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Biomedical subjects

L Li

Publications and source records attributed to L Li.

At least 433 records · Page 24Linked to original sources
PubMed2001

[Expression of cytokeratin 7 and 20 in ovarian metastatic carcinomas].

OBJECTIVE: To study the distinctive clinicopathologic and immunohistochemical difference between ovarian metastatic carcinomas and primary ovarian carcinomas. METHODS: The clinical and pathological features of 27 cases of ovarian metastatic carcinomas (gastric carcinomas 12 cases, colon carcinomas 11 cases, others 4 cases) obtained from our department were reviewed. Immunostainings for CK (AE1/AE3), CK7, CK20, CEA, vimentin, nm23 were performed with SP staining methods. RESULTS: On gross examination, metastasis from gastric adenocarcinoma were usually bilateral, while solid (11/12) and metastases from colonic adenocarcinoma were more often unilateral and cystic (7/11). Microscopically, metastases from gastric adenocarcinoma revealed signet ring cells or poorly differentiated adenocarcinomas (12/12), whereas metastases from colonic adenocarcinomas showed similar morphology of endometrioid adenocarcinoma (8/11). The majority of ovarian metastases of gastric carcinoma (7/12) and colon carcinoma (8/11) were CK20 positive. In particular, CK20 was invariably expressed in colon cancer metastases. Most of the ovarian metastatic carcinomas from the gastrointestinal tract failed to react with immunostaining of CK7. A combined use of CEA, vimentin and nm23 had made a correct classification for 11/12 cases of the gastric carcinoma, 10/11 cases of the colonic cancer. CONCLUSIONS: CK7 and CK20 have been proved to be useful antibodies in distinguishing between metastatic carcinomas and primary carcinomas of the ovary. Combined use of a panel of antibodies can give more significant results.

Adenocarcinoma↗
PubMed2001

[Detection of the expression of Smad4, transforming growth factor beta(1) and beta receptor II proteins in paraffin-embedded human pancreatic cancer tissues].

OBJECTIVE: To explore the relationships between Smad4, TGF beta1 and TbetaR II in the TGF-beta pathway and the possible mechanisms by which they effect pancreatic carcinoma. METHODS: The expression of Smad4, TGF beta1 and TbetaR II in paraffin embedded pancreatic carcinoma tissues was detected by using antibodies against Smad4, TGF beta1 and TbetaR II with EnVision immunohistochemistry. RESULTS: The positive rates of Smad4, TGF beta1 and TbetaR II were 58.93% (33), 66.07% (37) and 60.71% (34), respectively. The positive rates of the above three proteins in matched normal pancreatic tissues were 89.29% (50), 25.00% (14) and 25.00% (14) respectively. There was a significant relationship between the expression of TGF beta1, clinical stage and the metastasis of the tumor (P < 0.05). There was also a significant relationship between the expressions of TGF beta1 and TbetaR II (P < 0.05). CONCLUSIONS: The expression of Smad4 in pancreatic carcinoma tissue is significantly decreased but the expression of TGF beta1 and TbetaR II are increased. Smad4 may play an important role in the regulation of TGFbeta inducible gene expression and subsequent growth inhibition, but TGFbeta may also act in a Smad4-independent manner.

DNA-Binding Proteins↗
PubMed2001

[Investigation on the life of Wu Jutong, the famous physician of seasonal warm diseases, and his books].

The name of Wu Jutong, a physician of seasonal warm diseases in the Qing dynasty was Tang, another name being Peiheng, and Jutong was his alias. He was born in 1758 and died in 1836 and his native place was now Huai'an city, Jiangsu province. Wen bing tiao bian (Treatise on Differentiation and Treatment of Seasonal Warm Diseases) was not finished in 1798, but 1813, after a hard efforts of 15 years. He compiled a Yi yi bing shu (A book Checking the Defects of Medical Professionals) in 1931 and arranged his medical records as books in 1833 to help latercomers. He not only researched medicine carefully and thoroughly and was brave in innovation, but also was an honest man with high morality. He opposed factions when studying, and had an open mind, collected widely from various schools, and had right attitude towards his own achievements. He was a model, and worthy to study and yearn.

