Biomedical subjects

L Wang

Publications and source records attributed to L Wang.

At least 883 records · Page 49Linked to original sources

PubMed2000

[Operative therapy for lesions in insula].

OBJECTIVE: To probe into the significance of treating lesions in insula trans-sylvian approach in reducing cerebral injury in peri-tumor regions and post-operative complications. METHODS: All patients were operated on through fronto-temporal approach. The key procedure was that arachnoid membrane in the sylvian should not only be splited sharply under microscope but also be splited from the superface till to tumors in the insula. RESULTS: Post-operative CT showed no signs of cerebral injury. None of all the 15 patients suffered aphasia or memory change. CONCLUSION: Trans-sylvian approach is an effective operative way in treating lesions in insula.

Adult↗

PubMed2000

[Surgical correction of complete atrioventricular septal defect with tetralogy of Fallot].

OBJECTIVE: To report the surgical correction of complete atrioventricular septal defect with tetralogy of Fallot (AVSD-TOF). METHODS: Six consecutive patients aged 3 - 9 years underwent correction of complete AVSD-TOF. The two-patch technique for atrioventricular septal defect was used. The ventricular septal defect was closed through a right ventriculotomy and right atriotomy in each case. The commissure between the superior and inferior bridging leaflets of the left portion of the common atrioventricular valve was closed in each patient. RVOT obstruction was relieved by a transannular autologous pericardium with monocuspid valve. RESULTS: Postoperative complications included respiratory failure in 1 patient, low cardiac output syndrome in 1, and MOF in 1. There was one mortality because of MOF in the early postoperative period. Five survivors were followed up from 6 months to 5.5 years (mean 2.3 years). There was no late mortality and only one patient had mild left atrioventricular valve regurgitation. NYHA cardiac function was class I in 4 patients and class II in 1. CONCLUSIONS: AVSD-TOF can be corrected using the two-patch technique and closure of the ventricular septal defect through a combined approach using a right ventriculotomy and right atriotomy. Routine closure of the commissure of the left portion of the atrioventricular valve results in a low incidence of regurgitation. Good functional result can be achieved in most patients postoperatively.

Cardiac Surgical Procedures↗

PubMed2000

[Molecular cytogenetics of bone tumors].

OBJECTIVE: To study alterations of molecular cytogenetics of bone tumor serially. METHODS: Chromosomal analysis, immunohistochemistry, molecular hybridization, nested-PCR/SSCP, DNA sequence analysis, and gene transfection techniques were used to investigate the common bone tumors. RESULTS: Chromosomal aberrations were found in 50 cases of aggressive bone tumors, with a chromosome number aberration rate of 86.96% and a structure aberration rate of 93.94%. However, there were differences among different kinds of bone tumors. The incidence of chromosomal instability in 53 patients with bone tumors was higher than that in normal controls. Meanwhile, the fragile sites of chromosomes of the patients were closely correlated with the high nonrandom chromosome breakpoints of tumor cells and related oncogene loci. The data on p53 and Rb from 305 cases and 89 cases, respectively, indicated that abnormalities of p53 and Rb were common events in bone tumors. The accumulation of p53 protein and loss of Rb protein were more common in aggressive bone tumors. Structures and their products of related proto-oncogenes (ras, myc, fos, bcl-2, met) were studied in 124 cases. Bone tumors presented multi-oncogenes abnormalities with differences in different pathological kinds. Exogenous wild-type p53 gene transfected into osteosarcoma cell line induced growth inhibition and apoptosis of tumor cells. CONCLUSIONS: Abnormalities of chromosomes, proto-oncogenes, and tumor suppressor genes frequently exist in bone tumor cells and are correlated with oncogenesis.

Bone Neoplasms↗

PubMed2000

[Washing liquid cytology examination of operative wound in patients with breast tumor].

OBJECTIVES: To study the relation between exfoliated tumor cells in operative wound and clinical pathologic classification and to provide theory for killing exfoliated tumor cells in operation. METHOD: Washing liquid cytology examination of the operative wound was done in 262 patients with breast tumor. The relation between cytology examination and pathological classification was analyzed. RESULTS: In the 262 patients, exfoliated tumor cells were found in 63 patients, (24%). The detection rate in patients with I, II, III and IV stage was 5.3%, 27.1%, 79.3% and 100% respectively; The rate for T(1), T(2), T(3) stage was 6.1%, 18.9% and 51.3% respectively. The detection rate was 58.6% when the number of metastasis lymph node beyond 3; the rate was 14.3% and 14.1% respectively when the number below 3 or no lymphatic metastasis. CONCLUSION: The detection rate of exfoliated tumor cells is related to tumor advance, and it is necessary to kill exfoliated tumor cells in operation.

