Biomedical subjects
M Bailly
Publications and source records attributed to M Bailly.
[Swollen legs of arterial and mixed origin].
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[Mixed dacron-vein bypass. Apropos of 33 cases].
The use of a mixed dacron-vein by-pass is a technical device permitting one to palliate the impossibility of using exclusively venous material. 33 by-passes were thus carried out:--30 distal by-passes;--3 proximal by-passes. The interest of this technique is the possibility of implanting on a small caliber artery, e.g. profunda femoris, popliteal or femoral artery. The main technical point is the immediate anastomosis. Since this article was drawn-up, the indications for this procedure have been multiplied and, over a period of six months, we carried out 32 new mixed by-passes.--27 proximal by-passes;--5 distal by-passes.
Direct enzymatic determination of urea in plasma and urine with a centrifugal analyzer.
A direct enzymatic micromethod (sample volume, 3mul) has been adapted to the centrifugal analyzer (ENI-GEMSAEC) for measurement of urea in plasma and urine. The method is based on urease (urea amidohydrolase, EC3.5.1.5)/glutamate dehydrogenase [l-glutamate:NAD(P)+oxidoreductase (deaminating), EC1.41.3] coupled reactions, and uses a two-point fixed-time (t(1)=20s,t(2)=50s)kinetic scheme for monitoring the rate of comsumption of NADH at 340 nm. Sensitivity and precision of the method are excellent,and results compare well with those from a commonly used continuous-flow method.
[Experimental study of the possibilities of liver preservation for 24 hours and longer under hypothermia, hyperbaric oxygen and perfusion. II. Biochemical results].
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Clonal drift and role of chromosome dosage in human melanoma metastatic cell lines: a statistical analysis.
Karyotypic analyses were performed on twenty human melanoma clones and variants all deriving, following in vivo selections and/or in vitro cloning, from the parental M4Be cell line, but expressing different tumorigenicity (expressed as mean tumor weight) and metastatic ability (expressed as pulmonary metastasis frequency) after s.c. injection in antithymocyte immunosuppressed newborn rats. These cells were hypertriploid, showing quite comparable modal numbers (around 70). They all expressed a wide range of chromosome number per mitosis (28 to 198), as well as a large extent of karyotypic heterogeneity, showed by extensive clonal drifts within the different cell lines. Their common origin was ascertained by five clonal abnormal marker chromosomes deriving from chromosomes 6, 7, 8, 9, 11 and 14. Twenty-one additional marker chromosomes, most of them non clonal, were observed in the different cell lines. We developed a statistical analysis to search for putative relationship between the expression of tumorigenicity and metastatic ability and the evolution of specific subclones within the different cell lines. We showed that the expression of a high mean tumor weight and/or a high metastasis incidence was related to the modification of the ratio of different subclones within each cell line, and more especially to the emergence of subclones presenting partial losses of individual chromosomes or chromosomal fragments, which were encountered with a lower frequency in the low metastatic cell lines. The biological relevance of these findings in terms of clonal evolution and role of chromosome dosage in tumor progression is discussed.
Metastases of human tumors in experimental animals.
A large variety of models of both artificial and spontaneous metastases have been developed in experimental animals. These models have enabled the characterization of metastatic cancer cells and have helped in understanding the metastatic process. Studies of experimental metastases of human tumors have so far been rather limited; these have been developed by xenografting human tumors in immuno-compromised animals, especially athymic nude mice. Although nude mice only seldom develop metastases when grafted with human tumor cells, the selection of human cancer cells with increased metastatic abilities could be obtained in a number of cases. Human melanoma variants and clones with increased metastatic abilities were obtained from melanoma cell lines in nude mice and in immunosuppressed newborn rats. Subcutaneous transplantation in a nude mouse of a human melanoma metastatic nodule resulted in a subcutaneous tumor (NTT) and in spontaneous lung (NTP) and lymph node (NTG) metastases which were first maintained in vivo by subcutaneous passages in nude mice and then cultured in vitro as cell lines. Cytogenetic studies showed that all three tumor lines have a common origin and that metastases resulted from a population selection. After 15 in vitro passages, NTP cells were reinjected s.c. in nude mice: serial transplantation was accompanied by an increase in metastatic abilities of tumor cells. Melanoma cell lines, tumorigenic but non metastatic in nude mice were xenografted to ATS-treated newborn rats. 3 weeks after s.c. injection of 10(6) cells, nearly all rats developed tumors and a proportion of them lung and lymph node metastases. Agar cloning of M4Beu line showed that it is heterogeneous and contains poorly tumorigenic but highly metastatic cells. In addition, serial in vivo passages resulted in the selection of highly tumorigenic but poorly metastatic cells.(ABSTRACT TRUNCATED AT 250 WORDS)
[Arteritis of lower limbs and local muscular output with xenon 133. Presentation of an isotopic method of vascular exploration].
