Search PubMed⌕ Search

Biomedical subjects

M E Rose

Publications and source records attributed to M E Rose.

At least 91 records · Page 5Linked to original sources
PubMed1983

Antibodies to coccidia: detection by the enzyme-linked immunosorbent assay (ELISA).

The ELISA test was used for the detection of antibodies to coccidia in the serum and/or egg yolk of chickens infected with Eimeria acervulina, E. maxima or E. tenella and in the serum of rats infected with E. nieschulzi. Antigens prepared from different developmental stages of the parasite were tested and the cross-reaction between different species of Eimeria were examined. The variability in cross-reactivity of different species and the advantages and possible applications of the test are discussed.

Animals↗
PubMed1982

Coccidiosis: T-lymphocyte-dependent effects of infection with Eimeria nieschulzi in rats.

The intestinal pathology caused by infection with Eimeria nieschulzi was investigated and comparisons were made between the effects in athymic nude (rnu/rnu) rats and their heterozygous (rnu/+) litter-mates. Most of the changes noted, i.e. increase in gut weight, partial villous atrophy and increased numbers of mast, goblet and pyroninophilic cells were shown to be largely or wholely thymus dependent. The numbers of intraepithelial lymphocytes were decreased in both groups during the period of study. The peripheral blood leucocyte response was similar in both groups of rats during a primary infection but differed after a challenge inoculum, indicating that the secondary type of response which occurred in the rnu/+ rats was thymus dependent, as is resistance to reinfection.

Animals↗
PubMed1982

Immunity to coccidia in chickens: adoptive transfer with peripheral blood lymphocytes and spleen cells.

Suspensions of cells prepared from the caecal tonsils and spleen, and the peripheral blood lymphocytes of chickens immune to Eimeria maxima, were tested for their ability to transfer resistance to syngeneic recipients. The intravenous injection of approximately 6 x 10(8) spleen cells or peripheral blood lymphocytes caused a significant reduction of oocyst production by the challenged recipients, in comparison with controls which were uninjected or given cells from birds susceptible to E. maxima. Peripheral blood lymphocytes appeared to be most effective when obtained 10-15 days after a primary, or 3-10 days after a secondary inoculation of oocysts. The peripheral blood lymphocytes which participate in the early response to challenge of immune birds were not found to be protective. When given intraperitoneally, greater numbers of spleen cells were required to reduce oocyst production, and small numbers of caecal tonsil cells were ineffective. The ability of the various cell suspensions to transfer antibody and cell-mediated responses was monitored with 'marker' antigens. There was some indication that both types of response were involved in protection.

Animals↗
PubMed1982

The biotransformation of 17 alpha-ethynyl[3H]estradiol in the rat: irreversible binding and biliary metabolites.

1. The irreversible binding of metabolites of 17 alpha-ethynyl[6,7-3H]estradiol ([3H]EE2) to intracellular proteins, and the biliary metabolites of [3H]EE2, were studied in male rats. 2. Very low levels of irreversible binding to hepatic microsomal and soluble proteins were observed. 3. Approx. 75% of the radiolabelled material excreted in bile was present as beta-glucuronides and arylsulphate esters. 4. The compounds liberated from the biliary conjugates by enzymic hydrolysis consisted of EE2, 2-hydroxy-EE2, 16-hydroxy-EE2, 2-methoxy-EE2, 2-hydroxymestranol and at least three additional metabolites not fully identified. 2-Methoxy-EE2 was the principal metabolite. 5. EE2 and all its identified metabolites were excreted as both beta-glucuronides and arylsulphate esters. The glucuronide fraction contained a greater proportion of EE2, a lower proportion of 2-methoxy-EE2 and a lower ratio of 2-methoxy-EE2 to 2-hydroxymestranol than the arylsulphate fraction.

Animals↗
PubMed1982

Mucosal transport of Eimeria tenella in the cecum of the chicken.

A study of the early life history of Eimeria tenella with the electron microscope confirmed that sporozoites do not directly enter the enterocytes of the crypts, in which they develop, but are carried there by host cells. However, these cells are not macrophages, as previously thought, but intraepithelial lymphocytes. The evidence presented demonstrates that sporozoites first penetrate surface enterocytes and then enter intraepithelial lymphocytes that leave the epithelium, pass through the lamina propria and enter the crypts.

Animals↗
PubMed1981

The origin of IgA in chicken bile: its rapid active transport from blood.

In the chicken, there are two bile ducts, one draining the left lobe of the liver directly into the duodenum and the other draining the right lobe via the gall bladder. Cannulation of these ducts enabled us to collect bile in unanesthetized birds and to compare the IgA concentrations of the hepatic bile, gall bladder bile and blood serum. Bile from the cystic and hepatic ducts of the same bird contained similar amounts of IgA (1.7 mg/ml), roughly 10 times as much as in serum, but considerably less than that found in the concentrated bile collected by aspiration from the gall bladder (8 mg/ml). Ligation of the two bile ducts resulted in a three to fourfold increase in the concentration of IgA in serum suggesting that IgA is normally removed from serum by the biliary route. This was confirmed by a substantial recovery of i.v. injected, radiolabeled monoclonal dimeric human IgA in the bile corresponding to a rapid active clearance from the blood circulation; negligible amounts of monomeric human IgA, similarly injected, were recovered from the bile.

Animals↗
PubMed1981

Presence of a complete endothelial barrier between lymph and lymphoid tissue in the lumbar lymph nodes of the duck (Anas platyrhynchos).