Books↗
PubMed2001

Nucleotide sequences of the coat protein and readthrough protein genes of the Chinese GAV isolate of barley yellow dwarf virus.

The nucleotide sequences of the coat protein (CP) and readthrough protein (RTP) genes of the Chinese GAV isolate of Barley yellow dwarf virus (BYDV) were determined. The CP and RTP genes of GAV isolate comprised 600 and 1374 nucleotides, respectively. When the CP and RTP gene sequences of GAV isolate were compared with those of BYDV isolates MAV-PS1, P-PAV, NY-SGV and Cereal yellow dwarf virus RPV (CYDV-RPV), the highest similarity (97.2%) between the CP genes of GAV and MAV-PS1 isolates was observed, while the RTP genes of these two isolates shared a lower similarity (87.8%). The results of the alignment of the deduced amino acid sequences of RTP showed that the sequence diversity observed was located at the C terminus.

Amino Acid Sequence↗
PubMed2001

Anomalous (preduodenal) portal vein: autosomal recessive mutation in AKR/J mice.

BACKGROUND AND PURPOSE: Anomalous (preduodenal) portal vein was found in AKR/J mice. It is a rare congenital malformation in humans, and to the authors' knowledge, has never been reported in laboratory animals. Morphology, clinical signs of disease, and heritability of this anomaly were examined. METHODS: Fifty-three strains of inbred mice (6,026 mice) in our mouse colony were examined for preduodenal portal vein and its association with clinical signs of disease (vomiting or abdominal pain) and other anomalies. Heritability also was tested by use of cross-backcross matings of AKR/J mice with clinically normal PT mice. RESULTS: The portal vein was found at the ventral side of the duodenum in most (98%) AKR/J mice, whereas it ran at the dorsal side of the duodenum in 52 other inbred mouse strains in our mouse colony. Clinical signs of disease and other congenital anomalies were not detected in this strain of mice, although position has a high association with other congenital anomalies in humans. Heritability testing of this anomaly in AKR/J mice indicated single autosomal recessive inheritance. CONCLUSIONS: Preduodenal portal vein found in AKR/J mice is a single autosomal recessive mutation, but is not associated with clinical signs of disease and other congenital malformations.

Animals↗
PubMed2001

Changes in the contents of glycogen and lactate in the brain and blood during hypoxic preconditioning.

Mice were randomly divided into groups H4 (hypoxic preconditioning group with repetitive hypoxic exposures for four runs), H(1) (hypoxic control group with exposure to hypoxia for one run) and H(0) (normal control group with no exposure to hypoxia). Glycogen content of whole brain of group H4 was found to be significantly higher than that of groups H(1) and H(0). The glycogen content in telencephalon, diencephalon and pons of group H4 was markedly higher than that in the corresponding areas of groups H(1) and H(0). Glycogen content of whole brain in group H(1) was markedly lower than that in group H(0), whereas no significant difference was seen in these brain subregions between groups H(1) and H(0). Brain lactate contents of groups H4 and H(1) did not show significant difference, though they were significantly higher than that of group H(0). Blood lactate content of group H4 was significantly lower than those of groups H(1) and H(0). The results above indicate that the concomitant increase of glycogen and decrease of lactate in the brain are due to the participation of aerobic metabolism during hypoxic preconditioning or the formation of tolerance to hypoxia.

Animals↗
PubMed2001

[Detection of HPV DNA and its relationship with peripheral T cell subsets in patients with condyloma acuminata].

