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Biomedical subjects

M E Boon

Publications and source records attributed to M E Boon.

At least 127 records · Page 7Linked to original sources
PubMed1986

The application of a microwave technique for the preparation of cell blocks from sputum.

Microwaves stimulate diffusion in tissue and affect the proteins and membrane of the cell. These actions can be used to shorten the time required to fix and prepare cell blocks from sputum. The fixative containing ethyl alcohol and polyethylene glycol is particularly suitable for microwave enhanced fixation. Applying a commercially available domestic microwave oven, in which the temperature and power level can be set, cell blocks can be prepared from fresh sputum in 35 min. The microscopical results are of a superior quality because of the microwave-induced homogeneous distribution of the effects of the applied immersion fluids on the cells. The method is very successful for all specimens containing a large amount of mucous. Health hazards for laboratory personnel are nil in this cell blocking method; in contrast to when fresh sputum containing infectious agents is used for smear preparation.

Carcinoma, Squamous Cell↗
PubMed1986

Histochemistry of puberty. A multidisciplinary study in the rat.

A displacement of structures in the hypothalamic region of the rat has been reported in previous papers. 1 micron granules located in neuroblast like cells containing catalase activity (which were detected by enzyme histochemical methods) undergo a shift from the median eminence (at day 22 after birth) towards the ventromedial part of the hypothalamus (at day 35 after birth). This displacement terminates in the medio-basal part of the arcuate nucleus (at day 55 after birth). A similar displacement of dopaminergic fluorescent perikarya has been described following the same route, with the same time sequence. Neonatal application of monosodium glutamate (MSG) leads to an acceleration of the displacement of both the catecholaminergic and the catalase containing perikarya. However after day 25 (after birth) all fluorescent perikarya disappear whereas the catalase containing cell survive when they finally reach the arcuate area.

Aging↗
PubMed1986

Masking effect of hormonal contraceptives on discriminating quantitative features of visually normal intermediate cells in positive and negative cervical smears.

In quantitative studies of visually normal intermediate cells in smears from patients with cervical neoplasia, the contraceptive status of the patients has not previously been taken into account. In this study cervical smears from 151 patients with cervical intraepithelial neoplasia (CIN) III or invasive carcinoma and from 360 normal controls were grouped according to week of menstrual or pill cycle and mode of hormonal contraception. The nuclear-cytoplasmic (N/C) ratio of visually normal intermediate cells in smears from patients with neoplasia was significantly different from that of the normal controls (P less than .001). Based on nuclear area and N/C ratio, the percentages of intermediate cells correctly classified as having come from positive or negative smears were significantly better in women with ovulatory cycles (non-users) than in women using hormonal contraceptives (P less than .025). It is concluded that hormonal contraceptives can mask the salient quantitative features of visually normal intermediate cells from patients with CIN and the contraceptive status thus has to be taken into account in such studies.

Carcinoma↗
PubMed1985

Fluctuations in quantitative features of intermediate cells in normal cervical smears during the menstrual cycle of ovulating women and contraceptive users.

In this study we report on the variation in nuclear and cytoplasmic size of intermediate cells in normal cervical smears, in relationship to the week of the menstrual cycle and in relationship to the mode of contraception. A total of 18000 cells from 360 different women was studied. A significant difference in nuclear size of intermediate cells in smears from ovulating women not using contraception was found in comparison with intermediate cells in smears from women using contraceptive pills (ANOVA: F(1.312) = 4.98, p less than 0.02). Also a significant difference in the nuclear size of intermediate cells in smears from women using norgestrel- (or levonorgestrel)-containing formulas compared with lynestrenol-containing formulas was found (ANOVA: F(1.232) = 5.82, p less than 0.01). The influence of exogenous and endogenous hormones on cell populations in cervical smears is discussed. It is concluded that in studies on intermediate cells in cervical smears the contraceptive status of the women has to be taken into account.

Adult↗
PubMed1985

Koilocytotic lesions of the cervix: the interrelation of morphometric features, the presence of papilloma-virus antigens, and the degree of koilocytosis.

