Autologous fibrin for blepharoplasty incisions.
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Adult↗
Biomedical subjects
M A Mandel
Publications and source records attributed to M A Mandel.
Biotinylated nucleic acid hybridization probes for potato virus detection.
cDNA libraries, representative of potato viruses X (PVXc strain) and Y (PVY degrees strain) genomes were obtained. A PVX cDNA cloned fragment was sequenced and biotinylated to be used as hybridization probe for the detection of purified virus or nucleic acid extracts of infected plants. Dot hybridization assay was sensitive to detect 4 ng of viral particles, corresponding to about 200 pg of viral RNA. The level of detection in infected plant extracts was as effective as that obtained with the ELISA. The presence of biotinylated PVY cDNA in the hybridization mixture did not affect sensitivity of the PVX detection assay, suggesting that a single diagnostic assay for several potato viruses and virus-related pathogens could be developed.
Amino Acid Sequence↗
Autotransfusion in elective plastic surgical operations.
One-hundred and eight patients undergoing elective major plastic surgical procedures, primarily reduction mammaplasties, have been autotransfused. Units of blood were harvested 1 and/or 2 weeks before the operation. At the time of the surgery, the patient's hematocrit and hemoglobin had almost returned to predonation level and active reticulocytosis was occurring. No patients in this group had any problems from phlebotomy or autotransfusion. These autotransfused patients felt better, were less anxious about acquiring a transmitted disease, and returned to work or school activities earlier than those in the control groups. Autotransfusions are safe and effective and this pilot study indicates that they should be considered for patients undergoing major elective procedures.
Abdominal Muscles↗
Fibrodysplasia ossificans progressiva: a distinctive bone-forming lesion of the soft tissue.
A 3-month-old white female presented with a bone-forming lesion of the soft tissue of the left cheek. Skeletal survey revealed numerous associated radiographic abnormalities, predominantly involving the phalanges. Mineral metabolism and endocrine function were within normal limits. Multiple operations were performed over a 17-year period for palliation of sequelae arising from inability to open the mouth due to ankylosis of the temporomandibular joint by the progressively ossifying lesion. Histologically, the pathologic material had features resembling those of periosteal grafts, with all stages of membranous bone formation and a tendency for more mature lesions later in the course. .The clinico-pathologic features are those of fibrodysplasia ossificans progressiva (FOP). The differential diagnosis of this rare condition from other bone-forming lesions of the soft tissue such as myositis ossificans, extra-skeletal osteosarcoma and osseous metaplasia is discussed.
Bone Neoplasms↗
The management of lower extremity degloving injuries.
Successful treatment of massive degloving lower extremity requires a flexible approach with adherence to basic surgical principles. There should be aggressive therapy with extensive debridement guided by direct observation and fluorescein vital dye staining. Wound closure, either primary or delayed, should be carried out using autogenous tissues when local and systemic tissue conditions permit.
Adolescent↗
Squamous cell carcinoma arising in a linear epidermal nevus.
An isolated, pruritic lesion developed in the right breast of a 17-year-old girl during a period of four months. The lesion was well circumscribed, erythematous, indurated, and markedly hyperkeratotic in the center. The histologic diagnosis was well-differentiated squamous cell carcinoma arising in a linear epidermal nevus. The development of squamous cell carcinoma in a linear epidermal nevus is a rare event, previously reported only in long-standing, extensive lesions of middle-aged to elderly patients. This case seems to be unique in regard to the patient's young age, the short duration of the nevus, and the lack of identifiable predisposing factors.
Adolescent↗
Squamous cell carcinoma arising in pemphigus vulgaris during immunosuppressive therapy.
A rapidly growing squamous cell carcinoma developed in an area of active pemphigus vulgaris on the cheek of a 45-year-old woman during treatment with prednisone and cyclophosphamide. This is the first reported case of this phenomenon. The roles of solar and drug carcinogenesis, autoimmunity, and immunosuppression in the pathogenesis of this tumor are discussed.
Adult↗
"Hiawatha" scrotal reconstruction.
Total reconstruction of the scrotal sac in a young adult male is described. A normal-appearing scrotum and functional testicles resulted from the two-stage procedure, in which the testicles were first implanted in the thighs, where they developed firm pseudocapsules around themselves, and these fibrous elements were then turned on themselves and sutured together in the second stage to provide a smooth vascular bed for split skin graft coverage. Three-year follow-up reveals normal sperm count.
