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Biomedical subjects

M Brice

Publications and source records attributed to M Brice.

At least 37 records · Page 2Linked to original sources
PubMed1986

Only adult hemoglobin is produced in fetal nonerythroid x MEL cell hybrids.

In order to test whether the ontogenetic origin of human parental cells influences the expression of globin genes, we did fusions of mouse erythroleukemia (MEL) cells with fetal or adult nonerythroid cells and we assessed globin expression in hybrids. For selection of hybrid clones we used a monoclonal antibody that recognizes a human chromosome 11-linked cell surface marker. Globin expression was assessed with fluorescent antibody labeling, globin isoelectric focusing, and S1 nuclease mapping. Chromosome 11-containing hybrids from human nonerythroid cells (adult or fetal lymphoid cells; fetal fibroblasts) produced human adult (beta) but not fetal (gamma) globin chains. These results suggest that the MEL x nonerythroid cell hybrids express the adult human globin independent of whether the human cells derive from the fetal or from the adult stage of human development. Our findings, together with previous observations in MEL x fetal erythroid or MEL x HEL hybrids, show that the globin program of the parental human cell is the main determinant of the expression of fetal human globin in these hybrids.

Chromosomes, Human, Pair 11↗
PubMed1986

[Early excision and grafting in facial burns].

Early excision and graft surgery is now a routinely used procedure, but whereas its application for burns of the hand has been well determined it is employed less extensively for facial lesions. It is a heavy and very hemorrhagic surgery requiring close cooperation between surgeons and intensive care physicians. Its use for the face is not easy to define, difficulties arising because of the need to establish the depth of the burn, the hemorrhagic risk involved and the need for effective compression after graft surgery.

Anesthesia, General↗
PubMed1985

A new compound osteo-myocutaneous free flap: the posterior iliac artery flap.

A hitherto undescribed osteo-myocutaneous free flap is presented. It is based on a branch of the superficial branch of the superior gluteal artery and includes the posterior iliac crest, the posterior part of the gluteus maximus and medius muscles and the overlying skin. This type of flap has allowed us to reconstruct a bony defect of up to 8 cm in the tibia.

Adolescent↗
PubMed1985

[Protocol of mandibular reconstruction].

Conventional methods for reconstruction of the mandible require an adequately vascularized receiving bed and total absence of cutaneous and mucosal fluid loss. These conditions are rarely attained during immediate reconstructive surgery and secondary operations are very often of doubtful outcome because of previous infection. A part from the treatment of small post-traumatic loss of substance by means of iliac or costal bone grafts, it is suggested that free osteo (musculo) cutaneous grafts be used for other cases. The flap preferred is one from the anterior iliac crest pediculated on the deep iliac circumflex vessels.

Bone Transplantation↗
PubMed1984

GM 58/8: a monoclonal antibody that identifies a new lineage-specific determinant expressed by myeloid progenitors (CFU-GM) and their progeny.

A cytotoxic (IgM) monoclonal antibody (McAb) raised against human erythroleukaemia cell line HEL appears specific for the myelomonocytic lineage. Indirect immunofluorescence and fluorescence-activated cell sorter analyses of peripheral blood and bone marrow cells showed that this McAb (designated GM 58/8) reacts exclusively with more than 90% of the granulocytes, monocytes and myeloid precursors. The positive (fluorescent) fraction sorted from McAb-treated bone marrow buffy coat cells showed a marked enrichment of myeloid precursors, while nonmyeloid cells were recovered in the negative fraction. GM 58/8 specifically inhibited the growth of 90-100% of the committed myelomonocytic progenitors (CFU-GM) in five complement-mediated cytotoxicity experiments. GM 58/8 had virtually no effect on the erythroid progenitor (BFU-E and CFU-E) growth. Cell sorting experiments using this McAb resulted in recovery of 84% of CFU-GM among the fluorescent fractions. Comparisons with other anti-myelomonocytic McAbs reported so far suggest that GM 58/8 has more restricted reactivity and shows more efficient CFU-GM cytotoxicity. Patterns of reactivity with normal cells and established lines indicate that the antigenic determinant identified by GM 58/8 is different from that recognized by previously described anti-myelomonocytic McAbs. McAb GM 58/8 could be used to isolate or remove pure populations of progenitors and/or more differentiated cells of the myelomonocytic lineage for in vitro studies. This study also provides evidence that the HEL cell line, derived originally from an erythroleukaemia patient, expresses an antigenic determinant shared by the normal cells of myelomonocytic lineage.

