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M C Bene

Publications and source records attributed to M C Bene.

At least 55 records · Page 3Linked to original sources
PubMed1990

Pre-B lymphocytes with intracytoplasmic mu chains in the peripheral blood of rheumatoid arthritis patients.

A dysregulation of B-cell differentiation and activation has long been evidenced in rheumatoid arthritis (RA). Such analyses have, however, usually focused on the latest stages of B-cell development. Using a classical technique of immunofluorescence labeling on cytospins, we investigated the presence of peripheral pre-B lymphocytes in 92 RA patients and 23 controls. Cells with intracytoplasmic mu chains were evidenced in 58.7% of the RA patients studied, ranging between 0 and 30%, while small numbers of c-mu cells, never exceeding 6% of peripheral blood lymphocytes, were observed in 9 controls. Relationships between this feature and clinical or laboratory data were investigated, showing a negative correlation between the percentage of c-mu + lymphocytes and Ritchie's index (P = 0.05), the number of tender or swollen joints (P = 0.05), erythrocyte sedimentation rate (P = 0.005), seropositivity (P = 0.05), and disease duration (P = 0.01).

Adult↗
PubMed1990

A CD4-like molecule can be expressed in vivo in human parathyroid.

The expression of several epitopes of CD4, a molecule usually restricted to a subset of T-lymphocytes, was observed after immunofluorescent labeling of frozen-cut sections of human parathyroid. Seven samples obtained from three subjects presenting adenomas and from seven renal insufficiency patients with secondary or tertiary hyperplasia were found to express this molecule. Dot enzyme-linked immunosorbent assay, polyacrylamide gel electrophoresis, and Western blotting were used to characterize this peptide in cytosol and membrane fractions of these glands. These studies confirmed, in the parathyroids found positive in immunofluorescence, the presence of a protein with a mol wt similar to that of lymphocyte-derived CD4.

Adult↗
PubMed1990

[Immunohistology of polyps of the nose and paranasal sinuses. Physiopathologic value].

The pathophysiology of nasal polyposis is yet unclear, but chronic disturbances of the inflammatory pathways may be suspected. Immunological investigations were performed in 3 series of patients: 37 with nasal polyps, 22 with chronic sinusitis, and 15 controls with healthy nasal and sinusal mucosa. Patients with nasal polyps had up to ten times more eosinophil density than patients with sinusitis or healthy mucosa. The average of mast cells and IgE or IgA, M, G plasma cells were not different in the various group. Deposited immune complexes were always absent. These results gave us the opportunity to discuss recent pathogenic theories.

Adolescent↗
PubMed1989

Correlations between acute lymphoid leukemia (ALL) immunophenotype and clinical and laboratory data at presentation. A study of 350 patients.

The phenotypes of malignant cells from 350 untreated patients with acute lymphoblastic leukemia (ALL) were determined at diagnosis with the use of a panel of monoclonal antibodies to leukocyte antigens. According to the phenotypes seen, the cases were divided into five groups, pre-B ALL, B-ALL, T-ALL, MO-ALL, and undifferentiated ALL. Each group was subdivided, resulting in 11 defined immunologic subtypes. Correlations between clinical and laboratory features were investigated at presentation. ALL of early-B phenotype associated with elevated cell counts occurred more often in female and infant patients than in male patients. Involvement of the central nervous system was frequent in B-ALL, which occurred mostly in male patients. A male prevalence was also seen in ALL of T-lineage in which significant differences regarding clinical characteristics and leukocyte counts appeared among the four subtypes. The clinical relevance of phenotypic subcategorization is supported by our observations.

Antibodies, Monoclonal↗
PubMed1989

Investigation of the CD10 (cALLA) negative acute lymphoblastic leukaemia: further description of a group with a poor prognosis. French Groupe d'Etude Immunologique des Leucémies.

The absence of CD10 (cALLA) in non-T non-B acute lymphoblastic leukaemia (ALL) is usually considered to be of adverse prognostic significance. From a large multicentre series of phenotyped ALL, we have identified a group of 23 non-T non-B ALL where blast cells were CD10 negative and CD19 positive. Class II antigens were present in 80% and C19 in 70%. Eight patients had successful karyotype analysis at diagnosis, and an additional patient at first relapse. Seven of these karyotypes showed a (4;11) (q21;q23) translocation. Most of the patients (70%) were young females, and they often presented with organomegaly. Six patients were less than 1 year old. The white cell count was over 100 x 10(9)/l in 48% of the cases. The FAB type was L2 in 56% of the patients. The most striking features were the poor response to therapy and survival. Six patients never attained complete remission and nine patients relapsed, most of them during the first year after diagnosis. Allogeneic bone marrow transplantation was performed in three children, of whom two are still alive 2 years after diagnosis. This study emphasizes the prognostic value of immuno-phenotypic and karyotypic investigations of ALL.