OBJECTIVE: To study the relationship between presence of HPV DNA related sequence and cellular-immune responses in patients with condyloma acuminata (CA). METHODS: HPV DNA related sequences in biopsy material of 30 CA patients were determined by using Southern blot hybridization, and typed according to the restricted fragment length polymorphism (RFLP) analysis. At the same time, the T-cell subpopulations in peripheral blood of 30 patient with CA were also measured by using anti-CD3, CD4, CD8 monoclonal antibody labeled SERC direct rosette assay. RESULTS: HPV DNA positive results were revealed in 19 of 30 patients with CA(63.3%). Among them, 3 cases possessed HPV 6 DNA homologous sequence (15.8%), 15 had HPV 11 DNA homologous sequence(78.9%), and only one case had a sequence homologous to HPV 16 DNA(5.2%). The percentages of CD3 and CD4 cells were significantly lower (P< 0.01) in CA patients than those in controls. However the percentage of CD8 cell was strikingly higher (P< 0.001) in CA patients than that in controls. Consequently the CD4/CD8 ratio was noticeably lower (P<0.001) in patients with CA than that in controls. The decrease of CD3 cell and the lowering of C D4/CD8 ratio were closely related to the positivity of HPV DNA homologous sequence (P =0.008 02, P=0.004 17). Conclusion The presence of HPV DNA in the patients with CA results in inhibitory effects on a series of cellular-immune responses, which may play an important role in the pathogenesis of CA.

Adult↗
PubMed2001

[Studies on quality specification standards for Saposhnicovia divaricata (Turcz.) Schischk].

OBJECTIVE: To work out a qualitative-quantitative standard for Saposhnikovia divaricata. METHOD: TLC and HPLC were adopted. RESULT: Four chromone compounds were identified by TLC, and the contents of two chromone compounds were determined by HPLC. CONCLUSION: Prim-O-glucosylcimifugin, 4'-O-beta-D-glucosyl-5-O-methylvisamminol, sec-O-glucosylhamaudol and cimifugin should be present simultaneously in S. divaricata. The contents of prim-O-glucosylcimifugin and 4'-O-beta-D-glucosyl-5-O-methylvisamminol in S. divaricata should not be lower than 0.12% respectively.

Apiaceae↗
PubMed2001

[In vivo distribution and expression after skin injury of fibronectin splicing variants].

OBJECTIVE: In order to develop new criteria and to select positive controls for forensic medicine, experiments on in vivo distribution and expression after skin wound of fibronectin splicing variants, EIIIA and EIIIB were studied. METHODS: Rat tissues were homogenized to extract total RNA which was then amplified by RT-PCR technique. RESULTS: (1) EIIIA+ and EIIIB+ of normal skin were not expressed, whereas EIIIA+ after 18 hours' injury was expressed abundantly. No obvious charge of EIIIB+ was detected. The differences between results of using two methods of injury were not significant. (2) The distribution of fibronectin variants in different adult organs were in the following order: brain > heart > liver. CONCLUSIONS: EIIIA+ may be used as a sensitive criterion for research of skin injury.

Animals↗
PubMed2001

[Effect of para-aminobenzoic acid on the adhesion of Streptococcus mutans].

OBJECTIVE: To investigate the effects of Para-aminobenzoic acid (PABA) on the adhesion of Streptococcus mutans to saliva-coated hydroxyapatite (S-HA). METHODS: The adhesion model in vitro established by Gibbons was adopted in this study to quantify adsorptive cells through [3H] thymidine labelling. RESULTS: PABA solution over a range of concentration from 10(-9) to 10(-3) g/L could inhibit the attachment of S. mutans to S-HA effectively, and the inhibition declined when the concentration of PABA solution was 10(-3) g/L. CONCLUSION: PABA can inhibit the adherence of Streptococcus mutans to S-HA effectively.

4-Aminobenzoic Acid↗
PubMed2001

[A study of on the transport of neurotrophin-4 in facial nerve].