We studied the interrelation of morphometric features, the presence of human papilloma virus antigens (localized by the immunoperoxidase method), and the degree of koilocytosis in koilocytotic cervical intraepithelial neoplasia. We determined the morphometric features of the cells from the deep, the middle, and the superficial layers of the affected koilocytotic epithelium and in non-koilocytotic immature metaplasia and squamous cervical epithelium. This approach allows quantification of disturbances of maturation in cervical epithelium. Our quantitative findings support the contention of other authors that human papilloma virus infection is associated with a morphologically distinct lesion, which forms a morphological continuum with neoplasia. It can be argued that, in addition to the degree of koilocytosis, nuclear enlargement and excessive cellular enlargement in the middle layer of the affected epithelium are viral-related effects. With increasing immaturity of the cervical intraepithelial neoplasia all investigated viral-related changes are less prominent. These findings suggest that in neoplastic transformation the morphological and antigen expression of human papilloma virus infection is suppressed.

Antigens, Viral↗
PubMed1984

Classifying breast carcinomas with DNA measurements and morphometry.

The relation between the DNA distribution pattern and the variables DNA content and nuclear area was investigated in 73 breast carcinomas. According to the DNA patterns, the tumors were subclassified into groups I, II, III, and IV (Auer's classification). The Auer's classes correlate with prognosis. In this investigation it was shown that Auer's classes strongly correlate with the standard deviation of DNA content and mean nuclear area. The carcinomas were subclassified using data of DNA content and nuclear area. For the establishment of this subclassification, multivariate analysis was applied. It was evident that Auer's group III was a hybrid group. Therefore a second classification rule was calculated without the data of Auer's group III. This second classification rule was even more successful in determining the Auer's group of each individual case. Our investigation shows that it is possible to subclassify breast carcinomas objectively using DNA content and nuclear area of the tumor cells.

Breast Neoplasms↗
PubMed1984

Prognostic scoring using cytomorphometry and lymph node status of patients with breast carcinoma.

Applying morphometry on FNA smears may lead to meaningful prognostic subgrouping of breast carcinomas. A statistical analysis of the cytomorphometric and clinical data of 73 patients with breast carcinoma was performed. By multivariate analysis, taking into account various prognostic factors, it was shown that variations in nuclear area and the presence of axillary metastases were the most important prognosticators. By weighing these factors a prognostic score was obtained. According to that score the patients were classified into 4 score groups: the decreasing survival with increasing score was evident. The prognostic score was also related to the metastasis-free period. This prognostic scoring is relatively easy to perform, and can be done in routine pathology laboratories.

Aged↗
PubMed1984

Localizing chlamydial infection in cervical biopsies with the immunoperoxidase technique.

One hundred and two cervical biopsy specimens containing varying degrees of chronic inflammation were stained for chlamydial antigens with the immunoperoxidase technique. Seven cases (6.9%) were positive. Histologically, six (84%) of the Chlamydia-positive cases contained severe chronic inflammation, all contained reparative atypia, and two (28%), follicular cervicitis. When evaluated separately, 22% (six of 27) of the specimens with severe inflammation were positive in contrast to 0% (0 of 45) of cases with mild inflammation. Positively staining cells were located primarily in columnar epithelium and reparative atypia and occasionally in areas of immature squamous metaplasia. The cytological finding which correlated with positive staining was cytoplasmic vacuolation; however, cytoplasmic vacuoles were common in cells which did not stain positively, and it was impossible to predict on histological grounds which cells/specimens would stain positively by immunoperoxidase. Because of these findings, the presence of chlamydial infection should be strongly suspected whenever the cervical biopsy specimen contains severe inflammation and repair. Although tissue staining may not be as sensitive as culture for diagnostic purposes, it can be performed rapidly and simply and may be a useful special stain in cases where the diagnosis of chlamydial infection is not suspected clinically or cultures are not immediately available.

Biopsy↗
PubMed1984

Analysis of number, size and distribution patterns of lipid vacuoles in benign and malignant mesothelial cells.

A quantitative analysis of the lipid vacuoles in benign hypertrophic and neoplastic mesothelial cells, using a size-independent distribution index, showed that computer-assisted image analysis for distribution patterns of cytoplasmic components can aid in distinguishing benign from malignant cells. Benign mesothelial cells had fewer lipid vacuoles, which were smaller and predominantly found around the nuclei. It is argued that, due to the high surface tension in the lipid vacuoles, the largest vacuoles are found in the center of the cells, which is the least flattened part of the air-dried mesothelial cells. It seems likely that the distribution pattern of rigid substructures, such as lipid vacuoles, varies between histologic and cytologic material as well as between cells processed by different cytologic methods with various cell-flattening artifacts. The study of the distribution of cytoplasmic components that differ in size was enhanced by using the defined size-independent distribution index, which incorporates the radius of the cell, the radius of the vacuoles and their respective centers of gravity.

