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Biomedical subjects

L G Wayne

Publications and source records attributed to L G Wayne.

70 records · Page 4Linked to original sources
PubMed1967

Autolysis and secondary growth of Mycobacterium tuberculosis in submerged culture.

Mycobacterium tuberculosis has been found to exhibit autolysis and spontaneous secondary growth in modified Sauton medium. The phenomenon is a function of high glycerol concentration of the medium and limitation of air in test tubes. It is not a function of depletion of nutrients and is probably not a manifestation of lysogeny or spheroplast formation. The cycle of growth, autolysis, and secondary growth is related to development of lethal conditions during a period of severe oxygen limitation followed by a metabolic change associated with slower depletion of glycerol.

Bacteriolysis↗
PubMed1967

Selection of characters for an Adansonian analysis of mycobacterial taxonomy.

Tests which are most frequently employed to provide characters for numerical analysis of bacterial taxonomy provide little differentiation among the slow-growing members of the genus Mycobacterium. A special scheme was proposed to deal with this problem. Characters were based, to some extent, on quantitative reactions to tests, and were defined, for each test, in terms of natural clustering behavior of a large collection of assorted strains. Inter- and intrataxon analyses were based on comparisons of reaction patterns with tentative hypothetical median strain patterns. This permitted the analysis of larger numbers of cultures than can be practically studied in a conventional n x n table.

Catalase↗
PubMedDate not supplied

Numerical taxonomy and cooperative studies: roles and limits.

Because of the wide array of specialized tests needed for classification of mycobacteria and the complexity of some of these tests, the International Working Group on Mycobacterial Taxonomy was organized in 1967 to conduct cooperative studies and provide a broad data base for numerical taxonomic analysis of the genus. Certain parameters, such as immunologic properties and lipid composition, were withheld from the numerical analyses to provide independent corroboration of the observed clustering. Overall, these studies have yielded confirmation of the validity of many species, as well as detailed description of these species. The numerical analyses do not resolve all taxonomic questions in the genus Mycobacterium, but they do provide phylogenetic hypotheses based on phenetic clustering. These hypotheses presently are being tested by techniques for measuring DNA relatedness and protein sequence conservation. Concurrently, cooperative studies are continuing in order to provide adequate description of some of the more obscure mycobacterial species.

Mycobacterium↗
PubMedDate not supplied

Serological approaches for the characterization of catalase in tissue-derived mycobacteria.

Cell-free extracts of Mycobacterium lepraemurium from mouse liver and M. leprae from armadillo liver were analysed for the presence of any mycobacterial catalase by using the specific inhibitor 3-amino-1,2,4-triazole and seroprecipitation titrations. These studies clearly demonstrated the presence of a "T" type of mycobacterial catalase in M. lepraemurium and placed it, in terms of immunological distance, in a position between M. tuberculosis and M. avium. The results did not reveal any detectable "T" catalase activity in the M. leprae preparations. The "M" type catalase activity which was observed did not bind to antisera against "M" catalase of M. kansasii, but was bound to the extent of 80% to antisera against normal armadillo liver catalase. The significance of the component of the "M" catalase in M. leprae preparations which did not react against antibodies to normal liver remains to be determined.

Amitrole↗