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Biomedical subjects

M Bibbo

Publications and source records attributed to M Bibbo.

At least 181 records · Page 10Linked to original sources
PubMedDate not supplied

The numerical composition of cellular samples from the female reproductive tract. V. Cell cluster patterns in cases of invasive squamous carcinoma of uterine cervix.

One hundred fifty thousand cells in a vaginal, ectocervical and endocervical smears from 25 patients with poorly to moderately differentiated squamous cancer of the uterine cervix were evaluated to determine the numerical composition of cellular clusters, the sizes of the clusters and the cellular types contained. The study is a baseline assessment for the design of automated cytology devices for which monocellular layers or individual cells are of importance. The evaluation indicates that the majority of cells appear in actual or facultative clusters, and that dispersement or breaking up of these clusters can yield improved machine-readable samples.

Carcinoma, Squamous Cell↗
PubMedDate not supplied

Patient classification based on cytologic sample profiles. I. Basic measures for profile construction.

Past efforts in the field of automated cell recognition have focused upon the separation and classification of cell types. From these efforts, large data banks have been built and work in the field is now shifting towards the practical application of this information for clinical diagnoses. This paper presents the initial results of work on a system developed to undertake the reduction of the masses of data into diagnostically useful patient cytologic sample profiles.

Autoanalysis↗
PubMedDate not supplied

Stereotaxic fine needle aspiration cytology of clinically occult malignant and premalignant breast lesions.

Stereotaxic fine needle aspiration (FNA) cytology was used to study clinically occult (nonpalpable) breast lesions in 114 consecutive patients with mammographically suspicious findings prior to excisional biopsy. The aspirate contained insufficient material for cytologic evaluation in 15 cases (13.2%), which were histologically diagnosed as benign (7 cases), atypical hyperplasia (7 cases) or carcinoma in situ (1 case). The cytologic findings indicated a benign lesion in 77 cases (67.5%), which were histologically diagnosed as benign (71 cases) or atypical ductal hyperplasia (6 cases). The cytologic sample showed atypia in eight cases (7.0%), which were histologically diagnosed as severe atypical ductal hyperplasia (three cases), carcinoma in situ (one case) or proliferative fibrocystic disease (four cases). In the eight cases (7.0%) cytologically interpreted as probably malignant, histology confirmed six invasive carcinomas, one carcinoma in situ and one fibrocystic disease. Of six cases (4.4%) cytologically reported as malignant, five were histologically diagnosed as invasive carcinoma and one as carcinoma in situ. Overall, stereotaxic FNA cytology reported as malignant or probably malignant 14 of the 15 cases with a histologic confirmation of malignancy, for a sensitivity of 93.3%. Cytology correctly identified 78 of the 83 histologically negative cases, for a specificity of 94.0%. The 16 cases histologically diagnosed as ductal hyperplasia, which carries a high risk for subsequent malignancy, were studied in detail in an effort to define histologic and cytologic criteria for this entity. Using selected histologic criteria, 11 of these cases were graded as showing mild-to-moderate atypical hyperplasia and 5 as showing severe atypical hyperplasia. Three of the latter cases were similarly identified by an analogous cytologic grading; the other two cases had insufficient cytologic samples. The total results in this series of 114 cases support the use of stereotaxic FNA cytology in the diagnosis of these nonpalpable breast lesions, examples of which are illustrated. In particular, it may help to raise the low specificity yielded by mammography alone, which would represent a significant advance for the patient in terms of the accuracy, expediency and reduced cost of diagnosing these lesions.

Adult↗
PubMedDate not supplied

Bronchial metaplasia as a benign or premalignant lesion. I. Cytologic and ultrastructural discrimination between acute carcinogen effects and toxin-induced changes.

The respiratory tracts of hamsters were exposed to either of two different chemical compounds contained in cigarette smoke, the carcinogenic chemical benzo(a)pyrene or the noncarcinogenic chemical formaldehyde. At various times during or shortly after the exposure, the acute cytologic, histologic and ultrastructural responses to the two agents were compared. Both agents induced microscopically similar squamous metaplastic changes of the tracheobronchial epithelium. However, there were several cytologic and ultrastructural differences in the appearance of the chromatin, nucleoli and cytoplasm that could be used to separate carcinogen-induced from noncarcinogen-induced acute metaplastic changes. Furthermore, after cessation of the exposures, squamous metaplastic changes induced by the noncarcinogenic agent formaldehyde regressed to normal, while the acute carcinogen-induced changes were replaced by other, more persistent metaplastic changes.

