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Biomedical subjects

M Ando

Publications and source records attributed to M Ando.

At least 919 records · Page 51Linked to original sources
PubMed1988

Experimental hypersensitivity pneumonitis in rabbits induced by Trichosporon cutaneum: role of local cellular and humoral immune responses.

We evaluated the immunopathologic properties of Trichosporon cutaneum, an important etiologic agent of summer-type hypersensitivity pneumonitis in Japan. Rabbits were sensitized and challenged by either a culture-filtrate antigen or a particulate antigen prepared from a TIMM 1318 strain of the fungus. Group 1 animals received repetitive intratracheal injections of a particulate T. cutaneum antigen without use of any adjuvant. Group 2 animals were sensitized by foot-pad injections of particulate antigen followed by an intratracheal challenge with the same antigen. Group 3 animals were sensitized by a culture-filtrate antigen emulsified in Freund's incomplete adjuvant followed by a challenge with the intratracheal injection of the same antigen. In group 1, we observed interstitial pneumonitis with prominent granulomatous reactions which resembled those in human hypersensitivity pneumonitis. Group 2 animals also showed granulomatous inflammation in a some minor extent, but they were accompanied with excessive infiltration of neutrophils. In contrast, group 3 showed mild alveolitis in a cell population consisting predominantly of macrophages. Lymphocytes from mediastinal nodes responded to the antigen in groups 1 and 2 but not in group 3. Interestingly, the specific IgA antibody activity in the bronchoalveolar lavage fluids (BALF) of group 1 was significantly higher than those of group 2, despite the IgG antibody activity was the same. The ratio of IgA/IgG antibody activity in individual BALF sample in these two groups correlated inversely with the grade of neutrophil infiltration. Thus, the presence of IgA antibody in the respiratory tract affects the pathological outcome.(ABSTRACT TRUNCATED AT 250 WORDS)

Alveolitis, Extrinsic Allergic↗
PubMed1988

R-wave voltage in the right precordial leads in anthracycline cardiomyopathy: a clinical study.

It was observed that the QS pattern in the right precordial leads of electrocardiograms of patients treated with anthracycline antibiotics resulted in congestive heart failure. In a review of the literature dealing with patients with anthracycline cardiomyopathy, terminal electrocardiograms, when available, showed the QS pattern in the right precordial leads in all patients. The relationship between a decreased R-wave voltage in lead V1 and an increase of the anthracycline dose was evaluated by a clinical study of ten patients. Analysis was subdivided into two categories (group A and B). In group A, there was a dose-response relationship. Electrocardiograms in group B, on the other hand, did not show low R-waves. The present study suggests that increased injury to myocardial cells in the regions of the anterior septum and anterior left ventricular wall might be important in the pathogenesis of anthracycline cardiomyopathy. Patients in group B seemed to be able to tolerate chronic anthracycline cardiac toxicity.

Adolescent↗
PubMed1988

[A case of early gastric cancer forming a large abdominal tumor from a lymph node metastasis].

A 37-year-old male was admitted to our hospital because of an abdominal tumor, about the size of a fist, located anterior to the pancreas head. This tumor oppressed the antrum of the stomach to the left, and an operation was performed, the tumor being diagnosed as a leiomyosarcoma of the stomach, determined from image findings. After histopathological examination, however the tumor proved to be an adenocarcinoma. An early gastric cancer was found at the anterior wall of the antrum during the operation, and the tumor was thought to be a metastatic lymph node. Lymph node metastasis also was observed at the root of the mesentery.

Adenocarcinoma↗
PubMed1988

[Non-clostridial gas-producing brain abscess in a brain death patient--report of a case].