Breast Neoplasms↗

PubMed2000

[The relationship between effects and radiation doses of intra-arterial phosphorus-32 glass microspheres embolization therapy for patients with advanced liver cancer].

OBJECTIVE: To evaluate the relationship between effects and internal radiation of phosphorus-32 glass microspheres embolization therapy for liver cancer patients. METHODS: From 1994 to 1998, 44 patients with unresectable liver cancer received intra-arterial radio-embolization therapy using (32)P-GMS. Preoperative and postoperative function and energy level of the liver were tested by liver function test and arterial blood ketone body ratio (AKBR). CT, single photon emission computer tomography (SPECT), and AFP were used to judge the effect of the therapy; multivariate analysis was made. RESULTS: In the moderate dose group, low incidence of complication, high tumor shrinking rate and AFP decreasing rate, and long-term survival rate were observed. In the larger dose group, high incidence of liver failure, high tumor shrinking rate and AFP decreasing rate, and long-term survival rate were also observed. In the low dose group, low incidence of complication, but low tumor shrinking rate and AFP decreasing rate and long-term survival rate were not observed. CONCLUSIONS: The reasonable radiation doses for liver cancer patients may be about 30 Gy; if liver cirrhosis is serious, the doses can be reduced.

Adult↗

PubMed2000

[Re-surgical treatment for postoperative local recurrence of lung cancer].

OBJECTIVE: To evaluate the feasibility of re-surgical treatment for postoperative recurrence of lung cancer and its indications. METHOD: The clinical data of 20 patients treated again surgically after recurrence of lung cancer were analyzed retrospectively. RESULTS: The resectability, radical resectability, morbidity, and mortality were 75.0% (15/20), 80.0% (12/15), 20.0% and 0.0% respectively. The mean operative blood loss was 945 ml. The percentages of patients with pleural adhesion and intrapericardial dissection were 100.0% and 53.3% separately. The 1-year, 3-year and 5-year survival rates were 94.1%, 41.7% and 40.0%. The mean survival time of the patients receiving exploration or palliative resection was 15.0 months, shorter than the 56.9 months of the patients having radical resection. The mean survival time of the patients with N(0) was (105.3 +/- 82.0) months, much longer than (11.4 +/- 6.0) months of the patients with N(1) or N(2). CONCLUSION: Because of extensive adhesion, operations for postoperative recurrence of lung cancer are difficult, but if recurrence is found in time and operative indication is appropriate, re-surgical treatment can prolong the patients' survival time.

Adult↗

PubMed2000

[Effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice].

OBJECTIVE: To investigate the effects of p16 and GM-CSF combined gene therapy on immune response of tumor-bearing mice (Renca). METHODS: The model of renal tumor-bearing mice was constructed by subcutaneous injection of Renca cells. The tumor-bearing mice were divided into 5 groups at random three days after injection of Renca cells. Different groups of mice were given different treatments. The antitumor immune responses and the survival time of mice were tested. Histopathologic analysis of tumor tissue was done. RESULTS: Enhancement of antitumor immune responses and prolongation of survival period were observed more obviously in tumor-bearing mice after combined therapy than in those of the other four groups. CONCLUSIONS: p16 and GM-CSF combined gene therapy can enhance the antitumor immune responses of mice and inhibit the growth of pre-established renal cell carcinoma.

Adenoviridae↗

PubMed2000

[Spectrum of mycotic keratitis in China].

OBJECTIVE: To investigate the spectrum of mycotic keratitis in China. METHODS: We scraped and evaluated 615 mycotic keratitis in microbiology laboratories of Zhengzhou (central China), Shijiazhuang (north China) and Guangzhou (south China) in the twenty-two years between Jan. 1, 1975 and Jun. 30, 1997. RESULTS: Organisms of 18 genuses and 615 species isolated from corneal ulcers were found. Fusarium species were isolated the most frequently (65.0% in Zhengzhou, 33.3% in Shijiazhuang and 39.2% in Guangzhou), followed by Aspergillus (20.5% in Zhengzhou, 21.7% in Shijiazhuang, 30.7% in Guangzhou), Penicillium (3.6% in Zhengzhou, 11.6% in Shijiazhuang) and Curvularis (13.1% in Guangzhou). Between Jan. 1, 1975 and Dec. 31, 1986, Aspergillus species were most commonly isolated in Guangzhou (48.5%), followed by Fusarium (27.7%) and Penicillium (14.27%). CONCLUSIONS: Just as different regions of the world are characterized by specific endemic infections, the predominating organisms in corneal fungal infections also vary throughout the world. These regional differences in causative organisms in mycotic keratitis are clinically important because they influence the initial and more definite therapy. This study shows that Fusarium species are the most common organisms of mycotic keratitis in China.

