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Biomedical subjects

M Donati

Publications and source records attributed to M Donati.

At least 91 records · Page 5Linked to original sources
PubMed1980

Non-gonococcal urethritis: epidemiological and etiological study in Italy.

The Authors report the results of a study on the etiology of non-gonococcal urethritis (NGU) carried out on 180 male patients with NGU and on 50 healthy volunteers matched for age and sex. Chlamydia trachomatis infection was proven by culture in 54 (30%) of the NGU-patients and in none of the healthy controls. Ureaplasma urealyticum was recovered from men both with and without urethritis. Bacteria other than C. trachomatis were not found in NGU-patients in a significant percentage. All C. trachomatis-positive NGU-patients presented antibodies against C. trachomatis, by indirect fluorescence, while only 54% of C. trachomatis-negative NGU-patients and 22% of healthy volunteers, were positive. A further serological screening of 78 women with no history of urethritis or cervicitis and 44 children less than 10 years of age was carried out in order to investigate the prevalence of serum antibodies against C. trachomatis in groups of healthy population in Italy.

Adolescent↗
PubMed1979

[The replication cycle of Chlamydia trachomatis and Chlamydia psittaci: ultrastructural analysis].

An ultrastructural comparative analysis is reported on the replication cycle of six strains of Chlamydia trachomatis, recently isolated, and Chlamydia psittaci strain 6BC, grown in cell cultures. Important morphological distinctions of the bacterial walls are documented. In Chlamydia trachomatis the cell wall seems more rigid. This causes, during the morphogenesis of the elementary body, its separation from the cell membrane with formation of an electron transparent space, which is not demonstrable in Chlamydia psittaci. As a result of this process, the appearance of "ghosts" and "mini reticular bodies" is documented. Budding and multiple fission are suggested as possible mechanisms of replication of Chlamydia trachomatis, in coexistence or/as alternative to binary fission.

Cell Wall↗
PubMed1979

[Antibodies against Chlamydia trachomatis in patients with non-gonococcal urethritis and in the healthy Italian population].

The Authors report the results of a study on the prevalence of serum antibodies against Chlamydia trachomatis among patients with non-gonococcal urethritis caused by Chlamydia trachomatis and diagnosed on the basis of the isolation of the microorganism in cell cultures, among patients with non-gonococcal urethritis of unknown etiology and in groups of healthy population, in Italy. The search for antibodies was performed both with complement-fixation tests in micro-titer system, using an antigen prepared from chicken embryo yolk sacs infected with the 6BC strain of Chlamydia psittaci, and with indirect immunofluorescence tests. The indirect immunofluorescence tests were carried out using as antigen the whole chlamydial inclusion developed in IUDR pre-treated McCoy cell cultures, grown on coverslips, and infected with the BU/434 strain of the LGV2 immunotype of Chlamydia trachomatis. The immunofluorescence tests proved to be much more sensitive than the complement fixation tests in detecting the presence of anti-chlamydial antibody both in patients with non-gonococcal urethritis and in the healthy population groups. The percentage of subjects possessing anti-chlamydial antibodies among the healthy population suggests a diffuse circulation of Chlamydia trachomatis infection. The possibility of employing the serological data in the diagnosis of the etiology of non-gonococcal urethritis is briefly discussed.

Adolescent↗
PubMedDate not supplied

Detection of Chlamydia trachomatis by a ligase chain reaction amplification method.

BACKGROUND AND OBJECTIVES: The ligase chain reaction is an in vitro DNA amplification technique that exponentially amplifies selected DNA sequences. GOAL: To evaluate a ligase chain reaction assay for the detection of Chlamydia trachomatis cryptic plasmid DNA (LCx Chlamydia) in patients routinely attending a sexually transmitted disease center in Italy. STUDY DESIGN: Urethral or cervical swabs were obtained from 501 consecutive patients (334 men and 167 women). The samples were assayed in parallel with LCx Chlamydia and conventional tissue culture; discordant results were further assayed by direct immunofluorescence and a ligase chain reaction with alternate primers. RESULTS: After resolution of discordant results, the LCx method showed a sensitivity, specificity, positive predictive value, and negative predictive value of 100%, 99.3%, 96.7%, and 100% in men; 100%, 100%, 100%, and 100% in women; and 100%, 99.5%, 97.1%, and 100% overall, respectively. By comparison, the sensitivity of tissue culture was 81.4% in men, 50% in women, and 77.6% overall. CONCLUSIONS: The automated LCx method is sensitive, fast, and accurate and represents a useful diagnostic tool for C. trachomatis infection, even in low and medium prevalence populations.

