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Biomedical subjects

L Robinson

Publications and source records attributed to L Robinson.

At least 127 records · Page 7Linked to original sources
PubMed1986

An experiment regarding crystallization of soluble proteins in the presence of beta-octyl glucoside.

Twenty-one soluble proteins, five tRNAs, and three protein-nucleic acid complexes were studied in a systematic manner with regard to their crystallization behavior from polyethylene glycol and ammonium sulfate solutions in the presence of 0 to 1.5% beta-octyl glucoside. Our observations suggest that this neutral detergent does influence in a very positive way the growth characteristics of the macromolecules included in this experiment. In general, more reproducible and rapid growth was noted with an increased number of large individual crystals at the expense of microcrystals. In several cases, new crystal forms were discovered. Selected x-ray diffraction analyses imply that crystals grown in the presence of beta-octyl glucoside diffract as well or better than those grown in its absence. In addition, a screen of two proteins grown in the presence of 14 different common detergents suggested that a general detergent effect may be beneficial for the growth of crystals of biological macromolecules.

Crystallization↗
PubMed1986

The future of psychiatric/mental health nursing.

The future of psychiatric/mental health nursing will reflect expansion of services to the elderly and chronic mentally ill. Providers of services and their reimbursement will also expand. If the specialty is to survive, changes in the undergraduate nursing curriculum must be addressed so that a larger cohort of nurses select graduate education in psychiatric/mental health nursing.

Aged↗
PubMed1984

Biochemical and x-ray diffraction analysis of concanavalin B crystals from jack bean.

Parallel biochemical and crystallographic studies have been carried out on the protein concanavalin B from Jack Bean (Canavalis ensiformis). The studies show the protein to be a monomer of 33,000 daltons with unexceptional amino acid composition and no covalently bound carbohydrate. The molecule contains a single, firmly bound zinc ion and we present evidence that the protein specifically binds nucleotide coenzymes including at least NADPH and flavin mononucleotide. An electron density map of the concanavalin B crystals (space group P6(1), a = b = 80.9 A, c = 102.2 A) has been calculated from phases based on six isomorphous heavy atom derivatives. A description of the structure of the protein based on the 5.0 A resolution x-ray diffraction study is provided. The structure appears to contain a substantial amount of alpha-helix as well as an extensive beta-sheet and these are organized into two domains of unequal size. The position of the bound zinc is indicated by anomalous difference Fouriers and the nucleotide binding site by conventional difference Fourier maps.

Journal Article↗
PubMed1984

Statistical association between cancer incidence and major-cause mortality, and estimated residential exposure to air emissions from petroleum and chemical plants.

An ecologic study design was used to investigate the relationship between exposure to air emissions produced by the petroleum and chemical industries, and average annual cancer incidence and major cause mortality rates among whites in Contra Costa County, California. Estimates for the exposure to major industrial sources of sulfur dioxide, hydrocarbons and oxides of nitrogen were used to subdivide the county by level of exposure to petroleum refinery and chemical plant emissions. Cancer incidence and major cause mortality rates were then calculated for whites in each of the exposure areas. In both males and females, residential exposure to petroleum and chemical air emissions was associated with an increased incidence of cancer of the buccal cavity and pharynx. In males, age-adjusted incidence rates for cancers of the stomach, lung, prostate and kidney and urinary organs were also associated with petroleum and chemical plant air emission exposures. In both sexes, we found a strong positive association between degree of residential exposure and death rates from cardiovascular disease and cancer, and a less strong positive association between exposure and death rates from cerebrovascular disease. There was also a positive association in men for deaths from cirrhosis of the liver. Although these observed associations occurred across areas of similar socioeconomic and broad occupational class, confounding variables and the "ecologic fallacy" must be considered as possible explanations. In particular, the stronger findings in men suggest an occupational explanation of the cancer incidence trends, and the effect observed in cirrhosis mortality suggests that lifestyle variables such as alcohol consumption were not adequately controlled for. While the public health implications of our findings remain unclear, the evidence presented is sufficient to warrant follow-up studies based on individual data in which possible biases can be more readily controlled.

