Biomedical subjects

L Li

Publications and source records attributed to L Li.

At least 937 records · Page 52Linked to original sources

PubMed1999

[The significance of detection of hepatitis C virus RNA in peripheral blood mononuclear cells of patients with chronic hepatitis C].

OBJECTIVE: To determine the role of peripheral blood mononuclear cells (PBMCs) in hepatitis C virus (HCV) infection. METHODS: HCV RNA, HCV antigen and Fas antigen were detected in PBMCs of 22 patients with chronic hepatitis C, 21 renal dialysis patients with anti-HCV positive and 12 health blood donors as normal controls. All specimens were studied by electronic microscope (EM). RESULTS: (1) The positive rate of HCV RNA in PBMCs of 22 patients with hepatitis C was 77.3% (17/22). (2) EM showed that HCV particles replicated in PBMCs of the patients with HCV infection, but not in the serum of patients with negative HCV RNA and healthy controls. (3) HCV RNA were positive both in the serum and PBMCs of 8 patients with HCV particles positive and had a close relationship with HCV antigen and Fas antigen. The expression of HCV and apoptosis were found in the cytoplasm of PBMCs in these 8 patients. CONCLUSION: (1) Hepatitis C virus could replicate in PBMCs and produce infectious HCV particles. (2) HCV in PBMCs may act as the source of reinfection for HCV and lead to persistent infection and recurrence.

Adult↗

PubMed1999

[Treatment of severe acute renal failure with continuous renal replacement therapy].

OBJECTIVE: To present the experience in 101 severe acute renal failure (ARF) patients treated with continuous renal replacement therapy (CRRT) in our hospital from May 1986 to January 1999. METHODS: Patients'age, sex, previous health status and characteristics of renal failure were recorded. The acute physiology and chronic health evaluation II (APACHE II) score were calculated before CRRT treatment. RESULTS: 60 (59.4%) patients survived the acute phase of their illness, and 41 (40.6%) patients died. In the survival group, there were significantly less patients with pre-renal causes of renal failure and refractory course of uremia, low APACHE II score before treatment and less need of mechanical ventilation or vasoactive supporting. No difference was found in the percentage of oliguria between the survival and total group. CONCLUSION: CRRT is the treatment of first choice in ARF, especially in those complicated cases with severe parachymal renal diseases. The patients' age, previous health status, APACHE II score before CRRT, delayed occurrence of acute renal failure, need of mechanical ventilation and vasoactive drugs were predictors of a worse prognosis in severe ARF patient. The prognosis of renal type ARF patients improved with CRRT.

Acute Kidney Injury↗

PubMed1999

[A study on the reserve capacity of endothelial tissue plasminogen activator in patients with acute coronary syndrome].

OBJECTIVE: To study the changes of the endothelial tissue plasminogen activator (tPA) reserve capacity in patients with acute coronary syndromes (ACS). METHODS: Veinous blood was sampled for determination of markers of the fibrinolytic system and hypercoagulability at admission and on the 10th day in patients with acute coronary syndrome (ACS), including acute myocardial infarction (AMI) in 15 and unstable angina pectoris (UAP) in 20. An occlusion test to determine the maximal endothelial tPA release was performed in order to evaluate the endothelial tPA reserve capacity. 20 patients with stable angina pectoris (SAP) and 12 normal subjects served as control. RESULTS: Patients with ACS had higher levels of tPA mass concentration, PAI-1 activity, D-dimers mass concentration and TAT mass concentration at admission and on the 10th day as compared with those in SAP and normal subjects. Maximal endothelial tPA release was initially reduced to (2.2 +/- 0.8) microg/L (P < 0.01, vs patients with SAP), but the levels recovered on the 10th day of follow-up to (4.5 +/- 1.2) microg/L (P > 0.05, vs patients with SAP). CONCLUSION: Endothelial tPA reserve capacity is reduced only during the acute phase and becomes normalized during follow-up. The alterations of plasma markers of systemic fibrinolysis persist longer in patients with ACS. The results demonstrated that the intravascular thrombus formation may relate more to the disturbance of endothelial fibrinolysis than to markers of the systemic fibrinolytic system. It is very important to correct the disturbances of endothelial fibrinolysis during the acute phase of ACS.