Chlamydia Infections↗
PubMedDate not supplied

[Organizational and surgical-technical aspects of the service of abdominal hernioplasty].

The authors guided by the experience matured from 1/01/94 to 30/06/97 (435 abdominal hernioplasties performed, mainly inguinal and femoral) illustrate the organizational formalities and the technical aspects of a Hernia Surgery Service. In particular, they highlight the advantages of the routine use of local anaesthesia and of tension-free techniques, carried out on a day surgery rule (immediate rehabilitation, greater facilitation to elective surgery, access to the elderly at high anaesthetic risk). Eventually, they emphasize the remarkable reduction of sanitary costs and the outstanding social, practical and didactic value of such Hernia Centers.

Anesthesia, Local↗
PubMedDate not supplied

[Hernia repair with local anesthesia].

The authors report the results of their own personal experience with inguinal and femoral hernioplasties (424 cases) performed under loco-regional anaesthesia. This anaesthetic approach together with the use of prosthetic techniques (tension-free and suture-less) represent the "Gold Standard" in the surgical treatment of hernia. The absence of mortality, the remarkable reduction in terms of postoperative complications, days of hospitalization (one day surgery) represent outstanding advantages. The cooperation of the patient and the stress test at the end of the operation are further advantages of the proposed technique.

Adult↗
PubMedDate not supplied

[Inguinal hernioplasty with calibrated prosthesis (personal modification of the Trabucco technique].

From January 1994 to December 1997, the authors operated on 636 patients with primary inguinal hernia, using an original modification of Trabucco's tension-free and sutureless technique. The hernial defect is sized according to Gilbert's classification modified by Rutkow and Robbins and repaired with one or more plugs (up to four in the authors' experience) in relation to its extent. The posterior wall is reconstructed over the plugs encompassing the transversalis fascia and a wing of the plug superiorly and the iliopubic tract inferiorly. The double-layer polypropylene mesh is fashioned intraoperatively and the key-hole tailored to the variable distance between the spermatic cord and the pubic tubercle. All patients ambulated immediately after the operation, had a light meal two hours later and were discharged within one day of surgery. During a 3 months to 4 year follow-up only one recurrence has been recorded (0.16%). The proposed technique allows to perform a repair to measure ("calibrated") with minimal pain, immediate rehabilitation and early return to unrestricted activity.

Aged↗
PubMedDate not supplied

[Current approach in the treatment of desmoid tumors of the abdominal wall].

Desmoids of the abdominal wall are rare tumours with an incidence of 2-4 cases per million. From our experience which is reported here in detail (5 cases observed over the past 20 years), we draw the conclusion that the only completely reliable diagnosis is still histological, in spite of the use of ultrasound, CT scans and the new imaging possibilities afforded by NMR. After comprehensive excision of at least 2 cm of macroscopically healthy tissue from the tumour margins, reconstruction using prosthetic materials, such as Mersilene, PTFE or Prolene was performed. Neither radiotherapy nor chemotherapy were used, since there were doubts as to their usefulness. The functional and cosmetic results were satisfactory in all cases. Over follow-up periods ranging from 3 to 13 years, none of the patients has presented recurrences or incisional hernias.

Abdominal Muscles↗
PubMedDate not supplied

[Tension-free lumbar hernioplasty].

The authors report a case of a 51 year-old woman with spontaneous Grynfeltt's hernia. Computerized tomography accurately displayed the anatomy of the lumbar region, thus aiding diagnosis and treatment. A double layer polypropylene mesh was used for the repair, placed in the pre peritoneum and sutured to the margins of the hernial defect avoiding muscles and fascias. The use of a double layer prosthesis and reduction of tension on the suture line allow a sound repair, with minimal postoperative pain and immediate resumption of daily activities.

Female↗
PubMedDate not supplied

[Tension-free prosthetic repair in the surgical treatment of epigastric hernia].

From November 1994 to June 2001, the authors have performed 16 operations for epigastric hernia. Local anaesthesia was used in 8 patients with small hernias, in the remaining 8 general anaesthesia was preferred. In defects smaller than 4 cm, the repair was achieved by insertion of a polypropylene dart plug sutured to the margins of the hernial defect. In larger defects a double layer mesh placed in the pre-peritoneum was used. All patients were discharged within 24 hours of surgery. Postoperative pain was mild and required hospital analgesia in 25% of cases and domiciliary analgesia in 1 patient only. During a follow-up ranging 3 to 79 months (mean 31.2), no recurrence has been recorded. The proposed technique is simple, safe and absolutely effective, allows immediate rehabilitation with a low risk of complications.