OBJECTIVE: This is a study on the transport information of neurotrophin-4 (NT-4) in facial nerve using radioactive tracer technique. METHODS: After one lateral facial nerve trunk of adult rabbit was transected, a silicone chamber was inserted between the stumps, and 7.4 MBq of 131I-NT-4 was administered into the chamber. At the distinct moment of post-injection, the head of one rabbit was imaged at coronary position, the bilateral facial nerve trunk and brain-stem of the others were collected and counted respectively. After 1 mg of brain-derived neurotrophic factor (BDNF) and 7.4 MBq of 131I-NT-4 were administered into the chamber of one rabbit, the rabbit was imaged at coronary position of head at distinct moment. RESULTS: 5.08% of 131I-NT-4 was transported into facial nerve trunk at 4 h of post-injection. 131I-NT-4 presented high peak in facial nerve trunk during the period of 8, 12 h, the peak values were 20.58% and 22.74% respectively. 34.75% and 45.57% of 131I-NT-4 were transported into the brain-stem of experimental side at 8 h and 12 h respectively. The transport of 131I-NT-4 was markedly restrained by BDNF in facial nerve. CONCLUSION: NT-4 displays a receptor-mediated retrograde transport in facial nerve.

Animals↗
PubMed2001

[The measurement of 153Sm-EDTMP bone uptake rate and its relationship with therapeutic effect].

OBJECTIVE: To evaluate the measurement of 153Sm-EDTMP Bone uptake rate with whole-body scintigraphy and analyze the relationship between bone uptake rate and therapeutic effect. METHODS: 66 patients with bony metastases were examined with whole-body scintigraphy 10 minutes and 6 hours after the administration of 153Sm-EDTMP, and then bone uptake rate was calculated. RESULTS: The bone uptake rate in 66 patients ranged from 31.9% to 86.6%, (mean 56.0%). The patients with higher bone uptake rate had better overall therapeutic effect. According to the bone uptake rate calculated by whole-body scintigraphy, the therapy activity administered within the limit of bone marrow absorbed dose of 1400 mGy in these patients was 1.40-2.27GBq (mean 1.90 GBq). CONCLUSION: The present authors have developed a whole-body scintigraphic technique which is simple and reliable in calculating prospectively a safe and effective dose of 153Sm-EDTMP for palliating the patient's painful bone metastases without myelotoxicity.

Adult↗
PubMed2001

[Investigation of the tongue squamous cell carcinoma prognostic factors].

OBJECTIVE: The aim of this study was to investigate the factors which influence the prognosis of the tongue squamous cell carcinoma (TSCC). METHODS: The Cox's proportional hazard model was used to analyze 121 cases of TSCC treated by the affiliated dental hospital of West China University of Medical Sciences in the period from 1990 to 1994. Thirteen parameters (age, sex, occupation, primary tumor site, clinical stage, tumor differentiation, clinical therapy, cervical lymph node metastasis, surgical margin status of the primary tumor, amount of blood transfusion, smoking, drinking, reconstruction during operation) were evaluated. RESULTS: The monofactor analysis showed that factors influencing the postoperative survival of TSCC included primary tumor site, the surgical margin status of the primary tumor and the cervical lymph node metastasis (P < 0.05). Multifactor analysis demonstrated that two of these thirteen relevant factors influenced the postoperative survival of TSCC independently. They are successively the surgical margin status of the primary tumor and the cervical lymph node metastasis (P < 0.05). CONCLUSION: This study suggests that it is critical for surgeons to resect the primary tumor and the metastatic cervical lymph nodes thoroughly to improve the survival rate of TSCC patients.

Adult↗
PubMed2001

[An experimental study on effects of pingyangmycin on vessels].