Adolescent↗
Osteoradionecrosis of the thoracic wall and its surgical management.
Four patients with disabling radiation defects of the chest involving skeletal and soft tissues were successfully treated with a one stage breast flap. Closure of the defect resulted after an uncomplicated and safe operation. Safety and reduced morbidity are hallmarks of the breast flap repair for this difficult problem. Although alternative methods can be used, the preparation of staged flaps and postoperative problems in major myocutaneous flaps increase the risk of such therapy and the hospital stay for these elderly, debilitated patients. The low morbidity of this one stage flap with a minimal hospital stay make this treatment a very viable and appealing alternative for patients whose long-term prognosis is exceedingly poor.
Aged↗
Immune competence and diabetes mellitus. II. Experimental mouse studies.
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Animals↗
Cornifying Darier's disease.
Five patients with cornifying Darier's disease were treated successfully by partial-thickness resection of the skin and dermabrasion down through the papillary dermis into the reticular dermis. Healing was quick and the complication rate was low. Although some lesions recurred, all of these 5 patients have been able to return to full work and are now employed. The histology and genetics of the disease are discussed.
Adult↗
Seizures abolished by excision of a cavernous hemangioma of the scalp and skull.
A large vascular malformation of the supraorbital and scalp areas was present in an adolescent girl who had seizures and headaches. Removal of the malformation included the outer table of the skull. The patient has had no seizures during a two-year follow-up.
Child↗
Lethal irradiation: treatment with immune cell populations.
Lethally irradiated mice have been successfully treated by the inoculation of allogeneic spleen cell populations, without the development of a secondary graft-versus-host (GVH) reaction. This has been accomplished by eliminating those cells responsible for GVH reaction, using a density gradient ultracentrifugation technique. The therapeutic potential for this technique is great. It may well provide a modus operandi to treat patients exposed to otherwise lethal doses of radiation, as well as facilitate therapy of patients with cancer and immune deficiency diseases.
Animals↗
Immune competence and diabetes mellitus: pyogenic human hand infections.
An unexpectedly high incidence of diabetes mellitus was found in 12 patients with pyogenic hand infections. Three patients had overt diabetes, four had latent diabetes, and only five were normal when tested after resolution of the acute inflamatory state. Cellular immunity, assessed in five patients by intradermal injections of common antigens and the topical application of the neoantigen, dinitrochlorobenzene, appeared to be grossly normal in all patients. If changes in immunity were present, it is likely that the skin tests were not sensitive enough to detect these alterations.
Adolescent↗
Impairment of cell-mediated immunity in mutation diabetic mice (db/db).
Mutation diabetes in the mouse occurs in the C57BL/Ks strain. All homozygous animals (db/db) develop obesity, elevated blood sugar levels and increased or normal blood insulin concentration. The defects in cellular immunity in db/db mice and their littermate controls were examined both in vivo and in vitro. Significant suppression of delayed footpad swelling and first and second set skin allograft rejection time were observed. In addition, DNA synthesis in spleen cells after nonspecific mitogen stimulation was markedly inhibited. Diabetic animals with a mean blood sugar of 512 +/- 101 mg/100 ml did not respond to exogenous insulin therapy by lowering their blood sugar levels or reversing the defect in DNA synthesis. Adding insulin to spleen cell culture in vitro had no demonstrable effect on their response to mitogens. Thus, mutation diabetic mice with their known defect in the peripheral utilization of insulin have markedly suppressed cell-mediated immune mechanisms.
Animals↗
Subcutaneous mastectomy with immediate reconstruction of the large breast.
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Breast↗
Radial artery cannulation in 1,000 patients: precautions and complications.
A review of 1,000 radial artery cannulations performed over a 2-year period showed that, with careful selection of patients, by means of the Allen test and Doppler studies, and with the apparatus used, only two serious complications were encountered; embolectomy was necessary in one and arterial reconstruction in another. Twenty-four percent of patients had evidence of diminution of flow, but in none did it persist for more than 2 weeks. Critical factors in reducing complications were a short period of cannulation, use of a Teflon catheter, and a continuous arterial line flush system.
Arm↗