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Biomedical subjects

M E Hodes

Publications and source records attributed to M E Hodes.

At least 109 records · Page 6Linked to original sources
PubMed1983

Immunohistochemical demonstration of altered functional differentiation of pancreatic tissue in sacrococcygeal teratomas.

The finding of teratomas with mature-appearing pancreatic tissue showing abnormal production of immunoreactive amylase appears to contradict earlier suggestions that somatic tissue in teratomas that appears mature also functions normally. It therefore seems judicious to study carefully both morphologic and functional characteristics of tissues in teratomas before utilizing them as experimental models of tissue differentiation, since at least in the case of human amylase, functional differentiation does not always follow morphologic differentiation.

Amylases↗
PubMed1983

Immunohistochemical demonstration of ribonuclease and amylase in normal and neoplastic parotid glands.

Antibodies specific for bovine ribonuclease A (antiRNase A) were raised in rabbits, and immunologic cross-reactivity between bovine RNase A and human salivary gland RNase was demonstrated. The antiRNase A served as the primary antibody in the peroxidase-antiperoxidase immunohistochemical technique. Paraffin blocks of five normal human parotids and 20 parotid tumors were examined. In normal parotid and in cases of cystadenoma lymphomatosum, immunoreactive RNase was localized in the ductal epithelium, evidence of the ductal cell origin of these benign tumors. RNase immunoreactivity was noted in the adenomatous structures and in cells isolated in the myxoid matrix of pleomorphic adenomas, which supports recent evidence of an epithelial origin of these tumors. Malignant acinar cells of acinic cell carcinoma were strongly positive for immunoreactive RNase, while acinar cells of normal parotid were uniformly negative. This expression of the gene for RNase A probably represents a loss of differentiation (i.e., control) of the neoplastic acinar cells. Further evidence for this hypothesis was obtained by treating these tumors with an antihuman salivary amylase antibody, which is localized in normal acinar cells. No immunoreactive amylase was observed. The results support the idea that immunoreactivity need not accompany enzyme activity, as the presence of immunoreactive RNase was noted in all neoplastic tissues examined. Immunohistochemical localization of two antigens in the same tissue demonstrates the varied biochemical changes associated with parotid neoplasia.

Adenoma↗
PubMed1982

The genetic analysis of monilethrix in a large inbred kindred.

The gene for monilethrix was segregating in a large inbred kindred. Pedigree analysis reaffirms an autosomal dominant mode of inheritance. Expressivity appears equally variable within and between sibships while penetrance, in contrast to previous studies, seems to be complete.

Consanguinity↗
PubMed1982

The dermatoglyphic and clinical features of the 9p trisomy and partial 9p monosomy syndromes.

The physical and dermatoglyphic features obtained from published reports of 128 patients with the trisomy 9p syndrome and 27 patients with the partial 9p monosomy syndrome are tabulated. This information is also provided on two new individuals with each of these chromosomal disorders. The dermal ridge patterns and palmar creases of trisomy 9p which are most helpful from a diagnostic standpoint are zygodactylous or absent palmar digital triradii, brachymesophalangy, reduced total finger ridge count, complex thenar/ID I patterns, transverse palmar ridge alignment, simian creases, distal axial triradii, and great toe and hallucal arch patterns. The characteristic features in partial 9p monosomy include dolichomesophalangy with accessory finger flexion creases, digital whorl patterns and elevated total finger ridge count, distal axial triradii, simian creases, and palmar dermal ridge dissociation.

Aneuploidy↗
PubMed1981

Correction of the anomalous electrophoretic behavior of ribonuclease A.

Ribonuclease A behaves anomalously on polyacrylamide gel electrophoresis at acid pH. The distance traveled by the protein is a function of the amount of enzyme added at the lower range of detectable activity (10 pg to 100 ng). Addition of myoglobin (1 mg/ml) abolishes the anomaly. The observations are consistent with the known affinity of RNase for anions. Caution is warranted in the interpretation of apparent electrophoretic variants of RNase observed at low concentrations of enzyme.

Animals↗
PubMed1981

Isozymes of ribonuclease in human serum and urine. I. Methodology and a survey of a control population.

Methods are presented for the electrophoretic analysis of ribonuclease (RNase) enzymes in human serum and urine. Protocols for sample treatment, electrophoresis, and the RNase zymogram technique are described. With the application of these methods, RNase from serum and urine was separated into components differing on the basis of charge (charge isomers or "isozymes"), but not differing with respect to hydrolyzable sialic acid residues. Preliminary characterization of the electrophoretically separated components showed that some of the RNase species have different properties (pH optima and substrate preference). The major urine RNase isozymes appeared to be distinct from the major serum RNase isozymes. A survey of a control population indicated that the major serum and urine RNase enzymes are not genetically polymorphic.

Electrophoresis, Polyacrylamide Gel↗
PubMed1981

Isozymes of ribonuclease in human serum and urine. II. A survey of patients with cystic fibrosis.

A comparison of serum and urine RNase isozymes from CF patients, heterozygotes, and control subjects demonstrated that the major RNase isozymes are not abnormal in CF. Some preliminary evidence indicated that RNase activity of the pancreatic extracts administered to CF patients may appear in the urine and result in a slightly different urine isozyme pattern. In addition, the mean serum RNase activities of the CF patients and heterozygotes were significantly greater than the mean activity of the controls, but there was much overlap in values between the groups.

Animals↗
PubMed1981

Study of two cases of ring 13 chromosome using high-resolution banding.

The chromosomes of two patients with ring 13 (r13) were studied using high-resolution RBG banding of prometaphase cells. The rings of the two patients differ slightly in breakpoints. Cell with multiple single, double-sized rings, quadruple-sized rings, rod- and ring-shaped fragments, and fragments showing varied states of condensation were seen, as were cells monosomic for chromosome 13. The evolution of these cell lines as a result of sister chromatid exchange, nondisjunction, ring breakage, and premature chromosome condensation is discussed. Clinical features of these patients reflect the heterogeneity of phenotype for r13 patients. Each case includes a feature of trisomy 13. The significance of mosaicism of cell lines in patients bearing ring chromosomes is considered with respect to variation in clinical findings.

Abnormalities, Multiple↗