[Chronic rhinitis, or the consequences of gluttony].
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Adult↗
Biomedical subjects
M Drouet
Publications and source records attributed to M Drouet.
[Immunobiologic diagnosis of food allergy].
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators: Plasma histamine, tryptase, and urinary methylhistamine, to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.
Animals↗
[Histamine liberation in vitro or histamine release (HR). Study of the threshold of positivity in allergy to hymenopteran venoms].
The technique of histamine release in vitro (HR) is only usable in diagnosis of hymenoptera venom allergy. The allergen extracts that are supplied for clinical (skin tests) or biological diagnosis contain significant amounts of histamine, falsify the results, especially HR, and reduce significantly the correlation between HR and other biological or cutaneous tests. Dialysis of the allergen extracts significantly improves the sensitivity and specificity of the technique and lowers the threshold of positivity of HR from 30% to 12%. Correlation of HR with other diagnostic parameters:clinical history, specific IgE and skin tests is very significantly improved.
Animals↗
[Anaphylactic or anaphylactoid reactions to paracetamol. Report of 3 cases].
Anaphylaxis to paracetamol exists and perhaps nowadays diagnosis is not confined to clinical evidence of: The repeatability of clinical symptoms. Skin tests. Provocation tests. But also by immuno-biological evidence. Application of the test of Activation of basophils by Flow Cytometry and measurement of leukotriene C4 have been nowadays largely validated and constitute irrefutable proof of the responsibility of the drug as the origin of the clinical symptoms.
Acetaminophen↗
[Allergy to mosquitoes and use of new immunobiological parameters].
With regard to an observation of anaphylaxis after a mosquito bite, we have shown by new immunobiological parameters and clinical investigations: Skin tests, specific IgE, Tests of Activation of basophils by flow cytometry (on basophils and total blood) and measurement of Leucotriene LTC4, the objective reality of the mechanism of IgE-dependent hypersensitivity (HS). This observation is correlated with rare work in support of true IgE-mediated allergy to mosquito 9 authors having used skin tests, PK in 1984 and immunoblot.
Adult↗
[Acute or chronic food allergy: adapted therapeutic and diagnostic procedures].
In food allergy the allergen may present two very different clinical pictures: acute allergy (urticaria, bronchospasm, anaphylaxis) and chronic food allergy (eczema, rhinitis, digestive problems). These two syndromes are rarely separated in the literature on the subject although positive diagnosis is made on very different elements in the two cases. Furthermore, therapy, especially the diet, must be adapted in the two different cases of allergy.
Acute Disease↗
[Acute or chronic food allergy: adapted therapeutic and diagnostic procedure].
In food allergy the allergen may present two very different clinical pictures: acute allergy (urticaria, bronchospasm, anaphylaxis) and chronic food allergy (eczema, rhinitis, digestive problems). These two syndromes are rarely separated in the literature on the subject although positive diagnosis is made on very different elements in the two cases. Furthermore, therapy, especially the diet, must be adapted in the two different cases of allergy.
Acute Disease↗
[Immuno-biological diagnosis of food allergy].
Food allergy is becoming more frequent, with 6% of asthmatics reporting an isolated food allergy, and 5 to 6% of atopic dermatitis patients also have either a single or multiple true food allergy. There is value in immuno-biological diagnosis by: Measurement of total serum IgE. Measurement of mono-allergen-specific IgE, following a measurement by a multi-allergen of the Trophatope type. A study of elimination of foods for 2 or 3 months followed by their re-introduction. Oral provocation tests in a hospital environment under clinical control and subsequent measurement of the mediators:-Plasma histamine, tryptase, and urinary methylhistamine to give proof of responsibility of the food allergen. Nowadays, it is perfectly possible to include in diagnosis the new technologies of the test of activation of basophils/or lymphocytes by means of flow cytometry.
Adolescent↗
[Cross-reactivity between cow's milk and goat's milk].
Tolerance of LC may be explained by a great intra and interspecific genetic diversity that leads to a great variety of proteins. In contrast, industrial treatment (UHT sterilisation) introduces configural modifications that may provoke a very great structural homology between the proteins of the two milks. Casein is in high concentration in both milks and shows some homology of the AA sequences between both and it makes a common allergen for a cross- reaction. It has been observed that a good IgE inhibition in vitro does not automatically form a clinical cross-allergenicity. Therefore there may exist an immunological crossed reaction between LC and LV without production of a real crossed allergenicity.
Animals↗
[When the fluoro-immuno-enzymatic (FEIA) measurements turn out to be more sensitive than radioimmunologic (RIA) measurements. Application to the measurement of serum tryptase].
The measurement of Tryptase by the Fluoro-Immuno-Enzymatic (FEIA) method is nowadays possible on the Pharmacia CAP system (automatic UniCAP). This measurement is more comprehensive as it measures the release of serum tryptase from both the tissue mastocytes (MCTC) as well as the mucosal mastocytes (MCM). Technically the measurements are comparable with those made by the method of radio-immunology (RIA), are absolutely reproducible and surprisingly at 100%. It has also been possible to evaluate the two techniques of FEIA and RIA on negative and positive pools. This new FEIA technique for serum tryptase is applicable: to anaphylactic and/or anaphylactoid accidents at the time of induction of anesthesia, in general conditions such as haemorrhagic recto colitis (RCH), Crohn's disease, and mastocytosis. Finally these measurements can be used during nasal and bronchial provocation tests, as the measurements may be made on nasal and bronchial lavage liquids. The sensitivity and the very good reproducibility of this new technique of FEIA for tryptase is of very great interest and avoids use of radio-active isotopes.