A 46 year old female was admitted to our emergency room because of cardiopulmonary arrest by hanging. After ten minutes cardiopulmonary resuscitation, she was resuscitated but her consciousness did'nt recover. CT-scans on the day of admission showed no abnormalities but on the second hospital day it showed symmetrical low density areas within the basal ganglia, the thalamus, the hippocampus, and the occipital lobe. There were marked enhanced effect in cerebral sulci due to cytotoxic edema of hypoxic encephalopathy. She was comatose for a week, her pupils were dilated, light reflex and other brain stem reflex were absent. CT-scans on the sixth hospital day showed marked brain swelling with disappearance of the ventricular systems (so called brain tamponade). Brain death was confirmed on the basis of Japanese Criteria on the seventh and tenth day of admission. She had been suffering from pneumonia and urinary tract infection with an elevation of temperature since the fourth hospital day. We detected Enterobacter Cloacae (E. Cloacae), Klebsiella Oxytoca from the cultures of sputum and urine. On the tenth hospital day her temperature was running up to 39.4 degrees C and blood count revealed a peripheral blood leucocytosis of 40,300/mm3 with a shift to the left. E. Cloacae was also detected from the cultures of blood. Skull roentgenogram showed multiple gas collections in the ventricular systems. CT-scans on the fourteenth hospital day showed multiple gas-containing brain abscess. The etiology of this infection was considered due to septicemia of E. Cloacae. She died from acute renal failure on the fifteenth hospital day. Consent for autopsy was not accepted.(ABSTRACT TRUNCATED AT 250 WORDS)

Brain Abscess↗
PubMed1988

[A case of olfactory hallucination with adult enuresis].

A 22-year-old man was referred to us complaining of enuresis. The excretory urogram and cystogram were almost normal. Spina bifida was not present. The results of the urodynamic examination including uroflowmetry, cystometry, urethral pressure profile, electromyogram and sensory threshold of external urethral sphincter were within normal limits. Amitriptyline hydrochloride and flavoxate hydrochloride showed no effects. Because we found a positive spike wave on the electroencephalogram, the patient was referred to a psychopathologist, and was diagnosed as being in the early stage of schizophrenia. He complained of enuresis as his olfactive hallucination of urine. Psychopathological treatment for 1 year and 2 months resulted in disappearance of his complaining of enuresis.

Adult↗
PubMed1988

R-wave voltage in the right precordial leads in anthracycline cardiomyopathy: experimental animal model.

Electrocardiographic abnormality, similar to that observed in cancer patients during chemotherapy with the anthracycline antibiotics daunomycin and adriamycin, was produced in rabbits. They were chronically treated with adriamycin, and before each administration of the drug an electrocardiogram was recorded. They were subdivided into two categories with respect to the R-wave voltage in lead V1, on the same basis as our previous investigation in humans. In group A, there was a dose-response relationship, and they ultimately showed a QS pattern in the right precordial leads with or without ST-T changes in the left precordial leads. In group B, the electrocardiograms did not show a low R-wave in lead V1, but ST-T changes in the left precordial leads. The similarity of these electrocardiographic changes to those produced by myocardial infarction that we observed in humans suggests that more severe injury to the myocardium at the anterior septum and anterior left ventricular wall may be important in the pathogenesis of anthracycline cardiomyopathy.

Animals↗
PubMed1987

Determination of acid phosphatase in biological fluids using a new substrate, 2,6-dichloro-4-nitrophenyl phosphate.

A new substrate, 2,6-dichloro-4-nitrophenyl phosphate (DCNP-P), is used for the determination of acid phosphatase (EC 3.1.3.2) in serum and urine. It was hydrolyzed by acid phosphatase to 2,6-dichloro-4-nitrophenol (DCNP) and phosphoric acid. At a pH of 4.5-6.0, the absorption of DCNP liberated by acid phosphatase was much higher than that of p-nitrophenol, which is commonly used as an aglycone in the acid phosphatase assay. By using DCNP-P as a substrate for acid phosphatase activity, determinations can be made without the colour reaction which requires the addition of an alkaline solution, and can be determined by the rate assay that does not require measurement of sample blanks in serum or urine. This method using DCNP-P is highly sensitive and is the most suitable for the rate assay of acid phosphatase activity in biological fluids.

Acid Phosphatase↗
PubMed1987

Influence of cadmium on the metabolism of vitamin D3 in rats.