Aspergillus↗

PubMed2000

[Residual risk research of HIV infection after blood screening in one county in China].

OBJECTIVE: To estimate the risk of HIV infections from blood transfusions, to evaluate existing blood screening measures, and to make recommendations to improve current blood screening practices. METHODS: Research was conducted in a county blood station. A statistical model and retrospective method were used to analyse the data. RESULTS: Between June 1995 and March 1998, 19 blood donors tested positive for HIV antibodies and among them, 7 were repeat blood donors. Twenty - two blood recipients were identified from these 7 donors during six months, 9 are still alive today, while one patient was infected with HIV from a blood transfusion while undergoing an operation. The residual risk of the blood station using a statistical model and retrospective methods were 1/1,773 - 1/28,867 and 1/2,008 - 1/2,730 donations respectively. CONCLUSION: The residual risk of HIV after screening in the county blood station was high. We recommend further improvements to current blood screening practices.

AIDS Serodiagnosis↗

PubMed2000

[The survey of HIV prevalence among drug users in Guangxi, China].

OBJECTIVE: To understand the distribution and related risk factors of HIV infection among drug users in Guangxi. METHOD: Three hundred and twenty five drug users were tested for serum HIV antibody, with enzyme linked immunosorbent assay. RESULTS: One hundred and sixteen drug users (35.7%) were found to be HIV positive. The main risk factors associated with HIV infection were intravenous drug users and needle sharing among drug users. The HIV prevalence was 40.9% (114/279) among IDUs, and only 4.3% (2/46) among oral drug users. The phenomenon of sharing injecting equipment (76.7%) among intravenous drug users in Guangxi represents a very serious problem. CONCLUSION: There is a high risk of HIV infection among drug users in Guangxi. Prevention programs urgently need to be implemented.

Adolescent↗

PubMed2000

[A case control study on the distribution of apolipoprotein AI gene polymorphisms in the survivors of atherosclerosis cerebral infarction].

OBJECTIVE: To investigate the frequency of the restrict fragment length polimorphisms (RFLP) at 2 MspI sites in the 5' - end of apolipoprotein AI (ApoAI) gene and its potential interaction to atherosclerotic cerebral infarction (ACI) in Chinese Han population. METHOD: Polymerase chain reaction (PCR) technique was used in a sample of 199 cases with documented ACI and 204 healthy matched by age and sex individuals selected from Chinese Han nationality in Beijing. The studied loci include promoter region (-75 bp) and the intron - 1 (+83 bp). RESULTS: (1) Both ACI and control groups M1 + + and M1 + - were major frequent genotypes in Beijng area, M2 + + were major frequent genotypes in intron - 1; (2) M1 + - genotype appeared more common among patients than in controls (0.487 vs 0.368, P < 0.05, OR = 1.64); (3) Individuals with M1 + -/M2 + + haplotype were significantly increased in patients compared with controls (0.437 vs 0.314, P < 0.05, OR = 1.70). CONCLUSION: It is suggested that both M1 + - genotype and M1 + -/M2 + + haplotype of ApoAI gene might associate with ACI in our study.

Apolipoprotein A-I↗

PubMed2000

[The epidemiological study of HIV infection among paid blood donors in one county of China].

OBJECTIVE: To observe the prevalence of HIV infection among paid blood donors and risk factors. METHODS: Selected the residents between 18 to 50 years old from two villages in one county of China in 1997. We observed the prevalence of HIV infection and keep following them to 2000. RESULTS: Nine hundred sixty-three residents were investigated and the prevalence of HIV was 1.8% (17/963). There were 88 paid blood donors among 963 interviewed subjects, the HIV prevalence was significantly higher (17.0% or 15/88) than those who didn't have the experiences of paid blood donations (0.2% or 2/875). Only one person out of 668 who were followed from 1997 to 2000 has converted to sero-positive, the incidence of HIV infection is 0.06 per 100 person-years. Nearly half of sero-negative and sero-positive people have the experiences of muscle injection. Among those with HIV-positive spouse, we found no one converted to sero-positive. The CD(4) count of those sero-positive people declined every year. HIV subtype and C2-V3 region sequence analysis were made for five HIV-positive people in 1998, the result indicated that all of them were infected with HIV-1 subtype B' and the genetic divergence rate was 2.4%. CONCLUSION: There was a high prevalence of HIV among paid blood donors in some regions, and paid blood (plasma) donations were the main risk factor for HIV infection. Although no evidence suggests that during the follow-up period the epidemic of HIV infection has spread locally, it still shall take measurements to prevent further transmission of HIV through paid blood donors.

