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Biomedical subjects

M Bibbo

Publications and source records attributed to M Bibbo.

At least 163 records · Page 9Linked to original sources
PubMedDate not supplied

Using a CompuCyte Pathfinder to evaluate cytotechnology student diagnostic performance.

OBJECTIVE: To determine whether the Pathfinder Cytology System facilitates comparison of initial student diagnoses to rescreener diagnoses; provides a platform for collection, storage and retrieval of data on student screening performance; and generates a student screening "score." STUDY DESIGN: Using two CompuCyte Pathfinder units networked to a PC server and printer, eight cytotechnology students prescreened 1,224 gynecologic cases and entered their results into the Pathfinder database. Five staff cytotechnologists rescreened the cases and entered their diagnoses. The database containing the initial and rescreen diagnoses were transferred to a modified scoring grid that computed a screening "score" for each of the students. RESULTS: Student diagnoses matched cytotechnologist target diagnoses in 1,107 to 1,224 total cases (90.4%). Of these 1,107 cases, 996 (81.3%) were reported as "within normal limits" (negative) by both student and cytotechnologist, and 111 (9.1%) were target diagnosed as abnormal (atypical squamous cells of undetermined significance [ASCUS] or above) by both student and cytotechnologist. Of 117 remaining cases, 112 (9.2%) were considered minor discrepancies (one-step discrepancy--e.g., benign cell change-reactive vs. ASCUS--favor reactive), and 5 (0.4%) were considered significant discrepancies (two or more diagnostic categories of difference between student and cytotechnologist-within normal limits vs. low grade squamous intraepithelial lesion). The modified scoring grid developed by CompuCyte for this study was able to compute a numerical score for each student. CONCLUSION: Our preliminary assessment indicated that Pathfinder will facilitate evaluation of student performance. The system shows potential for eliminating the "paper trail" and manual dotting required for traditional student evaluation and, with the addition of a scoring program, may be standardized for use in both educational and clinical settings.

Automation↗
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Cellular fixation. A study of CytoRich Red and Cytospin Collection Fluid.

OBJECTIVE: To test a new fixative system (Roche Image Analysis System, Inc. [RIAS]) that may create a background free of blood and blood products, and to determine if the CytoRich Red system has a potential for automation of nongynecologic cytology. We compared the amount of red blood cells, background material and diagnostic ability in 40 bloody specimens prepared using the CytoRich Red system and our routine fixative, Cytospin Collection Fluid (Shandon, Inc.). STUDY DESIGN: Thirty bloody, nongynecologic specimens and 10 fresh FNA surgical specimens were prepared by adding specimen to equal volumes (10 mL) of CytoRich Red Fixative and Cytospin Collection Fluid. After initial centrifugation, the specimens were resuspended and cytocentrifuged using the Cytospin III (Shandon, Inc.) and the Hettich Universal cytocentrifuge. To test system dependency, specimens from each fixative were prepared using the alternate method of cytocentrifugation. Slides from both fixatives were stained, coverslipped and reviewed for the presence of red blood cells, background and diagnostic ability. RESULTS: Of the 40 CytoRich Red specimens, 92.5% (37) had little or no red blood cells as compared to 22.5% (9) of the 40 Cytospin Collection Fluid specimens. Seventy five percent (30) of the 40 CytoRich Red specimens showed reduction of background material in contrast to 15% (6) of the 40 Cytospin Collection Fluid specimens. Diagnostic ability using CytoRich Red was enhanced by the reduction of red blood cells and background material. CytoRich Red performed equally as well with each cytocentrifugation device. CONCLUSION: CytoRich Red reduces red blood cells and background. Nuclear and cytoplasmic stain appear improved. This allows better evaluation of the cytologic features and interpretation of bloody specimens. It is non-system dependent and can be used with any method of preparation. Also, the reduction of background and blood lends itself to adaptation in the automation of nongynecologic cytology.

Biopsy, Needle↗
PubMedDate not supplied

The case for cytologic follow-up after LEEP.

OBJECTIVE: To examine our loop electrocautery excisional procedure (LEEP) biopsy experience to assess the incidence of abnormal follow-up and relate this to margin status. STUDY DESIGN: Records of 162 LEEP procedures performed between January 1992 and April 1994 were reviewed to evaluate margin readability and status. All follow-up cytologic or histologic specimens were examined. RESULTS: After a mean 10.9-month follow-up, of 162 cases, 67 had a negative study (41.4%). Fifty-four (33.3%) had an abnormal result, with high grade squamous intraepithelial lesion (HSIL) in 16 cases (13.2%) of all LEEPs with follow-up, low grade squamous intraepithelial lesions in 27 (22.3%) and squamous atypia in 11 (9.1%). On microscopic review of these 54 cases, 37 had a readable margin (68.5%); of these readable margins, 12 (32.4%) were positive. Forty-one cases (25.3% of all LEEPs) had no follow-up study. CONCLUSION: The high incidence of HSIL after LEEP highlights the need for vigilant follow-up. Prediction of follow-up by margin status is dubious. Thermal artifact makes the margin uninterpretable in many cases, and two-thirds of cases with abnormal follow-up occurred where the LEEP margin was negative. Our finding of no follow-up for 25% of LEEPs performed is particularly disturbing in light of these results.