Acute Disease↗

PubMed1999

[The application of intraoperative cholangiography in laparoscopic cholecystectomy].

OBJECTIVE: To evaluate the role of intraoperative cholangiography in laparoscopic cholecystectomy (LC). METHODS: Intraoperative cholangiography was attempted in 1 466 patients, and was successfully completed in 1382 patients. The success rate was 94.27%. RESULTS: Stones in the common bile duct in 92 patients, bile duct injury in 8 patients and abnormal bile duct in one patient were found. CONCLUSIONS: Intraoperative cholangiography is an accurate and safe procedure, can provide clear films of biliary tree, and has a high success rate. Its application can reduce the rate of bile duct injury and improve the quality of LC.

Bile Ducts↗

PubMed1999

[Retroviral-mediated transfer of a hygromycin phosphotransferase-thymidine kinase fusion gene into human bladder carcinoma cell].

OBJECTIVE: To evaluate the therapeutic efficacy of retroviral-mediated hygromycin phosphotransferase-thymidine kinase fusion gene (HyTK)/GCV on human bladder carcinoma cell. METHODS: A retroviral expression vector pL (HyTK) SN was constructed. By using FuGENE 6-mediated transfection and "ping-pong effect" technique, high-titer of retroviral supernatant was obtained and HyTK gene was transferred into EJ cells. A retroviral vector encoding, enhanced green fluorescent protein, EGFP was used to rapidly detect the transduction efficiency. Antitumor effects were observed after GCV treatment. RESULTS: In vitro experiments demonstrated the EJ cells transferred by HyTK gene were killed in the GCV treatment. Non-transduced parental cells were not sensitive to GCV, but they were dead by the bystander killing of neighboring cells when mixed with EJ/HyTK cells at various ratios. In addition, this not only affect wild-type EJ cells but also cells from different bladder carcinoma cell lines. CONCLUSIONS: Retroviral-mediated HyTK/GCV systems were a promising suicide gene therapy for bladder carcinoma. EGFP may act as a convenient and rapid reporter to monitor retroviral-mediated gene transfer and expression in bladder carcinoma cells.

Antiviral Agents↗

PubMed1999

[The effect of extracting lens on damage of corneal endothelium in vitreous surgery].

OBJECTIVE: To observe the effect of extracting lens on the damage of corneal endothelium in vitreous and retinal surgery. METHODS: The corneal endothelial cell densities of 179 eyes were observed preoperatively and at postoperative 2, 4, 8 weeks and 3 and 6 months. RESULTS: Postoperatively, there was no significant change of the corneal endothelial cell densities compared with that of the preoperative ones in phakic eyes among different operations. There was also no significant change of the cell densities, but an tendency of cell loss in the aphakic and pseudophakic eyes after vitreous and retinal surgery. The corneal endothelial cell densities decreased significantly after vitrectomy with lensectomy. CONCLUSIONS: It is the vitrectomy with lensectomy that makes the corneal endothelial damage. Vitreous and retinal surgery poses a risk to corneal endothelium in the aphakic and pseudophakic eyes.

Adolescent↗

PubMed1999

[Histopathology studies on 8,673 cases of ocular adnexal hyperplastic lesions and tumors].