Humans↗
PubMed1983

Cervical cytology and Chlamydia trachomatis infection.

We found, as have others, a strong correlation between indirect immunofluorescence techniques and cell culture for the diagnosis of Chlamydia trachomatis infection in material obtained from the cervix. Five epithelial cell types indicative of Chlamydia trachomatis infection (indicator cells) were found in smears in patients with positive immunofluorescence. An attempt to diagnose Chlamydia trachomatis infection in 50 routine smears based on the presence of these indicator cells showed false positives and false negatives so that the diagnosis of Chlamydia trachomatis still requires confirmation by immunofluorescence or culture techniques. The indicator cells frequently had the same morphometric features as cells seen with cervical intraepithelial neoplasia, which may explain why some smears revert to normal after patients are given antibiotics.

Chlamydia Infections↗
PubMed1983

Analysis of the performance of pathologists in the grading of bladder tumors.

Fifty-seven transurethrally resected bladder tumors were analyzed to determine whether different pathologists, using the World Health Organization grading system, graded the same bladder tumor differently (interindividual consistency) and whether the same pathologist graded a bladder tumor differently at different times (intraindividual consistency). Disturbingly high inter- and intraindividual inconsistency in the grading of bladder tumors was found. All pathologists showed essentially the same degree of intraindividual inconsistency: In almost 50 per cent of cases the tumor was graded differently at different times by the same pathologist. The inconsistencies might invalidate the usefulness of bladder tumor grading in clinical decision-making.

Humans↗
PubMed1983

Morphometric grading of bladder tumors in comparison with histologic grading by pathologists.

The authors describe the results of the grading of bladder tumors using morphometry and then compare these with results of histologic grading of the same tumors by different pathologists. Nuclear sizes of cells obtained from superficial and deep cell layers of each carcinoma and from giant cells were measured in 27 cases in which histologic tumor grading by different pathologists was unequivocal. In cells from all three areas, nuclear area increased with higher tumor grade. However, tumors of grades I and II showed significant differences in the size only of the large cells. It is concluded that morphometry is a valuable tool in the objective grading of bladder tumors, with the exception of carcinoma in situ lining the bladder lumen.

Carcinoma↗
PubMed1983

The association between precancerous-columnar and squamous lesions of the cervix: a morphometric study.

A histological study of 208 cases found a strong association between the occurrence of precancerous cervical columnar and squamous lesions. This was not, however, directly related to cellular abnormalities. The criteria for adenocarcinoma in situ (AIS) and its precursor lesions were defined morphometrically. With increasing abnormalities of the columnar cells, changes of the glandular and epithelial architecture were more frequently observed. Of the 20 cases morphometrically classified as AIS, only four were initially diagnosed as such, of which three were cases of 'pure' AIS lacking squamous cell abnormalities. Quantifying abnormalities of columnar epithelium is an important aid in the identification and classification of AIS.

Adenocarcinoma↗
PubMed1982

Morphometry and cytodiagnosis of breast lesions.

Cells aspirated from breast lesions and smeared on slides were subjected to computer assisted morphometric analysis. Three groups of cases were studied. The first were those collected from patients with known benign and malignant lesions. The second group were 143 unselected consecutive aspirates from breast lesions and the third, a group with a needle aspirate cytodiagnosis "suspicious of malignancy". The analysis showed the malignant cells to have larger nuclei with more anisokaryosis and more variation in the nuclear cytoplasmic ratios when compared with benign cells. When this form of semiautomatic analysis was applied to the "suspicious" group the accuracy of cytodiagnosis was improved.

Biopsy, Needle↗
PubMed1982

Monosodium glutamate accelerates migration of hypothalamic perikarya at puberty.

The administration of monosodium glutamate (MSG) to newborn rats accelerates by two weeks the migration of fluorescent dopaminergic perikarya from the median eminence towards the arcuate nucleus at the time of puberty, which normally occurs from day 22 till 55 after birth. It is hypothesized that MSG affects the trigger mechanism for this migration. The disappearance of fluorescent dopaminergic cells that occurs after MSG administration is considered as a secondary effect.

Animals↗