In order to obtain further information on the effects of cadmium (Cd) on the mechanism of activation of vitamin D3 in the kidney, the serum concentrations of 1,25-dihydroxycholecalciferol [1,25(OH)2D3] and 24,25-dihydroxycholecalciferol [24,25(OH)2D3] were determined by radioimmunoassay techniques. The serum concentration of 1,25(OH)2D3 in Cd-exposed rats was always higher than that in control rats. The concentrations of serum 1,25(OH)2D3 in parathyroidectomized rats (PTX), both in the control and in the Cd-exposed groups, were markedly lower than those in non-PTX rats. On the other hand, the concentration of serum 24,25(OH)2D3 in Cd-exposed rats was less than that in control rats. In other words, the pathway from secretion of parathyroid hormone (PTH) to synthesis of 1,25(OH)2D3 or 24,25(OH)2D3 was normal, but the pathway from stimulation of PTH to secretion of PTH was abnormal. These results suggest that rats exposed to Cd for 90 days were in a state of either deficiency of 1,25(OH)2D3 or excess secretion of parathyroid hormone. Although hypophosphatemia occurred in the Cd-exposed rats, the 1,25(OH)2D3 serum level in rats was not increased by PTX. On the basis of these results, it is suggested that hypophosphatemia occurring after the exposure of rats to Cd is a secondary hypophosphatemia.

24,25-Dihydroxyvitamin D 3↗
PubMed1987

Absent aortic valve with normally related great arteries.

A neonate with absent aortic valve associated with membranous mitral atresia and normally related great arteries is reported. The patient was a 3360-g boy and was transferred to our hospital 4 h after birth because of severe cyanosis and respiratory distress. Absent aortic valve associated with mitral atresia was diagnosed on echocardiography. The patient died 4 days later with congestive heart failure. Postmortem examination revealed the aortic valve and sinus of Valsalva to be totally absent. The mitral valve showed membranous atresia. Severe endocardial fibroelastosis of the left ventricle, which was mildly dilated, and bizarre trabeculation were seen. This is the first case report of an absent aortic valve with normally related great arteries.

Aortic Valve↗
PubMed1987

An infant with dilated cardiomyopathy confirmed as myocarditis by endomyocardial biopsy.

A case of an 11-month-old infant who was incidentally diagnosed as having myocarditis by right and left ventricular endomyocardial biopsy is reported. This patient was admitted because of heart failure. Chest X-ray showed cardiomegaly and an electrocardiogram revealed decreased voltage with right atrial and right ventricular overloading. A dilated left ventricle with poor ejection fraction was shown in an echocardiogram. Dilated cardiomyopathy was the initial diagnosis since there was no episode of preceding viral infection or evidence of an inflammatory process in routine laboratory investigations. However, the biopsy findings, including massive infiltration of inflammatory cells and myocyte degeneration, indicated myocarditis. To our knowledge, this is the youngest patient in whom myocarditis has been confirmed by endomyocardial biopsy.

Biopsy↗
PubMed1987

Effect of cadmium on vitamin D-non-stimulated intestinal calcium absorption in rats.

The present report demonstrates the effect of Cd on intestinal Ca transport and Ca-binding protein in rats adapted to vitamin D repletion and high Ca. There were significant decreases in in vitro intestinal Ca transport in animals exposed to Cd for 2 or 3 months as compared with controls. Ca transport from everted gut sacs of normal rats showed a dose-dependent decrease. 45Ca-binding activity to intestinal mucosa from Cd-exposed rats showed a lower level of decrease than that on control rat mucosa. The typical peak of Cd-binding protein obtained from Sephadex G-100 gel filtration of 109Cd added to mucosal supernatant agreed with results of gel filtration obtained after 45Ca had been added to the same supernatant. Although there were 2 proteins binding Cd or Ca in the intestinal mucosa, with the same molecular weight of 10,000, these proteins were shown to be different, since Cd did not bind with the Ca protein. It was concluded that inhibition of vitamin-D-non-stimulated Ca transport was not caused by Cd bound to Ca-binding protein, but by some other factor.

Animals↗