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M A Overbeeke

Publications and source records attributed to M A Overbeeke.

At least 55 records · Page 3Linked to original sources
PubMed1990

Murine monoclonal antibodies against a unique determinant of erythrocytes, related to Rh and U antigens: expression on normal and malignant erythrocyte precursors and Rhnull red cells.

Three murine monoclonal antibodies (Mabs) MB-2D10, LA-18.18 and LA-23.40 were prepared. They reacted with red cells of all common and most rare blood-group phenotypes, with the exception of those of the RhnullU negative and RhmodU negative phenotypes. So far, only a single example of an alloantibody (Duclos or anti-Rh38) of a similar specificity has been found. Serological studies indicated that the Mabs were probably not directed against an antigenic determinant of Rh polypeptides, the LWab glycoprotein or glycophorin B, all structures absent from or aberrantly expressed on Rhnull red cells. The antigen was found to be erythrocyte-specific, and was also present on pro-erythroblasts, erythroblasts and malignant erythroblastoid cells but not on erythroid progenitors in the bone marrow. The Mabs were found to block each other in an immune rosette method and are thus probably directed against the same epitope or against neighbouring epitopes on the same structure. In immunochemical studies, MB-2D10 precipitated the 30-32 kDa Rh polypeptides from red cell membranes and a protein or proteins which formed diffuse and overlapping bands in SDS-polyacrylamide gel electrophoresis, with Mrs of 40-200 kDa (probably the Rh-related glycoproteins). Under certain experimental conditions glycophorin B appeared to be coprecipitated. The 2D10 structure, detected by the Mabs, seems to be part of a complex of proteins and/or glycoproteins, which includes Rh polypeptides, the LWab glycoprotein and glycoproteins recognized by various Mabs with Rh-related specificities. In the red cell membrane, the complex may be associated with glycophorin B.

Animals↗
PubMed1990

A new, low-incidence red cell antigen (HOFM), associated with depressed C antigen.

A case of mild hemolytic disease of the newborn is presented which was caused by an antibody to a hitherto unknown antigen of low incidence. This antigen, now designated as HOFM (ISBT number 700050) was detected in 6 relatives, and in all of them, it was associated with an unusually weakened expression of C antigen. The serological data indicate that HOFM may be part of the Rh system, but the genetic data, although supportive of this interpretation, are inconclusive.

Adult↗
PubMed1990

Evaluation of the polyethylene glycol antiglobulin test for detection of red blood cell antibodies.

The polyethylene glycol (PEG) antiglobulin test (AGT) was evaluated by testing antibodies in the sera of 362 different patients and by screening of 4,685 random patient sera with both the PEG AGT and the albumin AGT. Agglutination in the PEG method was stronger in 254 (70.2%), identical in 99 (27.3%) and weaker in 9 (2.5%) of the 363 sera. Mean end-point titers of the antibodies measured with the PEG method (n = 137) were 2-3 dilutions higher than with the albumin method. In sera with anti-K and anti-Fyb antibodies the mean titer in the PEG method was only one dilution higher. Antibodies within the rhesus system were also tested with a two-stage enzyme test. Agglutination in the PEG method was stronger in 93 (50.3%), identical in 64 (34.6%) and weaker in 28 (15.1%) of the 185 sera. Mean antibody titers (n = 76) in the PEG method were 1-3 dilution steps higher for the various specificities than in the enzyme test. In 92 (2.0%) of the 4,685 sera screened in the PEG method, specific antibodies were detected; with the albumin method in 74 (1.6%) sera. The antibodies only detected with the PEG method had the specificities anti-D,-C,-Cw,-c,E,-K,-Jka and -M. Not detected by the PEG method were anti-Lea antibodies in one of the sera. It is concluded that the PEG AGT is a sensitive technique for the detection and identification of red cell antibodies and that it is superior to the albumin AGT.

Coombs Test↗
PubMed1989

[Prenatal fetal blood group determination using chorionic villi biopsy].

The presence or absence of Rhesus D, c and Kell antigens on foetal red blood cells was determined in the first trimester of pregnancy on erythrocytes obtained by chorionic villi sampling. Pregnancies in 15 severely sensitized women (9 Rh D, 5 Kell and I Rh c) with a poor obstetric history and a partner heterozygous for the offending antigen were examined. A conclusive diagnosis could be made in 13 of the 15 cases studied.

