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Biomedical subjects

M Brozman

Publications and source records attributed to M Brozman.

At least 91 records · Page 5Linked to original sources
PubMed1980

[The morphology of the systemic anaphylactic reaction to bovine serum albumin and ferritin in rabbits. II. Electron microscopic findings].

The picture of rabbit systemic anaphylactic reaction to bovine serum albumin and ferritin is represented by tiny clusters of blood platelets and basophils in the microcirculation of different organs. Also present are signs of damage caused to the endothelium of different vessles, or to some of the blood cells as well as their subsequent breakdown in the liver and spleen. No signs of ferritin-antiferritin complex formation were found in the microcirculation of the affected animals. The platelet-ferritin relationship is seen as evidence of the degradation role of the platelets rather than of any specific antigen linkage to the surface of target cells. The above described systemic anaphylactic reaction was rather one of the whole organism than local reaction in the target organ even though many of the canges note there are probably alterations of a non-specific shock nature. No pronounced degranulation of basophils or other granulocytes was found despite the fact that a certain degree of degranulation could be observed. Morphological findings in model in vitro experiments cannot always be identified with findings observed in vivo during systemic anaphylactic reaction. Há Cs. Patol., 16, 1980, No. 1, p. 5-11.

Anaphylaxis↗
PubMed1979

Effects of feeding a single daily meal and of changes in lighting schedule on the circadian rhythms of oxidoreductases in the rat gastric mucosa.

The present paper investigated the effect of restricted feeding (11.00 to 13.00 h) by a single daily meal upon the activity of various oxidoreductases of the rat gastric mucosa (SDH, alpha-GPDH, LDH, NADHTR), which at ad libitum feeding under normal lighting conditions with a dark night were shown to have distinct circadian activity variations related to the rat's nocturnal food intake preference (Zaviacic and Brozman 1978a). 1st part of the animals received food in 2-h-period by natural daylight (natural lighting conditions), the 2nd part at the same period of time but in the dark (lighting conditions reversed to natural). Checking the consumption of food, we found the animals to become adapted to the new time of food intake beginning with the 6th experimental day. The circadian rhythm of enzymes was examined on days 69, 83, 97, and 104 of limited feeding under the defined lighting schedule, at 06.00 h, 12.00 h, 18.00 h, and 24.00 h in groups of 5 animals. Synchronization of the highest dehydrogenases activities (particularly of SDH) with the time of restricted feeding was seen to develop in the gastric mucosa of the rat. The synchronization effect was more pronounced in the animals which received their food in the dark (and here even more in male than in female). The effect of light during food intake was found to be rather interfering with the development of synchronization of dehydrogenase activities with the time of food intake, and the influence was again more marked in male than in female. The time of food intake was at the feeding schedule restricted to daytime intake the main synchronizer for the circadian rhythm of oxidoreductases of the gastric mucosa of the rat, with the highest daily activities recorded around the feeding time and the lowest activities at night, which is the direct opposite of the circadian rhythm of the same enzymes determined in rats fed ad libitum under normal lighting conditions with a dark night.

Animals↗
PubMed1979

[Immunofluorescence investigation of formol-paraffin material].

Immunohistochemical methods were employed for the investigation of bioptic and necroptic material fixed in formol and embedded in paraffin after it had produced very little or no fluorescence at all in the use of routine immunofluorescence techniques. Partial digestion with pepsin or trypsin or both enzymes in succession mostly facilitated positive immunohistological reaction. This particular procedure gives more scope to the methodological possibilities of histological material examination in that it offers an opportunity for the immunohistological investigation of even old material not specially preserved for immunohistological purposes but routinely fixed in ordinary formol. The simplification adds general availability to the method.

Fixatives↗
PubMed1979

Morphologic changes in the gastric mucosa of rats after administration of N-methyl-N'-nitro-N-nitrosoguanidine. Electronmicroscopic study.

Electron microscopic findings of the effects of exposure to the cancerogenic substance N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) upon the gastric mucosa of rats are presented. MNNG was administered in drinking water at a concentration of 83 mg/l over a period of eight weeks. The resulting findings included occurrence of myelin figures in membrane structures of epithelial cells, occasional disappearance of nuclear membrane, numerous intranuclear cytoplasmic pseudoinclusions, and increased number of perichromatin and interchromatin granules and of nuclear corpuscles. Narrowing of the layer of peripheral condensed chromatin and broadening of the layer of dispersed chromatin were frequently observed. In the cytoplasm of epithelial cells, production of autophagous vacuoles was detected in differentiated types of cells, the presence of great amount of polysomes and profiles of rough endoplasmic reticulum diversely deformed was seen in the undifferentiated neck cells. These cells presented a marked proliferative activity. Some of the changes described in the nuclei of epithelial cells were also observed in different mesenchymal elements in the lamina propria mucosae. The demonstrated changes are indicative of both regressive and progressive effects of the cancerogen used in our experiment.

Animals↗
PubMed1979

[Morphology of systemic anaphylactic reaction in rabbits to bovine serum albumin and ferritin. I. Manifestations of the reaction after antigen administration: laboratory, macroscopic and optical microscopic findings].

A report is presented on systemic anaphylactic reaction [SAR] of rabbits to bovine serum albumin and ferritin. Active anaphylactic sensibilization with a high yield of sensibilized animals was achieved by administering antigens to rabbits aged a few hours up to 3 months. Tre rabbits showed no presence of precipitins in the circulation, but there were reagins against bovine serum albumin or ferritin. There were various types of SAR activity. Macroscopic findings showed acute emphysema and signs of right heart overload. Apart from acute pulmonary emphysema, histology showed occasional bronchospasm and certain manifestations of RES activation as there was evidence of oedematous infiltration of the peribronchial and perivascular spaces. The above morphological findings cannot as yet be regarded as pathognomonic for SAR.

Anaphylaxis↗