Adolescent↗
PubMed1989

Decreased lymphocyte function-associated antigen-1 molecule expression on peripheral blood lymphocytes from patients with Graves' disease.

Humoral and cellular abnormalities have been described in autoimmune thyroid diseases, but a deficient cooperation between humoral and cellular systems has been poorly investigated in such diseases. Lymphocyte function-associated antigen-1 (LFA-1), a glycoprotein expressed on T- and B-lymphocytes, plays an important role in cell-cell interaction mechanisms and is involved in the homing phenomenon. The expression of this molecule was investigated on peripheral blood lymphocytes from patients suffering from various thyroid disorders. Decreased numbers of LFA-1-positive cells were observed in 30 patients with Graves' disease, while normal values were present in 8 patients with Hashimoto's thyroiditis and 37 subjects with nonautoimmune thyroid disorders. Treatment-induced improvement of Graves' disease was associated with an increase in LFA-1-positive cell number.

Adult↗
PubMed1989

[Change in the lymphocyte subpopulations in bronchoalveolar lavage 72 hours after the allergen provocation test in the asthmatic].

Fourteen patients suffering from perennial allergic asthma linked to mono-sensitivity to the house dust mite were studied. An initial bronchoalveolar lavage with standard cytology as well as a study of the T lymphocyte subpopulations (CD3, CD4, CD8, Leu 7) and B cells (CD19) was carried out, representing the basal situation. Four weeks later an allergic bronchial provocation test to purified extracts of the house dust mite was performed taking care to monitor the occurrence of any delayed bronchial response (8 out of 14). A second bronchoalveolar lavage was performed 72 hours after the bronchial provocation test. A significant rise of eosinophils in the bronchoalveolar fluid was registered 72 hours after the provocation test; m +/- sem (%): 3.07 +/- 1.31 vs 7.78 +/- 1.22; p less than or equal to 0.005, Student t test for paired series. This large rise in eosinophils was observed independently of the type of response, whether immediate or biphasic. The CD4 lymphocyte subpopulations were significantly decreased after bronchial provocation tests m +/- sem (%) 12.21 +/- 2.79 vs 6.47 +/- 1.62; p less than or equal to 0.05 Student t test for paired series. There was no overall significant difference after the provocation tests in the CD8 lymphocyte subpopulation. However, a remarkable and significant rise was noted in the CD8 populations after bronchial provocation in 6 subjects presenting with an immediate isolated asthmatic reaction; m +/- sem (%) 10.17 +/- 4.01 vs 34 +/- 7.32, p less than 0.05, paired Wilcoxon test.(ABSTRACT TRUNCATED AT 250 WORDS)

Adolescent↗
PubMed1989

Isotype evolution in the follow-up study of patients with Campylobacter pylori associated gastritis.

Four sequential immuno-assays were performed from May to November 1988 to follow the levels of IgG, IgA and IgM to Campylobacter pylori in 16 infected patients with histologically proven gastritis, among which 12 received appropriate therapy. Histopathological examination of antral biopsies, bacteriological cultures and urease tests were performed on each occasion when serum was tested for antibodies to C. pylori. The detection and quantitative assessment of the various isotypes to this bacterium proved valuable to appreciate the response to therapy with, in case of success, a steady decrease of antibodies levels concomitant with clinical improvement.

Adult↗
PubMed1989

[Prevalence of non-thyroid autoantibodies in autoimmune dysthyroidies].

Organ- and non organ-specific autoantibodies can be detected in patients with AITD but large comparative studies have seldom been performed. This study evaluated the prevalence of anti-thyroid, -smooth muscle, -mitochondria, -parietal gastric cells, -salivary duct, -nuclear and -ds DNA autoantibodies assayed by indirect immunofluorescence in 224 patients with Graves' disease or Hashimoto's thyroiditis. Results evidenced a high prevalence of antinuclear antibodies, mostly of non homogenous fluorescence in Graves' (63.1%) and Hashimoto's patients (65.5%), as well as for antisalivary duct antibodies (55.2 and 75%). No positive anti-ds DNA were noticed. No correlation was found between antithyroid antibodies and the others. Different hypothesis could explain this observation which favours a general dysregulation of the autoimmune system.

