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Biomedical subjects

L Wolf

Publications and source records attributed to L Wolf.

82 records · Page 5Linked to original sources
PubMedDate not supplied

Immunobiology of cellular transplantation.

The goal of cellular transplantation is to allow long-term function of the grafted cells using minimal host immunosuppression. To this end, the major strategies to implant cells and tissues are through: (i) the pretreatment of the graft to reduce tissue immunogenicity; (ii) the application of immunoisolation technologies to prevent host sensitization to implanted cells; and (iii) the induction of immunological tolerance to the donor tissues. Further, a major dilemma facing clinical tissue grafting is the shortage of donor tissue for transplantation. This problem requires the consideration of tissues from other species (xenografts) as a potential source of donor material. In light of these issues, the focus of this discussion is on the T cell-dependent response to allogeneic and xenogeneic transplants and the implications of this reactivity on the field of cellular replacement therapy.

Animals↗
PubMedDate not supplied

[Adrenal adenoma disclosing after delivery].

Pregnancy and Cushing's syndrome are seldom found together (40 cases in the literature), since hyperadrenocorticism is often responsible for anovulation by gonadotropin suppression. We report the case of a 25-year old para II woman whose pregnancy was complicated by diabetes and arterial hypertension at 31 weeks and who received the conventional treatments (special diet, insulin therapy, pindolol). Caesarean section, motivated by premature rupture of the membranes, was performed at 37 weeks, delivering a healthy infant. The diagnosis of hypercortisolism with low ACTH level was made post partum. An adrenal tumour (the most frequent cause of Cushing's syndrome occurring during pregnancy) was removed after pre-operative treatment with ketoconazole, and endocrine functions returned to normal.

Adenoma↗
PubMedDate not supplied

Human osteoblast-like cells contain specific, saturable, high-affinity glucocorticoid, androgen, estrogen, and 1 alpha,25-dihydroxycholecalciferol receptors.

Glucocorticoids, androgens, estrogens and 1 alpha,25-dihydroxycholecalciferol (1 alpha,25-(OH)2D3) exert a variety of effects on bone homeostasis. We have measured the receptors for these hormones in cultured human osteoblast-like cells. Specific binding was found with each of the four steroids tested, namely maximum binding capacities, Bmax, of 2,581 (1,368-4,223), 146 (101-237), 176 (20-552), and 387 (290-620) fmol.mg DNA-1 (mean, range) for [3H]dexamethasone, [3H]5 alpha-dihydrotestosterone, [3H]17 beta-estradiol ([3H]E2), and [3H]1 alpha,25-(OH)2D3, respectively. The corresponding apparent dissociation constants were 13.3 (9.2-22.2), 0.43 (0.23-0.94), 0.45 (0.17-0.88), and 0.020 (0.008-0.030) nM, respectively. Both high-affinity, low-capacity and low-affinity, high-capacity specific [3H]E2 binding were demonstrable. In conclusion, we could demonstrate that human osteoblast-like cells contain specific glucocorticoid receptors. In addition specific androgen, estrogen, and 1 alpha,25-(OH)2D3 binding was demonstrated. These cells contain several times more binding sites for dexamethasone than for dihydrotestosterone, estradiol, and 1 alpha,25-(OH)2D3, a feature that might contribute to the marked sensitivity of human bone to glucocorticoids.

Adult↗