acid fraction of Sato lung carcinoma (SLC) and the host metabolism in SLC-bearing rats. The rats were treated by total parenteral nutrition containing the above amino acid mixture, plus other nutrients (methionine-deprived TPN) for 10 days. Tumor growth began to decrease 4 days after the start of this treatment and the size was significantly less at the end of the treatment than in rats receiving conventional TPN with general purpose Vuj-N type amino acid solution as a protein source. The tumor-to-carcass weight ratio also showed a similar trend. In biochemistry, the albumin level and albumin-to-globulin ratio were significantly lower than in the rats receiving conventional TPN but other parameters such as total protein, glucose, GOT and GPT were not affected by the treatment. In the amino acid fraction of the tumor tissue extraction, both L-methionine and L-tyrosine were decreased and L-serine was increased significantly compared with the control group.

Amino Acids↗
PubMed1989

cDNA cloning of human calpastatin: sequence homology among human, pig, and rabbit calpastatins.

cDNA of human calpastatin, an inhibitor protein specific for calpain (EC 3.4.22.17; Ca2(+)-dependent cysteine proteinase) was isolated by screening of a library prepared from human liver mRNA with pig calpastatin cDNA fragment as a probe. The primary structure of human calpastatin was deduced from the nucleotide sequence of the cDNA and compared with that of pig and rabbit calpastatins already reported. Human calpastatin consisted of 673 amino acid residues and had 78% and 77% identity to pig or rabbit calpastatins, respectively. Human calpastatin had a domain structure with four internally repetitive sequences and one N-terminal non-homologous sequence like the other calpastatins. Human calpastatin had two deletions, 22 and 13 residues long in domain L and domain 1, respectively, compared to pig or rabbit calpastatins.

Amino Acid Sequence↗
PubMed1989

Cranial burr hole for revascularization in moyamoya disease.

Currently, superficial temporal artery-middle cerebral artery (MCA) anastomosis, encephalomyosynangiosis (EMS), and encephalo-duro-arterio-synangiosis are used to treat moyamoya disease and are reported to effectively improve ischemic symptoms. All are methods of reversing the flow of blood from the external carotid artery system into the cortical branches of the MCA. As moyamoya disease advances, these operations alone will predictably not correct the deterioration in blood flow in the territory of the anterior cerebral artery. It was noted in a case of moyamoya disease with intraventricular hemorrhage that a burr hole, made in the frontal region for drainage purposes, induced marked neovascularization. Since then, similar frontal burr holes have been made in five juvenile cases of moyamoya disease; this procedure involved making a burr hole in both frontal bones and incising both the dura and the arachnoid membrane. In two cases a frontal burr hole in both frontal bones and incising both the dura and the arachnoid membrane. In two cases a frontal burr hole was placed simultaneously with EMS, and in the others the frontal burr hole was made following EMS. The clinical symptoms improved after the frontal burr hole was made, and dynamic computerized tomography revealed improved circulation in the frontal regions. Together with conventional surgical therapy for juvenile cases of moyamoya disease, this operation is considered beneficial both to the circulation in the frontal region and for the protection of frontal brain function.

Arterial Occlusive Diseases↗