The ultrastructure of the lumbar lymph nodes of the domestic duck is described and compared with published accounts of mammalian lymph nodes. The barrier to cell migration between lymphoid tissue and lymph is more formidable in the duck than in the mammal. In the duck the lymphatic endothelium lining the lymph spaces is continuous and bonded with desmosomes whereas in the mammal it is unbonded and fenestrated. In both types of node there are filtering zones and areas of dense lymphatic tissue but their topographical organisation differs. There is no cortex or medulla in the duck lymph node and filtration occurs in extensive lymphatic spaces traversed by numerous trabeculae to which are attached rounded phagocytic macrophages. Dense lymphoid tissue is scattered throughout the node, suspended by trabeculae or attached to the capsule and there are blood vessels analogous to the post capillary venules of mammalian nodes where lymphocytes migrate between blood and lymphoid tissue.

Animals↗
PubMed1980

Utilization of a glycol-stabilized liquid NAD for the measurement of three enzymes on the GEMSAEC.

Some analytical parameters have been investigated for a recently described stabilized liquid coenzyme technology in which water-free NAD is dissolved in 1,2 propanediol. Correlation for 108 specimens assayed for AST, ALT and LD with a reference method in which glycol-based NAD was absent was greater than or equal to 0.998 with near identical reproducibility over a period of at least 107 days. Mean recovery of exogenous serum enzymes in this linear kinetic assay is 103%. With the option of mixing only the volume of reagent needed for the enzymatic assay, waste can be eliminated as compared to more costly preparations stabilized by lyophilization. Hazards from an impure water supply are avoided since no reconstituting volume is required.

Alanine Transaminase↗
PubMed1980

Coccidiosis: localization of lymphoblasts in the infected small intestine.

Peripheral blood lymphocytes of chickens and thoracic duct lymphocytes and mesenteric lymph node cells of rats, were labelled with 125I-deoxyuridine and injected intravenously into chickens or rats respectively. Sixteen to 18 h later the intestines of coccidia infected animals contained more radioactivity than those of uninfected controls. This result was obtained with cell suspensions from both infected and normal donors indicating that, as with nematode infected rodents, the increased homing of the cells to parasitised gut was not antigen specific. In chickens the stimulus which causes the increased homing of cells to the intestine was induced within hours of parasite inoculation. This reflects the rapid response of this host, previously described with other parameters, and which may be characteristic of birds.

Animals↗
PubMed1980

Basophils and eosinophils in three strains of rats and in athymic (nude) rats following infection with the nematodes Nippostrongylus brasiliensis or Trichinella spiralis.

A previous report showed that infection with the nematode Nippostrongylus brasiliensis stimulates a basophilia as well as an eosinophilia in the blood of August rats. The present study shows that blood levels of basophils and eosinophils were increased in two other rat strains, one inbred and one outbred, after infection with N. brasiliensis, and infection of two inbred rat strains with Trichinella spiralis also stimulated a basophilia as well as an eosinophilia. No increase occurred in basophils or eosinophils in athymic (nude) rats infected with N. brasiliensis, although both these cell types were found in the blood of control, specific pathogen free, nude rats in numbers comparable to those in specific pathogen free, heterozygote controls of the same strain. Rat basophils usually have few granules and in blood smears often appear as if they were partly degranulated. Basophils from uninfected nude rats contained more negative than positive staining granules compared with basophils from parasitized heterozygotes. The possession of small numbers of granules which vary in their reaction to stains of the Romanowski type is a normal feature of rat basophils in blood smears. Consequently rat basophils differ in these respects from those of other species.

Animals↗
PubMed1979

Failure of nude (athymic) rats to become resistant to reinfection with the intestinal coccidian parasite Eimeria nieschulzi or the nematode Nippostrongylus brasiliensis.

The course of each of three successive infections with Eimeria nieschulzi in nude (athymic) rats was the same as the primary infection in nu/+ animals, with the production of more oocysts. This indicates that resistance to reinfection with this parasite is mediated by T lymphocytes but that these cells do not control the duration of the life cycle, since oocyst production was not prolonged in the nu/nu rats. After the three infections with E. nieschulzi, the rats were exposed twice to the intestinal nematode Nippostrongylus brasiliensis, and the nu/nu were completely susceptible even to the second infection. Egg production by both infections in the nu/nu animals was similar and continued at a high plateau level for 28 days before falling to a low level. It appears that the strain of N. brasiliensis used in this study is unable to sustain high egg production for more than 4 weeks in T cell deficient rats.

Animals↗
PubMed1979

Immunity to coccidiosis: T-lymphocyte- or B-lymphocyte-deficient animals.

Comparisons were made between infections with Eimeria spp. in normal animals and in animals with functional deficiencies in either T-lymphocytes (athymic nude rats) or B-lymphocytes (bursectomized chickens). Approximately three times more oocysts of E. nieschulzi were passed during a primary infection in the nu/nu rats, and in contrast to the nu/+ rats, they were completely susceptible to reinfection. Nu/nu rats did not produce agglutinating antibodies to sporozoites, and injections of serum from immunized nu/+ rats caused a reduction of oocyst production during a primary infection in both nu/nu and nu/+ rats. In chickens, oocyst production in primary infections with E. maxima or with E. acervulina was increased 1.5 to 2-fold in the bursectomised (BX) groups, and the clinical effects of infection with E. maxima were greater than in controls. The BX birds were slightly more susceptible than the controls to challenge inoculations of oocysts, but nevertheless, they were very substantially immune. Tests for the functioning of B- and T-lymphocytes indicated that in the BX birds there was a severe deficiency of B-cell function, but near-normal T-cell function. The results show that T-lymphocytes are essential for immunity and that, although they may function partly as helper cells for immunoglobulin production, their major effect is exerted via some other mechanism.

Agglutinins↗