Antibodies, Monoclonal↗
PubMed1984

Anti-HEL cell monoclonal antibodies recognize determinants that are also present in hemopoietic progenitors.

The characteristics of nine monoclonal antibodies (MoAbs) produced using the uninduced cells of a human erythroleukemia line (HEL) as immunogen are described. These antibodies were grouped into four categories by their differences in recognition of normal cells and cells of hemopoietic cell lines. The four MoAbs of group A recognize determinants that are expressed in a large proportion of normal bone marrow cells and other mature cells. The two MoAbs of group B (53/5, 53/6) and the two MoAbs of group C (54/23, 54/39) recognize small proportions of bone marrow cells, whereas the single MoAb of group D (53/10) essentially recognizes only HEL cells. Competition experiments revealed two pairs of competing Abs (53/5 and 53/6; 54/23 and 54/39). In complement-dependent cytotoxicity of progenitors, 53/6 produced 90%-100% inhibition of CFU-E, BFU-E, and CFU-C growth; 54/39 30%-60% inhibition of BFU-E and CFU-C growth; 53/10 produced a variable degree of inhibition of CFU-E and BFU-E. Cell sorting using 53/6 resulted in approximately a 10-12-fold enrichment of CFU-E, BFU-E, and CFU-C among the positive cells. Cell sorting with 54/23 resulted in recovery of over 90% of BFU-E and 100% of CFU-C among the 23.5% of sorted cells showing strong or intermediate positivity. These findings suggest that HEL cells possess surface characteristics that are expressed in several classes of hemopoietic progenitors.

Animals↗
PubMed1984

Monoclonal antibodies detecting antigenic determinants with restricted expression on erythroid cells: from the erythroid committed progenitor level to the mature erythroblast.

Two new cell surface antigens specific for the erythroid lineage were defined with cytotoxic IgM monoclonal antibodies (McAb) (EP-1; EP-2) that were produced using BFU-E-derived colonies as immunogens. These two antigens are expressed on in vivo and in vitro derived adult and fetal erythroblasts, but not on erythrocytes. They are not detectable on resting lymphocytes, concanavalin-A (Con-A) activated lymphoblasts, granulocytes, and monocytes or granulocytic cells or macrophages present in peripheral blood or harvested from CFU-GM cultures. Cell line and tissue distributions distinguish McAb EP-1 and EP-2 from all previously described monoclonal antibodies. McAb EP-1 (for erythropoietic antigen-1) inhibits the formation of BFU-E and CFU-E, but not CFU-GM, colonies in complement-dependent cytotoxicity assays. By cell sorting analysis, about 90% of erythroid progenitors (CFU-E, BFU-E) were recovered in the antigen-positive fraction. Seven percent of the cells in this fraction were progenitors (versus 0.1% in the negative fraction). The expression of EP-1 antigen is greatly enhanced in K562 cells, using inducers of hemoglobin synthesis. McAb EP-2 fails to inhibit BFU-E and CFU-E colony formation in complement-dependent cytotoxicity assays. EP-2 antigen is predominantly expressed on in vitro derived immature erythroblasts, and it is weakly expressed on mature erythroblasts. The findings with McAb EP-1 provide evidence that erythroid progenitors (BFU-E and CFU-E) express determinants that fail to be expressed on other progenitor cells and hence appear to be unique to the erythroid lineage. McAb EP-1 and EP-2 are potentially useful for studies of erythroid differentiation and progenitor cell isolation.

Animals↗
PubMed1983

Monoclonal antibodies specific for globin chains.