OBJECTIVE: To study the histopathologic classification of ocular adnexal hyperplastic lesions and tumors according to the histogenesis and the anatomic sites. METHOD: To analyze retrospectively the pathologic specimens of 8,673 cases of ocular adnexal hyperplastic lesions and tumors between 1961 - 1997. RESULTS: There were 7,265 benign cases (83.77%), and 1,408 malignant cases (16.23%). Classification by anatomic sites: eye lid 5,313 cases (61.26%), orbit 1,484 cases (17.11%) and epibulbar 1,876 cases (21.63%). Classification by histogenesis: (1) Primary tumor 6,679 cases: epitheliogenic 2,481 cases (37.15%), mesenchymal 753 cases (11.27%), lympho-hematopoietic 102 cases (1.53%), neurogenic 1,542 cases (23.09%), congenital abnormal embryogenetic 1,767 cases (26.46%), uncertain resource tumor 34 cases (0.50%). (2) Secondary tumor 13 cases; (3) Metastatic tumor 9 cases; (4) Non-tumor lesion 1,972 cases. CONCLUSION: The classification is helpful to the histopathologic diagnosis and the study about the detailed distribution of common ocular adnexal hyperplastic lesions and rare tumors, and it also increases the pathologic diagnostic data in North China.

Eyelid Neoplasms↗

PubMed1999

[Relationship between obese gene expressive product and simple obesity in children].

OBJECTIVE: To investigate the relationship between obese gene expressive product-obese protein (OP) and the pathogenesis and prognosis of simple obesity in 30 children with normal body weight (control group). METHODS: Before immunoassay, OP was extracted from the whole blood samples with Sep-pak C(18) Cartridge. RESULTS: The plasma contents of OP in the obese group were (118.53 +/- 25.02) ng/L and (197.14 +/- 26.83) ng/L (P < 0.001), respectively. In the obese group, plasma OP was not detected in three cases and was (19.90 +/- 3.94) ng/L in other three cases. These obese children with severe OP deficit not only had more increased body mass index, and levels of total cholesterol, triglyceride and low-density lipoprotein-cholesterol, but also had lower therapeutic effect (16.67%) than those with mild OP depletion (79.16%). CONCLUSION: The children with simple obesity have OP deficit. Simple obesity may be related with the reduced plasma content of OP. The obese children with severe OP deficit have poorer curative effect and unfavorable prognosis.

Child↗

PubMed1999

[Expression of endothelin 1 and tumor necrosis factor alpha in injuried renal tubules and their influences on renal interstitial fibroblasts].

OBJECTIVE: To study the expression of endothelin 1 (ET-1) and tumor necrosis factor (TNFalpha) in the epithelial cells of normal and injured renal tubules and their influences on renal interstitial fibroblasts. METHODS: Cultivation of renal tubular epithelial cells and renal interstitial fibroblasts and establishment of a renal tubulo-interstitial fibrosis (TIF) animal model; reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical staining, radioimmunoassay (RIA), double immunohistochemical; and (3)H-TDR incorporation techniques were applied to study the relationship between ET-1, plus TNFalpha and the proliferation of renal interstitial fibroblasts. RESULTS: Expression of ET-1 mRNA and TNFalpha mRNA from renal tubular epithelial cells was obtained (546 bp and 415 bp) respectively as well as the relevant ET-1 and TNFalpha proteins. The concentration of ET-1 and TNFalpha in the culture medium were 1.42 pg/ml and 0.58 ng/ml respectively. The amount of ET-1 and TNFalpha was increased during cell injury and regeneration. In addition, the ratio of (3)H-TDR incorporation and the expression of ET-R mRNA and TNF-R1 mRNA (545 bp and 347 bp) were markedly increased than that of the control group (P < 0.05) when ET-1 or TNFalpha was added in the culture media. CONCLUSION: Injured and regenerated renal tubular epithelial cells are able to synthesize and liberate more ET-1 and TNFalpha than that of the normal renal tubules. ET-1 and TNFalpha are also effective in promoting the proliferation of renal interstitial fibroblasts through ET-R and TNF-R.

Animals↗

PubMed1999

[Effects of cultivating measures on rhizome yield and some main active constituents of Curcuma longa L].

OBJECTIVE: To substantiate the effects of cultivating measures on the rhizome yield and main active constituents of Curcuma longa. METHOD: Randomized block design was applied to the study of the sowing time and plant density, and orthogonal experiment L9 (3(4)) was conducted to determine the quantity of fertilizer. RESULT: The effects of cultivating measures on the rhizome yield and some main active constituents of Curcuma longa were noticeable. The rhizome yield went up, and the contents of curcuminoids and essential oils went down with the increase of fertilizer application. CONCLUSION: The main cultivating measures for high-yield and good-quality of rhizome: rational sowing time-late March, plant population-12 checkrows per m2, 1 mother rhizome per checkrow. The suitable fertilizer requirements have also been formulated.