Artifacts↗
PubMedDate not supplied

Residual ovarian tissue mimicking malignancy in a patient with mucinous carcinoid tumor of the ovary. A case report.

BACKGROUND: Mucinous carcinoid tumor of the ovary is an uncommon lesion in reproductive-age women. If a patient status post total abdominal hysterectomy and bilateral salpingo-oophorectomy (TAH-BSO) for this tumor presents with a pelvic mass, recurrence of the tumor must be considered, as must the presence of residual ovarian tissue producing physiologic cysts with mass effect, termed the "ovarian remnant syndrome." Benign ovarian follicle cysts may have cellular atypia and mimic malignancy. CASE: A female, one year status post TAH-BSO for mucinous carcinoid tumor of the ovary, presented with pelvic mass. Clinical and radiologic evidence supported the diagnosis of recurrent tumor. Aspiration biopsy material was compatible with malignancy, and immunocytochemical stains supported a neuroendocrine origin of the cells. Surgical excision and histologic examination of the mass revealed ovarian tissue with features of corpus luteum and follicular cyst. CONCLUSION: Numerous pitfalls exist when considering an unusual tumor. While the patient's history, clinical impressions and immunocytochemistry may strongly suggest malignancy, more common benign entities may mimic malignancy and should be considered in the differential diagnosis.

Adult↗
PubMedDate not supplied

Clinical utility of chromosome 17 alpha satellite probe in distinguishing benign mesothelium from malignant cells: a pilot study using routinely fixed specimens.

Fluorescent in situ hybridization has shown promise in detecting malignant cells in body cavity effusions. However, this requires special preparatory techniques not used in many laboratories. We developed an in situ hybridization (ISH) procedure specifically for ethanol-fixed specimens, and using it tested the clinical utility of the chromosome 17alpha satellite probe (C17alpha). ISH with C17alpha was used in 12 malignant and 10 benign ethanol-fixed body cavity effusions. Cells were pretreated with protease K prior to ISH. The probe was detected by an anti-digoxigenin-horseradish peroxidase method. Signals were counted in 100 nuclei and the chromosome index (CI) and percent diploid cells calculated in each case. ISH was successfully performed in all cases. Malignant cells had an average CI of 2.23 with less than 44% diploid nuclei and 50% of specimens exhibited bizarre signals. Benign effusions had an average CI of 1.98 with over 84.6% diploid nuclei. Questionably bizarre signals were seen in two (20%) benign specimens. ISH can be performed on ethanol-fixed specimens. The C17alpha probe may prove valuable in detecting malignant cells in body cavity effusions.

Chromosomes, Human, Pair 17↗
PubMedDate not supplied

Cytologic findings in female and male offspring of DES treated mothers.

Data on vaginal, cervical, and endocervical samples and urine specimens of 84 female offspring exposed to diethylstilbestrol in utero and 43 controls have been presented. The colposcopic and histopathologic findings were correlated with the cytologic findings. Our preliminary findings indicate that the cytologic technique is reliable as screening test for vaginal epithelial changes in the DES-exposed females provided the sampling procedure is adequate. In 79 male patients, 42 DES-exposed and 37 controls, the pre-massage urine, the prostatic fluid, the post-massage urine and aspirate of cysts of the epididymis when existing were examined by cytologic techniques. No tumor cells were found in the cytologic specimens of the exposed and unexposed male and female offspring.

Adult↗
PubMedDate not supplied

The TICAS-RTCIP real time cell identification processor.

A preliminary model of a real time processing system, the TICAS-RTCIP (for Taxonomic Intra-Cellular Analytic System--Real Time Cell Identification Processor) has been disigned and is being tested under operational conditions, proving the substantial advantages of employing a network of microprocessors operating in parallel. The system is designed only to demonstrate the feasibility, while more extensive development and biologic testing will be required to assess general applicability to automated uterine cytology.

Computers↗
PubMedDate not supplied

Computer discrimination between benign and malignant urothelial cells.

Computer discrimination between benign and malignant cells by means of the TICAS routines and subroutines has been accomplished with a small error of classification, not greater than ten percent. One feature of major diagnostic significance, namely, nuclear texture was singled out for discussion. This study augurs well for the future of computerized cytology of the urinary tract.

Cytodiagnosis↗
PubMedDate not supplied

The numerical composition of cellular samples from the female reproductive tract IV. Carcinoma in situ cases exhibiting other than normal vaginal flora.

The numerical composition of vaginal, ectocervical and endocervical (VCE) cell samples from patients with carcinoma in situ of the uterine cervix exhibiting other than normal vaginal flora is described. The comparative results of cell counts in patients with carcinoma in situ with normal and other than normal vaginal flora are presented.

Carcinoma in Situ↗