Adolescent↗

PubMed2000

[The first discovery of endemic Lyme disease in Shandong province].

OBJECTIVE: To investigate the endemic area of Lyme disease in Shandong province. METHODS: An investigation on endemic Lyme disease was conducted by means of serological and etiological methods in Shandong province from 1992 to 1999. RESULTS: Of 1919 forestry residents, 120 residents had a significant antibody titer against Borolo burgdorferi strain B(31). The prevalance rate of Lyme disease was 6.25%. Haemaphysalis longicornis was a prevailing species of Ixodidae in the region. Spirochetes were observed in the midguts of 50 H. longicornis in this area by direct fluorescence antibody method. The overall positive rate was 12.0% (6/50). Two strains (TSH(1), TSH(3)) of spirochetes were isolated from H. longicornis. Both TSH(1) and TSH(3) were positively responded to McAb H(5332) and H(9724), but negatively to H(6831). Spirochetes were observed in the kidneys of 46 Rattus in this area by direct fluorescence antibody method with a positive rate of 13.26% (6/46). CONCLUSION: It was the first time that the mountainous areas in Shandong province was confirmed endemic areas of Lyme disease.

Animals↗

PubMed2000

[Genetic epidemiological study on non-insulin dependent diabetes mellitus].

OBJECTIVE: To study the general genetic pattern of non-insulin dependent diabetes mellitus patients. METHODS: 1,608 children were investigated for NIDDM family history, and 280 nuclear families were collected. RESULTS: The prevalence rates of NIDDM among first-degree relatives (2.38%), the parents (26.00%), the siblings (2.44%) and the offsprings (1.24%) were higher than that in general population respectively. The s/q was 10.17 by Penrose method, which was close to 1/q(1/2). The p(0) was 0.0244 by simple segregation analysis, which was lower than 0.10. The heritability of NIDDM was 0.54, using the Falconer Threshold Model. CONCLUSION: NIDDM has a familial aggregation, but not fit to the mono-genetic models. NIDDM has the feature of multifactorial inheritance.

Adult↗

PubMed2000

[Analysis of hepatitis G virus genotypes in some areas of China].

OBJECTIVE: To study the genotype distribution of hepatitis G virus (HGV) isolated from different population in some areas of China. METHODS: A total of 12 strains of HGV were isolated from different areas of China (three from Guangxi Zhuang Autonomous Region, three from Hebei Province, one from Heilongjiang, Jiangxi, Guangdong, Jiangsu, Anhui, Jilin Provinces and Xinjiang Autonomous Region each). The HGV 5' untranslated region (UTR) of the 12 isolates was sequenced and compared with other 26 isolates previously reported (17 from China and nine from other countries). RESULTS: The nucleotide sequences of five HGV clones isolated from the same patient with HGV infection were identical, with the homology of 99.1% - 100%, indicating that variation of HGV in the same human body was very limited. Genotyping could be based on the sequence of one positive clone isolated from a patient with HGV infection. The homology of the nucleotide sequences in the 12 isolates was 96.8% - 99.4%, suggesting that they belonged to the same genotype. The homology of the 12 isolates was 88.0% - 89.7%, 87.2% - 90.3% and 94.7% - 96.3%, respectively, as compared with HGV genotypes 1 (U36380), 2 (U44402) and 3 (D90601), which indicated that all the 12 HGV isolates belonged to genotype 3. Phylogenetic distance analysis of the 38 isolates suggested that HGV strains isolated from China could be further divided into different subgroups. CONCLUSION: The majority of Chinese HGV isolates belonged to genotype 3 and they could be further divided into different subgroups.

Base Sequence↗

PubMed2000

[p16, p53 and c-erbB-2 gene expression in bladder carcinoma].

