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Biomedical subjects

M Breckwoldt

Publications and source records attributed to M Breckwoldt.

At least 145 records · Page 8Linked to original sources
PubMed1982

Subcellular metabolism of [3H]-oestrone sulphate in human gestational myometrium.

Human myometrium obtained at caesarean sections from term pregnancies was homogenized and separated into nuclear, mitochondrial, microsomal and cytosol fractions. Purity of the individual fractions was assessed by microscopy and by determination of marker enzymes. Homogenate and subcellular fractions were incubated with [3H]-oestrone sulphate in a concentration of 5 x 10(-10) M after addition of NADPH2 and a coenzyme regenerating system at 37 degrees C. Incubation were terminated after 10, 20, 40, 60, 120 and 180 min. Substrate and metabolites were characterized by repeated chromatographies on Sephadex LH 20 using various solvent systems. Further characterization of the metabolites was achieved by simultaneous chromatography of the isolated compounds and their authentic cold standards. The enzymes involved in the metabolism of oestrone sulphate to oestrone and oestradiol-17 beta are primarily located in the microsomal fraction. After 40 min 71.1%-73.0% was converted to oestrone, while the conversion rate to oestradiol-17 beta was in the order of 1.38-1.90%. Metabolism of oestrone sulphate seen in the other subcellular fractions is probably due to microsomal contamination. The findings presented indicate that human myometrium is capable of converting oestrone sulphate to free oestrone and oestradiol-17 beta under in vitro conditions.

Estradiol↗
PubMed1982

[Comparative sonographic and endocrine studies of gonadotropin-stimulated menstrual cycles].

In 18 cycles of 16 patients the morphological changes following stimulation by human gonadotrophin were followed by ultra-sonography and radio-immunoassays of 17 beta-estradiol and progesterone (HMG). In 9 of 18 cycles hyperstimulation with more than 3 follicles on the day of chorionic gonadotrophin administration were found. When the human menopausal gonadotrophin treatment (HCG) results in the maturation of more than 10 follicles per ovary the estradiol level reaches more than 2000 pg/ml. In 4 of 8 cycles the administration of HCG triggered the hyperstimulation syndrome. In 5 of 18 cycles pregnancy was induced after a follicle of at least 17 mm diameter was found independant of the number and size of concommitant follicles. Because of the high incidence of HMG hyperstimulation ultra-sonographic prediction of a multiple pregnancy is of no practical value. In 6 of 10 treated cycles without success the deformation or unclear margins of the predominant follicle suggested an insufficient stimulation with HMG. For the monitoring of follicular maturation ultrasonography is superior to estradiol determination since only a large number of follicles showed correlation to elevated estradiol levels.

Adult↗
PubMed1982

Interactions between psychosomatic conflicts and gonadotropin secretion.

This present paper represents an attempt to correlate hormonal patterns and psychoanalytical data in 30 patients with secondary amenorrhea. Based on psychoanalytical data and the maximal LH response to GnRH, which were obtained independently, the patients were divided into two groups presenting differing psychosomatic symptomatologies. Patients with severe psychosomatic disease (group A) complaining predominantly of somatic discomfort, exhibited a weak pituitary response to GnRH. Patients presenting mainly mental symptoms were found to be less affected by the psychosomatic disease (group B). The LH responses to GnRH were only moderately impaired in these patients. Seven patients of group A underwent psychotherapy, resulting in a resumption of regular menses in 4 patients. Eight untreated controls of this group who remained amenorrheic exhibited unaltered gonadotropin in levels. All patients of group B being treated by psychotherapy resumed regular menses, while 5 of 7 control patients developed spontaneous menstruation. The progress of sexual maturation in these patients is correlated with increasing pituitary LH response to GnRH. The present study provides evidence that the degree of a psychosomatic disturbance resulting in secondary amenorrhea is reflected by the pattern of plasma LH after GnRH stimulation.

Adult↗
PubMed1981

Distribution of prostaglandin E2 and prostaglandin F2 alpha receptors in human myometrium.

Human myometrium was studied for specific binding of PGE2 and PGF2 alpha. PGF2 alpha-binding was almost undetectable, but specific binding sites for PGE2 with high affinity (KD = 2.7 +/- 0.4 x 10(-9) M were demonstrated. The binding capacity for PGE2 exhibited a topically different distribution pattern with the highest values in the central parts and low to undetectable levels in the cervical region. Binding characteristics were analyzed by receptor kinetics, revealing a homogeneous receptor population. Binding capacity in uteri obtained from post-menopausal women was of the order of 900-940 fmol/mg protein. Oestrogen pre-treatment and pregnancy were associated with a 3-fold reduction of the PGE2-binding capacity.

Cell Membrane↗
PubMed1980

Plasma levels of FSH and LH in patients with gonadal dysgenesis during sequential estrogen and progestogen therapy.

We describe the plasma levels of FSH and LH in ten patients with gonadal dysgenesis during treatment with a low dosage sequential estrogen-progestogen preparation. The daily dose of mestranol ranged from 12.5--50 microgram. Norethisterone was administered from day 16 onwards, the dose ranging between 0.75 and 1.5 mg. It was shown that 25 microgram mestranol was effective in lowering the elevated FSH levels significantly (alpha < 0.001). LH levels remained unaffected. The combination of 25 microgram mestranol and 1 mg norethisterone produced an increase of FSH and LH within 12 h, maximum levels being reached within 36 h after which there was a progressive decline. Low doses of estrogen and progestogen appeared capable of evoking physiological hypothalamic and pituitary responses in patients with gonadal dysgenesis. The doses employed were sufficient to induce breast development, growth of sexual hair, and withdrawal bleeding and were probably not high enough to induce rapid bone maturation and consequent stunting of growth.

Adolescent↗
PubMed1980

The use of two-channel telemetric systems in obstetrics: an ideal monitoring method for oral PGE2 induction of labor.

Our results demonstrate that free mobility in labor has many advantages. Because of the accelerated uterine contractility, the induction-delivery interval is shortened. No unphysiologic baseline pressure alterations were found. The maximal labor amplitudes did not differ from bedside-monitored labor. No FHR-changes occurred and the fetal outcome was excellent. Being able to move about, women are not influenced by the unfamiliar surroundings of modern labor wards. Pain relief and breathing are better than in the recumbent position. The ideal compound to support labor is oral PGE2.

Administration, Oral↗