Anaphylaxis↗
[Comparison of the measurements of total IGE using the CAP-RIA technique and the UNICAP-FEIA technique].
The contribution of a new method of measurement of total IgE by the Fluoro-Enzymo-Immuno-assay (FEIA) with the assistance of Pharmacia UNICAP automation is very reliable since, when compared with the measurement by the CAP SYSTEM RIA on 144 patients who were clinically allergic (84 with a raised and 60 with a normal IgE titre) the result of a comparative study between the two techniques is statistically highly significant. The two methods were also compared by age groups and these results were also statistically highly significant.
Adult↗
[Treatment with cyclosporin A, with low doses, in high-risk penetrating keratoplasties. A bi-centric study of 90 cases].
PURPOSE: Evaluation of cyclosporin-A in prevention of immune reaction in high-risk penetrating keratoplasties. MATERIAL AND METHODS: Cyclosporin A was given to 45 corneal allograft recipients, 5 mg/kg/j (cyclosporinemy between 100 and 150 ng/l), for three months following surgery. 45 controls have undergone penetrating keratoplasty during the same period. Mean follow-up was respectively 431 days and 402 days. Survival was analysed according to Kaplan-Meier's method, and then using Cox model. RESULTS: The significant predictive factors were the number of neovascularized quadrants, and the graft diameter. No significant effect of cyclosporin is evidenced. Side effects are marginal. CONCLUSION: Three hypothesis may explain the absence of prognosis improvement in the Cyclosporin A treated group: insufficient dose or duration of treatment, individual risk factors that prevents correct pairing, or corneal-specific immunological mechanisms.
Cyclosporine↗
[Correlative study of the anti-gluten IgA level in celiac disease. Apropos of some observations].
This work concerns the level of anti-gluten IgA in Coeliac disease, following subjects who have already been diagnosed by clinical signs, biopsy and measurement of anti-gluten IgA. Even if this study had been limited only to a single patient, it would have shown well the clinical sensitivity to these levels, so allowing the following of the development, correlated with improvement of the clinical symptoms with lowering of the level of anti-gluten IgA.
Aged↗
C-1-inhibitor binding monoclonal immunoglobins in three patients with acquired angioneurotic edema.
The syndrome of acquired angioneurotic edema (AAE) is characterized by the adult onset of angioedema, the lack of evidence for inheritance of the disorder, and the frequent association of the C1-inhibitor (C1-INH) deficiency with lymphoproliferative or other malignant diseases. Recently, a new type of AAE (type II AAE) has been described. The two major biologic differences of this new syndrome compared with all other previously reported AAE cases (type I AAE) are the presence in patients' sera of both anti-C1-INH autoantibodies, often monoclonal, and a circulating low molecular weight (95 kd) C1-INH protein. From the clinical point of view, the absence of underlying lymphoproliferative disease is the hallmark of type II AAE compared with type I AAE. However, the distinction between type I and type II AAE may not be so clear-cut. We report three patients with monoclonal gammopathies and AAE for whom the initial diagnosis was type I AAE. The demonstration by ELISA of the C1-INH binding ability of their monoclonal immunoglobulins in addition to the presence of 95 kd C1-INH protein enables us to change the diagnosis to type II AAE. From the therapeutic point of view, it is crucial to detect the anti-C1-INH antibody and to analyze the C1-INH size to distinguish type I and type II AAE, especially if patients have a monoclonal gammopathy, to give the appropriate treatment (attenuated androgens vs immunosuppressive regimen, respectively) to prevent a fatal outcome.
Aged↗
[Assay of IgE specific to dog epithelia by the Pharmacia CAP RAST technique: comparison of the two CAP RAST e2 and e5].
We present a comparative study of measurement of dog-epithelium-specific IgE by two Pharmacia CAP Rast techniques e2 and e5. For both measurements the technique is identical, but the allergen extracts are different: e2 = dachshund extract whilst e5 = a mixture of squames of german shepherd and poodle. We show that the e5-specific IgE seems to be more sensitive than the e2-specific IgE.
Allergens↗
[Anaphylactic reaction to Aspergillus].
Nowadays the pollens are the principal allergens implicated in anaphylactic reactions. Now one of our observations is of a case history of recurring acute urticaria, with malaise, hypotension, Quincke's oedema and dyspnea, linked with Aspergilus. The etiology is based on clinical observation, skin tests and specific IgE with a significant atopy. This observation shows that the anaphylactic reaction can also be associated with a mould such as Aspergillus.
Administration, Sublingual↗
The pork/cat syndrome or crossed reactivity between cat epithelia and pork meat.
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Adult↗
[Oxidative stress and apoptosis].
Programmed cell death or apoptosis is a process characterized by several morphological, biochemical and molecular events in response to physiological or pathological stimuli, such as gamma radiation. Free radicals being involved in many physiological and pathological processes, the aim of this study is to investigate the literature about the involvement of oxidative pathway during apoptotic process. As reported by several authors, the literature is abundant in this field and show the complexity of the network in which numerous molecules can regulate cell death and proliferation. However, reactive oxygen intermediate species (ROls) seem to play an important role in the induction of apoptosis as underlined by several reports. Regulation of cellular redox status may appear to be a key component which determines cell proliferation or apoptosis.
Animals↗