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Biomedical subjects

M E Ward

Publications and source records attributed to M E Ward.

174 records · Page 10Linked to original sources
PubMed1972

Human antibody response to lipopolysaccharides from Neisseria gonorrhoeae.

Red cells coated with lipopolysaccharides from three different strains of Neisseria gonorhoeae have been used as antigens in a haemagglutination test for gonococcal antibodies. For each strain the geometric mean titre in sera from 50 male and 25 female patients was significantly higher than that in 50 normal controls. The most useful smooth strain, G1, picked out 84% of females and 46% of males from a group of patients known to have gonorrhoea, but only gave 2% positives among controls. The rough strain, G2, gave 10% positives in controls and 31% in patients. The results suggest that the method is worth developing further as a diagnostic test and that strain differences are important. False positives were probably due to cross-reacting antibodies.

Antibody Formation↗
PubMed1971

The preservation of gonococci in liquid nitrogen.

Gonococci suspended in 1% proteose peptone containing 8% glycerol can be snap frozen in liquid nitrogen without detectable loss on thawing. This recovery rate has allowed the use of frozen organisms as the starting inoculum for the bulk growth of gonococci and to preserve gonococci both in urethral pus and after primary subculture for use in studies on gonococcal virulence. The method described for freezing urethral pus on charcoal swabs should make it possible to transport infected specimens from areas lacking adequate laboratory facilities.

Charcoal↗
PubMed1970

Nature and Heterogeneity of the Antigens of Neisseria gonorrhoeae Involved in the Serum Bactericidal Reaction.

Sixty strains of Neisseria gonorrhoeae have been classified into four main groups according to their resistance to killing by human complement together with either normal human or immune rabbit antibodies. The rabbit antisera had been raised against 10 of the strains tested. The normal human antibodies had probably been formed against cross-reacting organisms since they could be removed by absorption with N. pharyngis sicca, N. pharyngis flavus, N. catarrhalis, or Escherichia coli. Bactericidal antibodies could be absorbed from both normal and immune sera by N. gonorrhoeae which had been autoclaved or trypsinized, and by red cells coated with gonococcal lipopolysaccharides. The results suggest that the antigens involved in the bactericidal reaction are lipopolysaccharides of several distinct specificities. Since individual sera always reacted more widely in hemagglutination than in bactericidal tests, it is postulated that surface-blocking antigens may restrict access to the lipopolysaccharides in the intact organisms.

Journal Article↗
PubMedDate not supplied

Enhancement of aortic contractility by endothelin following prolonged hypoxia in vivo.

The purpose of this study was to determine if endothelin-1 (ET-1) mediates endothelium-dependent enhancement of rat aortic contractility following exposure to hypoxia. Rats breathed room air or 10% oxygen for 12 or 48 h. Thoracic aortas and plasma were analysed for ET-1 content by radioimmunassay. Aortic rings were mounted in organ bath myographs for measurement of isometric tension during activation by phenylephrine (10(-9)-10(-4) M), in the presence and absence of BQ-123. In some rings, the endothelium was removed. Plasma ET-1 levels were 0.79+/-0.09 pg/ml, 2.00+/-0.36 and 1.88+/-0.21 pg/ml, in normoxic rats and rats exposed to hypoxia for 12 or 48 h respectively (P<0.001, 12 or 48 h vs. control). Aortic ET-1 concentrations were 202.3+/-20.8 fg/mg in normoxic rats, compared to 274.9+/-40.6 fg/mg and 292.4+/-24.4 fg/mg in rats exposed to hypoxia for 12 and 48 h, respectively (P<0.01, 12 or 48 h vs. control) and 155.0+/-43.1 fg/mg in de-endothelialized aortas from rats exposed to hypoxia for 48 h (P>0.05 vs. normoxic controls). Maximum tension during phenylephrine-induced contraction was 0.46+/-0.04 mg/g and 0.33+/-0.03 mg/g in endothelialized rings from rats exposed to hypoxia for 48 h in the presence and absence of BQ-123, respectively (P<0.05 for difference), and 0.28+/-0.07 mg/g in rings in which the endothelium had been removed. Local endothelin release is an important mechanism by which the responsiveness of the systemic vasculature to agonists may be preserved during hypoxia.

Animals↗
PubMedDate not supplied

Ventilatory effects of the interaction between phrenic and limb muscle afferents.

We studied the effects on ventilation and ventilatory muscle activation of stimulation of the central ends of the left phrenic and gastrocnemius nerves separately and concurrently in 10 spontaneously breathing, alpha-chloralose anaesthetized dogs. The nerves were stimulated for 1 min, at a frequency of 40 Hz and pulse duration of 1 ms. The phrenic nerve was stimulated at 20 and 40 times twitch threshold (TT). During these stimulation periods ventilation increased by 39% and 79% of control values, respectively. The gastrocnemius nerve was stimulated at 20 times TT. This produced a 90% increase in ventilation. Stimulation of either nerve resulted in increases in the activity of the right diaphragm, parasternal intercostal and alae nasi muscles comparable in magnitude to the increase in tidal volume. The activities of the genioglossus and transversus abdominis muscle increased to a much greater extent than did the other muscles under all conditions. In contrast, triangularis sterni activity remained unchanged during stimulation of either nerve. The phrenic nerve was then stimulated at 40 times TT for 1 min with superimposed gastrocnemius nerve stimulation (20 times TT) during the last 30 s. Ventilation had risen by 66% after 30 s of phrenic nerve stimulation. With the addition of gastrocnemius nerve stimulation, ventilation rose by a further 84% for a total increase of 150% of the control value. Mathematical summation of the responses to individual nerve stimulation at these intensities predicted a 156% increase in ventilation. Similar degrees of summation were found with respect to respiratory muscle activation. We conclude that the interaction between phrenic and limb muscle (gastrocnemius) afferent is additive with respect to their effects on ventilation.

Animals↗
PubMedDate not supplied

Evaluation of intravenous tramadol for use in the prehospital situation by ambulance paramedics.

INTRODUCTION: An evaluation of the opioid analgesic tramadol (Zydol, Searle United Kingdom) was carried-out by ambulance paramedics to assess its efficacy in providing pain relief in the prehospital situation. METHOD: Type of subjects--Patients suffering severe pain from any cause uncontrolled by other simple methods. Number of subjects--101 patients received tramadol and 41 patients served as a control. Study design--Random, open study. Statistical tests--Pain score at scene and on arrival at hospital were compared using Fisher's exact tests (2 sides). Logistic regression analyses also were applied to other factors. RESULTS: Pain scores improved for 93.1% of the tramadol treated patients and for 44.0% of the controls. A total of 30.7% of patients treated with tramadol complained of nausea after treatment compared with 17.1% before treatment and with 12.2% of the control patients. CONCLUSIONS: Pain was significantly decreased by the administration of tramadol. It was safe with only minimal side effects, the major one being nausea. Suggestions are made for areas of further study.

Adult↗