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Biomedical subjects

L Missotten

Publications and source records attributed to L Missotten.

At least 199 records · Page 11Linked to original sources
PubMed1978

Histology and histochemistry of the normal superficial corneal epithelium of rabbit.

The superficial epithelial cells of normal cornea are in a process of gradual metabolic shutdown. Nuclear DNA may be depolymerized. Loss of DNA also occurs by extrusion in small quantities into the cytoplasm. There is a small quantity of RNA in these cells. The cytoplasm shows the presence of some vicglycols. The superficial corneal epithelial cells do not keratinize.

Animals↗
PubMed1978

Histopathology of human superficial herpes simplex keratitis.

In vivo corneal replicas were made in 20 cases of patients with superficial dendritic ulcers of the cornea. Histopathological study of the replicas and superficial epithelial cells showed that the dendrites are composed of rounded epithelial cells and variable sized syncytia containing bizarre shaped nuclei. Pseudopodia-like processes containing DNA and some RNA extend from the syncytia into the surrounding epithelial cells, which on coming into contact with these processes become rounded and liquefied to give rise to another syncytium. The epithelial cells adjacent to the dendrite and elongated and usually orientated parallel to the long axis of the lesion. Surrounding the terminal bulbs, they are disposed in an arcuate fashion. These cells show C-mitotic lesions, intranuclear and cytoplasmic inclusion bodies, and polykaryocyte formation. Microscopic examination of the corneal replicas shows the intranuclear lesions and rounding of cells up to about 2 mm away from the dendritic ulcers. These areas appear normal on clinical examination.

Cornea↗
PubMed1978

Cytology of the superficial keratinised cells in experimental keratitis sicca.

Keratoconjunctivitis sicca was produced in 2 rabbits by the surgical extirpation of the tear-secreting glands and the nictitating membrane in one eye. Flat mounts of the superficial corneal epithelium were prepared by the in vivo replica technique. Histomorphological and cytological studies of the keratinised cells showed the irregularly arranged, crumpled and piled-up cells. The cells contained coarse granular and fibrillar material in the cytoplasm and large granules in the nuclei. The reaction for -S-S- bridges was positive in these cells. They showed birefringence under polarisation microscopy but possessed some ribonucleoproteins.

Animals↗
PubMed1977

The replica technique used to study superficial corneal epithelium in vivo.

We used 2.15 to 2.3% collodion in amyl acetate to form a replica of the corneal surface epithelium in vivo in seven rabbit eyes and ten human eyes. When it was peeled off the replica removed superficial epithelial cells in patches. The corneal epithelium regenerated within one week. Repeated applications at weekly intervals for three months produced no harmful long-term effects in five rabbit corneas as evaluated with slit-lamp biomicroscopy, histological methods, and vital staining of the endothelium. In ten human eyes, the technique was harmless and chemically cauterized the dendritic herpetic ulcers. We studied the replicas and cells by conventional histological methods and electron microscopy. The preservation of cells was fairly good even for ultrastructural study.

Animals↗
PubMed1976

A duplex retina and the electroretinogram in the nocturnal Perodicticus potto.

The presence of cones in potto's retina has been proved beyond doubt although they are very restricted in number (1 cone for 300 rods). Morphologically, speaking there is no point in calling these cones "rudimentary" except for their slender outer segment. There are red sensitive elements in that retina at wavelengths beyond the spectral sensitivity of visual purple and it is tempting to assume that these elements are cones. The ERG evoked from these elements by red light differs from that in response to white and blue light. They dark-adapt faster than the receptors sensitive to blue and white flashes. However in some of their properties, for example fusion frequency, these cones behave like rods in other species. As these few cones seem to activate the bipolar cells nearly as effectively as the numerous rods, it is suggested that these cones may be responsible for day vision in the potto.

Animals↗