Curcuma↗

PubMed1999

[Effect of growth period, storage time and varieties on the contents of main active constituents of Curcuma longa L. in rhizome].

OBJECTIVE: To provide criteria for selection, storage and cultivation of the rhizome of Curcuma longa. METHOD: Field observation and sample analysis on the rhizome. RESULT: The contents of curcuminoids and essential oils in mother and daughter rhizome went up with the progress of plant development. The contents of curcuminoids reached the maximum in early September and early October. The contents of essential oils in mother rhizome reached the maximum in early September. The contents of curcuminoids and essential oils in the rhizome varied with the species and went down with the increase of storage years. CONCLUSION: The rhizome should be collected when leaves of the plant have just withered. The suitable storage time is three years.

Curcuma↗

PubMed1999

[A probe in good agricultural practice for traditional Chinese drugs].

OBJECTIVE: To provide some scientific reference for the good agricultural practice (GAP) for traditional Chinese drugs (TCDs). METHOD: Based on an integrated analysis of the factors bearing on the output and quality of TCD along with a study on the possible pollution sources of TCDs, some initial idea concerning the research and scope of GAP has been outlined. RESULT AND CONCLUSION: GAP for TCDs should be designed to incorporate large-scale production bases, standardization of drug varieties, optimization of planting techniques, rationalization of harvesting and processing, establishment of famous brands for quality TCDs, pollution-free production and circulation, efficient coordination of production, supply and marketing, etc.

Drugs, Chinese Herbal↗

PubMed1999

[A study on soil suitability for growth of rhizome of Curcuma longa L].

OBJECTIVE: To seek for the type of soil that influences favorably the yield and quality of Curcuma longa. METHOD: Field contrast control experiment, correlation analysis, path analysis and cluster analysis were used. RESULT: The rhizome yield and quality of Curcuma longa. varied greatly with the types of soil texture. There are clear correlations among the soil factors, as well as between the soil factors and the rhizome yield and quality of the plant. A regression equation of the rhizome yield and soil factors has been built up. CONCLUSION: The soil suitable for growing Curcuma longa. can be classified into three types according to the results of correlation analysis and cluster analysis.

Curcuma↗

PubMed1999

[The effect of cold- and heat-natured medicines on 5-HT and NE cells and nerve fibres in rat's brain, pituitary and adrenal].

OBJECTIVE: To study how cold- and heat-natured medicines act on 5-HT and NE cells in the brain, pituitary and adrenal of rats. METHOD: Based on immunohistochemical method, the specific anti-TH and anti-5-HT sera were used to observe the changes of 5-HT and NE cells in brain and adrenal as well as fibres in pituitary. The contents of these two transmitters in brain were also determined by fluoriphotometry. RESULTS: Cold-natured medicines made the staining of 5-HT cells deeper and NE cells lighter, while heat-natured medicines act by contraries. CONCLUSION: Cold-natured medicines increase the content of 5-HT in brain and decrease the content of NE, while heat-natured medicines work the other way around. The morphologic changes are consistent with the functional ones.

Adrenal Glands↗

PubMed1999

[Comparative study on livertaxis of lactosaminated human growth hormone and galactosyl-human growth hormone].

This study was conducted to compare the livertaxis of lactosaminated human growth hormone (hGH-L) with that of galactosyl human growth hormone (hGH-Gal), using radioactive tracer technique. hGH-L and hGH-Gal were first prepared. After hGH-L, hGH-Gal and hGH were labelled with 131I or 125I, the data on biodistribution in mice, imaging in rabbits and chicken were obtained. hGH-L and hGH-Gal had remarkable livertaxis and their percentages for uptake in liver were 68.83%-74.65% and 68.18%-74% respectively, which were about two times as high as that of hGH. All of the hepatic bindings were receptor-mediated. But, the synthesizing of hGH-L was more easy-to-do, more mild in reaction conditions, and more economical of raw material as compared with the synthesizing of hGH-Gal. Therefore, hGH-L is a more potential receptor-mediated hepatic targeting secreting somatomedian drug for treating dwarfism.