OBJECTIVE: To investigate the expression of p16, p53 and c-erbB-2 proteins in bladder carcinoma and to analyze their correlation with tumor grade, clinic stage and metastasis. METHODS: Immunohistochemical method was used to detect the p16, p53 and c-erbB-2 gene expressions in 75 cases of bladder carcinoma. RESULTS: Positive rates of p16, p53 and c-erbB-2 in 75 cases of bladder cancer were 41.3%, 44.0% and 40.0%, respectively. p16 and c-erbB-2 gene expressions were related to the tumor grade and clinic stage (P < 0.05); p53 and c-erbB-2 gene expressions were related to the clinic stage and metastasis (P < 0.01). Oncogenes and/or antioncogenes altered expressions were found in 77.3% cases of bladder carcinoma, and oncogenes and/or antioncogenes with multiple altered expressions were found in 53.3% cases. CONCLUSION: The analysis of multiple oncogene and/or antioncogene is more valuable than that of single oncogene and/or antioncogene. The altered expression of oncogene c-erbB-2 and antioncogenes p16 and p53 might play a promotive role in the tumorigenesis and development of bladder tumors.

Adult↗

PubMed2000

[Subcutaneous panniculitis-like T-cell lymphoma: expression of cytotoxic-granule-associated protein TIA-1 and its relation with Epstein-Barr virus infection].

OBJECTIVE: To study the clinicopathologic features, the expression of cytotoxic-granule-associated protein TIA-1 and Epstein-Barr virus (EBV) infection in subcutaneous panniculitis-like T-cell lymphoma (SPTCL). METHODS: Totally 17 cases of SPTCL were studied. Immunohistochemical staining was done for TIA-1, CD45RO, CD3, CD20 and CD68, and in situ hybridization for EBV encoded small nuclear RNA (EBER1/2). RESULTS: In the 17 cases of SPTCL, the ratio of male-to-female was 1:1.1 and the median age was 24 years old. The main clinical manifestations were asymptomatic plaques, nodules and masses. Histologically, the pattern of lymphoid cells infiltration was panniculitis. The infiltrated lymphoid cells were pleomorphic and their mitotic figures were few (0 - 2/hpt). In the tumor tissue, there might be epithilioid granulomas, multinuclear cells, beanbag cells, small pieces of necrosis and small vessels with lymphoid cells infiltration in the vascular wall. Immunohistochemically, all the cases showed a T-cell phenotype and the expression rate of TIA-1 was 100% (16/16). By in situ hybridization, the positive rate of EBV-EBER1/2 was 29.4% (5/17). CONCLUSIONS: SPTCL has a specific histopathologic picture. It may originate from cytotoxic T lymphocytes or NK cells. Some of them may be accompanying with EBV latent infection.

Adolescent↗

PubMed2000

[Pathological features of liver biopsy from 66 cases of long term blood donors with chronic hepatitis C].

OBJECTIVE: To study the pathological features of liver biopsy from long-term blood donors with chronic hepatitis C. METHODS: From 250 long-term blood donors with positive serum anti-HCV antibody for more than five years, 66 donors with abnormal ALT level were selected and enrolled in this study. Liver biopsy was performed on these cases and the specimens stained with HE, and reticulin. Modified Schemer's classification for grading and staging was used during pathological observation. Twenty biopsies with serum HCV-RNA levels higher than 5.0 Meq/ml (bDNA) were studied immunohistochemically (SABC) for NS5. RESULTS: All cases displayed mild pathological changes. Grade 1 (G(1)) inflammation was 4.8% (3/63), G(2) 63.5% (40/63), G(3) 28.6%(18/63), G(4) two case. Stage 1 (S(1)) fibrosis was 60.3% (38/63), S(2) 34.9% (22/63), S(3) 3.2% (2/63), S(4) only one case. The main lesion was periportal inflammation, such as bile duct damage (100.0%), dense lymphoid aggregates (82.5%) and piecemeal necrosis (89.5%). Intralobular injury of hepatocytes was rather mild. Bridging necrosis and steatosis were not common and observed in only 12.3% (8/66) and 9.2% (6/66) respectively. As for fibrosis, proliferation and deposition of collagen were mainly distributed in the portal and perisinusoidal areas. Among 20 specimens with serum HCV-RNA levels higher than 5.0 Meq/ml (bDNA), all bile duct epithelial cells were positive for HCV-NS5 antigen, while hepatocytes in 4 cases were negative. CONCLUSION: (1) The pathological changes in this series show overall similarity with that of patients with posttransfusion hepatitis C. The main damages are portal and periportal inflammation, such as lymphoid aggregates, bile duct damage, piecemeal necrosis, and extensive periportal and perisinusoidal fibrosis. Intralobular damage was mild with occasional steatosis, which may be related to malnutrition, low level of serum iron due to long term donation. (2) Epithelial cells of the bile duct may be of another site for virus replication, thus it is related with bile duct damage and periportal inflammation.

Adult↗