Glutathione depleting agents and lipid peroxidation.
The mechanisms by which glutathione (GSH) depleting agents produce cellular injury, particularly liver cell injury have been reviewed. Among the model molecules most thoroughly investigated are bromobenzene and acetaminophen. The metabolism of these compounds leads to the formation of electrophilic reactants that easily conjugate with GSH. After substantial depletion of GSH, covalent binding of reactive metabolites to cellular macromolecules occurs. When the hepatic GSH depletion reaches a threshold level, lipid peroxidation develops and severe cellular damage is produced. According to experimental evidence, the cell death seems to be more strictly related to lipid peroxidation rather than to covalent binding. Loss of protein sulfhydryl groups may be an important factor in the disturbance of calcium homeostasis which, according to several authors, leads to irreversible cell injury. In the bromobenzene-induced liver injury loss of protein thiols as well as impairment of mitochondrial and microsomal Ca2+ sequestration activities are related to lipid peroxidation. However, some redox active compounds such as menadione and t-butylhydroperoxide produce direct oxidation of protein thiols.