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Biomedical subjects

M Bailey

Publications and source records attributed to M Bailey.

At least 217 records · Page 12Linked to original sources
PubMed1984

Haematological and biochemical values in horses naturally infected with Strongylus vulgaris.

The concentrations of serum proteins (beta 1, beta 2, gamma, alpha 1, alpha 2 globulins and albumin) and absolute numbers of eosinophils, neutrophils and lymphocytes were examined in 64 naturally infected horses and ponies in which the number of larvae of Strongylus vulgaris in the cranial mesenteric artery and the severity of the lesion of verminous arteritis could be determined. The horses were grouped according to the number of larvae found and the severity of the arteritis. The results demonstrated that, although some significant deviation from a random distribution occurred in certain of the values (chi 2 test), there was considerable individual variation in the values obtained for individual animals within groups and overlap of the range of values between groups. Also the number of larvae present in the artery did not necessarily accurately reflect the severity of the arterial lesion. Thus, the parameters examined could not be used reliably to estimate the intensity of infection with S vulgaris in an individual animal.

Animals↗
PubMed1984

In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen.

Proliferation in vitro of peripheral blood lymphocytes both from horses infected with Strongylus vulgaris and from helminth-free ponies was observed in the presence of extracts of the fourth and fifth stage larvae and adults of S. vulgaris. In addition, S. vulgaris extracts induced transformation in cultures of peripheral blood lymphocytes from sheep and dogs and in mouse spleen cell cultures. Nylon wool non-adherent, T cell enriched fractions of lymphocytes from both mice and horses were stimulated by the S. vulgaris larval mitogen while no proliferation was observed in cultures containing nylon wool adherent, B cell enriched fractions. Macrophage co-operation appeared not to be necessary for S. vulgaris mitogen-induced transformation of spleen cells. The S. vulgaris mitogen stimulated a subpopulation of mouse spleen cells different from those responsive to PHA, Con A and LPS. These cells might be T helper cells since B cells were stimulated to proliferate in the presence of both T cells and S. vulgaris larval mitogen. In addition, the supernatant of in vitro cultured larvae of S. vulgaris induced slight, but significant transformation of equine peripheral blood lymphocytes. Therefore, it is possible that the S. vulgaris mitogen released by both viable parasites and degenerating larvae might induce T cell dependent production of immunoglobulin in vivo and account for the beta-globulinaemia, of which IgG(T) is a major component, in S vulgaris infected horses.

Animals↗
PubMed1984

Use of isoelectric focusing of bacterial proteins for characterisation of strains of motile anaerobic "corroding" bacteria isolated from soft tissue infections in cats and dogs.

Isoelectric focusing of bacterial proteins in 1 mm thick agarose gels and ultrastructural analysis of cell walls were used to facilitate characterisation of motile "corroding" bacteria from subcutaneous abscesses and pyothorax infections from cats and dogs. Some of the motile "corroding" rods had ultrastructural cell wall features identical with Wolinella recta described from human periodontal pockets. Other strains had the more conventional cell wall ultrastructure of Gram negative bacteria. Isoelectric focusing of bacterial proteins divided the motile "corroding" strains into two groups. These groups correlated with the ultrastructural appearance of the cell walls. It is considered that isoelectric focusing techniques using 1 mm thick agarose gels may provide a more rapid method for ultimate differentiation of these organisms than other methods described so far.

Animals↗
PubMed1984

Postoperative analgesia for Caesarean section using epidural methadone.

A prospective randomised double blind study was carried out to compare the use of epidural methadone, morphine and bupivacaine for pain relief after Caesarean section. The results indicate that methadone is the most effective agent with few side effects. Subsequently this method was used routinely for postoperative analgesia in all patients undergoing Caesarean section. A retrospective study of 178 patients having this method of analgesia was carried out and indicated that epidural methadone is an effective and safe method of postoperative pain relief.

Anesthesia, Epidural↗
PubMed1984

Comparison of strains of gram-negative, anaerobic, agar-corroding rods isolated from soft tissue infections in cats and dogs with type strains of Bacteroides gracilis, Wolinella recta, Wolinella succinogenes, and Campylobacter concisus.

A total of 64 strains of gram-negative, asaccharolytic, anaerobic, agar-corroding, rod-shaped bacteria from soft-tissue infections of cats and dogs were compared with other agar-corroding, anaerobic organisms isolated from human periodontal pockets (Wolinella recta ATCC 33238T), bovine rumens (Wolinella succinogenes ATCC 29543T), and gingival crevices of humans (Bacteroides gracilis ATCC 33236T and Bacteroides ureolyticus NCTC 10941T). Campylobacter concisus ATCC 33237T (from human gingival crevices) which did not corrode agar but which biochemically resembled organisms in this group was also included in this study. Although the type strains of W. recta, W. succinogenes, and B. gracilis resembled the animal strains phenotypically and in DNA base ratios, none had bacterial protein patterns (as indicated by isoelectric focusing) identical with the animal strains studied. The animal strains could be divided into motile and nonmotile groups. The motile animal strains were similar biochemically but could be divided into three groups by isoelectric focusing of bacterial proteins. Some had cell wall ultrastructural features identical with W. recta; others had the smooth walls of conventional gram-negative organisms. One group of nonmotile animal strains closely resembled B. gracilis phenotypically, and they had the cell wall ultrastructure of conventional gram-negative bacteria as described previously (4). The other nonmotile group had cell wall ultrastructure like that of W. recta.