Blood Group Antigens↗
PubMed1989

Testing efficacy of anti-D sera by a panel of donor red cells with weak reacting D antigen and with partial D antigens.

In 1987 the definition of an Rh-negative donor in the Netherlands was changed from C-, E- as well as Du-negative to D-negative only. The use of 2 different strong anti-D sera without an antiglobulin phase (Du test) was considered sufficient to reveal the clinically important D antigen. In applying this policy, we identified 32 donors in 13,500 consecutive blood donations whose indirect antiglobulin test (IAT) (Du)-positive red cells gave negative reactions with at least 2 out of 11 anti-D sera and whose cells might therefore be typed as Rh(-D-)-negative in routine investigations. IgG anti-D used by a one-stage bromelain technique and anti-D with modified IgG appeared to be relatively insensitive in detecting Du in this study. Polyclonal anti-D in an enhancement medium and monoclonal anti-D scored better, although differences exist between the products of various manufacturers. It is suggested that if IAT (Du) testing is omitted, only anti-D sera with a high index of detectability of cells expressing weak D antigens should be accepted.

Antibodies, Monoclonal↗
PubMed1988

What is the best predictor of the severity of ABO-haemolytic disease of the newborn?

In 80 newborn infants ABO-incompatible with their mothers, the lysis-inducing effect of the maternal IgG anti-A or anti-B antibodies in an antibody-dependent cell-mediated cytotoxicity (ADCC) assay and the antigen density of A or B antigens on the red cells of the children were measured. On the basis of the results, the children were divided into two groups--24 children in whom increased haemolysis was to be expected, and 56 children in whom it was not. Signs of haemolysis and serological features of ABO haemolytic disease of the newborn (ABO-HDN) were compared in these two groups and a control group of 120 ABO-compatible infants. The effect of the maternal antibodies in the ADCC assay, the titres of maternal IgG anti-A or anti-B antibodies, the results of the direct antiglobulin test on the red cells in the cord blood, and the titre of IgG anti-A or anti-B antibodies in the serum of the infants were compared for their ability to predict the severity of ABO-HDN. This was also done for the combination of the ADCC assay results plus the A or B antigen density and the direct antiglobulin test plus the titre of maternal IgG anti-A or anti-B antibodies. The ADCC assay with maternal serum was the most sensitive assay to predict ABO-HDN, and the combination of the ADCC assay with A or B antigen density determination the most specific test.

ABO Blood-Group System↗
PubMed1988

Maternal antibodies against fetal blood group antigens A or B: lytic activity of IgG subclasses in monocyte-driven cytotoxicity and correlation with ABO haemolytic disease of the newborn.

IgG antibodies against blood group antigens A or B (anti-A/B) are able to sensitize erythrocytes for destruction in an antibody-dependent cell-mediated (ADCC) assay with monocytes as effector cells. The activity of maternal IgG anti-A/B in this test was compared with clinical signs of haemolytic disease of the newborn (HDN). When the ADCC was negative (less than 10% of the sensitized cells lysed), signs of increased red-cell destruction in the children were never observed. In three cases with a strongly positive ADCC (greater than 45% lysis), the children were severely affected and needed more than one exchange transfusion. In the cases with greater than 10% but less than 45% lysis in the ADCC, there was no clear correlation between the result of the ADCC and the degree of lysis in the newborn infants. In these cases, the degree of lysis of the red cells of the infant was shown to be strongly influenced by the number of A/B antigens per red cell. There was a direct correlation between the degree of lysis in the ADCC and the titre of IgG3 anti-A/B in the sera. There was comparable activity of maternal IgG anti-A/B in the ADCC test in the 32nd week of pregnancy and at the moment of delivery.

ABO Blood-Group System↗
PubMed1988

Complement is not activated in ABO-haemolytic disease of the newborn.

We studied the lysis in vitro of group A red cells by IgG anti-A. IgG anti-A, which strongly lysed A red cells from adults, did not lyse A red cells from cord blood, if fresh cord serum from a child with blood group AB was used as a source of complement. In cases of haemolytic disease of the newborn due to A-O or B-O antagonism with a positive direct antiglobulin test with anti-IgG serum, the red cells did not react with anti-complement sera. Apparently, complement is also not activated in vivo in case of A-O haemolytic disease of the newborn.