Animals↗
PubMedDate not supplied

Computer analysis of atypical urothelial cells. I. Classification by supervised learning algorithms.

Computer discrimination of atypical (ATY) urothelial cells from the urinary sediment by means of supervised learning algorithms discoled that these cells form a distinct, although ill-defined, family of cells which differs from normal (NEG) and malignant (POS) cell groups. The clinical significance of this observation must await long-term clinical follow-up. The possibility of issuing computer displays on individual patients with possible diagnostic and prognostic implications is discussed.

Aged↗
PubMedDate not supplied

Clinical applications for an inexpensive, microcomputer-based DNA-cytometry system.

Some of the clinical capabilities of an inexpensive, microcomputer-based DNA-cytometry system are described. A variety of applications is illustrated: ploidy assessment, which provides significant information for the management of patients with tumors from almost any organ site; quality control of cytologic and histologic diagnoses; determination of tumor heterogeneity; monitoring of cell profile changes during therapy; and quantitation of immunocytochemical staining.

Adult↗
PubMedDate not supplied

Dimensionality reducing displays in cell image analysis.

The paper discusses the problem of representation of multivariate cell data sets in two dimensions such that the essence of the situation as represented by the multivariate feature space is preserved. A corresponding projectional technique has been developed and illustrated on a set of five different cell types of ectocervical cells, including normal and carcinoma cells.

Computers↗
PubMedDate not supplied

Computer recognition of ectocervical cells. Classification accuracy and spatial resolution.

The effect of a relaxation of the spatial resolution of images of ectocervical cells on classification accuracy by computer has been studied. The discriminatory capabilities of optical density, texture and shape features have been evaluated. Comparable computer classifications have been derived. The differences in the discriminating power of .5 and 1 mu resolution are found to be smaller than the differences corresponding to 1 and 2 mu resolution. While 2 mu resolution may be marginally acceptable in certain situations, the 4 mu resolution did not render acceptably good classification rates.

Computers↗
PubMedDate not supplied

Computer recognition of abnormal ectocervical cells. Comparison of the efficacy of contour and textural features.

The efficacy of contour and texture features for the computer recognition of abnormal ectocervical cells is compared. For this comparison, three cell groups were formed: benign, suspicious and malignant. Nine decision rules were derived. The discrimination attained by features derived from the contours of nucleus and cytoplasm is comparable to that achieved by textural features. A combination of both kinds of features gives best results.

Cell Nucleus↗
PubMedDate not supplied

Computer recognition of binucleation with overlapping in epithelial cells.

A technique for computer recognition of binucleation with overlapping in epithelial cells was derived. The computer program uses analytic features related to nuclear contour only. The feature set includes moments, Fourier transform features and linguistic features. The algorithm works satisfactorily in instances where the human eye would detect the presence of two nuclei at first glance.

Cell Nucleus↗
PubMedDate not supplied

A system for scanning biological cells in three colors.

A system for scanning biological cells under high magnification has been developed which utilizes a three-color photometer. The spectral information has proved useful in improving computer recognition of certain cell types.

Cytodiagnosis↗
PubMedDate not supplied

An automated device for the production of cell preparations suitable for automatic assessment.

A set of preparatory steps has been developed for the processing of clinical samples from the female genital tract onto microscopic slides suitable both for machine assessment and for regular microscopic evaluation. These steps involve the depositing of the cell sample in a collection fluid, the semi-automatic adjustment of cell concentration to about 30,000 cells per milliliter through a combination of transmittance and light scatter measurements, the dispersion of the sample by syringing, and the deposition of the cellular content onto microscope slides by centrifugation. These steps were designed so as not to allow loss of cells--except the lysis of erythrocytes--with the understanding that this condition entails the presence of 4--10 per cent cell aggregates in the final preparation.

Autoanalysis↗
PubMedDate not supplied

Automated hierarchic decision structures for multiple category cell classification by TICAS.

TICAS-PROBE, a computerized system has been developed to generate hierarchic decision structures with little human intervention. It has been tested with several tasks, and the results are comparable, if not better, than those produced by human designers. Additional methods of generating the individual elements will be included in the near future.

Autoanalysis↗