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Biomedical subjects

M E Boon

Publications and source records attributed to M E Boon.

At least 109 records · Page 6Linked to original sources
PubMed1988

Penile studies and heterosexual partners. Peniscopy, cytology, histology, and immunocytochemistry.

The male partners of 20 women with human papilloma virus (HPV) infection (subtypes 6, 11, 16, and 18) were studied. These men were unsuspecting and allowed investigation "for scientific reasons" in the context of the abnormal findings in their female partners. Peniscopy (study of the penis with a colposcope) was abnormal in 17 cases. In the cytologic-brush preparations of the urethra, hyperkeratosis (visualized with the modified Papanicolaou technique) occurred in all cases, but koilocytosis in only one. Both urothelial cells and squamous cells in the urethral samples were positive for HPV, as shown in the immunocytochemistry. In the histologic sections taken from flat lesions there was hyperkeratosis, dyskeratosis, and abnormal nuclear maturation. The authors concluded that, using simple techniques, subtle disturbances in maturation of nuclei and cytoplasm can be established in penile epithelium of these sexual partners. Similar changes are established in other HPV lesions. It seems likely that a significant proportion of the male population is infected in countries were HPV an important factor in cervical carcinogenesis.

Condylomata Acuminata↗
PubMed1988

Can cytomorphometry replace histomorphometry for grading of bladder tumours?

Cytomorphometry, using various cytopreparatory techniques on bladder washings and histomorphometry on the resected bladder tumours, was used in an attempt to answer the question: Can cytomorphometry replace histomorphometry for grading of bladder tumours? For the analysis of quantitative data, a probit model was used. Three out of the four cytomorphometric methods provided data supportive to the histomorphometry. Using one of the four cytomorphometric methods was sufficient to enhance grading accuracy and all were equally good. Two cases of high grade carcinoma in situ were properly identified by cytomorphometry (as judged on the follow-up data) but the concurrent resected papillary tumours were low grade. These findings indicate that cytomorphometry is a useful method in bladder tumour grading. In some cases it is preferable to histomorphometry.

Cytological Techniques↗
PubMed1988

Microwave irradiation of human brain tissue: production of microscopic slides within one day.

A three step method using microwave irradiation enabled microscopic slides of human brain tissue to be obtained within one working day: steps 1 and 2 hardened and solidified brain tissue; step 3 completed formalin fixation. The efficacy and precision of the method was compared with slides of conventionally processed brain tissue that had been fixed in formalin for six weeks. The microscopic quality of the sections was excellent with good presentation of brain tissue and equalled that of conventionally processed slides.

Adult↗
PubMed1988

Staining of the nucleolus with protein and RNA stains for automatic measurement of nucleolar size in paraffin sections.

In this paper two staining methods for automatic assessment of nucleolar profile area in paraffin sections of breast carcinomas using the IBAS 2000 image analyzer are reported. In the first method, the nucleolar proteins are stained with phloxine B, in the second the nucleolar RNA is stained with methyl green-pyronin. Both methods are fully reproducible. Statistically, the profile areas observed for the same patient by the two methods were found not to differ significantly. Because of their rigidity and their size in relation to section thickness, nucleoli are particularly attractive cell structures for quantitation, for instance in studies of variables that might be useful in establishing the prognosis for carcinoma patients.

Breast Neoplasms↗
PubMed1987

Efficacy of screening for cervical squamous and adenocarcinoma. The Dutch experience.

The nationwide screening program for cervical carcinoma in The Netherlands was unique in that all women between the age of 35 and 55 years were individually invited to undergo a cervical spatula smear. The total rate of positive histologic findings in six regions of the country was 3.9 per thousand, and decreased in the second screening after 3 years with 48% of patients. Further analysis of the change in rates of the separate histologic subgroups in six regions revealed: Severe dysplasia showed no change in rates. The rates of carcinoma in situ and invasive squamous cell carcinoma were influenced clearly by the screening. The preinvasive stages of adenocarcinoma of the cervix were underdiagnosed in the first screening, and there was no decrease in the score of invasive adenocarcinoma. The rates in the six regions differed significantly and were dependent on the degree of urbanization. Cervical screening using the Ayre spatula is not effective for detecting cervical adenocarcinomas.

Adenocarcinoma↗
PubMed1987

The microwave Rio-Hortega technique: a 24 hour method.

Application of microwaves in histochemistry and cytochemistry generally speeds up the technique. Microwaves stimulate diffusion into the tissue and influence the proteins and membrane of the cell. Silver impregnation techniques for the brain, such as the fast Rio-Hortega or the slow Golgi-Cox technique, normally require a minimum time period of 7 days and 20 days respectively. Using microwaves, the Rio-Hortega technique can be completed within 24 h. In sections prepared from mature brains, good silver impregnation of cell bodies, of axons and their terminals, and of dendrites and their spines are obtained. An explanation is given as to why the method cannot be further reduced in time. To our knowledge, this is the first report of the application of microwave irradiation for colouring pieces of tissue.

Animals↗
PubMed1987

Microwave-cryostat technique for neuroanatomical studies.