Animals↗

PubMed1999

[A clinical study on systemic inflammatory response syndrome and multiple organ dysfunction syndrome in patients with abdominal surgical emergency].

In order to analyze the development from systemic inflammatory response syndrome(SIRS) to multiple organ dysfunction syndrome (MODS) and probe the therapeutic strategies, the authors studied retrospectively the clinical data of SIRS and MODS in 1374 patients with abdominal surgical emergency. The results showed that on admission the morbidity of SIRS was 32.5%, afterwards the morbidity of MODS was 6.4%, and the mortality of the patients with SIRS was 4.8%. After 48 hours' therapy, MODS developed in 33.0% of the SIRS cases. Of all the 1374 patients, 40 developed MODS(2.9%) and 27 died (67.5%). The authors point out that the outcome of the patients with abdominal surgical emergency can be improved on condition that SIRS is early diagnosed, the cause of SIRS after 48 hours of therapy is well defined, and the body inflammatory response is properly regulated.

Abdomen, Acute↗

PubMed1999

[Significance of apoptosis status and apoptosis-associated antigen expression in human colorectal adenocarcinoma sequence].

To investigate the effects of apoptosis on colorectal tumorigenesis and its possible biological significance, the apoptotic frequency in primary cultural cell of 9 normal mucosa, 4 adenomas and 9 adenocarcinomas in time period of 2, 12, 24, 48 hour was measured by flow cytometry. The apoptotic cells index (AI) in situ for 15 colorectal normal mucosa, 7 hyperplastic epithelial, 25 adenomas and 77 adenocarcinomas was identified by the terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end labeling technique (TUNEL). Ki-67 proliferate index (KI), wafl and p53 genes were immunostained with ABC method. The results showed that culture related apoptotic incidence was obviously decreased in cultural tumor cell when compared with mucosa cell after 24-48 hour in vitro. There was a directly positive relationship between the spontaneous apoptosis and Ki-67-index in vivo. The well differentiated or early stage lesions with intensive bcl-2 expression were significantly more likely to have low AI and KI. Both mp53 accumulation and wafl depression which mainly related to KI, had no apparent correlation with AI, bcl-2/bax expression and clinicopathological features statistically; elevatory bax/bcl-2 and pervasive wafl depression led to an increasing AI/KI both in adenoma with atypia and in advanced cancer with distant metastasis or embolus, comparatively. The data indicated that the reduction of susceptibility to inductive apoptosis may contribute to the early phase of tumorigenesis, that AI in vivo may reflect proliferative activity, and that bcl family was closely associated with spontaneous apoptosis and biological behavior of human colorectal cancer.

Adenocarcinoma↗

PubMed1999

[A study on fresh rhizome simulation model and its application to comprehensive agronomic measures for good quality and high yield of Curcuma longa L].

OBJECTIVE: To work out standardized cultivating measures for Curcuma longa according to the analysis of fresh rhizome simulation model. METHOD: Second order orthogonal rotative regression design. RESULT: The fresh rhizome simulation model was built up, and 5 main measures that affect fresh rhizome yield were analyzed by the mathematical model of five unknowns second order orthogonal rotative regression. Sowing time, plant population and potassium play an important role in raising the fresh rhizome yield. Sowing time is clearly interrelated with plant population, and plant population is clearly interrelated with the fertilizer used Sufficient N and K are necessary for raising the yield. There is a clear interrelation among the fertilizer factors. CONCLUSION: Optimal farming measures: sowing time March 1-17, and plant population-110,000 to 115,000 mother rhizomes per hm2. The optimal fertilizer requirements have also been formulated.

Computer Simulation↗