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Biomedical subjects

L Gardner

Publications and source records attributed to L Gardner.

68 records · Page 4Linked to original sources
PubMedDate not supplied

Isolation of human extravillous trophoblast cells by attachment to laminin-coated magnetic beads.

An isolation method has been developed based on laminin-coated magnetic beads which yields human trophoblast with a high degree of purity. Immunostaining with a panel of monoclonal antibodies indicates that these trophoblast cells are mainly of the extravillous variety. The mechanism for selection may be due to the expression of surface laminin receptors by these cells. This procedure, therefore, can provide appropriate cellular targets for in vitro assays against potential uterine effectors in studies on the local trophoblast-decidual interaction.

Cell Separation↗
PubMedDate not supplied

Recognition of trophoblast HLA class I molecules by decidual NK cell receptors--a review.

During placentation the extravillous trophoblast (EVT) cells migrate through the decidua towards the maternal spiral arteries. The walls of the arteries are then destroyed by trophoblast resulting in an increased blood flow to the fetus. These EVT express HLA-G, HLA-E and HLA-C, an unusual combination of two non-classical and one classical MHC class I molecules. The decidua is infiltrated by distinctive uterine natural killer (NK) cells during the time of trophoblast invasion. These cells express a variety of receptors (CD94/NKG2, KIR and ILT) which are known to recognize HLA class I molecules. There is, therefore, a mechanism for molecular recognition of the placental trophoblast cells. The possible functional consequences of this uterine NK cell-trophoblast interactions are uncertain. One possible result is in an altered NK cell cytokine profile which modulates the invasive proclivity of the EVT. In this way placentation could be controlled.

Decidua↗
PubMedDate not supplied

Evaluation of a monoclonal antibody, BC-1, which identifies an antigen expressed on the surface membrane of human extravillous trophoblast.

This paper describes a new Mab BC-1 which is directed at a cell surface antigen expressed by extravillous cytotrophoblast and not by villous cytotrophoblast, so it is useful for distinguishing between the two populations. The antigen recognised by BC-1 is trypsin-resistant, which allows it to be used for flow cytometric analysis of living trophoblast isolated by enzymic disaggregation. However, BC-1 is not trophoblast-specific but cross-reacts with some other tissues, in particular endothelial cells and peripheral blood monocytes. The nature of this antigen has not been established.

Animals↗
PubMedDate not supplied

A prospective study of outcome from protein-energy malnutrition in nursing home residents.

To study recovery from protein-energy malnutrition in patients newly admitted to a chronic care facility, biochemical and anthropomorphic malnutrition indicators were measured prospectively over a 2-month period. Subjects were observed for a mean of 76 +/- 18 days after admission. Factors which might affect nutritional status including method of feeding, energy prescribed, use of supplemental feedings, assistance in eating, and amount of diet consumed were recorded. Confounding variables including illness, mental status, functional status, or prescription drugs were evaluated for their impact. On the basis of an empiric nutrition score, 54% of newly admitted residents were malnourished. Improvement in nutritional score occurred in 63% of malnourished patients remaining in the facility whereas 37% remained malnourished. Two related factors, percentage of diet consumed and degree of assistance required in eating, were important differences in the outcome of patients remaining malnourished. Interventional studies to determine whether outcome can be improved by more aggressive feeding methods or by feeding teams need to be undertaken.

Aged↗
PubMedDate not supplied

Ethical questions.

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Ethics Committees↗