ABO Blood-Group System↗
PubMed1987

Fetal blood group determination in first-trimester pregnancy for the management of severe immunization.

The presence or absence of Rho (D) and Kell antigens on fetal red blood cells was determined in the first trimester of pregnancy on erythrocytes obtained by chorionic villi sampling with the use of mixed agglutination and solid phase microimmunofluorescence techniques. Pregnancies in one Kell-sensitized woman and seven severely RH-sensitized women with a poor obstetric history and a partner heterozygous for the offending antigen were examined. A conclusive diagnosis could be made in seven of the eight cases studied.

Blood Grouping and Crossmatching↗
PubMed1987

Sensitive methods for determining subclasses of IgG anti-A and anti-B in sera of blood-group-O women with a blood-group-A or -B child.

The determination of the subclasses of IgG antibodies against blood groups A and B is important in order to improve our understanding and predict haemolytic disease of the newborn due to IgG anti-A or -B. We describe two techniques that circumvent the problem of the agglutination of A and B red cells by the corresponding IgG antibodies in saline: an antiglobulin consumption test and a modified solid-phase micro-immunofluorescence test. The results of the two techniques are compared with the results obtained in the indirect antiglobulin test beyond the saline agglutination titre in a microplate technique. The solid-phase micro-immunofluorescence test was the most sensitive for the determination of the subclasses of IgG anti-A and -B. Usually sera contained IgG2 anti-A, B in a higher titre than antibodies of other subclasses.

ABO Blood-Group System↗
PubMed1986

ABO and Rhesus phenotyping of fetal erythrocytes in the first trimester of pregnancy.

Determination of fetal red blood cell antigens in early pregnancy can be important in cases with a history of severe haemolytic disease of the newborn. From chorionic villus biopsies (CVB) between the 8th and 12th week of gestation a small number of fetal red blood cells was obtained, inevitably highly contaminated with maternal blood cells. Two techniques were used to demonstrate the minor (fetal) cell population with a blood group antigen differing from the major (maternal) cell population: a solid-phase microfluorescence technique (introduced in this paper) which was compared with the mixed agglutination technique. In series of artificial mixtures of erythrocytes it was shown that with the microfluorescence technique the ABO and Rhesus phenotypes of minor cell populations could be determined at a ratio of 1 in 4000 erythrocytes of the major population, making this technique 4 times as sensitive as the mixed agglutination technique. We further investigated the reliability of the microfluorescence technique to demonstrate antagonistic fetal blood groups in the first trimester of pregnancy. Of 18 women undergoing CVB prior to therapeutic abortion, blood group antagonism (ABO and Rhesus systems) was demonstrate in all 11 cases in which it was present. Therefore, it seems that CVB can be reliably used for the prenatal diagnosis of (recurrent) blood group antagonism.

ABO Blood-Group System↗
PubMed1984

Red-cell auto-antibodies mimicking anti-Fyb specificity.

An antibody is described, produced by an Fy(a+b-) woman that at first reacted with her own cells, preferentially with Fy(b+) cells but not with Fy(a-b-) cells. Later, pure anti-Fyb specificity occurred. Elution and absorption studies revealed that the antibody recognized a determinant absent from Fy(a-b-) cells but present on Fy(a+b-) and Fy(a-b+) cells, much more strongly, however, on the latter, and that it was an autoantibody.

Anemia, Hemolytic, Autoimmune↗
PubMed1981

A negative direct antiglobulin test with strong IgG red cell autoantibodies present in the serum of a patient with autoimmune haemolytic anaemia.

The case is presented of a boy with an autoimmune haemolytic anaemia of 10 years duration. He had a positive direct antiglobulin test with IgG and complement detected on the red cells and with IgG autoantibodies in the serum. During a recent episode of severe haemolysis, the Hb level fell to 3.8 g/dl and the direct antiglobulin test became negative although his autoantibodies still reacted with all the red cells in a panel. The serum reacted more strongly with C- and e-positive cells. The Rh phenotype of the patient was CcDee as it had always been. Possible explanations of the unexpected findings are discussed.

Adolescent↗