[Chinese herbal medicine and cytoprotection in gastrointestinal tract].
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Animals↗
Biomedical subjects
L Li
Publications and source records attributed to L Li.
Preparation of monoclonal antibodies against chicken liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase and their effects on enzyme activities.
The effects of the monoclonal antibodies (McAbs) directed against chicken liver 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase (6PF-2-K/Fru-2, 6-P2ase) on the structure and function of the enzyme were studied. Using chicken liver 6PF-2-K/Fru-2, 6-P2ase as antigen, 7 clones of monoclonal antibodies specifically binding with the antigen were obtained. The epitopes of the antigen recognized by the 6 McAbs localized on the fructose-2, 6-bisphosphatase domain of chicken liver 6PF-2-K/Fru-2, 6-P2ase, and the other (H2) are on the 6-phosphofructo-2-kinase domain. All of the 7 McAbs could activate the kinase activity of the bifunctional enzyme by twofold and had a similar effect on the bisphosphatase activity of the bifunctional enzyme which resulted in a fourfold increase of the bisphosphatase activity of the bifunctional enzyme. However, the McAbs did not affect the activity of the separated fructose-2, 6-bisphosphatase domain. The results suggested that the Fru-2, 6-P2ases in the bifunctional enzyme and in the separated bisphosphatase domain were in two different conformation and activity states.
Animals↗
[Reversal of adriamycin or vincristine resistance by tetrandrine in human cancer cells in vitro].
In an in vitro culture system of human cancer cells MCF-7 and its adriamycin-resistant line MCF-7/Ad or KB and its vincristine-resistant line KBv200, tetrandrine was found to exhibit significantly selective anticancer activity against the drug-resistant cancer cell MCF-7/Ad. In addition, essentially complete reversal effects of tetrandrine on drug resistance were observed in MCF/Ad or KBv200.
Alkaloids↗
[Patterns and distribution of the spontaneously discharges neurons in the nucleus paragigantocellularis lateralis of rats].
Using extracellular recording technique, we systematically explored the spontaneously discharging neruons in the caudal half of nucleus paragigantocellularis lateralis (cPGCL) in anesthetized and spontaneously breathing Sprague-Dawley rats. The discharge patterns of the neurons and the distribution of the respiratory related neurons (RRNs) in this PGCL area were analysed. A total of 237 neurons with spontaneous discharge were stably recorded in 14 rats. Among them 39 were RRNs and 198 non-RRNs (NRRNs). The discharge patterns of the 198 NRRNs were primarily tonic (n = 178). Of the 39 RRNs, 24 were inspiratory (INs), 12, expiratory (ENs), and 3, phase spanning (PSNs). The INs could be further classified into augmenting (n = 20) and constant or bellshaped (n = 4) INs, and the ENs, into decrementing (n = 7) and constant or bell-shaped (n = 5) ENs. The phase spanning neurons showed a constant or bell-shaped pattern of discharge, including inspiratory-exspiratory (IENs, n = 2) and expiratory-inspiratory (EIN, n = 1) phase spanning ones. Most of the RRNs were distributed in the dorsolateral portion of the cPGCL area and tended to concentrate in the more caudal part. These results suggest that PGCL is one of the important neural substrates responsible for the regulation of rhythimic respiration.
Animals↗
103 cases of postpartum uroschesis treated by acupuncture at huiyang point.
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Acupuncture Points↗
[The effect of RU486 on contractility of rabbit oviduct smooth muscle].
An investigation on the effect of RU486 on isolated ovicluct smooth muscle contraction of pseudopregant rabbits was carried out by using the mechanical-electrical transducer of Galson recorder. The results showed that: (1) RU486 acted directly on the oviduct smooth muscle by increasing contractile frequency, without changing tension and amplitude. This effect is similar to that found in the in vivo experiment. (2) RU486 partially inhibited Ca(2+)-induced smooth muscle contraction and also showed significant synergistic effect of Verapamil and antagonistic effect of NE on muscle contractility. However, RU486 is unable to exert any effect on Forskolin-stimulated contraction. Thus it appears that the RU486 effect on oviduct smooth muscle contraction is a result of affecting intracellular free calium, due possibly to interference of Ca2+ influx and/or endoplasmic reticulum Ca2+ release and Ca(2+)-Ip3 transducing mechanism.
Abortifacient Agents, Steroidal↗
[Clinical evaluation of Doppler ultrasonic angiography].
The diagnosis of carotid surgery is based on angiography. 1607 Doppler cases of extracranial carotid Doppler ultrasonic angiography were examined. Among them, 1527 had remote cerebral stroke and 285 had carotid endarterectomy. The results indicated that the group of carotid endarterectomy conformed to Doppler ultresonic angiograph of preoperation and were consistent with 10 positive and 10 negative cases of random sampling. The method has advantages of easy operation, time-saving, low expenses, no complication and reliable diagnosis to use Doppler ultrasonic angiograph in carotid surgery. It was worth spreading in non-invasive arterial morphology and hemodynamics.
