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Biomedical subjects

M Braend

Publications and source records attributed to M Braend.

At least 19 recordsLinked to original sources
PubMed1991

Restriction fragment length polymorphism of the caprine beta-globin gene cluster associated with the Hb beta-globin variants in Norwegian dairy goats.

Restriction fragment length polymorphism (RFLP) analysis was used to characterize the beta-globin cluster in Norwegian dairy goats. In 122 individuals, different RFLP patterns were detected after BglII and PstI digestion and hybridization with a caprine beta-globin probe. The location of the polymorphic BglII and PstI sites were determined. The different RFLPs were in linkage disequilibrium with each other and with the beta-globin locus as studied at the protein level. A preferential association between certain RFLP haplotypes and beta-globin variants was observed. Polymorphic DNA fragments in the epsilon-globin genes were detected after MspI digestion and hybridization with a caprine epsilon-probe, and eight different band patterns were observed. Correlation between different MspI haplotypes and the beta-globin variants was determined.

Alleles↗
PubMed1990

Characterization by RFLP analysis, of the Caprine beta-globin gene cluster in Norwegian dairy goats.

Restriction fragment length polymorphism analysis has been used to characterize the beta-globin cluster in Norwegian dairy goats. Several polymorphic Taq I restriction sites were identified by double digestions with Bam HI, Hind III, and Eco RI, and restriction maps of the different beta C, beta A and beta F alleles were established. Allelic association between the previously established beta-globin types at the protein level and specific Taq I alleles was found. Furthermore, a preferential association between certain RFLP haplotypes and beta-globin types was observed.

Alleles↗
PubMed1989

Polymorphism of esterases in plasma of foxes.

Plasma samples from 114 arctic foxes and 40 silver foxes have been investigated by use of isoelectric focusing in polyacrylamide gels. At pH ranges 4-6.5 and 4.2-4.9 and after specific staining, arylesterase (AArE) appeared as polymorphic band patterns. These could be explained by three codominant alleles in arctic foxes and two codominant alleles in silver foxes. Segregation figures in families agreed with the genetic theory.

Alleles↗
PubMed1988

Hemoglobin types in Saanen goats and Barbary sheep: genetic and comparative aspects.

By the use of the Immobiline technique at pH ranges 7.0-7.6 and 6.9-7.9, 16 different hemoglobin (Hb) phenotypes were observed in 61 English Saanen goats. They are explained in this breed by a genetic theory of five beta-globin genes (A4, A6, A8, E, and D) and two closely linked alpha-globin loci ('alpha and "alpha) of which the "alpha has a variant allele, provisionally called "alpha X. Family data together with observed and expected Hb frequencies were in agreement with the genetic theory. Among six Barbary sheep there were three Hb phenotypes explained by the occurrence of the beta-chain alleles B and Cna.

Alleles↗
PubMed1988

Haemoglobin polymorphism in Norwegian red cattle.

By the use of isoelectric focusing in polyacrylamide gels and the Immobiline method at pH range 7.1-7.7, three haemoglobin (Hb) phenotypes were observed in Norwegian Red Cattle. Two one-band phenotypes named HbA4 and HbA6 occurred in unequal proportions in their two-band combination type with the cathodal HbA6 being the stronger. In 76 dam-offspring pairs the distribution of phenotypes were in agreement with codominant single gene inheritance. In an unselected material of 140 animals, gene frequencies were HbA4 = 0.94 and HbA6 = 0.06.

Animals↗
PubMed1987

Search for genetic variation in the blood of Norwegian dairy goats reveals a new polymorphism at the Hb beta A locus.

A total of 150 blood samples tested for serum albumin and transferrin and for red cell carbonic anhydrase, phosphoglucomutase, phosphogluconate dehydrogenase, phosphohexose isomerase, nucleoside phosphorylase, acid phosphatase, 'X'-protein and potassium concentration only showed variation at the 'X' protein and nucleoside phosphorylase loci. Isoelectric focusing over pH range 6-8 showed 145 samples to be of haemoglobin type A and 5 type AD. The haemoglobin A type was resolved into further types by separation over pH 6.9-7.5 in Immobiline polyacrylamide gels. A 2- or 4-band pattern was present in 136 of the samples; a genetic hypothesis based on four or more different haemoglobin A variants is proposed. 14 samples had a 3-, 5- or 6-band pattern. It is assumed that these are heterozygous for a variant of the II alpha gene.