Adult↗
PubMedDate not supplied

[Surgical tactics in abdominal wall neoplasia].

Abdominal wall neoplasms represent more or less 1% of human neoplasms in the adult. The authors reports their own experience based on 9 cases during over 20 years. The prognosis of these tumors is almost influenced from a lot of factors such as: histology, localization, staging, grading, sex, surgical margins, number of local recurrences. Abdominal wall neoplasm are less aggressive for compartmentalization of muscle layer and with a better prognosis because of their localization, and surgical opportunities of extensive resection (not less of 2 cm from tumor's macroscopic limits) allowed by modern prosthetic reconstruction techniques. Polipropilene seems to be the ideal material for such kind of reconstruction even if also mersilene, PTFEe and others were employed. PTFEe and Dual-meshes could be useful in those malignant tumors in which peritoneum resection is necessary.

Abdominal Neoplasms↗
PubMedDate not supplied

[Bilateral breast cancer].

Bilateral breast cancer has a cumulative incidence of 3-13% in patients with primary breast cancer, and the majority of these lesions are metachronous. The synchronous and metachronous bilateral breast cancer have at greater risk for distant metastasis than unilateral breast cancer. There was no difference in overall survival when comparing metachronous and synchronous bilateral patients to those with unilateral disease.

Adult↗
PubMedDate not supplied

[Undifferentiated carcinoma of the thyroid].

Anaplastic thyroid carcinoma is a rare and highly lethal cancer. Between 1969 and 2002, 9 cases with anaplastic carcinoma were reviewed. Surgical treatment with radiotherapy and chemotherapy were performed in all cases. All patients died of their disease within 5 years. The mean survival was 10 months. There is still controversy as to what constitutes adequate treatment for anaplastic thyroid carcinoma, and combined multimodal therapy seems to be the most common management strategy for this aggressive disease.

Adult↗
PubMedDate not supplied

Analogic evaluation of pain during inguinal hernioplasty under local anaesthesia.

OBJECTIVE: The authors evaluate intraoperative pain in patients undergoing tension-free inguinal hernioplasty under local anaesthesia. MATERIAL AND METHODS: One hundred and fourteen primary inguinal hernia repairs were carried out at the Department of General Surgery I of Catania University Polyclinic from January to September 2002. 2% Mepivacaine cloridrate was the local anesthetic of choice. Intraoperative pain was measured by a visual analog scale. RESULTS: A mean analogic score of 1.9 (range 0-2.9) was obtained. Intraoperative complications were recorded only in 2 patients (1.7%). There was no operative mortality. All patients were up and about straightaway after surgery, had a light meal two hours later and were discharged within one day of operation. CONCLUSIONS: Inguinal hernia repair under local anaesthesia is well tolerated and is associated with a low risk of complications.

Adult↗
PubMedDate not supplied

Plug-technique for small incisional hernia.

OBJECTIVE: The authors report their experience with a new prosthetic technique for the repair of incisional hernia defects smaller than 3 cm. METHODS: From January 1995 to September 2002, 16 operations for small incisional hernias have been performed. Local anaesthesia was used in 12 out of 16 patients (75%). The repair was achieved by insertion of a polypropylene plug sutured to the margins of the hernial defect. RESULTS: All patients were discharged within 24 hours of surgery. Postoperative pain was mild and required hospital analgesia in 25% of cases. Back at home analgesia was needed in only 1 patient. During a follow-up ranging 3 to 91 months (mean 46), no recurrence has been recorded. CONCLUSION: The proposed technique allows a sound repair of small incisional hernias with minimal pain, quick rehabilitation and early return to unrestricted work.

Abdomen↗
PubMedDate not supplied

[Endometriosis of the abdominal wall (authors' experience)].

Endometriosis is a very common gynecological disease, although the abdominal wall localization is a rare clinical problem in the everyday practice and this explains the incomplete reports in literature and the difficulty of a standard treatment. The authors report four cases of abdominal wall endometriosis, underlining how the surgical therapy represents the golden standard of decisive treatment, thanks also to prosthetic reconstruction techniques who allow more radical demolitions. On the contrary, the medical treatment should be reserved to selected cases such as especially the less symptomatic umbilical primitive diseases.

Abdominal Wall↗