Biomedical subjects

M Colledge

Publications and source records attributed to M Colledge.

8 recordsLinked to original sources

PubMed1993

Clustering of the acetylcholine receptor by the 43-kD protein: involvement of the zinc finger domain.

A postsynaptic membrane-associated protein of M(r) 43,000 (43-kD protein) is involved in clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Previous studies have shown that recombinant mouse 43-kD protein forms membrane-associated clusters when expressed in Xenopus oocytes. Coexpression with the AChR results in colocalization of the receptor with the 43-kD protein clusters (Froehner, S. C., C. W. Luetje, P. B. Scotland, and J. Patrick, 1990. Neuron. 5:403-410). To understand the mechanism of this clustering, we have studied the role of the carboxy-terminal region of the 43-kD protein. The amino acid sequence of this region predicts two tandem zinc finger structures followed by a serine phosphorylation site. Both Torpedo 43-kD protein and the carboxy-terminal region of the mouse 43-kD protein bind radioisotopic zinc. Mutation of two histidine residues in this predicted domain greatly attenuates zinc binding, lending support to the proposal that this region forms zinc fingers. When expressed in oocytes, the ability of this mutant 43-kD protein to form clusters is greatly reduced. Its ability to interact with AChR, however, is retained. In contrast, a mutation that eliminates the potential serine phosphorylation site has no effect on clustering of the 43-kD protein or on interaction with the AChR. These findings suggest that protein interactions via the zinc finger domain of the 43-kD protein may be important for AChR clustering at the synapse.

Amino Acid Sequence↗

PubMed1982

Economic cycles and health. Towards a sociological understanding of the impact of the recession on health and illness.

Does the impact of large scale and long term unemployment have a negative influence on the health of a community? This paper critically examines research evidence at a macro and micro level of analysis debating the major theoretical issues, and the difficulty in establishing causality between changing mortality patterns over time and fluctuations in the business cycle. It is argued that macro analysis cannot stand on its own as an explanation but needs to be combined with an understanding of the career progression of the unemployed and the stages of psychological transition that occur to bridge the gap between macro and micro environments, and give some understanding of the changes in health status that may be observed in a population following a period of unemployment or economic uncertainty. Both quantitative and qualitative research suggest that not only is the health of those actually at risk but also their immediate family and the community at large during a recession. From this proposition two significant factors emerge in the literature which could explain the association between unemployment rates and changing patterns of mortality. Firstly stress is highlighted in most of the research reviewed, and could be the linking factor for material reasons and also those of social well being. Secondly health status, like that of unemployment is not equally shared in the United Kingdom community, but rather shouldered on a class basis, the most consistent finding being an inverse relationship between economic status and mortality placing the burden squarely on the lower social economic groups who are most vulnerable to unemployment. Taken together all the evidence suggests the inferences made from the macro statistics despite the difficulties in demonstrating causality, suggesting a strong link between mortality rates and cycles of economic activity.

Adult↗

PubMed1974