Adult↗
A molecular pathologic study on apoptosis in retinoblastoma and the mechanism of spontaneous regression in retinoblastoma.
OBJECTIVE: The present study was designed to prove the existence of apoptosis in retinoblastoma (Rb) and to determine the pathogenic mechanism of spontaneous regression of Rb as well as the relationship between them. METHODS: Qualitative morphological study on Rb was performed by means of light microscope, electron microscope and TdT mediated biotin-dUTP nick-end labeling (TUNEL). Quantitative study was performed by automatic image analysis technology (AIAT) stained with Feulgen reaction. RESULTS: The characteristic regressed area occurred in all 47 cases of Rb. Morphological changes observed within Rb closely resembled the apoptotic cell described by Kerr et al in 1972. Under electron microscope, details of apoptosis were observed in 7 cases of Rb: the morphological sequence of events occurred in and around the cell nucleus. The morphology of the TUNEL labeling Rb cell was various. TUNEL labeling showed more positive cells in regressed area, while fewer in advanced area. AIAT revealed that apoptosis index (AI) in regressed areas was higher than that in other areas of Rb, DNA average ploidy (DP) consisted with the histology grade of Rb, the degree of hyperdiploid (DH) in metastatic area was distinctly higher than that in other areas (P < 0.05). CONCLUSIONS 1. Morphologic evidence proved the existence of apoptosis, especially more in regressed area of Rb. 2. Apoptosis may participate in the spontaneous regression of Rb. Apoptosis contributed to the spontaneous regression of Rb. 3. Tumor growth parameters of Rb (AI, DP, DH) obtained by AIAT may be used as quantitative index for pathologic classification, the selection of clinical treatment and the prognostic evaluation.
Apoptosis↗
[Automatic image analysis of argentophil protein granule quantitative measurement on nucleolus area of limbus epithelial neoplasms].
OBJECTIVE: To study the pathological diagnostic significance of argentophil protein (AgNOR) quantitative analysis at nucleolus area of limbus epithelial neoplasms. METHODS: Automatic image analysis system was used for argentophil protein granule measurement in the pathologic diagnosis of 45 cases with limbus epithelial neoplasms, including benign hyperplasia, atypical hyperplasia, carcinoma in situ and squamous cell carcinoma. RESULTS: In various lesions, there were the differences in the distributive pattern, size and area of AgNOR granules, which were closely related to the nature of the neoplasms (benign or malignant) and its cell differentiation. CONCLUSION: Automatic image analysis of AgNOR quantitative measurement on limbus epithelial neoplasms may indicate the proliferative activity and cell differentiation in various lesions, help to differentiate benign or malignant neoplasms and effectively enhance the level of pathologic diagnosis.
Carcinoma in Situ↗
[Report of clinical use of fixed-removable prosthesis].
Thirty-two patients provided with the fixed-removable prosthes is were followed up 3 month to 2.5 years. A total of 52 abutments were included, 17 of which were telescopic retainer, 14 were bar attachment retainer, 4 were rod attachment, 12 were stud attachment and 5 were slide attachment. Few technical failures were recorded. All found the prosthesis satisfactory and well functioning.
Adult↗
[Functional evaluation of the mitochondria from Warthin tumor].
The mitochondria were isolated from 8 surgical specimens of Warthin tumor of the parotid and the activity of the respiration was evaluated. The average respiratory control rate (RCR) of the mitochondria of the tumors (1.12 +/- 0.50) was lower than that of the normal mitochondria (2.10 +/- 0.75). The preliminary experiment showed a loosely coupled state of oxidative phosphorylation. It indicates that the hyperplasia of the mitochondria in the Warthin tumor results from the metabolic disturbance of the tumor and it is a compensation in the cellular level.
Adenolymphoma↗
[Detection of p53 gene mutation in bronchial biopsy samples from lung cancer patients with polymerase chain reaction-single strand conformation polymorphism-silver staining method].
In order to diagnose lung cancer at gene level, bronchial biopsy specimens from lung cancer patients, who were diagnosed by pathologic method using biopsy specimens from the same site, were used for detection of p53 gene mutation by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP)-silver staining method. 15 of 25 (60%) specimens were found to be positive. In 10 small cell lung cancer specimens, 7 were positive; in 15 non-small lung cancer specimens, 8 were positive. These results coincided with those of other reports using surgical specimens and sequencing method. Clinical analysis showed no correlation between SSCP positivity and the patients' clinical data such as age, sex, smoking habit, stage of tumor at the time of diagnosis. It is concluded that a small piece of bronchial biopsy specimen could be used to detect p53 gene mutation instead of surgical specimens and this method might be used as an adjunct to cancer screening or for a gene diagnosis prior to gene therapy. In comparison with the routine radionuclide labelled method, silver staining method has the advantage of being simple, quick, economic, safe and convenient for clinical use.