Agar↗
PubMed1983

Serum concentrations of penicillin in the horse after administration of a variety of penicillin preparations.

Twelve geldings were used to evaluate serum levels of penicillin following the intravenous (iv) and intramuscular (im) injection of benzylpenicillin, im procaine penicillin and im fortified benzathine penicillin, at a variety of dose rates. Blood samples were collected up to 7 h after iv and im benzylpenicillin, up to benzathine penicillin. Results were analysed using an analysis of variance and regression analyses were used to calculate the time taken for the serum penicillin concentration to decline to 0.5 micrograms/ml. As a result of the experiments the following dosage regimes could be recommended for each drug. Benzylpenicillin should be administered im at a dose rate of 10,000 iu/kg at the commencement of treatment. Procaine penicillin should be injected at a dose rate of 15,000 iu/kg every 12 h or 30,000 iu/kg every 24 h. In serious infections 10,000 iu/kg of benzylpenicillin im should be used concomitantly to begin therapy. Fortified benzathine penicillin appears to have little value for antimicrobial therapy in the horse.

Analysis of Variance↗
PubMed1982

Life after hospital: trials of a boarding house 'mother'.

What is life like for the many psychiatric patients discharged from hospital to boarding houses? The 'mother' of a Sydney boarding house describes how her 'family' has taken root, grown, developed and made a stable home. Many behaviours not tolerated in the community (e.g. public masturbation) are acceptable in the home but there are limits and rules (e.g. not in the dining room). A good home needs the responsible person to be open, honest, understanding and sensitive to changes in guests. Psychiatric services and welfare agencies need to recognise the major contributions of well-run boarding houses and need to liaise more with the proprietors.

Adolescent↗
PubMed1981

Serum levels of amoxycillin following its oral administration to thoroughbred foals.

Amoxycillin trihydrate was administered orally to 6 foals at dose rates of 13 mg/kg (low), 20 mg/kg (medium) and 30 mg/kg (high) and serum concentrations determined at intervals up to 8 h. Therapeutic serum levels of 1 microgram/ml persisted for 268 mins at a dose rate of 13 mg/kg, for 339 mins at 20 mg/kg and for 381 mins at 30 mg/kg. A 2 micrograms/ml serum level persisted for 198 mins at a dose rate of 13 mg/kg, for 268 mins at 20 mg/kg and for 311 mins at 30 mg/kg. To determine the spectra of its antibacterial activity, the minimum inhibitory concentrations of amoxycillin against 8 genera of common pathogenic bacteria in foals were examined. Of the bacteria tested, only Streptococcus equi, Strep zooepidemicus and Staphylococcus aureus (non-beta lactamase producing) would have been treated effectively with the serum levels which persisted in these foals. It is concluded that amoxycillin cannot be considered for broad spectrum use against pathogens likely to be encountered in the foal, but, because of its ease of administration orally, has a useful role in treating streptococcal infections and staphylococcal infections not caused by beta lactamase producers. If sensitivity testing of isolates is made, the drug may be useful also for treatment of some infections caused by the Gram-negative organisms Actinobacillus equuli and Salmonella typhimurium.

Administration, Oral↗
PubMed1979

Description of strains of Peptostreptococcus anaerobius isolated from subcutaneous abscesses in cats.

Strains of Peptostreptococcus, Streptococcus and of a Gram-positive coccus, which was initially isolated as an anaerobe but grew subsequently as a facultative organism, were isolated from subcutaneous abscesses in cats. The cat strains of Peptostreptococcus gave metabolic fermentation products in combinations described for P. anaerobius. The Streptococcus strains conformed to the group S. intermedius. The facultative organism described had the metabolic products of P. anaerobius but the distinctly different biochemical characteristics of S. intermedius and fits neither of the genera strictly.

Abscess↗
PubMed1979

Isolation and characterisation of bacteria from abscesses in the subcutis of cats.

Thirty-six closed abcesses in the subcutis of cats were examined. Of 168 bacterial strains isolated, 121(72%) were anaerobes and 47 (28%) were facultative anaerobes. Twenty-six abscesses contained mixtures of facultative anaerobes and anaerobes, six contained anaerobes only and four contained facultative anaerobes only. Bacteriodes was the genus most commonly isolated (28.6% of all isolates) followed by Fusobacterium (19.0%) and Pasteurella (multocida) (13.1%). Peptostreptococcus anaerobius was the most commonly isolated anaerobic species (13.2% of anaerobic isolates and 9.5% of all isolates)and Past, multocida was the most commonly isolated facultative anaerobe (46.8%; 13.1%of all isolates).

Abscess↗