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[Ecology of leishmaniasis in the south of France. 11. Canine leishmaniasis: successful experimental transmission from dog to dog by the bite of Phlebotomus ariasi Tonnoir, 1921 (author's transl)].
As part of a study of visceral leishmaniasis in the Cévennes in southern France, an infection was transmitted from dog to dog by the bite of a single sandfly, Phlebotomus ariasi Tonnoir, 1921. The role of this species as a vector, suspected from earlier studies, is, therefore, confirmed. Twenty female sandflies, which had engorged on a naturally infected dog 23 +/- 2 days previously were put with a healthy dog which, after an incubation period of 15 months, developed viscero-cutaneous leishmaniasis. In dissections of the sandflies immediately after contact with the experimental dog, it was found that only one had engorged. All of 17 sandflies dissected had midgut infections: 10 of these also had infections in the pharynx. The engorged specimen additionally had parasites in the proboscis, a condition thought by many workers to be necessary for the parasite to be transmitted by bite.
[Ecology of leishmaniasis in the south of France. -- 12. Horizontal dispersion of Phlebotomus ariasi Tonnoir, 1921. Preliminary experiments (author's transl)].
By use of mark-release-recapture methods, evidence was obtained of the distance of the dispersion of Phlebotomus ariasi Tonnoir, 1021. Female flies, collected in a valley in the Cévennes mountains (at Roquedur, Gard), were marked with fluorescnt powders, released an then recaptured by searching with ultra-violet lamps. The majority of the flies recapture were among ones given a blood meal shortly before release. Recaptures were made from the second day after release. A number of females migrated with blood in the midgut; their ovaries were up to and including stage V. The distance between the extreme points of recapture was about 1 km, and the maximum recorded distance between points of release and recapture was 750 m. The epidemiological interest of these observations is that, at least in the Cévennes foci, the sandfly as well as the dog must now be considered as capable spreading the pathogenic agent of visceral leishmaniasis.
[Control of accuracy in hemacytometry based on the daily average of patients' tests. A rational method by simulating the detectable error (proceedings)].
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[Supervision of thyroid function during prolonged treatment with amiodarone (author's transl)].
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Human melanoma metastasis related to specific adhesion with lung cells rather than direct growth stimulation.
We previously established a model to study human tumor spontaneous metastasis in immunosuppressed newborn rats. Different observations suggested that the development of pulmonary metastases of human melanoma cells in this animal might reflect, at least partially, an organ specificity phenomenon. We thus examined the "soil" property of the rat lung in terms of growth stimulating activity and specific interactions between tumor cells and pulmonary cells. We could not demonstrate any melanoma cell growth-promoting activity in lung conditioned medium. On the other hand, tumor cell adhesion was drastically enhanced when measured on pulmonary fibroblast monolayers or derived extracellular matrix. Adsorption of lysates of 7GP122 highly metastatic melanoma variant on viable total lung cells enabled us to detect at least 10 proteins or protein subunits which could specifically interact with pulmonary cell membranes, while only one of these proteins was detectable when the same experiment was performed with control liver cells. Conversely, we could show that there exist several corresponding structures on pulmonary cells which could adsorb on tumor cells. Thus, specific cell surface adhesion molecules, leading to specific adhesion between melanoma cells and pulmonary cells, may lead to preferential metastatic development in rat lung.