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Biomedical subjects

M Andre

Publications and source records attributed to M Andre.

103 records · Page 6Linked to original sources
PubMedDate not supplied

Persistent inhibition of neutrophil function by glucose based dialysis solutions.

Local defense mechanisms play an important role in prevention of peritonitis, a major complication of continuous ambulatory peritoneal dialysis (CAPD) therapy. The authors have shown that hypertonic, lactate containing glucose based dialysis solutions (GBDS) used in CAPD lead to an immediate and complete pH-dependent inhibition of actin polymerization and phagocytosis in polymorphonuclear neutrophils (PMN) in vitro. Earlier studies have shown that the pH of the fluid equilibrates from 5.2 to approximately 6.4 during the first 30 min of intraperitoneal dwell time. Thus, the authors designed the current study to determine whether the inhibition of cytoskeletal function and intracellular acidosis induced by acidic solutions are reversed by this pH adjustment. To this end, actin polymerization, phagocytosis, and intracellular pH were studied in PMN isolated from healthy human donors during a 10 min incubation in commercially available GBDS at pH 5.2 and again after pH adjustment to 6.4. Actin polymerization was assessed by measuring F-actin content using NBD phallacidin staining and fluorescence activated cell scanner analysis. Phagocytosis was assessed using zymosan particles, and intracellular pH was monitored by spectrofluorometry. The impairment of cytoskeletal alterations in cells exposed to GBDS at pH 5.2 was persistent and not fully reversed by adjusting the pH. Likewise, phagocytosis remained markedly inhibited and intracellular pH did not rise after adjustment of pH. Thus, the results demonstrate a persistent cytotoxic effect of CAPD solutions on human phagocytes. The authors think that CAPD solutions must be modified to provide a more physiologic pH environment for proper phagocyte function.

Actins↗
PubMedDate not supplied

Use of gas permeable contact lenses following trabeculectomy.

Some patients require a contact lens after glaucoma filtering surgery. The visual rehabilitation of such eyes can be challenging. We report on a total of eight eyes (seven patients) fit with lenticular-design, rigid gas permeable (RGP) contact lenses after the successful establishment of a filtering bleb. In follow-up (mean: 40 months), all patients have successfully worn lenses without adverse effects. We believe that, with careful fitting, close monitoring, and appropriate patient selection, the presence of a filtering bleb need not be a contraindication to contact lens wear, particularly when a smooth-edged, rigid gas permeable (RGP) daily wear lens is used.

Adult↗
PubMedDate not supplied

[Right pulmonary artery-left atrium communication : a rare cause of refractory hypoxia in newborn infants].

Two cases of right pulmonary artery to left atrium in the newborn are described. Both patients presented with refractory hypoxemia cardiomegaly, hypovascular lung fields. Moreover one patient presented with continuous murmur and localised X-ray shadow. Echocardiographic left atrium enlargement suggested diagnosis and angiography confirmed it. Spontaneous improvement in one child is due to a decrease in pulmonary vascular resistance and has allowed the patient to attain an age and a weight in which surgical ligation has been performed more safely.

Abnormalities, Multiple↗