Biopsy↗
[Radioembolization with 32P-labelled glass microspheres for advanced hepatocellular carcinoma].
We evaluated the efficacy and side effect of 32P-Labelled glass microspheres (32P-GMS) as a radioembolizer for patients with advanced hepatocellular carcinoma (HCC). 24 patients with unresectable HCC received internal radiation treatment of 32P-GMS. The tumor size varied from 3.6 to 18 cm. Hepatic arterial embolization was carried out through intraoperative or Seldinger's method. The mean absorbed radiation dose of the liver was 3250 rad (range from 1200 rad to 8000 rad). The radiation intensity within the tumor was 3.3 times stronger than in liver tissue. Not significant bone marrow renal toxicity was noted within 1 to 3 months. > 50% of tumor shrinkage was found in 17 cases, and < 50% of tumor reduction in 5 cases. The cumulative survival rate of 3, 6, 12, 18, 24 months was 92%, 75%, 54%, 33% and 29%. Hepatic arterial instillation of 32P-GMS appears to be safe and effective for unresectable HCC even with portal vein thrombosis.
Adult↗
[Clinicopathological study on patterns of cervical lymph node metastases from squamous cell carcinomas (SCC) of the head and neck].
In order to investigate the patterns of cervical lymph node metastases from head and neck SCC, serial sections were performed on 384 radical neck dissection (RND) specimens. Positive lymph node was found in 60.4% RNDs. The cervical lymph node spread from SCC in the head and neck regions including oral cavity, oropharynx, hypopharynx and larynx has some predictable patterns, i.e., for primary SCC of the oral cavity, the majority of cervical lymph node metastases were clustered at levels I, II and III; and for primary carcinoma of the oropharynx, hypopharynx and larynx, a majority of node metastases were located at levels II, III and IV. The positive lymph nodes mainly distributed at only one level or consecutive levels. The rates of pathologically positive lymph node and extranodal spread grew with the increase of the clinical N-staging. It is suggested that supraomohyoid neck dissection (levels I, II and III) is particularly applicable to carcinomas of the oral cavity, and lateral neck dissction (levels II, III and IV) is applicable to carcinomas of the oropharynx, hypopharynx and larynx in patients with limited (N0 and N1) neck nodules, but for patients with N2 and N3 nodules, RND is neccessary to eradicate the nodal metastases. Moreover, the postoperative radiotherapy is indispensable for ruling out the occult cervical lymph node metastaese in selective neck dissection.
Adult↗
[Rules of the absorption, distribution and translocation of nitrogen, phosphorus and potassium in turmeric plant].
It has been found out that the percentages of nitrogen (N), phosphorus (P) and potassium (K) in turmeric plant (Curcuma longa) are higher in young organs. Varying with the growth stages, the nutrient contents of the plant, whose amount order is K > N > P are the highest in the seedling stage. Nutrient absorption occurs mainly in the young turmeric forming stage and enriching stage. Nutrient translocation takes place mainly from leaves and leaf stalks to young and old turmeric, the rate of P being the highest.
Nitrogen↗
[Curcuma longa L. tuber yield simulation model and its application under combined agronomic measures for good-quality, high-yield and obvious economic results].
A design of second order orthogonal rotative regression was developed and field tested, and the tuber models for yield, quality and benefit were simulated and built. The effect of agronomic measures on the tuber yield is plant density > phosphorus > sowing date > nitrogen > potassium. There is a clear interrelation effect among agronomic measures. The results of simulation experiment have shown that the optimal combination of farming practices can greatly increase the tuber yield, quality and benefit, and is adjusted according to climate, soil, ferilizer or manure sources, and so on.
Computer Simulation↗
[The absolute configurations and biological activities of alpha- and beta-anordrins].
The molecular structures and absolute configurations of alpha- and beta-anordrins are reported. Animal experiments showed that pure alpha-epimer possessed high anti-implantation and anti-early pregnancy effects, but the pure beta-epimer exhibited very low effects at the same dose. MNDO program was applied for the optimization of the configurations of epimers to establish the isolated molecular configurations and calculate the quantum chemical indexes. The results showed that the configurations and the differences of action field in three-dimensions of alpha- and beta-epimer are probably the factors that the alpha-epimer exhibited biological activity.
Animals↗
[Patterns of systematic development for the resources of traditional Chinese drugs and herbs in the Three Gorges Reservoir areas].
Based on the comprehensive survey, the status and countermeasures of systematic development for the resources of traditional Chinese drugs and herbs in the rural exploitative emigration and eco-agricultural economy in the Three Gorges Reservoir Areas are put forth constructively.
China↗