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Biomedical subjects

M Cerný

Publications and source records attributed to M Cerný.

At least 55 records · Page 3Linked to original sources
PubMed1985

[The karyotype and character of chromosome bands in mountain sheep in Czechoslovakia].

Thirty-one moufflon (Ovis musimon) coming from eight localities in Bohemia and Moravia were examined. All animals had a diploid number of chromosomes without any numerical or structural aberrations from the standard set. On the basis of G-banding, all chromosomes were arranged into homologous pairs. C-banding was used to prove the localization of constitutive heterochromatin in the centromeric regions of the majority of chromosomes. Nucleolus organizers, demonstrated by means of A-staining, were located telometrically on the chromosomes in three big two-arm pairs and two acrocentric pairs. The number of active nucleolus organizers in individual cells ranged from 2 to 7. When these results were compared with the published data on the karyotype of sheep, the two forms were found to have much in common. It can be supposed that the karyotypes of moufflon and domestic sheep differ in the frequency of the occurrence of Ag-positive sections with nucleolus organizers.

Animals↗
PubMed1981

Inhibition of in vitro biosynthesis of N-acetylneuraminic acid by N-acyl- and N-alkyl-2-amino-2-deoxyhexoses.

The biosynthesis of N-acetylneuraminic acid is markedly inhibited by 2-deoxy-2-propionamido-D-glucose (GlcNProp) and to a much lesser extent by 2-deoxy-2-propionamido-D-mannose (ManNProp), but not by 2-deoxy-2-propionamido-D-galactose and N-methylated derivatives of 2-amino-2-deoxy-D-glucose. 2-Deoxy-2-trimethylamino-D-glucose is a weak inhibitor of 2-acetamido-2-deoxy-D-mannose metabolism. When incubated in a cell-free system from rat liver, GlcNProp gives the 6-phosphate, which is converted into N-propionylneuraminic acid. Evidence is presented which shows that it is the metabolites GlcNProp-6-P and ManNProp-6-P which are the competitive inhibitors, and not GlcNProp itself.

Animals↗
PubMed1981

Posttetanic inhibition in the guinea - pig ileum by endogenous opiate ligands; effects of cold storage of the preparations and of the age of experimental animals.

Naloxone-sensitive inhibition of neurogenic twitch contractions was induced by tetanic stimulation of the myenteric plexus-longitudinal muscle preparation of the guinea-pig ileum. In preparations stored in the cold for more than 2 days, the inhibition disappeared and in some preparations posttetanic potentiation was manifested, instead. Thus enkephalinergic neurones are more sensitive to the effect of cold storage than cholinergic neurones. The naloxone-sensitive posttetanic inhibition was developed in newborn animals but in animals older than 3 years a naloxone-resistent component appeared.

Acetylcholine↗