Adolescent↗
PubMed1989

Cowden disease in a young girl: gynecologic and immunologic overview in a case and in the literature.

There is an increased risk of cancer (mainly breast cancer) in patients with Cowden disease. Little is known of the etiopathogenic mechanisms of this condition, but immunologic abnormalities may be evoked. A young patient with Cowden disease was submitted for gynecologic examination and immunologic investigations. Although no neoplastic disorder had appeared, laboratory investigations disclosed abnormalities of the humoral and cellular immune compartments in the peripheral blood. This rare observation of gynecologic involvement in a very young girl with family antecedents of Cowden disease prompted a review of the gynecologic features and etiopathogenic bases of Cowden disease. The possible role of neoplasia such as breast cancer in this syndrome makes early diagnosis a criterion for good prognosis.

Adolescent↗
PubMed1989

[Value of immunologic phenotyping of acute leukemias in children].

Immunologic typing has demonstrated considerable heterogeneity among the acute leukemias. The most significant recent advance has been development of monoclonal antibody techniques. Some markers identified using these techniques seem to be specific for a given stage of maturation of one lymphoid or myeloid cell line. Most acute lymphoblastic leukemias (ALLs) are malignant proliferations whose differentiation appears to have become 'stuck' at one stage of maturation. Results of immunologic typing correlate well with the other clinical and biological data. For prognostic purposes, several patterns can be identified. Among B line ALLs, four varieties have been differentiated, i.e., CD10 negative ALLs, common ALLs, pre-B ALLs, and B ALLs. T ALLs include a broad spectrum of heterogeneous proliferations whose immunologic classification is made difficult by the large number of phenotypes encountered. Among acute myeloblastic leukemias (AMLs), some highly undifferentiated forms have been recognized, by means of immunologic typing, as originating in one of the myeloid cell lines. However, the nosologic and prognostic significance of these studies is less obvious than in ALLs.

Child, Preschool↗
PubMed1988

Immunoglobulin-producing cells in human prostate.

Immunohistological techniques were used to investigate the presence of immunoglobulin-producing cells in human prostate. Surgical samples from 14 patients with prostate hyperplasia were analyzed. Lymphoid infiltration was rarely seen and was in no way comparable with the classical structure of mucosae-associated lymphoid tissue (MALT). Plasma cells were observed in nine cases. They produced predominantly IgA and lambda chains, but noticeable numbers of IgM-secreting cells were also seen. These cells were most numerous in patients with antecedents of infection or prostatitis. These data suggest that the local immune system of the prostate is in relation with the MALT, and can be colonized by MALT-derived cells in response to bacterial stimuli.

Aged↗
PubMed1988

IgA nephropathy and alcoholic liver cirrhosis. A prospective necropsy study.

The incidence of mesangial IgA nephropathy (mIgAN) was investigated in a series of patients with alcoholic liver cirrhosis (ALC). Biologic parameters classically reported in IgAN were assessed in 98 patients, namely hematuria, proteinuria, and serum IgA. An immunohistologic study of the liver and kidney was performed in 33 patients who died during the study. Renal data were compared with those obtained in a matched necropsic series of controls. This study confirmed a global elevation of serum IgA levels in ALC. A possible hepatic origin of these immunoglobulins was supported by the observation of plasma cells in portal spaces in 68% of the patients. Biologic signs of renal disease consistent with mIgAN were observed in 16% of the patients; IgAN was diagnosed in 18% of patients with ALC and 10% of the controls. These data suggest that the incidence of mIgAN in ALC is not different than in the general population.

Adult↗
PubMed1988

[Immunohistology in assessment of the spread of cancer].

The prognosis of cancers is conditioned by tumoral extension. We report a case of a squamous carcinoma of the vulva where pathological examination had been at fault. An immunohistological study performed with monoclonal antibodies revealed a lymph node micro-metastasis. This case emphasizes the importance of estimating the tumoral extension by simultaneous pathological and immunological examinations.

Carcinoma, Squamous Cell↗