Animals↗
PubMed1987

Polymorphism of transferrin and esterase in Alaskan wolves: evidence of close molecular homology with the dog.

By the use of isoelectric focusing in polyacrylamide gels serum samples from 146 Alaskan wolves were studied with regard to transferrin (Tf) and esterase (ArE) polymorphism, comparing the phenotypic band patterns with those of selected Norwegian dogs. The study revealed Tf and ArE polymorphisms in the wolf with phenotypic band patterns being indistinguishable from the corresponding ones in dogs. This suggests the occurrence of the same two common Tf alleles in the wolf as in the dog. In the ArE system the results are consistent with the occurrence of three alleles which also occur in dogs whereas a fourth allele, so far not seen in dogs, is seen in Alaskan wolves.

Alaska↗
PubMed1987

Expression and genetics of caprine haemoglobins.

The expression of haemoglobin (Hb) has been studied in 260 Norwegian Dairy goats by the Immobiline technique at pH ranges 6.7-7.7, 6.9-7.6 and 6.9-7.5. The majority of goats exhibited two- or four-band patterns. In two-band types the average ratio between the anodal and cathodal band was 74:26. PAGE with 8M urea distinguished three phenotypes for the beta chains, proving that the Hb variation described is in the beta chain. Segregation data in 106 complete sire-dam-offspring families agreed with the existence of four beta globin alleles--A2, A4, A6 and A8. Twenty-seven animals had reversed ratios (R) of Hb bands. In two-band phenotypes the average ratio was 36:64. In 15 complete families where one of the parents had reversed ratio, eight offspring received the R type, indicating a simple genetic control. After urea PAGE the R animals all showed the same alpha chain phenotype which differed from that of goats having common ratios of bands. An additional polymorphism appeared in nine animals as three- and five-band patterns which is assumed to be the result of heterozygosity for II alpha and for II alpha and beta globin genes respectively.

Animals↗
PubMed1979

World veterinary manpower: comparative aspects.

The number of veterinarians in relation to livestock units, human population, milk and meat production and gross national product for 93 countries is presented. The structure of veterinary manpower is discussed and information on veterinary involvement with companion animals and public health is given. For 23 countries representing all the continents, the number of livestock units, human population and gross national product per veterinary school are presented.

Africa↗
PubMed1979

Blood groups of Nigerian cattle. Comparative aspects.

Cattle, red cell antigens as determined by a selected battery of 37 reagents characterizing genes at 10 major loci in 101 Zebu, 63 Ndama and 78 Muturu from Nigeria, have been compared with corresponding red cell antigens of a total of 257 Bos taurus cattle in Norway, the Netherlands, France, Spain, Hungary and USA. The Zebu cattle, represented by the three subtypes Gudali, Red Bororo and Shuwa Arab, showed the highest number of red cell factors. Ndama and Muturu cattle had a significantly lower number of blood factors than the Zebu cattle. The lowest number of blood factors were found in European Bos taurus breeds.

Animals↗
PubMed1979

Seasonal variation of bovine anti-J in the tropics.

Close to the Equator at an altitude of 1750 m above sea level a total of 27 cows of various breeds have been studied with regard to the fluctuation of their anti-J naturally occurring antibodies. Over 16 consecutive months the average total score of anti-J varied from a minimum of 20 in October to a maximum of 37 at the end of December. Practically all the sera had the lowest level of anti-J in October. This is about two months after the lowest yearly maximum temperature. It is concluded that gradual changes in temperature can cause variation in anti-J titer, and that the magnitude of the seasonal temperature changes influences the fluctuation of the natural anti-J.

Animals↗