OBJECTIVE: The aim of this study was to investigate the mechanism of Pingyangmycin sclerotherapy for cavernous hemangiomas. METHODS: Totally 9 rabbits (one as the control) were selected to be injected with Pingyangmycin and sodium morrhuate into the auricularis posterior vein, and then these veins were examined histologically 2, 7, 14 and 21 days after injection respectively. RESULTS: After injection, Pingyangmycin nonspecifically made the endothelia and veins defective, and induced proliferation of endothelial cells and smooth muscle cells after 7 days. Further, the veins became sclerostenosis 21 days after injection. On the other hand, sodium morrhuate caused thrombosis quickly and the vascular cavity disappeared. Finally, the thrombus became fibrogenesis. The local skin could be observed swollen and necrosis. CONCLUSION: Pingyangmycin is a better sclerosant for the therapy of cavernous hemangioma.

Animals↗
PubMed2001

[A retrospective clinical study of 6539 cases of malignant oral-maxillofacial tumors].

OBJECTIVE: The aim of this study is to obtain the general information of post-operation malignant oral-maxillofacial tumor patients in forty-seven years. METHODS: A total of 6539 patients with malignant oral-maxillofacial tumors in the hospital of stomatology, west china university of medical sciences, from 1953 to 2000 were retrospected. RESULTS: The average age of patients with malignant oral-maxilloficial tumor is 48.7 years old. The ratio of male to female is 2.31:1. The most common original malignant tumors in this oral-maxillofacial region are malignant oral tumors, malignant maxillary tumors, malignant mandibular tumors, and malignant sialoma successively. As to the original tissues of tumors, there were 5869 cases of epithelial tumors, and made up 89.75% of all the investigated patients. Tumors came from lymphatic and hematopoietic systems and mesoderm were much less those came from epithelia. There were 4177 cases of squamous cell carcinomas and the percentage was 63.88%. CONCLUSION: The incidence rate of malignant tumors in oral-maxillofacial region tends to increase, and the average age of patients also rose. The ratio of male to female decreases gradually. Epithelial tumors, especially squamous cell carcinomas are very common tumors happening in this region.

Adolescent↗
PubMed2001

[Expression of vascular endothelial growth factor in oral squamous cell carcinoma].

OBJECTIVE: The aim of this study was to estimate the expression of vascular endothelial growth factor(VEGF) and intratumoral microvessel density (IMVD) in oral squamous cell carcinoma(SCC). METHODS: A total of 60 cases of oral squamous carcinoma were immunohistochemically stained with a SP method by using monoclonal antibodies of VEGF and CD34. RESULTS: There was no significant difference in the expression of VEGF in different TNM stages and lymphe node metastases of oral squamous cell carcinoma. The expression of VEGF showed no relation with pathologic grades, but IMVD expression differed significantly in SCC. The carcinoma in grade I and IV of IMVD differed significantly in expression of VEGF. CONCLUSION: It indicates the change of IMVD may resulted from the change of VEGF in oral SCC.

Adult↗
PubMed2001

[Effects of para-aminobenzoic acid (PABA) on growth of Streptococcus mutans].

OBJECTIVE: The aim of this study is to examine effects of para-aminobenzoic acid (PABA) on the growth of Streptococcus mutans (S. mutans). METHODS: Different concentrations of PABA (10(-10)-10(-3) g/L) were separately transferred to modified Carlsson medium. S. mutans (ATCC 25175) grew in modified Carlsson medium with different concentrations of PABA. All cultures were incubated at 37 degrees C anaerobically in an atmosphere of 80% of nitrogen (v/v), 10% of hydrogen (v/v) and 10% of carbon dioxide (v/v) for 48 hours. The absorbance values of S. mutans were measured by using a spectrometer (UV-1601). The colony forming units (CFU) were obtained by growing S. mutans in media with different concentrations of PABA (10(-10)-10(-3) g/L). RESULTS: Different concentrations of PABA had different stimulating effects on the growth of S. mutans (P < 0.05). But this kind of stimulating effects declined when the concentration of PABA was 10(-3) g/L. CONCLUSION: This experiment indicates PABA has stimulating effects on the growth of S. mutans, and PABA can promote growth of S. mutans.

4-Aminobenzoic Acid↗