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Biomedical subjects

M A Memon

Publications and source records attributed to M A Memon.

At least 73 records · Page 4Linked to original sources
PubMed1994

Conception by use of postmortem epididymal semen extraction in a dog.

A 9-year-old sexually intact male Boxer was euthanatized because of progressive, generalized seizures that were refractory to medical treatment. To try to preserve the breeding line, postmortem epididymal semen extraction was attempted. The testes were excised after the dog was euthanatized, and semen was extracted, frozen, and stored in liquid nitrogen. Approximately 3.5 months later, a suitable recipient was identified, and surgical intrauterine insemination was performed. Fifty-seven days after insemination, a viable pup was born.

Animals↗
PubMed1994

A change of Plasmodium species infecting children in Karachi over the last decade.

Febrile children of both sexes, aged between 0-15 years coming to the Civil Hospital over the last 12 years had their blood tested for malarial parasite. Five hundred and twenty-six slides positive for Plasmodium were analysed for species and stage identification. Of the total 59.3% children with malaria were between 5-15 years of age. Applying the test of proportion, Plasmodium vivax was the predominant species (P < 0.01) between 1981-1985 and plasmodium falciparum between 1986-1990. This trend persisted upto 1992. As malaria due to Plasmodium falciparum is more severe with multiple complications accurate and easier methods of its diagnosis are needed at primary health care level.

Adolescent↗
PubMed1993

Chronic vaginal prolapse during pregnancy in a bitch.

A 4-year-old bitch was referred with chronic vaginal prolapse. It was first noticed during estrus, about 58 days prior to referral. Artificial insemination (AI) was performed twice after reducing the prolapse manually. Abdominal radiography (53 to 55 days after AI) was performed by the attending veterinarian and the bitch was determined to be not pregnant. Serum progesterone and estradiol concentrations were 1.38 ng/ml and 1.79 pg/ml, respectively. With the bitch under general anesthesia, the vaginal prolapse was partially reduced and a hysteropexy was performed. Six days after surgery, the bitch delivered a live male pup. It is recommended that bitches so affected should not be bred, and that those not required for breeding should be ovariectomized.

Animals↗
PubMed1993

The relationship of semen quality to pregnancy rate and litter size following artificial insemination in the bitch.

Artificial insemination of 31 bitches with fresh, undiluted, semen resulted in 22 31 conceptions (71%) and the birth of 124 puppies. Inseminations using at least 220 x 10(6) spermatozoa of normal morphology resulted in a mean pregnancy rate of 81.5% (22 27 ). The pregnancy rates were significantly influenced by the total number of progressively motile or morphologically normal sperm cells per ejaculate (P < 0.05). The pregnancy rate was not influenced by the percentage of progressively motile or morphologically normal sperm cells per ejaculate (P > 0.1). Litter size was not influenced by sperm motility or morphology (P > 0.1).

Journal Article↗
PubMed1993

Simian immunodeficiency virus infection via amniotic fluid: a model to study fetal immunopathogenesis and prophylaxis.

The rising prevalence of infection with the human immunodeficiency virus type 1 (HIV-1) in young women will increase the number of infected children worldwide. Because HIV-1 seems to be transmitted mostly intrapartum, fetal infection probably occurs mainly via skin or mucous membrane exposure. A model for this route of fetal infection has been established in primates. After injecting the simian immunodeficiency virus (SIV) into amniotic fluid during late gestation, six of seven rhesus monkeys were born infected. All infected neonates were viable and showed signs of disease, such as low birth weights, lymphadenopathy, and rashes. Cytotoxic T-cell responses to SIV were absent in neonates, but present in mothers. The high fetal infection rate allows studies of lentiviral immunopathogenesis during ontogeny and the development of strategies to prevent maternal HIV-1 transmission.

Amniotic Fluid↗
PubMed1993

Laparoscopic closure of acutely perforated duodenal ulcer--an early experience.

Laparoscopic closure of an acutely perforated duodenal ulcer is an alternative procedure to open surgery. With proper training and experience this procedure might overtake laparotomy and simple closure thereby reducing the post operative morbidity in terms of reduced wound pain, short hospital stay, likely reduced wound sepsis and hernia occurrence and post operative chest complications. This article describes four patients with acute perforation of duodenal ulcer who were submitted to an emergency laparoscopic repair.