A novel method to process nervous tissue for neuroanatomical studies is presented. In this cryostat method, the brain tissue is first treated with microwave irradiation after perfusion with physiological saline. Cutting quality of the microwave-treated brain tissue was excellent. The sections were stained with the Nissl staining, the Klüver-Barrera method, and the Bodian method, respectively. In addition, immunocytochemistry using the RAM/PO method was performed. For the latter a monoclonal antibody against neurofilament (NF90), recognizing the 200, 150, and 70 kD units was used. Excellent results were obtained. The advantage of the microwave-cryostat technique for processing of nervous tissue is not merely a substantial reduction of time to obtain microscopical slides of good quality. This method allows in addition the estimation of the effect of fixation by formaldehyde in immunocytochemistry.

Animals↗
PubMed1987

Major improvement in microscopic-image quality of cryostat sections. Combining freezing and microwave-stimulated fixation.

In this article the authors describe a procedure to obtain cryostat sections of superior quality using microwave stimulation of a fixation reagent with suitable microwavability properties. In fact, the light microscopic images are of a quality that is comparable to that of the best conventional paraffin technique. The new procedure requires less time than the existing cryostat technique. The only additional piece of equipment needed in this procedure is a relatively inexpensive commercially available microwave oven.

Fixatives↗
PubMed1987

Floating on a water bath and mounting glycol methacrylate and hydroxypropyl methacrylate sections influence final dimensions.

This paper reports the dimensional changes occurring in the different steps of the histoprocessing of tissues for light microscopy. Two water-miscible methacrylates used for embedding, namely 2-hydroxyethyl methacrylate and 2-hydroxypropyl methacrylate, were investigated. It was found that during stretching on the water bath and in the mounting step considerable size changes occur, which are of the same magnitude as during the dehydration step of histoprocessing. The final dimensions of the sections and of microscopic images are dependent on the response to surface tension at the water surface and mounting of the glycol and hydroxypropyl methacrylate sections, respectively. Between the two resins under study, significant differences in the size of the resin sections, with and without embedded liver tissue, were found. It is shown that the temperature at which the sections are mounted is of great importance. These observations indicate the importance of standardizing histotechniques if morphometry is to be applied.

Acrylates↗
PubMed1987

Microwave-stimulated staining of plastic embedded bone marrow sections with the Romanowsky-Giemsa stain: improved staining patterns.

Staining plastic sections with the Romanowsky-Giemsa method is both time-consuming and difficult. This paper reports how the staining time can be reduced to 25 min using microwave irradiation of the staining solution. It is shown that staining results depend on the fixative used, staining temperature, dye concentration and pH of the staining solution as well as on several parameters of the microwave irradiation technique. The staining patterns are improved when compared with those obtained by conventional staining of plastic sections. The colors are more brilliant and greater contrasts are observed. Basophilia, polychromasia, and orthochromasia accompanying red cell maturation are more pronounced. For white cell maturation the initial appearance of specific granules (neutrophil, basophil, and eosinophil) is more evident. Thus, cell classification is easily accomplished using the described technique. It is suggested that microwave-stimulated staining be considered for routine use.

Animals↗
PubMed1987

Relation of quantitative features of visually normal intermediate cells in cervical intraepithelial neoplasia I and II smears to progression or nonprogression of the lesion.

The relationship between the quantitative features extracted from digitized images of visually normal intermediate cells in cervical intraepithelial neoplasia (CIN) I and II smears and the subsequent follow-up diagnosis was studied. Using the ISPAHAN interactive system of pattern recognition, the average rate of correct classification of progressive or nonprogressive lesions was 80% at the specimen level. It is concluded that additional diagnostic information regarding the probability of progression of CIN I and II lesions may be obtained by studying the microphotometric features of visually normal intermediate cells in the cervical smears. Significant differences were found between intermediate cells close to obviously dysplastic cells and those at a distance. The implication of this finding and the problems involved in the selection of the cells are briefly discussed.

Female↗
PubMed1986

Microwave-stimulated diffusion for fast processing of tissue: reduced dehydrating, clearing, and impregnating times.

In this paper we describe a method of preparing tissue blocks for paraffin sections within 30 min. The method is based on microwave-stimulated diffusion reducing the dehydrating, clearing, and impregnating times by a factor of 48. The developed technique was inspired by the experimentally observed sizable temperature-dependence of viscosity and other transport properties of liquids. It is clear that, by considering the theoretical aspects of diffusion and by analysis of the influence of the used chemicals in different tissue depths, histotechnical results can be optimized. The histotechnical microwave results are light-microscopically excellent and indistinguishable from those of the well-performed 'classical' method. The nuclear size of several cell types hardly differs in both methods. The new method is valuable in particular for individual cases in which a fast diagnosis is asked for, and in which a frozen-section diagnosis is thought to be too unreliable. In addition, this method can be used in small research laboratories processing small quantities of histological material. The only equipment needed to prepare tissue blocks of optimal quality is the microwave oven.

Cell Nucleus↗
PubMed1986

Connective tissue stroma in bladder papillary transitional cell carcinoma, carcinoma in situ and benign cystitis.

The stromal characteristics in papillary and non-papillary tumours of the urinary bladder were investigated in an attempt to improve the accuracy of histopathological diagnosis. It appeared to be possible to differentiate true papillary tumours from pseudopapillary structures lined by carcinoma in situ. Stromal differences were not found in cases of carcinoma in situ accompanied by denuding cystitis and cystitis due to other aetiological factors. It is concluded that histopathological examination of the stroma of bladder tumours improves diagnostic accuracy.

Alcian Blue↗