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Biomedical subjects

L Missotten

Publications and source records attributed to L Missotten.

At least 127 records · Page 7Linked to original sources
PubMed1987

Immunocytological study of phlyctenular eye disease.

Scrapings from phlyctens and conjunctiva of 12 patients with phlyctenular keratoconjunctivitis were studied using OKT4-Leu3a, OKT8, B1, BA1, S-100 and HLA-DR monoclonal antibodies. T-lymphocytes were present in both conjunctival and phlyctenular scrapings. OKT4-Leu3a positive cells outnumbered the OKT8 positive cells in both conjunctival and phlyctenular scrapings. B1 and BA1 positive cells were absent from the conjunctival scrapings, but were present in the phlyctenular scrapings. S-100 positive cells were present in both conjunctival and phlyctenular scrapings. However, they were very few in the conjunctival scrapings. Most of the cells in both conjunctival and phlyctenular scrapings were HLA-DR positive. These findings support the hypothesis that cell mediated immunity is responsible for the pathogenesis of phlyctenular eye disease.

Adolescent↗
PubMed1987

Displaced small amacrine cells in the retina of the marine teleost Callionymus lyra L.

The displaced small amacrine cells (DSA cells) in the dorsal pure cone part of the retina of the marine teleost Callionymus lyra have been analysed in a combined light and electron microcopical study. These unistratified cells have their dendritic arborization at 70% of the depth of the inner plexiform layer (P5 level). The DSA cells constitute a dense population and have variable dendritic field sizes. The bipolar input occurs in the P5,1 and the P5,2 pattern layer. The short, central DSA dendrites make ribbon synapses with midget mixed di-cone bipolar cells and with two types of pure cone bipolar cells. The amacrine input and output occurs in the fibrous layer that separates both pattern layers. The dendritic arborization is most extensive and the dendrites of neighbouring DSA cells are interconnected. The thick, central DSA dendrites are presynaptic to adjacent DSA cells and possibly to large bistratified and diffuse ganglion cells. The fine, peripheral DSA dendrites receive input from neighbouring DSA cells and probably from large uni- and bistratified and diffuse amacrine cells. A matching population of regularly placed small amacrine cells (RSA cells) has been observed. Their unistratified dendritic arborization is situated at 20% of the depth of the inner plexiform layer. The synaptic relations of RSA cells have not yet been completely analysed in detail. However, results up till now indicate that they most probably receive input from two bipolar cell types, one of which may be a pure cone type. In addition, the large bistratified amacrine and ganglion cells may be synaptically connected to the RSA cells as well as to the DSA cells.

Animals↗
PubMed1987

Cytological and immunohistochemical study of the limbal form of vernal keratoconjunctivitis by the replica technique.

The cellular composition of the inflammatory infiltrate present in 13 patients with the limbal form of vernal keratoconjunctivitis was examined in the conjunctival scrapings and the limbic replicas by means of Giemsa stain and immunohistochemistry. Conjunctival scrapings showed the presence of mast cells, lymphocytes, plasma cells, polymorphonuclear leucocytes, and very few basophils in all the specimens. Eosinophils were present in only four scrapings. The superficial epithelium of the limbic lesion and the adjacent cornea and conjunctiva was studied by the replica technique. The limbic lesion area showed the presence of necrotic epithelial cells mixed with inflammatory cells, including eosinophils, mast cells, lymphocytes, plasma cells, and polymorphonuclear leucocytes and very few basophils. Most of the inflammatory cells were HLA-DR+. Many OKT6+ cells were present, indicating the presence of Langerhans cells. T-lymphocytes including a few helper/inducer cells and many suppressor/cytotoxic cells, were detected in the infiltrate. In addition many B-lymphocytes were observed. These findings suggest that other immune mechanisms in addition to type 1 reaction are involved in the pathogenesis of the disease.

Adolescent↗
PubMed1985

Effect of topical acyclovir therapy on experimental herpes simplex keratouveitis.

Stromal keratitis and associated iritis were produced in rabbit eyes with the intrastromal injection of live herpes simplex virus. Treatment with 3% acyclovir and 2% trifluridine eye ointments was initiated one day after infection and continued for five consecutive days. These therapies significantly suppressed the development of keratouveitis compared with a placebo ointment. The suppressive action of trifluridine ointment on keratouveitis was significantly more pronounced than that of acyclovir ointment.

Acyclovir↗
PubMed1985

Prolonged survival of allogeneic corneal grafts in rabbits treated with topically applied cyclosporin A: systemic absorption and local immunosuppressive effect.

The modes of action of topical cyclosporin A were studied in rabbits. Immunorejection of corneal allografts was provoked by placing the grafts eccentrically, in contact with the limbus. Topical application of cyclosporin A five times daily for 28 days prevented the rejection of corneal allografts. All grafts were rejected in the control animals. The seven rabbits of the cyclosporin A group were subsequently treated for six months with a lower dosage of cyclosporin A 1%. In six rabbits the graft remained clear. One rabbit treated with two drops a day showed an allograft reaction that could be suppressed by increasing the dosage. After six months, discontinuation of the therapy resulted in rejection of all grafts within four weeks. Cyclosporin A could be detected in the plasma and aqueous humour of both eyes at the end of the treatment, raising the question whether the immunosuppressive effect of topically applied cyclosporin A was due to local or systemic action. Cyclosporin A 1% was therefore applied to the fellow eye five times daily following transplantation, and this treatment, producing similar plasma levels of cyclosporin A, failed to delay the rejection of eccentric corneal allografts. Consequently the suppression of the allograft rejection by topical cyclosporin A is primarily a local immunosuppressive effect, though systemic influence is not ruled out.

Administration, Topical↗
PubMed1985

Lymphocytes and Langerhans cells in the normal human cornea.

The distribution of B- and T-lymphocytes, of OKIa positive cells, and of HLA-ABC antigens in the normal cornea was investigated using monoclonal antibodies. The lymphocytes and Langerhans cells are present mainly in the well-vascularized limbic region but also occur albeit in small number in the center of the cornea. HLA-ABC antigens are strongly expressed on the epithelial cells of the cornea and the limbus.

Aged↗