Aged↗
PubMed1992

Use of human chorionic gonadotropin stimulation test to detect a retained testis in a cat.

A 4-year-old male cat was referred because of aggressive behavior and spraying urine. When the cat was 6 months old, only 1 testis was found in the scrotum. When the cat was 1 year old, the scrotal right testis was surgically removed and the left testis was not found, either within the scrotum or within the abdomen. The cat developed male behavior and another laparotomy was performed 1 year later, at which time the left testis could not be located. The cat continued to show male behavior. On referral, the penis was well developed and had spines. Human chorionic gonadotropin (HCG), 500 IU, was administered IV. At baseline, 30, and 120 minutes after HCG administration, serum testosterone activity (ng/ml) was 0.68, 5.0, and 10.5, respectively. Laparotomy was performed with the cat under general anesthesia. The left testis was found in the facial plane lateral to the symphysis pubis. Six weeks after the surgery, the HCG stimulation test was repeated and testosterone was not detected in any serum sample. The most practical solution to locate the undescended testis would have been to follow the intact ductus deferens to the testis adjacent to the pubic symphysis.

Animals↗
PubMed1991

Transgenic production of a variant of human tissue-type plasminogen activator in goat milk: generation of transgenic goats and analysis of expression.

We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA--longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3 micrograms/ml.

Animals↗
PubMed1990

Culture of 5-day horse embryos in microdroplets for 10 to 20 days.

Embryos were recovered from the uteri of mares 5 d after ovulation. Six embryos, all morulae, were placed singly in 200-ul droplets of Ham's F-12 with 10% fetal calf serum and cultured at 37 degrees C in a 5% CO(2) atmosphere. The embryos expanded to form blastocysts by the third day of culture. The blastocysts hatched from their zona pellucida, rather than the zona thinning and flaking off, as occurs in vivo. Hatching from the zona pellucida began on the third day of culture and was complete in five of six embryos by the sixth day. The embryonic capsule, normally present in equine embryos after Day 6, was not seen in the cultured embryos. The blastocysts continued to expand until 15 to 17 d of age (10 to 12 d in culture), reaching an average diameter (+/- SD) of 2052 +/- 290 um, after which time they either collapsed or contracted. These results demonstrate that equine embryos can be maintained in long-term culture in vitro, exhibiting continued growth and expansion in the absence of the embryonic capsule.

Journal Article↗
PubMed1989

Bacteremic Hemophilus influenzae pneumonia in the adult.

Bacteremic Hemophilus influenzae pneumonia is an uncommon infection in the adult. It usually affects chronically ill patients, especially those with chronic obstructive lung disease, although healthy individuals can also be affected. The condition carries a significant mortality, reaching 57% in one series. We describe ten adult patients with bacteremic H influenzae pneumonia with some unique features: no deaths occurred, half of the involved patients were previously fit individuals, and a beta-lactamase producing strain was isolated in five out of ten patients. An interesting finding was the isolation of a beta-lactamase negative H influenza from the sputum of two patients whose blood cultures were positive for a beta-lactamase positive H influenza. The overall incidence of beta-lactamase production among bacteremic isolates was 50%--a finding with a great deal of impact on the treatment of this disease.

Adult↗
PubMed1988

Preputial injuries in beef bulls: 172 cases (1980-1985).

One hundred seventy-two bulls with preputial injuries were treated from 1980 through 1985. Most of the injuries developed during the breeding season (April through August), with a peak during June. The number (111) of bulls represented by 3 beef breeds (Brangus, Brahman, Beefmaster) was greater than the number (61) of the other 9 breeds included in the survey. Preputial prolapse (PP) and posthitis without obvious laceration was diagnosed in 82 of 172 bulls. Preputial laceration, abscessation, stricture with phimosis, and PP with penile hematoma were diagnosed in 46, 22, 15, and 9 bulls, respectively. These abnormalities were treated medically and/or surgically. Questionnaires (n = 145) were mailed, and 83 (57.4%) replies were received. Owners (80/83) of affected bulls responded to questions regarding the future use of their bulls for breeding. Of the bulls with PP and posthitis (n = 34), laceration (n = 17), abscessation (n = 15), stricture with phimosis (n = 8) and PP with penile hematoma (n = 6) and treated medically and/or surgically, 30, 11, 7, 7, and 4 bulls, respectively, (74% overall) were returned to breeding use successfully.

Animals↗