Air Pollutants↗
PubMed1983

Legacy of fire.

Explore the source record for details and available documents.

Burns↗
PubMed1983

Influence of perinatal malnutrition on adult physiological and behavioral reactivity in rats.

A series of experiments examined the behavioral and pituitary-adrenal response to novelty of perinatally malnourished rats tested as adults after nutritional rehabilitation begun at weaning. Neither the behavioral measures of ambulation, rearing and defecation, nor the plasma corticosterone response to a brief exposure to an open field differentiated the previously malnourished subjects from controls. Similar to controls, previously malnourished subjects were also capable of displaying a graded corticoid elevation to environments increasingly different from the home cage. However, exploratory behavior, as measured by head-dip frequency and duration in the hole-board, was reduced in the previously malnourished rats. Although latency and amount of fluid consumed in a novel environment did not differ, previously malnourished rats were unable to use the cues associated with a consummatory behavior to modulate the pituitary-adrenal response to novelty. Thus, perinatal malnutrition does not influence either the behavioral or physiological activational response to novel stimulation but appears to alter the ability of the animal to use a consummatory behavior to modulate this response.

Animals↗
PubMed1982

Biochemical and crystallographic data for phaseolin, the storage protein from Phaseolus vulgaris.

We have isolated and biochemically characterized a major seed protein from Phaseolus vulgaris (common garden green bean) that appears to be identical with a form of the storage protein of that plant seed known as phaseolin. We have further shown that it appears very similar in most properties to the storage protein from Canavalia ensiformis (jack bean) which we have solved to 3.0-A resolution using X-ray diffraction techniques. [McPherson, A., & Rich, A. (1973) J. Biochem. (Tokyo) 74, 155-160; McPherson, A., & Spencer, R. (1975) Arch. Biochem. Biophys. 169, 650-661; McPherson, A. (1980) J. Biol. Chem. 255, 10472-10480]. We have crystallized phaseolin and conducted a preliminary X-ray diffraction analysis on it as well. The data show the crystals to be of pseudo cubic space group P432, although the symmetry can be, strictly speaking, only P1. The unit cell has one trimeric molecule of 150 000 daltons in a unit cell of dimensions a = b = c = 66.6 and alpha = beta = gamma = 90 degrees. The crystals apparently possess some form of disorder that makes reconciliation of the unit cell contents with the observed crystallographic properties difficult, although they do diffract strongly to better than 2.8-A resolution. No evidence of twinning has been observed.

Amino Acids↗
PubMed1982

The use of an animal drawing test in the assessment and disposition of transsexualism.

Reported results of a study that compared two projective techniques for a group of 19 transsexuals who were seeking sex reassignment surgery. The Draw-A-Person Test (DAP), widely used in clinical settings, was contrasted to the Animal and Opposite Drawing Technique (AODT), which is a relatively new and little-examined variation of the DAP. Discussion focuses on the use of both measures as predictors of those transsexuals to be recommended for surgery. Use of the AODT as an effective measure in the assessment of transsexualism is investigated.

Adult↗
PubMed1982

Psychiatric liaison nursing 1962-1982: a review and update of the literature.

Psychiatric liaison nursing, first conceptualized in the early sixties, evolved from a perceived need to address the psychosocial problems experienced by patients who were hospitalized for treatment of physical illness. Early practitioners tended to be educators and clinical supervisors who collaborated part-time with psychiatric liaison services associated with departments of psychiatry. Consultation with nursing staffs provided indirect care to liaison patients. Papers of the later sixties and early seventies described role models and delineated hierarchy, accountability, function, and supervision. Articles also began to appear about specific patient phenomena and liaison nursing interventions. The mid-seventies yielded a proliferation of position descriptions and the introduction of educational programs to prepare psychiatric liaison nurses at the master's level. The late seventies and early eighties have seen some elaboration of theory-based practice and further evolution of the liaison role. Research data have been cited and problems identified for ongoing study.

Humans↗