Six monoclonal antibodies specific for human globin chains are described. They are produced by stable clones obtained by raising hybridomas using cells of mice immunized with either adult or fetal hemoglobin. Characterization of the antibodies included testing against tetrameric human and other animal hemoglobins, isolated hemoglobin chains, and when indicated, cyanogen bromide fragments. Monoclonals 16-2 and 37-8 are beta-chain specific. Antibody 31-2 recognizes an antigenic determinant common to the alpha and beta subunits. Monoclonal 30-3 recognizes determinants best expressed in the alpha 2 beta 2 tetramer. Antibody 45-1 recognizes a determinant common to beta and gamma subunits, while antibody 51-7 is gamma-chain specific. None of the monoclonal antibodies recognizes mouse hemoglobin, and they display significant differences in binding to hemoglobins of various species. The species-specific reactions and the knowledge of the primary structures of globins allowed deductions about the antigenic sites recognized by two of the monoclonals (16-2 and 45-1). These antihemoglobin monoclonal antibodies will provide useful probes for studying hemoglobin expression in vivo and in vitro.

Animals↗
PubMed1981

Mapping of antigenic sites on human haemoglobin by means of monoclonal antibodies and haemoglobin variants.

Monoclonal antibodies specific for human globin chains have been prepared and the following strategy has been applied in delimiting the antigenic sites involved in antibody binding. The structural sites of the human globin subunit that might be recognised by the monoclonal antibody were deduced from comparisons of the primary structures of mammalian globin chains that did or did not react with the antibody. The involvement of individual residues at these specific sites was subsequently tested by reacting the antibody with abnormal human haemoglobins in which there was either a substitution or a deletion of one of the residues in question. The primary structural site recognised by monoclonal antibody HuHb beta 3-2(an antibody that reacts with the adult haemoglobins from man and macaque monkey, but not with those from baboon and mouse) includes the aspartic acid residue at position 52 of the beta-globin subunit.

Animals↗
PubMed1981

[The course of Cockett's disease (author's transl)].

The term of Cockett's syndrome stands for a lower limb dysfunction with venous stasis originating from the left common iliac compression by the right common iliac artery at the pelvic inlet level. For the authors, there are three evolutive stages. -- first stage: a simple compression without any anatomic venous parietal lesion. -- second stage: to the previous, are added left common iliac vein lesions consisting of an inner vascular band formation. -- third stage: final evolution: ilio-femoral thrombosis. Therefore, the diagnosis must be done as early as possible. It lies on the left lower limb phlebography.

Constriction, Pathologic↗
PubMed1980

[Ergotism of therapeutic origin. A report of 4 new cases (author's transl)].

The observations concern ischemic accidents with various localizations (lower limbs, tongue, uterus, intestine, heart, liver) corresponding to 4 observations for which ergot derivatives (ergotamine tartrate) can be responsible. The most typical accidents are localized at the level of limbs and especially of lower limbs. In the greatest part of observations, a specific terrain (puerperium, vasomotor disorders, suspicion of temporal arteritis) can be considered and favouring factors (infection, and especially associated medicinal treatments with triacetyloleandomycin [2 observations] and Doxycyclin [1 observation]). The posologies of ergot alcaloïds were either superior to the limit prescription or normal. The duration of prescription was variable, but generally short. The arteriography confirms a vascular spasm. In an observation with very severe evolution, various ischemic localizations inducing amputation, two intestinal resections and an hemodialysis for lactic acidosis were noted. These ischemic accidents have to be noticed as early as possible in order to start a treatment; the best one seems to be the association of sodium nitroprussiate in intravenous perfusion with peri-dural anesthesia in an antalgic aim.

Adolescent↗
PubMed1980

[Investigation of obliterative arterial disease of the lower limbs, by muscular scintigraphy using thallium (author's transl)].

The behaviour of thallium is similar to that of potassium. It participates in transmembrane exchanges and accumulates within the cells, in particular muscle cells. The subjects being at rest or at the maximum phase of an effort test, an injection of 2 mCi of thallium 201 as given. Quantitative scintigraphies were performed during the minutes following injection. Ten control subjects were studied in this way. Uptake of thallium in the muscle masses of the lower limbs was symmetrical and homogeneous and was more marked after effort. Acute ischemia for a few minutes was obtained using a tourniquet in a volunteer. The ischemic area was clearly seen on the early scintigraphy. There was redistribution of thallium in the later scintigraphic studies. Fifteen patients with obliterative arterial disease underwent scintigraphy at rest and after effort. Good agreement was found between arteriography, measurement of pressures, plethysmography and deficiencies demonstrated by scintigraphy. The effort test, by inducing transient ischemia, was useful in detecting moderate circulatory disturbances.

Adult↗